The development and application of a single-cell biosensor for the detection of l-methionine and branched-chain amino acids

Mustafi N, Grünberger A, Kohlheyer D, Bott M, Frunzke J (2012)
Metabolic Engineering 14(4): 449-457.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
; ; ; ;
Abstract / Bemerkung
The detection and quantification of specific metabolites in single bacterial cells is a major goal for industrial biotechnology. We have developed a biosensor based on the transcriptional regulator Lrp that detects intracellular l-methionine and branched-chain amino acids in Corynebacterium glutamicum. In assays, fluorescence output showed a linear relationship with cytoplasmic concentrations of the effector amino acids. In increasing order, the affinity of Lrp for the amino acids is l-valine, l-isoleucine, l-leucine and l-methionine. The sensor was applied for online monitoring and analysis of cell-to-cell variability of l-valine production by the pyruvate dehydrogenase-deficient C. glutamicum strain ΔaceE. Finally, the sensor system was successfully used in a high-throughput (HT) FACS screen for the isolation of amino acid-producing mutants after random mutagenesis of a non-producing wild type strain. These applications illustrate how one of nature's sensor devices – transcriptional regulators – can be used for the analysis, directed evolution and HT screening for microbial strain development. Highlights ► Development of a single cell biosensor for the intracellular detection of amino acids. ► Biosensor-based online monitoring of valine production of a C. glutamicum strain. ► Visualization of cell-to-cell variability of microbial production strains. ► Establishment of a FACS high-throughput screening.
Erscheinungsjahr
Zeitschriftentitel
Metabolic Engineering
Band
14
Ausgabe
4
Seite(n)
449-457
ISSN
PUB-ID

Zitieren

Mustafi N, Grünberger A, Kohlheyer D, Bott M, Frunzke J. The development and application of a single-cell biosensor for the detection of l-methionine and branched-chain amino acids. Metabolic Engineering. 2012;14(4):449-457.
Mustafi, N., Grünberger, A., Kohlheyer, D., Bott, M., & Frunzke, J. (2012). The development and application of a single-cell biosensor for the detection of l-methionine and branched-chain amino acids. Metabolic Engineering, 14(4), 449-457. doi:10.1016/j.ymben.2012.02.002
Mustafi, N., Grünberger, A., Kohlheyer, D., Bott, M., and Frunzke, J. (2012). The development and application of a single-cell biosensor for the detection of l-methionine and branched-chain amino acids. Metabolic Engineering 14, 449-457.
Mustafi, N., et al., 2012. The development and application of a single-cell biosensor for the detection of l-methionine and branched-chain amino acids. Metabolic Engineering, 14(4), p 449-457.
N. Mustafi, et al., “The development and application of a single-cell biosensor for the detection of l-methionine and branched-chain amino acids”, Metabolic Engineering, vol. 14, 2012, pp. 449-457.
Mustafi, N., Grünberger, A., Kohlheyer, D., Bott, M., Frunzke, J.: The development and application of a single-cell biosensor for the detection of l-methionine and branched-chain amino acids. Metabolic Engineering. 14, 449-457 (2012).
Mustafi, Nurije, Grünberger, Alexander, Kohlheyer, Dietrich, Bott, Michael, and Frunzke, Julia. “The development and application of a single-cell biosensor for the detection of l-methionine and branched-chain amino acids”. Metabolic Engineering 14.4 (2012): 449-457.

