Light-responsive control of bacterial gene Expression: precise triggering of the lac promoter activity using photocaged IPTG

Binder D, Grünberger A, Loeschcke A, Probst C, Bier C, Pietruszka J, Wiechert W, Kohlheyer D, Jaeger K-E, Drepper T (2014)
Integrative biology 6(8): 755-765.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
OA 5.86 MB
DOI
URN
urn:nbn:de:0070-pub-29125948
Autor*in
Binder, Dennis; Grünberger, AlexanderUniBi; Loeschcke, Anita; Probst, Christopher; Bier, Claus; Pietruszka, Jörg; Wiechert, Wolfgang; Kohlheyer, Dietrich; Jaeger, Karl-Erich; Drepper, Thomas
Einrichtung
Technische Fakultät > AG Multiscale Bioengineering
Abstract / Bemerkung
Light can be used to control numerous cellular processes including protein function and interaction as well as gene expression in a non-invasive fashion and with unprecedented spatiotemporal resolution. However, for chemical phototriggers tight, gradual, and homogeneous light response has never been attained in living cells. Here, we report on a light-responsive bacterial T7 RNA polymerase expression system based on a photocaged derivative of the inducer molecule isopropyl-β-D-thiogalactopyranoside (IPTG). We have comparatively analyzed different Escherichia coli lac promoter-regulated expression systems in batch and microfluidic single-cell cultivation. The lacY-deficient E. coli strain Tuner(DE3) harboring additional plasmid-born copies of the lacI gene exhibited a sensitive and defined response to increasing IPTG concentrations. Photocaged IPTG served as a synthetic photo-switch to convert the E. coli system into an optogenetic expression module allowing for precise and gradual light-triggering of gene expression as demonstrated at the single cell level. Graphical abstract: Light-responsive control of bacterial gene expression: precise triggering of the lac promoter activity using photocaged IPTG
Erscheinungsjahr
2014
Zeitschriftentitel
Integrative biology
Band
6
Ausgabe
8
Seite(n)
755-765
ISSN
1757-9694
eISSN
1757-9708
Page URI
https://pub.uni-bielefeld.de/record/2912594

Zitieren

Binder D, Grünberger A, Loeschcke A, et al. Light-responsive control of bacterial gene Expression: precise triggering of the lac promoter activity using photocaged IPTG. Integrative biology. 2014;6(8):755-765.
Binder, D., Grünberger, A., Loeschcke, A., Probst, C., Bier, C., Pietruszka, J., Wiechert, W., et al. (2014). Light-responsive control of bacterial gene Expression: precise triggering of the lac promoter activity using photocaged IPTG. Integrative biology, 6(8), 755-765. doi:10.1039/c4ib00027g
Binder, Dennis, Grünberger, Alexander, Loeschcke, Anita, Probst, Christopher, Bier, Claus, Pietruszka, Jörg, Wiechert, Wolfgang, Kohlheyer, Dietrich, Jaeger, Karl-Erich, and Drepper, Thomas. 2014. “Light-responsive control of bacterial gene Expression: precise triggering of the lac promoter activity using photocaged IPTG”. Integrative biology 6 (8): 755-765.
Binder, D., Grünberger, A., Loeschcke, A., Probst, C., Bier, C., Pietruszka, J., Wiechert, W., Kohlheyer, D., Jaeger, K. - E., and Drepper, T. (2014). Light-responsive control of bacterial gene Expression: precise triggering of the lac promoter activity using photocaged IPTG. Integrative biology 6, 755-765.
Binder, D., et al., 2014. Light-responsive control of bacterial gene Expression: precise triggering of the lac promoter activity using photocaged IPTG. Integrative biology, 6(8), p 755-765.
D. Binder, et al., “Light-responsive control of bacterial gene Expression: precise triggering of the lac promoter activity using photocaged IPTG”, Integrative biology, vol. 6, 2014, pp. 755-765.
Binder, D., Grünberger, A., Loeschcke, A., Probst, C., Bier, C., Pietruszka, J., Wiechert, W., Kohlheyer, D., Jaeger, K.-E., Drepper, T.: Light-responsive control of bacterial gene Expression: precise triggering of the lac promoter activity using photocaged IPTG. Integrative biology. 6, 755-765 (2014).
Binder, Dennis, Grünberger, Alexander, Loeschcke, Anita, Probst, Christopher, Bier, Claus, Pietruszka, Jörg, Wiechert, Wolfgang, Kohlheyer, Dietrich, Jaeger, Karl-Erich, and Drepper, Thomas. “Light-responsive control of bacterial gene Expression: precise triggering of the lac promoter activity using photocaged IPTG”. Integrative biology 6.8 (2014): 755-765.
Alle Dateien verfügbar unter der/den folgenden Lizenz(en):
Copyright Statement:
Dieses Objekt ist durch das Urheberrecht und/oder verwandte Schutzrechte geschützt. [...]
Volltext(e)
Name
5.86 MB
Access Level
OA Open Access
Zuletzt Hochgeladen
2019-09-06T09:18:50Z
MD5 Prüfsumme
646cb0f92fec2aed49d442542204da00


