Synthesis of Chiral Cyanohydrins by Recombinant Escherichia coli Cells in a Micro-Aqueous Reaction System
Scholz KE, Okrob D, Kopka B, Grünberger A, Pohl M, Jaeger K-E, Krauss U (2012)
Applied and Environmental Microbiology 78(14): 5025-5027.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
Download
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
Autor*in
Scholz, Kathrin Emmi;
Okrob, Daniel;
Kopka, Benita;
Grünberger, AlexanderUniBi;
Pohl, Martina;
Jaeger, Karl-Erich;
Krauss, Ulrich
Abstract / Bemerkung
Synthesis of chiral cyanohydrins is performed in a monophasic micro-aqueous reaction system using whole recombinant Escherichia coli cells expressing the Arabidopsis thaliana hydroxynitrile lyase (AtHNL). Microscopy studies employing a fusion of AtHNL with a flavin-based fluorescent protein (FbFP) reveal that the cells remain intact in the reaction system.
Erscheinungsjahr
2012
Zeitschriftentitel
Applied and Environmental Microbiology
Band
78
Ausgabe
14
Seite(n)
5025-5027
ISSN
0099-2240
eISSN
0099-2240
Page URI
https://pub.uni-bielefeld.de/record/2912747
Zitieren
Scholz KE, Okrob D, Kopka B, et al. Synthesis of Chiral Cyanohydrins by Recombinant Escherichia coli Cells in a Micro-Aqueous Reaction System. Applied and Environmental Microbiology. 2012;78(14):5025-5027.
Scholz, K. E., Okrob, D., Kopka, B., Grünberger, A., Pohl, M., Jaeger, K. - E., & Krauss, U. (2012). Synthesis of Chiral Cyanohydrins by Recombinant Escherichia coli Cells in a Micro-Aqueous Reaction System. Applied and Environmental Microbiology, 78(14), 5025-5027. doi:10.1128/aem.00582-12
Scholz, Kathrin Emmi, Okrob, Daniel, Kopka, Benita, Grünberger, Alexander, Pohl, Martina, Jaeger, Karl-Erich, and Krauss, Ulrich. 2012. “Synthesis of Chiral Cyanohydrins by Recombinant Escherichia coli Cells in a Micro-Aqueous Reaction System”. Applied and Environmental Microbiology 78 (14): 5025-5027.
Scholz, K. E., Okrob, D., Kopka, B., Grünberger, A., Pohl, M., Jaeger, K. - E., and Krauss, U. (2012). Synthesis of Chiral Cyanohydrins by Recombinant Escherichia coli Cells in a Micro-Aqueous Reaction System. Applied and Environmental Microbiology 78, 5025-5027.
Scholz, K.E., et al., 2012. Synthesis of Chiral Cyanohydrins by Recombinant Escherichia coli Cells in a Micro-Aqueous Reaction System. Applied and Environmental Microbiology, 78(14), p 5025-5027.
K.E. Scholz, et al., “Synthesis of Chiral Cyanohydrins by Recombinant Escherichia coli Cells in a Micro-Aqueous Reaction System”, Applied and Environmental Microbiology, vol. 78, 2012, pp. 5025-5027.
Scholz, K.E., Okrob, D., Kopka, B., Grünberger, A., Pohl, M., Jaeger, K.-E., Krauss, U.: Synthesis of Chiral Cyanohydrins by Recombinant Escherichia coli Cells in a Micro-Aqueous Reaction System. Applied and Environmental Microbiology. 78, 5025-5027 (2012).
Scholz, Kathrin Emmi, Okrob, Daniel, Kopka, Benita, Grünberger, Alexander, Pohl, Martina, Jaeger, Karl-Erich, and Krauss, Ulrich. “Synthesis of Chiral Cyanohydrins by Recombinant Escherichia coli Cells in a Micro-Aqueous Reaction System”. Applied and Environmental Microbiology 78.14 (2012): 5025-5027.
Daten bereitgestellt von European Bioinformatics Institute (EBI)
10 Zitationen in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
Nitrile Metabolizing Enzymes in Biocatalysis and Biotransformation.
Bhalla TC, Kumar V, Kumar V, Thakur N, Savitri., Appl Biochem Biotechnol 185(4), 2018
PMID: 29380295
Bhalla TC, Kumar V, Kumar V, Thakur N, Savitri., Appl Biochem Biotechnol 185(4), 2018
PMID: 29380295
Hydroxynitrile lyases from cyanogenic millipedes: molecular cloning, heterologous expression, and whole-cell biocatalysis for the production of (R)-mandelonitrile.
Yamaguchi T, Nuylert A, Ina A, Tanabe T, Asano Y., Sci Rep 8(1), 2018
PMID: 29445093
Yamaguchi T, Nuylert A, Ina A, Tanabe T, Asano Y., Sci Rep 8(1), 2018
PMID: 29445093
Identical active sites in hydroxynitrile lyases show opposite enantioselectivity and reveal possible ancestral mechanism.
