The lipopolysaccharide of the crop pathogen Xanthomonas translucens pv. translucens: chemical characterization and determination of signaling events in plant cells

Steffens T, Duda K, Lindner B, Vorhölter F-J, Bednarz H, Niehaus K, Holst O (2017)
GLYCOBIOLOGY 27(3): 264-274.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
Xanthomonas translucens pv. translucens (Xtt) is a Gram-negative pathogen of crops from the plant family Poaceae. The lipopolysaccharide (LPS) of Xtt was isolated and chemically characterized. The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0). The rough type of LPS (lipooligosaccharides; LOS) was isolated and its composition determined utilizing mass spectrometry. The structure of core-lipid A backbone was revealed by nuclear magnetic resonance (NMR) spectroscopy performed on O-deacylated LOS sample, and was shown to be:alpha-D-Manp-(1 -> 3)-alpha-D-Manp-(1 -> 3)-beta-D-Glcp-(1 -> 4)-alpha-D-Manp-(1 -> 5)-alpha-Kdo-(2 -> 6)-beta-D-GlcpN-(1 -> 6)-alpha-D-GlcpN. 4-alpha-Man and Kdo were further substituted via phosphodiester groups by two galactopyranuronic acids. Xtt LPS elicited a stress response in Nicotiana tabacum suspension cell cultures, namely a transient calcium signal and the generation of H2O2 was observed. Pharmacological studies indicated the involvement of plasma membrane calcium channels, kinases and phospholipase C as key factors in Xtt LPS induced pathogen signaling.
Erscheinungsjahr
Zeitschriftentitel
GLYCOBIOLOGY
Band
27
Ausgabe
3
Seite(n)
264-274
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Steffens T, Duda K, Lindner B, et al. The lipopolysaccharide of the crop pathogen Xanthomonas translucens pv. translucens: chemical characterization and determination of signaling events in plant cells. GLYCOBIOLOGY. 2017;27(3):264-274.
Steffens, T., Duda, K., Lindner, B., Vorhölter, F. - J., Bednarz, H., Niehaus, K., & Holst, O. (2017). The lipopolysaccharide of the crop pathogen Xanthomonas translucens pv. translucens: chemical characterization and determination of signaling events in plant cells. GLYCOBIOLOGY, 27(3), 264-274. doi:10.1093/glycob/cww093
Steffens, T., Duda, K., Lindner, B., Vorhölter, F. - J., Bednarz, H., Niehaus, K., and Holst, O. (2017). The lipopolysaccharide of the crop pathogen Xanthomonas translucens pv. translucens: chemical characterization and determination of signaling events in plant cells. GLYCOBIOLOGY 27, 264-274.
Steffens, T., et al., 2017. The lipopolysaccharide of the crop pathogen Xanthomonas translucens pv. translucens: chemical characterization and determination of signaling events in plant cells. GLYCOBIOLOGY, 27(3), p 264-274.
T. Steffens, et al., “The lipopolysaccharide of the crop pathogen Xanthomonas translucens pv. translucens: chemical characterization and determination of signaling events in plant cells”, GLYCOBIOLOGY, vol. 27, 2017, pp. 264-274.
Steffens, T., Duda, K., Lindner, B., Vorhölter, F.-J., Bednarz, H., Niehaus, K., Holst, O.: The lipopolysaccharide of the crop pathogen Xanthomonas translucens pv. translucens: chemical characterization and determination of signaling events in plant cells. GLYCOBIOLOGY. 27, 264-274 (2017).
Steffens, Tim, Duda, Katarzyna, Lindner, Buko, Vorhölter, Frank-Jörg, Bednarz, Hanna, Niehaus, Karsten, and Holst, Otto. “The lipopolysaccharide of the crop pathogen Xanthomonas translucens pv. translucens: chemical characterization and determination of signaling events in plant cells”. GLYCOBIOLOGY 27.3 (2017): 264-274.

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Comparative analysis of different xanthan samples by atomic force microscopy.
Teckentrup J, Al-Hammood O, Steffens T, Bednarz H, Walhorn V, Niehaus K, Anselmetti D., J Biotechnol 257(), 2017
PMID: 27919690

45 References

Daten bereitgestellt von Europe PubMed Central.

Bacterial lipopolysaccharides and innate immunity.
Alexander C, Rietschel ET., J. Endotoxin Res. 7(3), 2001
PMID: 11581570

AUTHOR UNKNOWN, 0

AUTHOR UNKNOWN, 0
Structural analysis and involvement in plant innate immunity of Xanthomonas axonopodis pv. citri lipopolysaccharide.
Casabuono A, Petrocelli S, Ottado J, Orellano EG, Couto AS., J. Biol. Chem. 286(29), 2011
PMID: 21596742

AUTHOR UNKNOWN, 0
Proteomic profiling of cellular targets of lipopolysaccharide-induced signalling in Nicotiana tabacum BY-2 cells.
Gerber IB, Laukens K, De Vijlder T, Witters E, Dubery IA., Biochim. Biophys. Acta 1784(11), 2008
PMID: 18638580
Determination of the absolute configuration of mono-saccharides in complex carbohydrates by capillary G.L.C.
Gerwig GJ, Kamerling JP, Vliegenthart JF., Carbohydr. Res. 77(), 1979
PMID: 519653
Flagellin perception: a paradigm for innate immunity.
Gomez-Gomez L, Boller T., Trends Plant Sci. 7(6), 2002
PMID: 12049921