20 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Control of Protein Activity and Gene Expression by Cyclofen-OH Uncaging.
Zhang W, Hamouri F, Feng Z, Aujard I, Ducos B, Ye S, Weiss S, Volovitch M, Vriz S, Jullien L, Bensimon D., Chembiochem 19(12), 2018
PMID: 29341391
Natural biocide cocktails: Combinatorial antibiotic effects of prodigiosin and biosurfactants.
Hage-Hülsmann J, Grünberger A, Thies S, Santiago-Schübel B, Klein AS, Pietruszka J, Binder D, Hilgers F, Domröse A, Drepper T, Kohlheyer D, Jaeger KE, Loeschcke A., PLoS One 13(7), 2018
PMID: 30024935
Selective Pressure for Biofilm Formation in Bacillus subtilis: Differential Effect of Mutations in the Master Regulator SinR on Bistability.
Kampf J, Gerwig J, Kruse K, Cleverley R, Dormeyer M, Grünberger A, Kohlheyer D, Commichau FM, Lewis RJ, Lewis RJ, Stülke J., MBio 9(5), 2018
PMID: 30181249
Biotechnological production of aromatic compounds of the extended shikimate pathway from renewable biomass.
Lee JH, Wendisch VF., J Biotechnol 257(), 2017
PMID: 27871872
The application of powerful promoters to enhance gene expression in industrial microorganisms.
Zhou S, Du G, Kang Z, Li J, Chen J, Li H, Zhou J., World J Microbiol Biotechnol 33(2), 2017
PMID: 28044271
Production of amino acids - Genetic and metabolic engineering approaches.
Lee JH, Wendisch VF., Bioresour Technol 245(pt b), 2017
PMID: 28552565
Homogenizing bacterial cell factories: Analysis and engineering of phenotypic heterogeneity.
Binder D, Drepper T, Jaeger KE, Delvigne F, Wiechert W, Kohlheyer D, Grünberger A., Metab Eng 42(), 2017
PMID: 28645641
Advanced Gene Manipulation Methods for Stem Cell Theranostics.
Rathnam C, Chueng SD, Yang L, Lee KB., Theranostics 7(11), 2017
PMID: 28824715
Photocaged Arabinose: A Novel Optogenetic Switch for Rapid and Gradual Control of Microbial Gene Expression.
Binder D, Bier C, Grünberger A, Drobietz D, Hage-Hülsmann J, Wandrey G, Büchs J, Kohlheyer D, Loeschcke A, Wiechert W, Jaeger KE, Pietruszka J, Drepper T., Chembiochem 17(4), 2016
PMID: 26677142
Microfluidics and microbial engineering.
Kou S, Cheng D, Sun F, Hsing IM., Lab Chip 16(3), 2016
PMID: 26758660
Light-induced gene expression with photocaged IPTG for induction profiling in a high-throughput screening system.
Wandrey G, Bier C, Binder D, Hoffmann K, Jaeger KE, Pietruszka J, Drepper T, Büchs J., Microb Cell Fact 15(), 2016
PMID: 27107964
A Novel and Fast Purification Method for Nucleoside Transporters.
Hao Z, Thomsen M, Postis VL, Lesiuk A, Sharples D, Wang Y, Bartlam M, Goldman A., Front Mol Biosci 3(), 2016
PMID: 27376071
Comparative Single-Cell Analysis of Different E. coli Expression Systems during Microfluidic Cultivation.
Binder D, Probst C, Grünberger A, Hilgers F, Loeschcke A, Jaeger KE, Kohlheyer D, Drepper T., PLoS One 11(8), 2016
PMID: 27525986
Time-resolved, single-cell analysis of induced and programmed cell death via non-invasive propidium iodide and counterstain perfusion.
Krämer CE, Wiechert W, Kohlheyer D., Sci Rep 6(), 2016
PMID: 27580964
Light-Controlled Cell Factories: Employing Photocaged Isopropyl-β-d-Thiogalactopyranoside for Light-Mediated Optimization of lac Promoter-Based Gene Expression and (+)-Valencene Biosynthesis in Corynebacterium glutamicum.
Binder D, Frohwitter J, Mahr R, Bier C, Grünberger A, Loeschcke A, Peters-Wendisch P, Kohlheyer D, Pietruszka J, Frunzke J, Jaeger KE, Wendisch VF, Drepper T., Appl Environ Microbiol 82(20), 2016
PMID: 27520809
Microfluidic single-cell analysis links boundary environments and individual microbial phenotypes.
Dusny C, Schmid A., Environ Microbiol 17(6), 2015
PMID: 25330456
Technical bias of microcultivation environments on single-cell physiology.
Dusny C, Grünberger A, Probst C, Wiechert W, Kohlheyer D, Schmid A., Lab Chip 15(8), 2015
PMID: 25710324
Vizardous: interactive analysis of microbial populations with single cell resolution.
Helfrich S, Azzouzi CE, Probst C, Seiffarth J, Grünberger A, Wiechert W, Kohlheyer D, Nöh K., Bioinformatics 31(23), 2015
PMID: 26261223
Modeling and CFD simulation of nutrient distribution in picoliter bioreactors for bacterial growth studies on single-cell level.
Westerwalbesloh C, Grünberger A, Stute B, Weber S, Wiechert W, Kohlheyer D, von Lieres E., Lab Chip 15(21), 2015
PMID: 26345659
Spatiotemporal microbial single-cell analysis using a high-throughput microfluidics cultivation platform.
Grünberger A, Probst C, Helfrich S, Nanda A, Stute B, Wiechert W, von Lieres E, Nöh K, Frunzke J, Kohlheyer D., Cytometry A 87(12), 2015
PMID: 26348020

