A disposable picolitre bioreactor for cultivation and investigation of industrially relevant bacteria on the single cell level

Grünberger A, Paczia N, Probst C, Schendzielorz G, Eggeling L, Noack S, Wiechert W, Kohlheyer D (2012)
Lab on a Chip 12(11): 2060-2068.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
OA 1.04 MB
Grünberger, AlexanderUniBi; Paczia, Nicole; Probst, Christopher; Schendzielorz, Georg; Eggeling, Lothar; Noack, Stephan; Wiechert, Wolfgang; Kohlheyer, Dietrich
Lab on a Chip
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Grünberger A, Paczia N, Probst C, et al. A disposable picolitre bioreactor for cultivation and investigation of industrially relevant bacteria on the single cell level. Lab on a Chip. 2012;12(11):2060-2068.
Grünberger, A., Paczia, N., Probst, C., Schendzielorz, G., Eggeling, L., Noack, S., Wiechert, W., et al. (2012). A disposable picolitre bioreactor for cultivation and investigation of industrially relevant bacteria on the single cell level. Lab on a Chip, 12(11), 2060-2068. doi:10.1039/c2lc40156h
Grünberger, A., Paczia, N., Probst, C., Schendzielorz, G., Eggeling, L., Noack, S., Wiechert, W., and Kohlheyer, D. (2012). A disposable picolitre bioreactor for cultivation and investigation of industrially relevant bacteria on the single cell level. Lab on a Chip 12, 2060-2068.
Grünberger, A., et al., 2012. A disposable picolitre bioreactor for cultivation and investigation of industrially relevant bacteria on the single cell level. Lab on a Chip, 12(11), p 2060-2068.
A. Grünberger, et al., “A disposable picolitre bioreactor for cultivation and investigation of industrially relevant bacteria on the single cell level”, Lab on a Chip, vol. 12, 2012, pp. 2060-2068.
Grünberger, A., Paczia, N., Probst, C., Schendzielorz, G., Eggeling, L., Noack, S., Wiechert, W., Kohlheyer, D.: A disposable picolitre bioreactor for cultivation and investigation of industrially relevant bacteria on the single cell level. Lab on a Chip. 12, 2060-2068 (2012).
Grünberger, Alexander, Paczia, Nicole, Probst, Christopher, Schendzielorz, Georg, Eggeling, Lothar, Noack, Stephan, Wiechert, Wolfgang, and Kohlheyer, Dietrich. “A disposable picolitre bioreactor for cultivation and investigation of industrially relevant bacteria on the single cell level”. Lab on a Chip 12.11 (2012): 2060-2068.
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52 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Importance of Pyruvate Sensing and Transport for the Resuscitation of Viable but Nonculturable Escherichia coli K-12.
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Reproduction of Large-Scale Bioreactor Conditions on Microfluidic Chips.
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Quantitative modelling of nutrient-limited growth of bacterial colonies in microfluidic cultivation.
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Simple and Precise Counting of Viable Bacteria by Resazurin-Amplified Picoarray Detection.
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Natural biocide cocktails: Combinatorial antibiotic effects of prodigiosin and biosurfactants.
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Homogenizing bacterial cell factories: Analysis and engineering of phenotypic heterogeneity.
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Role of growth rate on the orientational alignment of Escherichia coli in a slit.
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Comparative Single-Cell Analysis of Different E. coli Expression Systems during Microfluidic Cultivation.
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Enhanced protein and biochemical production using CRISPRi-based growth switches.
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Measuring gene expression in single bacterial cells: recent advances in methods and micro-devices.
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Technical bias of microcultivation environments on single-cell physiology.
Dusny C, Grünberger A, Probst C, Wiechert W, Kohlheyer D, Schmid A., Lab Chip 15(8), 2015
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Life under extreme energy limitation: a synthesis of laboratory- and field-based investigations.
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Live cell imaging of SOS and prophage dynamics in isogenic bacterial populations.
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Modeling and CFD simulation of nutrient distribution in picoliter bioreactors for bacterial growth studies on single-cell level.
Westerwalbesloh C, Grünberger A, Stute B, Weber S, Wiechert W, Kohlheyer D, von Lieres E., Lab Chip 15(21), 2015
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Spatiotemporal microbial single-cell analysis using a high-throughput microfluidics cultivation platform.
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Beyond growth rate 0.6: What drives Corynebacterium glutamicum to higher growth rates in defined medium.
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Analysis of SOS-induced spontaneous prophage induction in Corynebacterium glutamicum at the single-cell level.
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Application of a genetically encoded biosensor for live cell imaging of L-valine production in pyruvate dehydrogenase complex-deficient Corynebacterium glutamicum strains.
Mustafi N, Grünberger A, Mahr R, Helfrich S, Nöh K, Blombach B, Kohlheyer D, Frunzke J., PLoS One 9(1), 2014
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Looking for the pick of the bunch: high-throughput screening of producing microorganisms with biosensors.
Schallmey M, Frunzke J, Eggeling L, Marienhagen J., Curr Opin Biotechnol 26(), 2014
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Single-cell microfluidics: opportunity for bioprocess development.
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Frontier microfluidic techniques for short and long-term single cell analysis.
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Light-responsive control of bacterial gene expression: precise triggering of the lac promoter activity using photocaged IPTG.
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Measuring bacterial adaptation dynamics at the single-cell level using a microfluidic chemostat and time-lapse fluorescence microscopy.
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Beyond growth rate 0.6: Corynebacterium glutamicum cultivated in highly diluted environments.
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Monitoring of population dynamics of Corynebacterium glutamicum by multiparameter flow cytometry.
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Construction of a prophage-free variant of Corynebacterium glutamicum ATCC 13032 for use as a platform strain for basic research and industrial biotechnology.
Baumgart M, Unthan S, Rückert C, Sivalingam J, Grünberger A, Kalinowski J, Bott M, Noack S, Frunzke J., Appl Environ Microbiol 79(19), 2013
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Microfluidic growth chambers with optical tweezers for full spatial single-cell control and analysis of evolving microbes.
Probst C, Grünberger A, Wiechert W, Kohlheyer D., J Microbiol Methods 95(3), 2013
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Microfluidic picoliter bioreactor for microbial single-cell analysis: fabrication, system setup, and operation.
Gruenberger A, Probst C, Heyer A, Wiechert W, Frunzke J, Kohlheyer D., J Vis Exp (82), 2013
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A high-throughput approach to identify genomic variants of bacterial metabolite producers at the single-cell level.
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