Spatiotemporal microbial single-cell analysis using a high-throughput microfluidics cultivation platform
Grünberger A, Probst C, Helfrich S, Nanda A, Stute B, Wiechert W, von Lieres E, Nöh K, Frunzke J, Kohlheyer D (2015)
Cytometry / A 87(12): 1101–1115.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
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Autor*in
Grünberger, AlexanderUniBi;
Probst, Christopher;
Helfrich, Stefan;
Nanda, Arun;
Stute, Birgit;
Wiechert, Wolfgang;
von Lieres, Eric;
Nöh, Katharina;
Frunzke, Julia;
Kohlheyer, Dietrich
Abstract / Bemerkung
Cell-to-cell heterogeneity typically evolves due to a manifold of biological and environmental factors and special phenotypes are often relevant for the fate of the whole population but challenging to detect during conventional analysis. We demonstrate a microfluidic single-cell cultivation platform that incorporates several hundred growth chambers, in which isogenic bacteria microcolonies growing in cell monolayers are tracked by automated time-lapse microscopy with spatiotemporal resolution. The device was not explicitly developed for a specific organism, but has a very generic configuration suitable for various different microbial organisms. In the present study, we analyzed Corynebacterium glutamicum microcolonies, thereby generating complete lineage trees and detailed single-cell data on division behavior and morphology in order to demonstrate the platform's overall capabilities. Furthermore, the occurrence of spontaneously induced stress in individual C. glutamicum cells was investigated by analyzing strains with genetically encoded reporter systems and optically visualizing SOS response. The experiments revealed spontaneous SOS induction in the absence of any external trigger comparable to results obtained by flow cytometry (FC) analyzing cell samples from conventional shake flask cultivation. Our microfluidic setup delivers detailed single-cell data with spatial and temporal resolution; complementary information to conventional FC results. © 2015 International Society for Advancement of Cytometry
Erscheinungsjahr
2015
Zeitschriftentitel
Cytometry / A
Band
87
Ausgabe
12
Seite(n)
1101–1115
ISSN
1552-4922
eISSN
1552-4922
Page URI
https://pub.uni-bielefeld.de/record/2912550
Zitieren
Grünberger A, Probst C, Helfrich S, et al. Spatiotemporal microbial single-cell analysis using a high-throughput microfluidics cultivation platform. Cytometry / A. 2015;87(12):1101–1115.
Grünberger, A., Probst, C., Helfrich, S., Nanda, A., Stute, B., Wiechert, W., von Lieres, E., et al. (2015). Spatiotemporal microbial single-cell analysis using a high-throughput microfluidics cultivation platform. Cytometry / A, 87(12), 1101–1115. doi:10.1002/cyto.a.22779
Grünberger, Alexander, Probst, Christopher, Helfrich, Stefan, Nanda, Arun, Stute, Birgit, Wiechert, Wolfgang, von Lieres, Eric, Nöh, Katharina, Frunzke, Julia, and Kohlheyer, Dietrich. 2015. “Spatiotemporal microbial single-cell analysis using a high-throughput microfluidics cultivation platform”. Cytometry / A 87 (12): 1101–1115.
Grünberger, A., Probst, C., Helfrich, S., Nanda, A., Stute, B., Wiechert, W., von Lieres, E., Nöh, K., Frunzke, J., and Kohlheyer, D. (2015). Spatiotemporal microbial single-cell analysis using a high-throughput microfluidics cultivation platform. Cytometry / A 87, 1101–1115.
Grünberger, A., et al., 2015. Spatiotemporal microbial single-cell analysis using a high-throughput microfluidics cultivation platform. Cytometry / A, 87(12), p 1101–1115.
A. Grünberger, et al., “Spatiotemporal microbial single-cell analysis using a high-throughput microfluidics cultivation platform”, Cytometry / A, vol. 87, 2015, pp. 1101–1115.
