An influence of the copy number of biosynthetic gene clusters on the production level of antibiotics in a heterologous host
Manderscheid N, Bilyk B, Busche T, Kalinowski J, Paululat T, Bechthold A, Petzke L, Luzhetskyy A (2016)
JOURNAL OF BIOTECHNOLOGY 232: 110-117.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
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Autor*in
Manderscheid, Niko;
Bilyk, Bohdan;
Busche, Tobias;
Kalinowski, JörnUniBi;
Paululat, Thomas;
Bechthold, Andreas;
Petzke, Lutz;
Luzhetskyy, Andriy
Abstract / Bemerkung
Streptomyces albus J1074 is a well-known host for heterologous expression of secondary metabolites. To further increase its potential and to study the influence of cluster multiplication, additional phi C31 attachment site was integrated into its genome using a system for transposon mutagenesis. Four secondary metabolite clusters were expressed in strains with different numbers of attachment sites, ranging from one to three copies of the site. Secondary metabolite production was examined and a new compound could be detected, purified and its structure was elucidated. (C) 2016 Elsevier B.V. All rights reserved.
Stichworte
Heterologous expression;
Antibiotic production;
Strain development;
Streptomyces albus J1074;
phi C31-encoded recombination system;
Multiple;
attachment sites
Erscheinungsjahr
2016
Zeitschriftentitel
JOURNAL OF BIOTECHNOLOGY
Band
232
Seite(n)
110-117
Konferenz
10th CeBiTec Symposium on Bioinformatics for Biotechnology and Biomedicine
Konferenzort
Bielefeld Univ, Bielefeld, GERMANY
ISSN
0168-1656
eISSN
1873-4863
Page URI
https://pub.uni-bielefeld.de/record/2905633
Zitieren
Manderscheid N, Bilyk B, Busche T, et al. An influence of the copy number of biosynthetic gene clusters on the production level of antibiotics in a heterologous host. JOURNAL OF BIOTECHNOLOGY. 2016;232:110-117.
Manderscheid, N., Bilyk, B., Busche, T., Kalinowski, J., Paululat, T., Bechthold, A., Petzke, L., et al. (2016). An influence of the copy number of biosynthetic gene clusters on the production level of antibiotics in a heterologous host. JOURNAL OF BIOTECHNOLOGY, 232, 110-117. doi:10.1016/j.jbiotec.2016.05.038
Manderscheid, Niko, Bilyk, Bohdan, Busche, Tobias, Kalinowski, Jörn, Paululat, Thomas, Bechthold, Andreas, Petzke, Lutz, and Luzhetskyy, Andriy. 2016. “An influence of the copy number of biosynthetic gene clusters on the production level of antibiotics in a heterologous host”. JOURNAL OF BIOTECHNOLOGY 232: 110-117.
Manderscheid, N., Bilyk, B., Busche, T., Kalinowski, J., Paululat, T., Bechthold, A., Petzke, L., and Luzhetskyy, A. (2016). An influence of the copy number of biosynthetic gene clusters on the production level of antibiotics in a heterologous host. JOURNAL OF BIOTECHNOLOGY 232, 110-117.
Manderscheid, N., et al., 2016. An influence of the copy number of biosynthetic gene clusters on the production level of antibiotics in a heterologous host. JOURNAL OF BIOTECHNOLOGY, 232, p 110-117.
N. Manderscheid, et al., “An influence of the copy number of biosynthetic gene clusters on the production level of antibiotics in a heterologous host”, JOURNAL OF BIOTECHNOLOGY, vol. 232, 2016, pp. 110-117.
Manderscheid, N., Bilyk, B., Busche, T., Kalinowski, J., Paululat, T., Bechthold, A., Petzke, L., Luzhetskyy, A.: An influence of the copy number of biosynthetic gene clusters on the production level of antibiotics in a heterologous host. JOURNAL OF BIOTECHNOLOGY. 232, 110-117 (2016).
Manderscheid, Niko, Bilyk, Bohdan, Busche, Tobias, Kalinowski, Jörn, Paululat, Thomas, Bechthold, Andreas, Petzke, Lutz, and Luzhetskyy, Andriy. “An influence of the copy number of biosynthetic gene clusters on the production level of antibiotics in a heterologous host”. JOURNAL OF BIOTECHNOLOGY 232 (2016): 110-117.
3 Zitationen in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
Chromosomal position effect influences the heterologous expression of genes and biosynthetic gene clusters in Streptomyces albus J1074.
Bilyk B, Horbal L, Luzhetskyy A., Microb Cell Fact 16(1), 2017
PMID: 28052753
Bilyk B, Horbal L, Luzhetskyy A., Microb Cell Fact 16(1), 2017
PMID: 28052753
Multiplexed site-specific genome engineering for overproducing bioactive secondary metabolites in actinomycetes.
Li L, Zheng G, Chen J, Ge M, Jiang W, Lu Y., Metab Eng 40(), 2017
PMID: 28088540
Li L, Zheng G, Chen J, Ge M, Jiang W, Lu Y., Metab Eng 40(), 2017
PMID: 28088540
New strategies and approaches for engineering biosynthetic gene clusters of microbial natural products.
Li L, Jiang W, Lu Y., Biotechnol Adv 35(8), 2017
PMID: 28323062
Li L, Jiang W, Lu Y., Biotechnol Adv 35(8), 2017
PMID: 28323062
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