An influence of the copy number of biosynthetic gene clusters on the production level of antibiotics in a heterologous host

Manderscheid N, Bilyk B, Busche T, Kalinowski J, Paululat T, Bechthold A, Petzke L, Luzhetskyy A (2016)
JOURNAL OF BIOTECHNOLOGY 232: 110-117.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor/in
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Abstract / Bemerkung
Streptomyces albus J1074 is a well-known host for heterologous expression of secondary metabolites. To further increase its potential and to study the influence of cluster multiplication, additional phi C31 attachment site was integrated into its genome using a system for transposon mutagenesis. Four secondary metabolite clusters were expressed in strains with different numbers of attachment sites, ranging from one to three copies of the site. Secondary metabolite production was examined and a new compound could be detected, purified and its structure was elucidated. (C) 2016 Elsevier B.V. All rights reserved.
Stichworte
Heterologous expression; Antibiotic production; Strain development; Streptomyces albus J1074; phi C31-encoded recombination system; Multiple; attachment sites
Erscheinungsjahr
2016
Zeitschriftentitel
JOURNAL OF BIOTECHNOLOGY
Band
232
Seite(n)
110-117
Konferenz
10th CeBiTec Symposium on Bioinformatics for Biotechnology and Biomedicine
Konferenzort
Bielefeld Univ, Bielefeld, GERMANY
ISSN
0168-1656
eISSN
1873-4863
Page URI
https://pub.uni-bielefeld.de/record/2905633

Zitieren

Manderscheid N, Bilyk B, Busche T, et al. An influence of the copy number of biosynthetic gene clusters on the production level of antibiotics in a heterologous host. JOURNAL OF BIOTECHNOLOGY. 2016;232:110-117.
Manderscheid, N., Bilyk, B., Busche, T., Kalinowski, J., Paululat, T., Bechthold, A., Petzke, L., et al. (2016). An influence of the copy number of biosynthetic gene clusters on the production level of antibiotics in a heterologous host. JOURNAL OF BIOTECHNOLOGY, 232, 110-117. doi:10.1016/j.jbiotec.2016.05.038
Manderscheid, N., Bilyk, B., Busche, T., Kalinowski, J., Paululat, T., Bechthold, A., Petzke, L., and Luzhetskyy, A. (2016). An influence of the copy number of biosynthetic gene clusters on the production level of antibiotics in a heterologous host. JOURNAL OF BIOTECHNOLOGY 232, 110-117.
Manderscheid, N., et al., 2016. An influence of the copy number of biosynthetic gene clusters on the production level of antibiotics in a heterologous host. JOURNAL OF BIOTECHNOLOGY, 232, p 110-117.
N. Manderscheid, et al., “An influence of the copy number of biosynthetic gene clusters on the production level of antibiotics in a heterologous host”, JOURNAL OF BIOTECHNOLOGY, vol. 232, 2016, pp. 110-117.
Manderscheid, N., Bilyk, B., Busche, T., Kalinowski, J., Paululat, T., Bechthold, A., Petzke, L., Luzhetskyy, A.: An influence of the copy number of biosynthetic gene clusters on the production level of antibiotics in a heterologous host. JOURNAL OF BIOTECHNOLOGY. 232, 110-117 (2016).
Manderscheid, Niko, Bilyk, Bohdan, Busche, Tobias, Kalinowski, Jörn, Paululat, Thomas, Bechthold, Andreas, Petzke, Lutz, and Luzhetskyy, Andriy. “An influence of the copy number of biosynthetic gene clusters on the production level of antibiotics in a heterologous host”. JOURNAL OF BIOTECHNOLOGY 232 (2016): 110-117.

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