58 References

Daten bereitgestellt von Europe PubMed Central.

Synthetic biology: understanding biological design from synthetic circuits.
Mukherji S, van Oudenaarden A., Nat. Rev. Genet. 10(12), 2009
PMID: 19898500
Synthetic gene networks: the next wave in biotechnology?
Bhalerao KD., Trends Biotechnol. 27(6), 2009
PMID: 19409633
Exploiting plug-and-play synthetic biology for drug discovery and production in microorganisms.
Medema MH, Breitling R, Bovenberg R, Takano E., Nat. Rev. Microbiol. 9(2), 2010
PMID: 21189477
Synthetic biology and metabolic engineering.
Stephanopoulos G., ACS Synth Biol 1(11), 2012
PMID: 23656228
LOV to BLUF: flavoprotein contributions to the optogenetic toolkit.
Christie JM, Gawthorne J, Young G, Fraser NJ, Roe AJ., Mol Plant 5(3), 2012
PMID: 22431563
Lights on and action! Controlling microbial gene expression by light.
Drepper T, Krauss U, Meyer zu Berstenhorst S, Pietruszka J, Jaeger KE., Appl. Microbiol. Biotechnol. 90(1), 2011
PMID: 21336931
Light-controlled tools.
Brieke C, Rohrbach F, Gottschalk A, Mayer G, Heckel A., Angew. Chem. Int. Ed. Engl. 51(34), 2012
PMID: 22829531
Enlightened enzymes: strategies to create novel photoresponsive proteins.
Krauss U, Drepper T, Jaeger KE., Chemistry 17(9), 2011
PMID: 21305623
Recent advances in the photochemical control of protein function.
Riggsbee CW, Deiters A., Trends Biotechnol. 28(9), 2010
PMID: 20667607
Multichromatic control of gene expression in Escherichia coli.
Tabor JJ, Levskaya A, Voigt CA., J. Mol. Biol. 405(2), 2010
PMID: 21035461
From dusk till dawn: one-plasmid systems for light-regulated gene expression.
Ohlendorf R, Vidavski RR, Eldar A, Moffat K, Moglich A., J. Mol. Biol. 416(4), 2012
PMID: 22245580
Real-time optogenetic control of intracellular protein concentration in microbial cell cultures.
Melendez J, Patel M, Oakes BL, Xu P, Morton P, McClean MN., Integr Biol (Camb) 6(3), 2014
PMID: 24477515
Principles and applications of the photochemical control of cellular processes.
Deiters A., Chembiochem 11(1), 2010
PMID: 19911402
Photochemical control of biological processes.
Young DD, Deiters A., Org. Biomol. Chem. 5(7), 2006
PMID: 17377650
Photochemical activation of protein expression in bacterial cells.
Young DD, Deiters A., Angew. Chem. Int. Ed. Engl. 46(23), 2007
PMID: 17458846
A caged doxycycline analogue for photoactivated gene expression.
Cambridge SB, Geissler D, Keller S, Curten B., Angew. Chem. Int. Ed. Engl. 45(14), 2006
PMID: 16506298
Photocaged t7 RNA polymerase for the light activation of transcription and gene function in pro- and eukaryotic cells.
Chou C, Young DD, Deiters A., Chembiochem 11(7), 2010
PMID: 20301166
Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes.
Studier FW, Moffatt BA., J. Mol. Biol. 189(1), 1986
PMID: 3537305
Overview of bacterial expression systems for heterologous protein production: from molecular and biochemical fundamentals to commercial systems.
Terpe K., Appl. Microbiol. Biotechnol. 72(2), 2006
PMID: 16791589

