Detailed transcriptome analysis of the plant growth promoting Paenibacillus riograndensis SBR5 by using RNA-seq technology

Fernandes de Brito L, Irla M, Kalinowski J, Wendisch VF (2017)
BMC Genomics 18: 846.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Background The plant growth promoting rhizobacterium Paenibacillus riograndensis SBR5 is a promising candidate to serve as crop inoculant. Despite its potential in providing environmental and economic benefits, the species P. riograndensis is poorly characterized. Here, we performed for the first time a detailed transcriptome analysis of P. riograndensis SBR5 using RNA-seq technology. Results RNA was isolated from P. riograndensis SBR5 cultivated under 15 different growth conditions and combined together in order to analyze an RNA pool representing a large set of expressed genes. The resultant total RNA was used to generate 2 different libraries, one enriched in 5′-ends of the primary transcripts and the other representing the whole transcriptome. Both libraries were sequenced and analyzed to identify the conserved sequences of ribosome biding sites and translation start motifs, and to elucidate operon structures present in the transcriptome of P. riograndensis. Sequence analysis of the library enriched in 5′-ends of the primary transcripts was used to identify 1082 transcription start sites (TSS) belonging to novel transcripts and allowed us to determine a promoter consensus sequence and regulatory sequences in 5′ untranslated regions including riboswitches. A putative thiamine pyrophosphate dependent riboswitch upstream of the thiamine biosynthesis gene thiC was characterized by translational fusion to a fluorescent reporter gene and shown to function in P. riograndensis SBR5. Conclusions Our RNA-seq analysis provides insight into the P. riograndensis SBR5 transcriptome at the systems level and will be a valuable basis for differential RNA-seq analysis of this bacterium.
Stichworte
Paenibacillus riograndensis; RNA sequencing; Transcriptional start sites; Promoter motifs; Ribosome biding sites; Operon structures; Thiamine pyrophosphate riboswitch Paenibacillus sonchi
Erscheinungsjahr
2017
Zeitschriftentitel
BMC Genomics
Band
18
Art.-Nr.
846
eISSN
1471-2164
Finanzierungs-Informationen
Article Processing Charge funded by the Deutsche Forschungsgemeinschaft and the Open Access Publication Fund of Bielefeld University.
Page URI
https://pub.uni-bielefeld.de/record/2914662

Zitieren

Fernandes de Brito L, Irla M, Kalinowski J, Wendisch VF. Detailed transcriptome analysis of the plant growth promoting Paenibacillus riograndensis SBR5 by using RNA-seq technology. BMC Genomics. 2017;18: 846.
Fernandes de Brito, L., Irla, M., Kalinowski, J., & Wendisch, V. F. (2017). Detailed transcriptome analysis of the plant growth promoting Paenibacillus riograndensis SBR5 by using RNA-seq technology. BMC Genomics, 18, 846. doi:10.1186/s12864-017-4235-z
Fernandes de Brito, L., Irla, M., Kalinowski, J., and Wendisch, V. F. (2017). Detailed transcriptome analysis of the plant growth promoting Paenibacillus riograndensis SBR5 by using RNA-seq technology. BMC Genomics 18:846.
Fernandes de Brito, L., et al., 2017. Detailed transcriptome analysis of the plant growth promoting Paenibacillus riograndensis SBR5 by using RNA-seq technology. BMC Genomics, 18: 846.
L. Fernandes de Brito, et al., “Detailed transcriptome analysis of the plant growth promoting Paenibacillus riograndensis SBR5 by using RNA-seq technology”, BMC Genomics, vol. 18, 2017, : 846.
Fernandes de Brito, L., Irla, M., Kalinowski, J., Wendisch, V.F.: Detailed transcriptome analysis of the plant growth promoting Paenibacillus riograndensis SBR5 by using RNA-seq technology. BMC Genomics. 18, : 846 (2017).
Fernandes de Brito, Luciana, Irla, Marta, Kalinowski, Jörn, and Wendisch, Volker F. “Detailed transcriptome analysis of the plant growth promoting Paenibacillus riograndensis SBR5 by using RNA-seq technology”. BMC Genomics 18 (2017): 846.
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