Jones BJ, Bata Z, Kazlauskas RJ., ACS Catal 7(6), 2017
PMID: 28798888
Jones BJ, Bata Z, Kazlauskas RJ., ACS Catal 7(6), 2017
PMID: 28798888
Recent advances in whole cell biocatalysis techniques bridging from investigative to industrial scale.
Wachtmeister J, Rother D., Curr Opin Biotechnol 42(), 2016
PMID: 27318259
Wachtmeister J, Rother D., Curr Opin Biotechnol 42(), 2016
PMID: 27318259
Microbial engineering for aldehyde synthesis.
Kunjapur AM, Prather KL., Appl Environ Microbiol 81(6), 2015
PMID: 25576610
Kunjapur AM, Prather KL., Appl Environ Microbiol 81(6), 2015
PMID: 25576610
Purification and simultaneous immobilization of Arabidopsis thaliana hydroxynitrile lyase using a family 2 carbohydrate-binding module.
Kopka B, Diener M, Wirtz A, Pohl M, Jaeger KE, Krauss U., Biotechnol J 10(5), 2015
PMID: 25755120
Kopka B, Diener M, Wirtz A, Pohl M, Jaeger KE, Krauss U., Biotechnol J 10(5), 2015
PMID: 25755120
The photophysics of LOV-based fluorescent proteins--new tools for cell biology.
Wingen M, Potzkei J, Endres S, Casini G, Rupprecht C, Fahlke C, Krauss U, Jaeger KE, Drepper T, Gensch T., Photochem Photobiol Sci 13(6), 2014
PMID: 24500379
Wingen M, Potzkei J, Endres S, Casini G, Rupprecht C, Fahlke C, Krauss U, Jaeger KE, Drepper T, Gensch T., Photochem Photobiol Sci 13(6), 2014
PMID: 24500379
Fusion of a flavin-based fluorescent protein to hydroxynitrile lyase from Arabidopsis thaliana improves enzyme stability.
Scholz KE, Kopka B, Wirtz A, Pohl M, Jaeger KE, Krauss U., Appl Environ Microbiol 79(15), 2013
PMID: 23728815
Scholz KE, Kopka B, Wirtz A, Pohl M, Jaeger KE, Krauss U., Appl Environ Microbiol 79(15), 2013
PMID: 23728815
Advanced in vivo applications of blue light photoreceptors as alternative fluorescent proteins.
Drepper T, Gensch T, Pohl M., Photochem Photobiol Sci 12(7), 2013
PMID: 23660639
Drepper T, Gensch T, Pohl M., Photochem Photobiol Sci 12(7), 2013
PMID: 23660639
19 References
Daten bereitgestellt von Europe PubMed Central.
An R-selective hydroxynitrile lyase from Arabidopsis thaliana with an alpha/beta-hydrolase fold.
Andexer J, von Langermann J, Mell A, Bocola M, Kragl U, Eggert T, Pohl M., Angew. Chem. Int. Ed. Engl. 46(45), 2007
PMID: 17907254
Andexer J, von Langermann J, Mell A, Bocola M, Kragl U, Eggert T, Pohl M., Angew. Chem. Int. Ed. Engl. 46(45), 2007
PMID: 17907254
The enzymatic transformation of water-insoluble reactants in nonaqueous solvents. Conversion of cholesterol to cholest-4-ene-3-one by a sp
Buckland BC, Dunnill P, Lilly MD., 1975
Buckland BC, Dunnill P, Lilly MD., 1975
Substrate specificity of mutants of the hydroxynitrile lyase from Manihot esculenta.
Buhler H, Effenberger F, Forster S, Roos J, Wajant H., Chembiochem 4(2-3), 2003
PMID: 12616635
Buhler H, Effenberger F, Forster S, Roos J, Wajant H., Chembiochem 4(2-3), 2003
PMID: 12616635
The photoreversible fluorescent protein iLOV outperforms GFP as a reporter of plant virus infection.
Chapman S, Faulkner C, Kaiserli E, Garcia-Mata C, Savenkov EI, Roberts AG, Oparka KJ, Christie JM., Proc. Natl. Acad. Sci. U.S.A. 105(50), 2008
PMID: 19060199
Chapman S, Faulkner C, Kaiserli E, Garcia-Mata C, Savenkov EI, Roberts AG, Oparka KJ, Christie JM., Proc. Natl. Acad. Sci. U.S.A. 105(50), 2008
PMID: 19060199
Hydroxynitrile lyases: insights into biochemistry, discovery, and engineering
Dadashipour M, Asano Y., 2011
Dadashipour M, Asano Y., 2011
Enzymatic reduction of ketones in "micro-aqueous" media catalyzed by ADH-A from Rhodococcus ruber.
de Gonzalo G, Lavandera I, Faber K, Kroutil W., Org. Lett. 9(11), 2007
PMID: 17469836
de Gonzalo G, Lavandera I, Faber K, Kroutil W., Org. Lett. 9(11), 2007
PMID: 17469836
Reporter proteins for in vivo fluorescence without oxygen.