70 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Small molecule signaling, regulation, and potential applications in cellular therapeutics.
McNerney MP, Styczynski MP., Wiley Interdiscip Rev Syst Biol Med 10(2), 2018
PMID: 28960879
Engineering tunable biosensors for monitoring putrescine in Escherichia coli.
Chen XF, Xia XX, Lee SY, Qian ZG., Biotechnol Bioeng 115(4), 2018
PMID: 29251347
Synthetic addiction extends the productive life time of engineered Escherichia coli populations.
Rugbjerg P, Sarup-Lytzen K, Nagy M, Sommer MOA., Proc Natl Acad Sci U S A 115(10), 2018
PMID: 29463739
Transcription Factor-Based Biosensors in High-Throughput Screening: Advances and Applications.
Cheng F, Tang XL, Kardashliev T., Biotechnol J 13(7), 2018
PMID: 29485214
Production of L-valine from metabolically engineered Corynebacterium glutamicum.
Wang X, Zhang H, Quinn PJ., Appl Microbiol Biotechnol 102(10), 2018
PMID: 29594358
A Pichia pastoris single-cell biosensor for detection of enzymatically produced methanol.
Takeya T, Yurimoto H, Sakai Y., Appl Microbiol Biotechnol 102(16), 2018
PMID: 29948115
Emerging Opportunities for Synthetic Biology in Agriculture.
Goold HD, Wright P, Hailstones D., Genes (Basel) 9(7), 2018
PMID: 29986428
Engineering of an Lrp family regulator SACE_Lrp improves erythromycin production in Saccharopolyspora erythraea.
Liu J, Chen Y, Wang W, Ren M, Wu P, Wang Y, Li C, Zhang L, Wu H, Weaver DT, Zhang B., Metab Eng 39(), 2017
PMID: 27794466
Whole-cell Escherichia coli lactate biosensor for monitoring mammalian cell cultures during biopharmaceutical production.
Goers L, Ainsworth C, Goey CH, Kontoravdi C, Freemont PS, Polizzi KM., Biotechnol Bioeng 114(6), 2017
PMID: 28112405
Coarse-graining bacteria colonies for modelling critical solute distributions in picolitre bioreactors for bacterial studies on single-cell level.
Westerwalbesloh C, Grünberger A, Wiechert W, Kohlheyer D, von Lieres E., Microb Biotechnol 10(4), 2017
PMID: 28371389
A growth- and bioluminescence-based bioreporter for the in vivo detection of novel biocatalysts.
van Rossum T, Muras A, Baur MJJ, Creutzburg SCA, van der Oost J, Kengen SWM., Microb Biotechnol 10(3), 2017
PMID: 28393499
Systems metabolic engineering strategies for the production of amino acids.
Ma Q, Zhang Q, Xu Q, Zhang C, Li Y, Fan X, Xie X, Chen N., Synth Syst Biotechnol 2(2), 2017
PMID: 29062965
Homogenizing bacterial cell factories: Analysis and engineering of phenotypic heterogeneity.
Binder D, Drepper T, Jaeger KE, Delvigne F, Wiechert W, Kohlheyer D, Grünberger A., Metab Eng 42(), 2017
PMID: 28645641
Assessing glycolytic flux alterations resulting from genetic perturbations in E. coli using a biosensor.
Lehning CE, Siedler S, Ellabaan MMH, Sommer MOA., Metab Eng 42(), 2017
PMID: 28709932
Recombineering using RecET in Corynebacterium glutamicum ATCC14067 via a self-excisable cassette.
Huang Y, Li L, Xie S, Zhao N, Han S, Lin Y, Zheng S., Sci Rep 7(1), 2017
PMID: 28801604
Engineering Microbial Metabolite Dynamics and Heterogeneity.
Schmitz AC, Hartline CJ, Zhang F., Biotechnol J 12(10), 2017
PMID: 28901715
CRISPR-Cas9D10A Nickase-Assisted Genome Editing in Lactobacillus casei.
Song X, Huang H, Xiong Z, Ai L, Yang S., Appl Environ Microbiol 83(22), 2017
PMID: 28864652
Beyond the bulk: disclosing the life of single microbial cells.
Rosenthal K, Oehling V, Dusny C, Schmid A., FEMS Microbiol Rev 41(6), 2017
PMID: 29029257
Transcription factor-based biosensors in biotechnology: current state and future prospects.
Mahr R, Frunzke J., Appl Microbiol Biotechnol 100(1), 2016
PMID: 26521244
Metabolic engineering of Saccharomyces cerevisiae for the overproduction of short branched-chain fatty acids.
Yu AQ, Pratomo Juwono NK, Foo JL, Leong SSJ, Chang MW., Metab Eng 34(), 2016
PMID: 26721212
Genetically encoded sensors enable real-time observation of metabolite production.
Rogers JK, Church GM., Proc Natl Acad Sci U S A 113(9), 2016
PMID: 26858408
Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi).
Cleto S, Jensen JV, Wendisch VF, Lu TK., ACS Synth Biol 5(5), 2016
PMID: 26829286
Foundations and Emerging Paradigms for Computing in Living Cells.
Ma KC, Perli SD, Lu TK., J Mol Biol 428(5 pt b), 2016
PMID: 26908220
Synthetic biology to access and expand nature's chemical diversity.
Smanski MJ, Zhou H, Claesen J, Shen B, Fischbach MA, Voigt CA., Nat Rev Microbiol 14(3), 2016
PMID: 26876034
Biosensor-based engineering of biosynthetic pathways.
Rogers JK, Taylor ND, Church GM., Curr Opin Biotechnol 42(), 2016
PMID: 26998575
Exploiting nongenetic cell-to-cell variation for enhanced biosynthesis.
Xiao Y, Bowen CH, Liu D, Zhang F., Nat Chem Biol 12(5), 2016
PMID: 26999780
Updates on industrial production of amino acids using Corynebacterium glutamicum.
Wendisch VF, Jorge JMP, Pérez-García F, Sgobba E., World J Microbiol Biotechnol 32(6), 2016
PMID: 27116971
Recent advances in amino acid production by microbial cells.
Hirasawa T, Shimizu H., Curr Opin Biotechnol 42(), 2016