Samuelson, 2011
Bacteriophage T7 RNA polymerase travels far ahead of ribosomes in vivo.
Iost I, Guillerez J, Dreyfus M., J. Bacteriol. 174(2), 1992
PMID: 1729251
Protein production and purification.
Structural Genomics Consortium; China Structural Genomics Consortium; Northeast Structural Genomics Consortium, Graslund S, Nordlund P, Weigelt J, Hallberg BM, Bray J, Gileadi O, Knapp S, Oppermann U, Arrowsmith C, Hui R, Ming J, dhe-Paganon S, Park HW, Savchenko A, Yee A, Edwards A, Vincentelli R, Cambillau C, Kim R, Kim SH, Rao Z, Shi Y, Terwilliger TC, Kim CY, Hung LW, Waldo GS, Peleg Y, Albeck S, Unger T, Dym O, Prilusky J, Sussman JL, Stevens RC, Lesley SA, Wilson IA, Joachimiak A, Collart F, Dementieva I, Donnelly MI, Eschenfeldt WH, Kim Y, Stols L, Wu R, Zhou M, Burley SK, Emtage JS, Sauder JM, Thompson D, Bain K, Luz J, Gheyi T, Zhang F, Atwell S, Almo SC, Bonanno JB, Fiser A, Swaminathan S, Studier FW, Chance MR, Sali A, Acton TB, Xiao R, Zhao L, Ma LC, Hunt JF, Tong L, Cunningham K, Inouye M, Anderson S, Janjua H, Shastry R, Ho CK, Wang D, Wang H, Jiang M, Montelione GT, Stuart DI, Owens RJ, Daenke S, Schutz A, Heinemann U, Yokoyama S, Bussow K, Gunsalus KC., Nat. Methods 5(2), 2008
PMID: 18235434
Evidencing the role of lactose permease in IPTG uptake by Escherichia coli in fed-batch high cell density cultures.
Fernandez-Castane A, Vine CE, Caminal G, Lopez-Santin J., J. Biotechnol. 157(3), 2011
PMID: 22202176
lac operon induction in Escherichia coli: Systematic comparison of IPTG and TMG induction and influence of the transacetylase LacA.
Marbach A, Bettenbrock K., J. Biotechnol. 157(1), 2011
PMID: 22079752
Single cell analysis of gene expression patterns during carbon starvation in Bacillus subtilis reveals large phenotypic variation.
de Jong IG, Veening JW, Kuipers OP., Environ. Microbiol. 14(12), 2012
PMID: 23033921
Functional single-cell analyses: flow cytometry and cell sorting of microbial populations and communities.
Muller S, Nebe-von-Caron G., FEMS Microbiol. Rev. 34(4), 2010
PMID: 20337722
Living with heterogeneities in bioreactors: understanding the effects of environmental gradients on cells.
Lara AR, Galindo E, Ramirez OT, Palomares LA., Mol. Biotechnol. 34(3), 2006
PMID: 17284782
Beyond growth rate 0.6: What drives Corynebacterium glutamicum to higher growth rates in defined medium.
Unthan S, Grunberger A, van Ooyen J, Gatgens J, Heinrich J, Paczia N, Wiechert W, Kohlheyer D, Noack S., Biotechnol. Bioeng. 111(2), 2013
PMID: 23996851
Non-genetic heterogeneity of cells in development: more than just noise.
Huang S., Development 136(23), 2009
PMID: 19906852
Measuring single-cell gene expression dynamics in bacteria using fluorescence time-lapse microscopy.
Young JW, Locke JC, Altinok A, Rosenfeld N, Bacarian T, Swain PS, Mjolsness E, Elowitz MB., Nat Protoc 7(1), 2011