Drepper T, Eggert T, Circolone F, Heck A, Krauss U, Guterl JK, Wendorff M, Losi A, Gartner W, Jaeger KE., Nat. Biotechnol. 25(4), 2007
PMID: 17351616
Drepper T, Eggert T, Circolone F, Heck A, Krauss U, Guterl JK, Wendorff M, Losi A, Gartner W, Jaeger KE., Nat. Biotechnol. 25(4), 2007
PMID: 17351616
Flavin mononucleotide-based fluorescent reporter proteins outperform green fluorescent protein-like proteins as quantitative in vivo real-time reporters.
Drepper T, Huber R, Heck A, Circolone F, Hillmer AK, Buchs J, Jaeger KE., Appl. Environ. Microbiol. 76(17), 2010
PMID: 20601504
Drepper T, Huber R, Heck A, Circolone F, Hillmer AK, Buchs J, Jaeger KE., Appl. Environ. Microbiol. 76(17), 2010
PMID: 20601504
Uneven twins: comparison of two enantiocomplementary hydroxynitrile lyases with alpha/beta-hydrolase fold.
Guterl JK, Andexer JN, Sehl T, von Langermann J, Frindi-Wosch I, Rosenkranz T, Fitter J, Gruber K, Kragl U, Eggert T, Pohl M., J. Biotechnol. 141(3-4), 2009
PMID: 19433222
Guterl JK, Andexer JN, Sehl T, von Langermann J, Frindi-Wosch I, Rosenkranz T, Fitter J, Gruber K, Kragl U, Eggert T, Pohl M., J. Biotechnol. 141(3-4), 2009
PMID: 19433222
Industrial biotechnology for the production of bio-based chemicals--a cradle-to-grave perspective.
Hatti-Kaul R, Tornvall U, Gustafsson L, Borjesson P., Trends Biotechnol. 25(3), 2007
PMID: 17234288
Hatti-Kaul R, Tornvall U, Gustafsson L, Borjesson P., Trends Biotechnol. 25(3), 2007
PMID: 17234288
Asymmetric reduction of ketones with recombinant E. coli whole cells in neat substrates.
Jakoblinnert A, Mladenov R, Paul A, Sibilla F, Schwaneberg U, Ansorge-Schumacher MB, de Maria PD., Chem. Commun. (Camb.) 47(44), 2011
PMID: 22005469
Jakoblinnert A, Mladenov R, Paul A, Sibilla F, Schwaneberg U, Ansorge-Schumacher MB, de Maria PD., Chem. Commun. (Camb.) 47(44), 2011
PMID: 22005469
Improving enzymes by using them in organic solvents.
Klibanov AM., Nature 409(6817), 2001
PMID: 11196652
Klibanov AM., Nature 409(6817), 2001
PMID: 11196652
Whole cell biocatalysis in nonconventional media.
Nikolova P, Ward OP., J. Ind. Microbiol. 12(2), 1993
PMID: 7764158
Nikolova P, Ward OP., J. Ind. Microbiol. 12(2), 1993
PMID: 7764158
Hydroxynitrile lyase from : identification of reaction parameters for enantiopure cyanohydrin synthesis by pure and immobilized catalyst
Okrob D., 2011
Okrob D., 2011
Reductions of keto esters with baker's yeast in organic solvents—a comparison with the results in water
Rotthaus O, Krüger D, Demuth M, Schaffner K., 1997
Rotthaus O, Krüger D, Demuth M, Schaffner K., 1997
Industrial potential of organic solvent tolerant bacteria.
Sardessai YN, Bhosle S., Biotechnol. Prog. 20(3), 2004
PMID: 15176865
Sardessai YN, Bhosle S., Biotechnol. Prog. 20(3), 2004
PMID: 15176865
A genetically encoded tag for correlated light and electron microscopy of intact cells, tissues, and organisms.
Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y, Ellisman MH, Tsien RY., PLoS Biol. 9(4), 2011
PMID: 21483721
Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y, Ellisman MH, Tsien RY., PLoS Biol. 9(4), 2011
PMID: 21483721
Flavin mononucleotide-based fluorescent protein as an oxygen-independent reporter in Candida albicans and Saccharomyces cerevisiae.
Tielker D, Eichhof I, Jaeger KE, Ernst JF., Eukaryotic Cell 8(6), 2009
PMID: 19377038
Tielker D, Eichhof I, Jaeger KE, Ernst JF., Eukaryotic Cell 8(6), 2009
PMID: 19377038
Utilization of hydrophobic bacterium Rhodococcus opacus B-4 as whole-cell catalyst in anhydrous organic solvents.
Yamashita S, Satoi M, Iwasa Y, Honda K, Sameshima Y, Omasa T, Kato J, Ohtake H., Appl. Microbiol. Biotechnol. 74(4), 2006
PMID: 17123076
Yamashita S, Satoi M, Iwasa Y, Honda K, Sameshima Y, Omasa T, Kato J, Ohtake H., Appl. Microbiol. Biotechnol. 74(4), 2006
PMID: 17123076
Export
Markieren/ Markierung löschen
Markierte Publikationen
Web of Science
Dieser Datensatz im Web of Science®Quellen
PMID: 22544258
PubMed | Europe PMC
Suchen in