PMID: 27151315
Screening of an Escherichia coli promoter library for a phenylalanine biosensor.
Mahr R, von Boeselager RF, Wiechert J, Frunzke J., Appl Microbiol Biotechnol 100(15), 2016
PMID: 27170323
Light-Controlled Cell Factories: Employing Photocaged Isopropyl-β-d-Thiogalactopyranoside for Light-Mediated Optimization of lac Promoter-Based Gene Expression and (+)-Valencene Biosynthesis in Corynebacterium glutamicum.
Binder D, Frohwitter J, Mahr R, Bier C, Grünberger A, Loeschcke A, Peters-Wendisch P, Kohlheyer D, Pietruszka J, Frunzke J, Jaeger KE, Wendisch VF, Drepper T., Appl Environ Microbiol 82(20), 2016
PMID: 27520809
Combinatorial and high-throughput screening approaches for strain engineering.
Liu W, Jiang R., Appl Microbiol Biotechnol 99(5), 2015
PMID: 25634017
A giant market and a powerful metabolism: L-lysine provided by Corynebacterium glutamicum.
Eggeling L, Bott M., Appl Microbiol Biotechnol 99(8), 2015
PMID: 25761623
Technical bias of microcultivation environments on single-cell physiology.
Dusny C, Grünberger A, Probst C, Wiechert W, Kohlheyer D, Schmid A., Lab Chip 15(8), 2015
PMID: 25710324
Anaerobic growth of Corynebacterium glutamicum via mixed-acid fermentation.
Michel A, Koch-Koerfges A, Krumbach K, Brocker M, Bott M., Appl Environ Microbiol 81(21), 2015
PMID: 26276118
Developing a high-throughput screening method for threonine overproduction based on an artificial promoter.
Liu Y, Li Q, Zheng P, Zhang Z, Liu Y, Sun C, Cao G, Zhou W, Wang X, Zhang D, Zhang T, Sun J, Ma Y., Microb Cell Fact 14(), 2015
PMID: 26296345
Biosensor-driven adaptive laboratory evolution of l-valine production in Corynebacterium glutamicum.
Mahr R, Gätgens C, Gätgens J, Polen T, Kalinowski J, Frunzke J., Metab Eng 32(), 2015
PMID: 26453945
Protein design for pathway engineering.
Eriksen DT, Lian J, Zhao H., J Struct Biol 185(2), 2014
PMID: 23558037
Analysis of SOS-induced spontaneous prophage induction in Corynebacterium glutamicum at the single-cell level.
Nanda AM, Heyer A, Krämer C, Grünberger A, Kohlheyer D, Frunzke J., J Bacteriol 196(1), 2014
PMID: 24163339
Novel biosensors based on flavonoid-responsive transcriptional regulators introduced into Escherichia coli.
Siedler S, Stahlhut SG, Malla S, Maury J, Neves AR., Metab Eng 21(), 2014
PMID: 24188962
Pushing product formation to its limit: metabolic engineering of Corynebacterium glutamicum for L-leucine overproduction.
Vogt M, Haas S, Klaffl S, Polen T, Eggeling L, van Ooyen J, Bott M., Metab Eng 22(), 2014
PMID: 24333966
Application of a genetically encoded biosensor for live cell imaging of L-valine production in pyruvate dehydrogenase complex-deficient Corynebacterium glutamicum strains.
Mustafi N, Grünberger A, Mahr R, Helfrich S, Nöh K, Blombach B, Kohlheyer D, Frunzke J., PLoS One 9(1), 2014
PMID: 24465669
Looking for the pick of the bunch: high-throughput screening of producing microorganisms with biosensors.
Schallmey M, Frunzke J, Eggeling L, Marienhagen J., Curr Opin Biotechnol 26(), 2014
PMID: 24480185
Application of metabolic engineering for the biotechnological production of L-valine.
Oldiges M, Eikmanns BJ, Blombach B., Appl Microbiol Biotechnol 98(13), 2014
PMID: 24816722
Escherichia [corrected] coli ribose binding protein based bioreporters revisited.
Reimer A, Yagur-Kroll S, Belkin S, Roy S, van der Meer JR., Sci Rep 4(), 2014
PMID: 25005019
Light-responsive control of bacterial gene expression: precise triggering of the lac promoter activity using photocaged IPTG.
Binder D, Grünberger A, Loeschcke A, Probst C, Bier C, Pietruszka J, Wiechert W, Kohlheyer D, Jaeger KE, Drepper T., Integr Biol (Camb) 6(8), 2014
PMID: 24894989
Methionine production--a critical review.
Willke T., Appl Microbiol Biotechnol 98(24), 2014
PMID: 25381187
Bio-based production of organic acids with Corynebacterium glutamicum.
Wieschalka S, Blombach B, Bott M, Eikmanns BJ., Microb Biotechnol 6(2), 2013
PMID: 23199277
Monitoring of population dynamics of Corynebacterium glutamicum by multiparameter flow cytometry.
Neumeyer A, Hübschmann T, Müller S, Frunzke J., Microb Biotechnol 6(2), 2013
PMID: 23279937
Corynebacterium glutamicum promoters: a practical approach.
Pátek M, Holátko J, Busche T, Kalinowski J, Nešvera J., Microb Biotechnol 6(2), 2013
PMID: 23305350
Recombineering in Corynebacterium glutamicum combined with optical nanosensors: a general strategy for fast producer strain generation.
Binder S, Siedler S, Marienhagen J, Bott M, Eggeling L., Nucleic Acids Res 41(12), 2013
PMID: 23630315
Microfluidic growth chambers with optical tweezers for full spatial single-cell control and analysis of evolving microbes.
Probst C, Grünberger A, Wiechert W, Kohlheyer D., J Microbiol Methods 95(3), 2013
PMID: 24041615
Microfluidic picoliter bioreactor for microbial single-cell analysis: fabrication, system setup, and operation.
Gruenberger A, Probst C, Heyer A, Wiechert W, Frunzke J, Kohlheyer D., J Vis Exp (82), 2013
PMID: 24336165