PMID: 22179594
Microfluidic technologies for studying synthetic circuits.
Lin B, Levchenko A., Curr Opin Chem Biol 16(3-4), 2012
PMID: 22609335
Single-cell microfluidics: opportunity for bioprocess development.
Grunberger A, Wiechert W, Kohlheyer D., Curr. Opin. Biotechnol. 29(), 2014
PMID: 24642389
Picoliter nDEP traps enable time-resolved contactless single bacterial cell analysis in controlled microenvironments.
Fritzsch FS, Rosenthal K, Kampert A, Howitz S, Dusny C, Blank LM, Schmid A., Lab Chip 13(3), 2013
PMID: 23223864
Microfluidic growth chambers with optical tweezers for full spatial single-cell control and analysis of evolving microbes.
Probst C, Grunberger A, Wiechert W, Kohlheyer D., J. Microbiol. Methods 95(3), 2013
PMID: 24041615
Robust growth of Escherichia coli.
Wang P, Robert L, Pelletier J, Dang WL, Taddei F, Wright A, Jun S., Curr. Biol. 20(12), 2010
PMID: 20537537
Taking control over control: use of product sensing in single cells to remove flux control at key enzymes in biosynthesis pathways.
Schendzielorz G, Dippong M, Grunberger A, Kohlheyer D, Yoshida A, Binder S, Nishiyama C, Nishiyama M, Bott M, Eggeling L., ACS Synth Biol 3(1), 2013
PMID: 23829416
Streaming instability in growing cell populations.
Mather W, Mondragon-Palomino O, Danino T, Hasty J, Tsimring LS., Phys. Rev. Lett. 104(20), 2010
PMID: 20867071
High-throughput gene expression analysis at the level of single proteins using a microfluidic turbidostat and automated cell tracking.
Ullman G, Wallden M, Marklund EG, Mahmutovic A, Razinkov I, Elf J., Philos. Trans. R. Soc. Lond., B, Biol. Sci. 368(1611), 2012
PMID: 23267179
A disposable picolitre bioreactor for cultivation and investigation of industrially relevant bacteria on the single cell level.
Grunberger A, Paczia N, Probst C, Schendzielorz G, Eggeling L, Noack S, Wiechert W, Kohlheyer D., Lab Chip 12(11), 2012
PMID: 22511122
The development and application of a single-cell biosensor for the detection of l-methionine and branched-chain amino acids.
Mustafi N, Grunberger A, Kohlheyer D, Bott M, Frunzke J., Metab. Eng. 14(4), 2012
PMID: 22583745
Application of a genetically encoded biosensor for live cell imaging of L-valine production in pyruvate dehydrogenase complex-deficient Corynebacterium glutamicum strains.
Mustafi N, Grunberger A, Mahr R, Helfrich S, Noh K, Blombach B, Kohlheyer D, Frunzke J., PLoS ONE 9(1), 2014
PMID: 24465669
Pre-dispositions and epigenetic inheritance in the Escherichia coli lactose operon bistable switch.
Robert L, Paul G, Chen Y, Taddei F, Baigl D, Lindner AB., Mol. Syst. Biol. 6(), 2010
PMID: 20393577
Studies on transformation of Escherichia coli with plasmids.
Hanahan D., J. Mol. Biol. 166(4), 1983
PMID: 6345791