AUTHOR UNKNOWN, 0

AUTHOR UNKNOWN, 0

AUTHOR UNKNOWN, 0

AUTHOR UNKNOWN, 0
The structure of lipopolysaccharides of gram-negative bacteria. II. The structure of the core region: a review
Knirel, Biokhimya 58(), 1993

AUTHOR UNKNOWN, 0
Inositol trisphosphate receptor in higher plants: is it real?
Krinke O, Novotna Z, Valentova O, Martinec J., J. Exp. Bot. 58(3), 2006
PMID: 17150991
E2F1 regulates cellular growth by mTORC1 signaling.
Real S, Meo-Evoli N, Espada L, Tauler A., PLoS ONE 6(1), 2011
PMID: 21283628
The structure of the lipooligosaccharide from Xanthomonas oryzae pv. Oryzae: the causal agent of the bacterial leaf blight in rice.
Di Lorenzo F, Palmigiano A, Silipo A, Desaki Y, Garozzo D, Lanzetta R, Shibuya N, Molinaro A., Carbohydr. Res. 427(), 2016
PMID: 27085742
Identification of Ca2+-stimulated polyphosphoinositide phospholipase C in isolated plant plasma membranes.
Melin PM, Sommarin M, Sandelius AS, Jergil B., FEBS Lett. 223(1), 1987
PMID: 2822482
Isolation and characterisation of the lipopolysaccharide from Xanthomonas hortorum pv. vitians.
Molinaro A, Lanzetta R, Evidente A, Parrilli M, Holst O., FEMS Microbiol. Lett. 181(1), 1999
PMID: 10564788
Structural elucidation of the O-chain of the lipopolysaccharide from Xanthomonas campestris strain 8004.
Molinaro A, Silipo A, Lanzetta R, Newman MA, Dow JM, Parrilli M., Carbohydr. Res. 338(3), 2003
PMID: 12543561
Sensing Gram-negative bacteria: a phylogenetic perspective.
Neyen C, Lemaitre B., Curr. Opin. Immunol. 38(), 2015
PMID: 26569344
Identification of a lipopolysaccharide responsive erk-like MAP kinase in tobacco leaf tissue.
Piater LA, Nurnberger T, Dubery IA., Mol. Plant Pathol. 5(4), 2004
PMID: IND43639183
Biochemistry of endotoxins.
Raetz CR., Annu. Rev. Biochem. 59(), 1990
PMID: 1695830
Lipopolysaccharide endotoxins.
Raetz CR, Whitfield C., Annu. Rev. Biochem. 71(), 2001
PMID: 12045108
A lectin S-domain receptor kinase mediates lipopolysaccharide sensing in Arabidopsis thaliana.
Ranf S, Gisch N, Schaffer M, Illig T, Westphal L, Knirel YA, Sanchez-Carballo PM, Zahringer U, Huckelhoven R, Lee J, Scheel D., Nat. Immunol. 16(4), 2015
PMID: 25729922
Plant phosphoinositide-specific phospholipase C: an insight.
Rupwate SD, Rajasekharan R., Plant Signal Behav 7(10), 2012
PMID: 22902702

AUTHOR UNKNOWN, 0
Multi-hit inhibition of circulating and cell-associated components of the toll-like receptor 4 pathway by oxidized phospholipids.
von Schlieffen E, Oskolkova OV, Schabbauer G, Gruber F, Bluml S, Genest M, Kadl A, Marsik C, Knapp S, Chow J, Leitinger N, Binder BR, Bochkov VN., Arterioscler. Thromb. Vasc. Biol. 29(3), 2008
PMID: 19112167
The elicitation of plant innate immunity by lipooligosaccharide of Xanthomonas campestris.
Silipo A, Molinaro A, Sturiale L, Dow JM, Erbs G, Lanzetta R, Newman MA, Parrilli M., J. Biol. Chem. 280(39), 2005
PMID: 16048996
The influence of a modified lipopolysaccharide O-antigen on the biosynthesis of xanthan in Xanthomonas campestris pv. campestris B100.
Steffens T, Vorholter FJ, Giampa M, Hublik G, Puhler A, Niehaus K., BMC Microbiol. 16(), 2016
PMID: 27215401
Phosphatidic acid: a multifunctional stress signaling lipid in plants.
Testerink C, Munnik T., Trends Plant Sci. 10(8), 2005
PMID: 16023886

AUTHOR UNKNOWN, 0
Involvement of phospholipases C and D in the defence responses of riboflavin-treated tobacco cells.
Wang L, Zhu X, Liu J, Chu X, Jiao J, Liang Y., Protoplasma 250(2), 2012
PMID: 22684579
Bacterial lipopolysaccharides. Extraction with phenol-water and further applications of the procedure
Westphal, Methods Carbohydr Chem 5(), 1965

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