Characterization of a collection of plasmid-containing bacteria isolated from an on-farm biopurification system used for pesticide removal

Martini MC, Albicoro FJ, Nour E, Schlüter A, van Elsas JD, Springael D, Smalla K, Pistorio M, Lagares A, Florencia Del Papa M (2015)
Plasmid 80(Special Issue S1): 16-23.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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DOI
Autor*in
Martini, Maria Carla; Albicoro, Francisco Javier; Nour, Eman; Schlüter, AndreasUniBi ; van Elsas, Jan Dirk; Springael, Dirk; Smalla, Kornelia; Pistorio, Mariano; Lagares, Antonio; Florencia Del Papa, Maria
Einrichtung
Centrum für Biotechnologie > Arbeitsgruppe A. Pühler
Abstract / Bemerkung
Biopurification systems (BPS) are complex soil-related and artificially-generated environments usually designed for the removal of toxic compounds from contaminated wastewaters. The present study has been conducted to isolate and characterize a collection of cultivable plasmid-carrying bacterial isolates recovered from a BPS established for the decontamination of wastewater generated in a farmyard. Out of 1400 isolates, a collection of 75 plasmid-containing bacteria was obtained, of which 35 representative isolates comprising in total at least 50 plasmids were chosen for further characterization. Bacterial hosts were taxonomically assigned by 16S ribosomal RNA gene sequencing and phenotypically characterized according to their ability to grow in presence of different antibiotics and heavy metals. The study demonstrated that a high proportion of the isolates was tolerant to antibiotics and/or heavy metals, highlighting the on-farm BPS enrichment in such genetic traits. Several plasmids conferring such resistances in the bacterial collection were detected to be either mobilizable or selftransmissible. Occurrence of broad host range plasmids of the incompatibility groups IncP, IncQ IncN and IncW was examined with positive results only for the first group. Presence of the IS1071 insertion sequence, frequently associated with xenobiotics degradation genes, was detected in DNA obtained from 24 of these isolates, strongly suggesting the presence of yet-hidden catabolic activities in the collection of isolates. The results showed a remarkable diversity in the plasmid mobilome of cultivable bacteria in the BPS with the presence of abundant resistance markers of different types, thus providing a suitable environment to investigate the genetic structure of the mobile genetic pool in a model on-farm biofilter for wastewater decontamination in intensive agricultural production. (C) 2015 Elsevier Inc. All rights reserved.
Stichworte
Pesticide; Plasmid; Mobile genetic element; Biopurification system
Erscheinungsjahr
2015
Zeitschriftentitel
Plasmid
Band
80
Ausgabe
Special Issue S1
Seite(n)
16-23
ISSN
0147-619X
Page URI
https://pub.uni-bielefeld.de/record/2773134

Zitieren

Martini MC, Albicoro FJ, Nour E, et al. Characterization of a collection of plasmid-containing bacteria isolated from an on-farm biopurification system used for pesticide removal. Plasmid. 2015;80(Special Issue S1):16-23.
Martini, M. C., Albicoro, F. J., Nour, E., Schlüter, A., van Elsas, J. D., Springael, D., Smalla, K., et al. (2015). Characterization of a collection of plasmid-containing bacteria isolated from an on-farm biopurification system used for pesticide removal. Plasmid, 80(Special Issue S1), 16-23. doi:10.1016/j.plasmid.2015.05.001
Martini, Maria Carla, Albicoro, Francisco Javier, Nour, Eman, Schlüter, Andreas, van Elsas, Jan Dirk, Springael, Dirk, Smalla, Kornelia, Pistorio, Mariano, Lagares, Antonio, and Florencia Del Papa, Maria. 2015. “Characterization of a collection of plasmid-containing bacteria isolated from an on-farm biopurification system used for pesticide removal”. Plasmid 80 (Special Issue S1): 16-23.
Martini, M. C., Albicoro, F. J., Nour, E., Schlüter, A., van Elsas, J. D., Springael, D., Smalla, K., Pistorio, M., Lagares, A., and Florencia Del Papa, M. (2015). Characterization of a collection of plasmid-containing bacteria isolated from an on-farm biopurification system used for pesticide removal. Plasmid 80, 16-23.
Martini, M.C., et al., 2015. Characterization of a collection of plasmid-containing bacteria isolated from an on-farm biopurification system used for pesticide removal. Plasmid, 80(Special Issue S1), p 16-23.
M.C. Martini, et al., “Characterization of a collection of plasmid-containing bacteria isolated from an on-farm biopurification system used for pesticide removal”, Plasmid, vol. 80, 2015, pp. 16-23.
Martini, M.C., Albicoro, F.J., Nour, E., Schlüter, A., van Elsas, J.D., Springael, D., Smalla, K., Pistorio, M., Lagares, A., Florencia Del Papa, M.: Characterization of a collection of plasmid-containing bacteria isolated from an on-farm biopurification system used for pesticide removal. Plasmid. 80, 16-23 (2015).
Martini, Maria Carla, Albicoro, Francisco Javier, Nour, Eman, Schlüter, Andreas, van Elsas, Jan Dirk, Springael, Dirk, Smalla, Kornelia, Pistorio, Mariano, Lagares, Antonio, and Florencia Del Papa, Maria. “Characterization of a collection of plasmid-containing bacteria isolated from an on-farm biopurification system used for pesticide removal”. Plasmid 80.Special Issue S1 (2015): 16-23.