Sambrook, 1989
A novel T7 RNA polymerase dependent expression system for high-level protein production in the phototrophic bacterium Rhodobacter capsulatus.
Katzke N, Arvani S, Bergmann R, Circolone F, Markert A, Svensson V, Jaeger KE, Heck A, Drepper T., Protein Expr. Purif. 69(2), 2009
PMID: 19706327

Alexeyev, Biotechniques 18(), 1995

Rosenau, 2004
Real-time determination of intracellular oxygen in bacteria using a genetically encoded FRET-based biosensor.
Potzkei J, Kunze M, Drepper T, Gensch T, Jaeger KE, Buchs J., BMC Biol. 10(), 2012
PMID: 22439625

Gruenberger, J. Visualized Exp. (82), 2013
The lactose repressor system: paradigms for regulation, allosteric behavior and protein folding.
Wilson CJ, Zhan H, Swint-Kruse L, Matthews KS., Cell. Mol. Life Sci. 64(1), 2007
PMID: 17103112
Quantitative approaches to the study of bistability in the lac operon of Escherichia coli.
Santillan M, Mackey MC., J R Soc Interface 5 Suppl 1(), 2008
PMID: 18426771
Enhancement of solubility in Escherichia coli and purification of an aminotransferase from Sphingopyxis sp. MTA144 for deamination of hydrolyzed fumonisin B(1).
Hartinger D, Heinl S, Schwartz HE, Grabherr R, Schatzmayr G, Haltrich D, Moll WD., Microb. Cell Fact. 9(), 2010
PMID: 20718948
Multistability in the lactose utilization network of Escherichia coli.
Ozbudak EM, Thattai M, Lim HN, Shraiman BI, Van Oudenaarden A., Nature 427(6976), 2004
PMID: 14973486
Beyond growth rate 0.6: Corynebacterium glutamicum cultivated in highly diluted environments.
Grunberger A, van Ooyen J, Paczia N, Rohe P, Schiendzielorz G, Eggeling L, Wiechert W, Kohlheyer D, Noack S., Biotechnol. Bioeng. 110(1), 2012
PMID: 22890752
TREX: a universal tool for the transfer and expression of biosynthetic pathways in bacteria.
Loeschcke A, Markert A, Wilhelm S, Wirtz A, Rosenau F, Jaeger KE, Drepper T., ACS Synth Biol 2(1), 2012
PMID: 23656323
Novel broad host range shuttle vectors for expression in Escherichia coli, Bacillus subtilis and Pseudomonas putida.
Troeschel SC, Thies S, Link O, Real CI, Knops K, Wilhelm S, Rosenau F, Jaeger KE., J. Biotechnol. 161(2), 2012
PMID: 22440389
Synthetic biology: engineering Escherichia coli to see light.
Levskaya A, Chevalier AA, Tabor JJ, Simpson ZB, Lavery LA, Levy M, Davidson EA, Scouras A, Ellington AD, Marcotte EM, Voigt CA., Nature 438(7067), 2005
PMID: 16306980
Charting microbial phenotypes in multiplex nanoliter batch bioreactors.
Dai J, Yoon SH, Sim HY, Yang YS, Oh TK, Kim JF, Hong JW., Anal. Chem. 85(12), 2013
PMID: 23581968
Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®
Quellen

PMID: 24894989
PubMed | Europe PMC

Suchen in

Google Scholar