Grünberger, A., Probst, C., Helfrich, S., Nanda, A., Stute, B., Wiechert, W., von Lieres, E., Nöh, K., Frunzke, J., Kohlheyer, D.: Spatiotemporal microbial single-cell analysis using a high-throughput microfluidics cultivation platform. Cytometry / A. 87, 1101–1115 (2015).
Grünberger, Alexander, Probst, Christopher, Helfrich, Stefan, Nanda, Arun, Stute, Birgit, Wiechert, Wolfgang, von Lieres, Eric, Nöh, Katharina, Frunzke, Julia, and Kohlheyer, Dietrich. “Spatiotemporal microbial single-cell analysis using a high-throughput microfluidics cultivation platform”. Cytometry / A 87.12 (2015): 1101–1115.
Daten bereitgestellt von European Bioinformatics Institute (EBI)
22 Zitationen in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
Importance of Pyruvate Sensing and Transport for the Resuscitation of Viable but Nonculturable Escherichia coli K-12.
Vilhena C, Kaganovitch E, Grünberger A, Motz M, Forné I, Kohlheyer D, Jung K., J Bacteriol 201(3), 2019
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Vilhena C, Kaganovitch E, Grünberger A, Motz M, Forné I, Kohlheyer D, Jung K., J Bacteriol 201(3), 2019
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Growth-dependent recombinant product formation kinetics can be reproduced through engineering of glucose transport and is prone to phenotypic heterogeneity.
Fragoso-Jiménez JC, Baert J, Nguyen TM, Liu W, Sassi H, Goormaghtigh F, Van Melderen L, Gaytán P, Hernández-Chávez G, Martinez A, Delvigne F, Gosset G., Microb Cell Fact 18(1), 2019
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A microfluidic photobioreactor for simultaneous observation and cultivation of single microalgal cells or cell aggregates.
Westerwalbesloh C, Brehl C, Weber S, Probst C, Widzgowski J, Grünberger A, Pfaff C, Nedbal L, Kohlheyer D., PLoS One 14(4), 2019
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Westerwalbesloh C, Brehl C, Weber S, Probst C, Widzgowski J, Grünberger A, Pfaff C, Nedbal L, Kohlheyer D., PLoS One 14(4), 2019
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Laboratory-scale photobiotechnology-current trends and future perspectives.
Morschett H, Loomba V, Huber G, Wiechert W, von Lieres E, Oldiges M., FEMS Microbiol Lett 365(1), 2018
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Morschett H, Loomba V, Huber G, Wiechert W, von Lieres E, Oldiges M., FEMS Microbiol Lett 365(1), 2018
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Quantitative modelling of nutrient-limited growth of bacterial colonies in microfluidic cultivation.
Hornung R, Grünberger A, Westerwalbesloh C, Kohlheyer D, Gompper G, Elgeti J., J R Soc Interface 15(139), 2018
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Hornung R, Grünberger A, Westerwalbesloh C, Kohlheyer D, Gompper G, Elgeti J., J R Soc Interface 15(139), 2018
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A Novel Methodology for Characterizing Cell Subpopulations in Automated Time-lapse Microscopy.
Hattab G, Wiesmann V, Becker A, Munzner T, Nattkemper TW., Front Bioeng Biotechnol 6(), 2018
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Catalytically active inclusion bodies of L-lysine decarboxylase from E. coli for 1,5-diaminopentane production.
Kloss R, Limberg MH, Mackfeld U, Hahn D, Grünberger A, Jäger VD, Krauss U, Oldiges M, Pohl M., Sci Rep 8(1), 2018
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Simple and Precise Counting of Viable Bacteria by Resazurin-Amplified Picoarray Detection.
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Natural biocide cocktails: Combinatorial antibiotic effects of prodigiosin and biosurfactants.
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Kampf J, Gerwig J, Kruse K, Cleverley R, Dormeyer M, Grünberger A, Kohlheyer D, Commichau FM, Lewis RJ, Lewis RJ, Stülke J., MBio 9(5), 2018
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Germination and Growth Analysis of Streptomyces lividans at the Single-Cell Level Under Varying Medium Compositions.