48 References

Daten bereitgestellt von Europe PubMed Central.

Novel hydroxycarotenoids with improved antioxidative properties produced by gene combination in Escherichia coli.
Albrecht M, Takaichi S, Steiger S, Wang ZY, Sandmann G., Nat. Biotechnol. 18(8), 2000
PMID: 10932152
Isolation and characterization of carotenoid hyperproducing mutants of yeast by flow cytometry and cell sorting.
An GH, Bielich J, Auerbach R, Johnson EA., Biotechnology (N.Y.) 9(1), 1991
PMID: 1367215
Toward a science of metabolic engineering.
Bailey JE., Science 252(5013), 1991
PMID: 2047876
Comparative 13C metabolic flux analysis of pyruvate dehydrogenase complex-deficient, L-valine-producing Corynebacterium glutamicum.
Bartek T, Blombach B, Lang S, Eikmanns BJ, Wiechert W, Oldiges M, Noh K, Noack S., Appl. Environ. Microbiol. 77(18), 2011
PMID: 21784914
Importance of NADPH supply for improved L-valine formation in Corynebacterium glutamicum.
Bartek T, Blombach B, Zonnchen E, Makus P, Lang S, Eikmanns BJ, Oldiges M., Biotechnol. Prog. 26(2), 2010
PMID: 20014412
L-valine production with pyruvate dehydrogenase complex-deficient Corynebacterium glutamicum.
Blombach B, Schreiner ME, Holatko J, Bartek T, Oldiges M, Eikmanns BJ., Appl. Environ. Microbiol. 73(7), 2007
PMID: 17293513
Corynebacterium glutamicum tailored for high-yield L-valine production.
Blombach B, Schreiner ME, Bartek T, Oldiges M, Eikmanns BJ., Appl. Microbiol. Biotechnol. 79(3), 2008
PMID: 18379776
Towards methionine overproduction in Corynebacterium glutamicum--methanethiol and dimethyldisulfide as reduced sulfur sources.
Bolten CJ, Schroder H, Dickschat J, Wittmann C., J. Microbiol. Biotechnol. 20(8), 2010
PMID: 20798582

Burkovski, 2008
Fluorescent proteins as a toolkit for in vivo imaging.
Chudakov DM, Lukyanov S, Lukyanov KA., Trends Biotechnol. 23(12), 2005
PMID: 16269193
Genetically encoded sensors for metabolites.
Deuschle K, Fehr M, Hilpert M, Lager I, Lalonde S, Looger LL, Okumoto S, Persson J, Schmidt A, Frommer WB., Cytometry A 64(1), 2005
PMID: 15688353
High-throughput metabolic engineering: advances in small-molecule screening and selection.
Dietrich JA, McKee AE, Keasling JD., Annu. Rev. Biochem. 79(), 2010
PMID: 20367033
Isoleucine excretion in Corynebacterium glutamicum: evidence for a specific efflux carrier system
Ebbighausen, Appl. Microbiol. Biotechnol. 31(), 1989
Amino acids
Eggeling, 2001

