Membrane dynamics of resting and internalin B-bound MET receptor tyrosine kinase studied by single-molecule tracking

Harwardt M-LIE, Young P, Bleymüller W, Meyer T, Karathanasis C, Niemann H, Heilemann M, Dietz MS (2017)
FEBS Open Bio 7(9): 1422-1440.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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DOI
Autor*in
Harwardt, Maria-Lena I.E.; Young, Phoebe; Bleymüller, WillemUniBi; Meyer, TimoUniBi; Karathanasis, Christos; Niemann, HartmutUniBi ; Heilemann, Mike; Dietz, Marina S.
Einrichtung
Fakultät für Chemie > Biochemie IV
Abstract / Bemerkung
The human MET receptor tyrosine kinase contributes to vertebrate development and cell proliferation. As a proto-oncogene, it is a target in cancer therapies. MET is also relevant for bacterial infection by Listeria monocytogenes and is activated by the bacterial protein internalin B. The processes of ligand binding, receptor activation, and the diffusion behavior of MET within the plasma membrane as well as its interconnections with various cell components are not fully understood. We investigated the receptor diffusion dynamics using single-particle tracking and imaging fluorescence correlation spectroscopy and elucidated mobility states of resting and internalin B-bound MET. We show that internalin B-bound MET exhibits lower diffusion coefficients and diffuses in a more confined area in the membrane. We report that the fraction of immobile receptors is larger for internalin B-bound receptors than for resting MET. Results of single-particle tracking in cells treated with various cytotoxins depleting cholesterol from the membrane and disrupting the actin cytoskeleton and microtubules suggest that cholesterol and actin influence MET diffusion dynamics, while microtubules do not have any effect.
Erscheinungsjahr
2017
Zeitschriftentitel
FEBS Open Bio
Band
7
Ausgabe
9
Seite(n)
1422-1440
Urheberrecht / Lizenzen
Creative Commons Namensnennung 4.0 International Public License (CC-BY 4.0)
ISSN
2211-5463
eISSN
2211-5463
Page URI
https://pub.uni-bielefeld.de/record/2914444

Zitieren

Harwardt M-LIE, Young P, Bleymüller W, et al. Membrane dynamics of resting and internalin B-bound MET receptor tyrosine kinase studied by single-molecule tracking. FEBS Open Bio. 2017;7(9):1422-1440.
Harwardt, M. - L. I. E., Young, P., Bleymüller, W., Meyer, T., Karathanasis, C., Niemann, H., Heilemann, M., et al. (2017). Membrane dynamics of resting and internalin B-bound MET receptor tyrosine kinase studied by single-molecule tracking. FEBS Open Bio, 7(9), 1422-1440. doi:10.1002/2211-5463.12285
Harwardt, Maria-Lena I.E., Young, Phoebe, Bleymüller, Willem, Meyer, Timo, Karathanasis, Christos, Niemann, Hartmut, Heilemann, Mike, and Dietz, Marina S. 2017. “Membrane dynamics of resting and internalin B-bound MET receptor tyrosine kinase studied by single-molecule tracking”. FEBS Open Bio 7 (9): 1422-1440.
Harwardt, M. - L. I. E., Young, P., Bleymüller, W., Meyer, T., Karathanasis, C., Niemann, H., Heilemann, M., and Dietz, M. S. (2017). Membrane dynamics of resting and internalin B-bound MET receptor tyrosine kinase studied by single-molecule tracking. FEBS Open Bio 7, 1422-1440.
Harwardt, M.-L.I.E., et al., 2017. Membrane dynamics of resting and internalin B-bound MET receptor tyrosine kinase studied by single-molecule tracking. FEBS Open Bio, 7(9), p 1422-1440.
M.-L.I.E. Harwardt, et al., “Membrane dynamics of resting and internalin B-bound MET receptor tyrosine kinase studied by single-molecule tracking”, FEBS Open Bio, vol. 7, 2017, pp. 1422-1440.
Harwardt, M.-L.I.E., Young, P., Bleymüller, W., Meyer, T., Karathanasis, C., Niemann, H., Heilemann, M., Dietz, M.S.: Membrane dynamics of resting and internalin B-bound MET receptor tyrosine kinase studied by single-molecule tracking. FEBS Open Bio. 7, 1422-1440 (2017).
Harwardt, Maria-Lena I.E., Young, Phoebe, Bleymüller, Willem, Meyer, Timo, Karathanasis, Christos, Niemann, Hartmut, Heilemann, Mike, and Dietz, Marina S. “Membrane dynamics of resting and internalin B-bound MET receptor tyrosine kinase studied by single-molecule tracking”. FEBS Open Bio 7.9 (2017): 1422-1440.

1 Zitation in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

SPT and Imaging FCS Provide Complementary Information on the Dynamics of Plasma Membrane Molecules.
Harwardt MIE, Dietz MS, Heilemann M, Wohland T., Biophys J 114(10), 2018
PMID: 29650369

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