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Koepff J, Sachs CC, Wiechert W, Kohlheyer D, Nöh K, Oldiges M, Grünberger A., Front Microbiol 9(), 2018
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Coarse-graining bacteria colonies for modelling critical solute distributions in picolitre bioreactors for bacterial studies on single-cell level.
Westerwalbesloh C, Grünberger A, Wiechert W, Kohlheyer D, von Lieres E., Microb Biotechnol 10(4), 2017
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Westerwalbesloh C, Grünberger A, Wiechert W, Kohlheyer D, von Lieres E., Microb Biotechnol 10(4), 2017
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Taking control over microbial populations: Current approaches for exploiting biological noise in bioprocesses.
Delvigne F, Baert J, Sassi H, Fickers P, Grünberger A, Dusny C., Biotechnol J 12(7), 2017
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Delvigne F, Baert J, Sassi H, Fickers P, Grünberger A, Dusny C., Biotechnol J 12(7), 2017
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Homogenizing bacterial cell factories: Analysis and engineering of phenotypic heterogeneity.
Binder D, Drepper T, Jaeger KE, Delvigne F, Wiechert W, Kohlheyer D, Grünberger A., Metab Eng 42(), 2017
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Binder D, Drepper T, Jaeger KE, Delvigne F, Wiechert W, Kohlheyer D, Grünberger A., Metab Eng 42(), 2017
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Present and Future of Culturing Bacteria.
Overmann J, Abt B, Sikorski J., Annu Rev Microbiol 71(), 2017
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Comparative Single-Cell Analysis of Different E. coli Expression Systems during Microfluidic Cultivation.
Binder D, Probst C, Grünberger A, Hilgers F, Loeschcke A, Jaeger KE, Kohlheyer D, Drepper T., PLoS One 11(8), 2016
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Binder D, Probst C, Grünberger A, Hilgers F, Loeschcke A, Jaeger KE, Kohlheyer D, Drepper T., PLoS One 11(8), 2016
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Current challenges of research on filamentous fungi in relation to human welfare and a sustainable bio-economy: a white paper.
Meyer V, Andersen MR, Brakhage AA, Braus GH, Caddick MX, Cairns TC, de Vries RP, Haarmann T, Hansen K, Hertz-Fowler C, Krappmann S, Mortensen UH, Peñalva MA, Ram AFJ, Head RM., Fungal Biol Biotechnol 3(), 2016
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Meyer V, Andersen MR, Brakhage AA, Braus GH, Caddick MX, Cairns TC, de Vries RP, Haarmann T, Hansen K, Hertz-Fowler C, Krappmann S, Mortensen UH, Peñalva MA, Ram AFJ, Head RM., Fungal Biol Biotechnol 3(), 2016
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Modeling and CFD simulation of nutrient distribution in picoliter bioreactors for bacterial growth studies on single-cell level.
Westerwalbesloh C, Grünberger A, Stute B, Weber S, Wiechert W, Kohlheyer D, von Lieres E., Lab Chip 15(21), 2015
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Westerwalbesloh C, Grünberger A, Stute B, Weber S, Wiechert W, Kohlheyer D, von Lieres E., Lab Chip 15(21), 2015
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Krämer CE, Singh A, Helfrich S, Grünberger A, Wiechert W, Nöh K, Kohlheyer D., PLoS One 10(10), 2015
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Krämer CE, Singh A, Helfrich S, Grünberger A, Wiechert W, Nöh K, Kohlheyer D., PLoS One 10(10), 2015
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Dawson CC, Intapa C, Jabra-Rizk MA., PLoS Pathog. 7(7), 2011
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Boulineau S, Tostevin F, Kiviet DJ, ten Wolde PR, Nghe P, Tans SJ., PLoS ONE 8(4), 2013
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Improvement in the lag phase estimation of individual cells that have survived mild heat treatment
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Aguirre JS, Monis A, Garcia de Fernando GD., International journal of food science and technology. 49(3), 2014
PMID: IND500731996
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