Eggeling, 2005
Stochastic gene expression in a single cell.
Elowitz MB, Levine AJ, Siggia ED, Swain PS., Science 297(5584), 2002
PMID: 12183631
Genetically encoded biosensors based on engineered fluorescent proteins.
Frommer WB, Davidson MW, Campbell RE., Chem Soc Rev 38(10), 2009
PMID: 19771330
Population Heterogeneity in Corynebacterium glutamicum ATCC 13032 caused by prophage CGP3.
Frunzke J, Bramkamp M, Schweitzer JE, Bott M., J. Bacteriol. 190(14), 2008
PMID: 18487330
The fluorescent toolbox for assessing protein location and function.
Giepmans BN, Adams SR, Ellisman MH, Tsien RY., Science 312(5771), 2006
PMID: 16614209
Single cell metabolomics.
Heinemann M, Zenobi R., Curr. Opin. Biotechnol. 22(1), 2010
PMID: 20934866
Amino acid production processes.
Ikeda M., Adv. Biochem. Eng. Biotechnol. 79(), 2003
PMID: 12523387

AUTHOR UNKNOWN, 0
Export of L-isoleucine from Corynebacterium glutamicum: a two-gene-encoded member of a new translocator family.
Kennerknecht N, Sahm H, Yen MR, Patek M, Saier Jr MH Jr, Eggeling L., J. Bacteriol. 184(14), 2002
PMID: 12081967
Studies on the amino acid fermentation: I. Production of l-glutamic acid by various microorganisms
Kinoshita, J. Gen. Appl. Microbiol. 3(), 1957

AUTHOR UNKNOWN, 0
Amino acids—technical production and use
Leuchtenberger, 1996
The role of physiological heterogeneity in microbial population behavior.
Lidstrom ME, Konopka MC., Nat. Chem. Biol. 6(10), 2010
PMID: 20852608

AUTHOR UNKNOWN, 0
l-Isoleucine Production with Corynebacterium glutamicum: Further Flux Increase and Limitation of Export.
Morbach S, Sahm H, Eggeling L., Appl. Environ. Microbiol. 62(12), 1996
PMID: 16535457
Efflux of compatible solutes in Corynebacterium glutamicum mediated by osmoregulated channel activity.
Ruffert S, Lambert C, Peter H, Wendisch VF, Kramer R., Eur. J. Biochem. 247(2), 1997
PMID: 9266699

Sambrook, 2001
Melanin-based high-throughput screen for L-tyrosine production in Escherichia coli.
Santos CN, Stephanopoulos G., Appl. Environ. Microbiol. 74(4), 2007
PMID: 18156325
A guide to choosing fluorescent proteins.
Shaner NC, Steinbach PA, Tsien RY., Nat. Methods 2(12), 2005
PMID: 16299475
Transcriptional regulation of bioluminesence genes from Vibrio fischeri.
Sitnikov DM, Schineller JB, Baldwin TO., Mol. Microbiol. 17(5), 1995
PMID: 8596430
Characterization of methionine export in Corynebacterium glutamicum.
Trotschel C, Deutenberg D, Bathe B, Burkovski A, Kramer R., J. Bacteriol. 187(11), 2005
PMID: 15901702

AUTHOR UNKNOWN, 0
The green fluorescent protein.
Tsien RY., Annu. Rev. Biochem. 67(), 1998
PMID: 9759496
Where microbiology meets microengineering: design and applications of reporter bacteria.
van der Meer JR, Belkin S., Nat. Rev. Microbiol. 8(7), 2010
PMID: 20514043
Rapid flow cytometry--Nile red assessment of PHA cellular content and heterogeneity in cultures of Pseudomonas aeruginosa 47T2 (NCIB 40044) grown in waste frying oil.
Vidal-Mas J, Resina-Pelfort , Haba E, Comas J, Manresa A, Vives-Rego J., Antonie Van Leeuwenhoek 80(1), 2001
PMID: 11761367
Soft lithography
Xia, Angew. Chem. Int. Ed. 37(), 1998
Biosensors and their applications in microbial metabolic engineering.
Zhang F, Keasling J., Trends Microbiol. 19(7), 2011
PMID: 21664818

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 22583745
PubMed | Europe PMC

Suchen in

Google Scholar