Photocaged Arabinose - A Novel Optogenetic Switch for Rapid and Gradual Control of Microbial Gene Expression

Binder D, Bier C, Grünberger A, Drobietz D, Hage-Hülsmann J, rey G, Büchs J, Kohlheyer D, Loeschcke A, Wiechert W, Jaeger K-E, et al. (2016)
ChemBioChem 17(4): 296-299.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Controlling cellular functions by light allows simple triggering of biological processes in a non-invasive fashion with high spatiotemporal resolution. In this context, light-regulated gene expression has enormous potential for achieving optogenetic control over almost any cellular process. Here, we report on two novel one-step cleavable photocaged arabinose compounds, which were applied as light-sensitive inducers of transcription in bacteria. Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production, as demonstrated for GFP and the complete violacein biosynthetic pathway. Moreover, single-cell analysis revealed that intrinsic heterogeneity of arabinose-mediated induction of gene expression was overcome when using photocaged arabinose. We have thus established a novel phototrigger for synthetic bio(techno)logy applications that enables precise and homogeneous control of bacterial target gene expression.
Erscheinungsjahr
2016
Zeitschriftentitel
ChemBioChem
Band
17
Ausgabe
4
Seite(n)
296-299
ISSN
1439-4227
Page URI
https://pub.uni-bielefeld.de/record/2912544

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Binder D, Bier C, Grünberger A, et al. Photocaged Arabinose - A Novel Optogenetic Switch for Rapid and Gradual Control of Microbial Gene Expression. ChemBioChem. 2016;17(4):296-299.
Binder, D., Bier, C., Grünberger, A., Drobietz, D., Hage-Hülsmann, J., rey, G., Büchs, J., et al. (2016). Photocaged Arabinose - A Novel Optogenetic Switch for Rapid and Gradual Control of Microbial Gene Expression. ChemBioChem, 17(4), 296-299. doi:10.1002/cbic.201500609
Binder, D., Bier, C., Grünberger, A., Drobietz, D., Hage-Hülsmann, J., rey, G., Büchs, J., Kohlheyer, D., Loeschcke, A., Wiechert, W., et al. (2016). Photocaged Arabinose - A Novel Optogenetic Switch for Rapid and Gradual Control of Microbial Gene Expression. ChemBioChem 17, 296-299.
Binder, D., et al., 2016. Photocaged Arabinose - A Novel Optogenetic Switch for Rapid and Gradual Control of Microbial Gene Expression. ChemBioChem, 17(4), p 296-299.
D. Binder, et al., “Photocaged Arabinose - A Novel Optogenetic Switch for Rapid and Gradual Control of Microbial Gene Expression”, ChemBioChem, vol. 17, 2016, pp. 296-299.
Binder, D., Bier, C., Grünberger, A., Drobietz, D., Hage-Hülsmann, J., rey, G., Büchs, J., Kohlheyer, D., Loeschcke, A., Wiechert, W., Jaeger, K.-E., Pietruszka, J., Drepper, T.: Photocaged Arabinose - A Novel Optogenetic Switch for Rapid and Gradual Control of Microbial Gene Expression. ChemBioChem. 17, 296-299 (2016).
Binder, Dennis, Bier, Claus, Grünberger, Alexander, Drobietz, Dagmar, Hage-Hülsmann, Jennifer, rey, Georg, Büchs, Jochen, Kohlheyer, Dietrich, Loeschcke, Anita, Wiechert, Wolfgang, Jaeger, Karl-Erich, Pietruszka, Jörg, and Drepper, Thomas. “Photocaged Arabinose - A Novel Optogenetic Switch for Rapid and Gradual Control of Microbial Gene Expression”. ChemBioChem 17.4 (2016): 296-299.

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