5 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Novel environmental class 1 integrons and cassette arrays recovered from an on-farm bio-purification plant.
Martini MC, Quiroga MP, Pistorio M, Lagares A, Centrón D, Del Papa MF., FEMS Microbiol Ecol 94(3), 2018
PMID: 29300936
Host Range of the Conjugative Transfer System of IncP-9 Naphthalene-Catabolic Plasmid NAH7 and Characterization of Its oriT Region and Relaxase.
Kishida K, Inoue K, Ohtsubo Y, Nagata Y, Tsuda M., Appl Environ Microbiol 83(1), 2017
PMID: 27742684
Isolation of Viable but Non-culturable Bacteria from Printing and Dyeing Wastewater Bioreactor Based on Resuscitation Promoting Factor.
Jin Y, Gan G, Yu X, Wu D, Zhang L, Yang N, Hu J, Liu Z, Zhang L, Hong H, Yan X, Liang Y, Ding L, Pan Y., Curr Microbiol 74(7), 2017
PMID: 28417187
Groundwater contamination with 2,6-dichlorobenzamide (BAM) and perspectives for its microbial removal.
Ellegaard-Jensen L, Horemans B, Raes B, Aamand J, Hansen LH., Appl Microbiol Biotechnol 101(13), 2017
PMID: 28616645
Genomics of high molecular weight plasmids isolated from an on-farm biopurification system.
Martini MC, Wibberg D, Lozano M, Torres Tejerizo G, Albicoro FJ, Jaenicke S, van Elsas JD, Petroni A, Garcillán-Barcia MP, de la Cruz F, Schlüter A, Pühler A, Pistorio M, Lagares A, Del Papa MF., Sci Rep 6(), 2016
PMID: 27321040

56 References

Daten bereitgestellt von Europe PubMed Central.

Exogenous isolation of conjugative plasmids from pesticide contaminated soil
Anjum R, Grohmann E, Malik A., World J. Microbiol. Biotechnol. 28(2), 2012
PMID: IND44746167
All IncP-1 plasmid subgroups, including the novel epsilon subgroup, are prevalent in the influent of a Danish wastewater treatment plant.
Bahl MI, Burmolle M, Meisner A, Hansen LH, Sorensen SJ., Plasmid 62(2), 2009
PMID: 19501117
Co-selection of antibiotic and metal resistance.
Baker-Austin C, Wright MS, Stepanauskas R, McArthur JV., Trends Microbiol. 14(4), 2006
PMID: 16537105
R factor transfer in Rhizobium leguminosarum.
Beringer JE., J. Gen. Microbiol. 84(1), 1974
PMID: 4612098
Piggery manure used for soil fertilization is a reservoir for transferable antibiotic resistance plasmids.
Binh CT, Heuer H, Kaupenjohann M, Smalla K., FEMS Microbiol. Ecol. 66(1), 2008
PMID: 18557938
Complete nucleotide sequence of the IncN plasmid pKOX105 encoding VIM-1, QnrS1 and SHV-12 proteins in Enterobacteriaceae from Bolzano, Italy compared with IncN plasmids encoding KPC enzymes in the USA.
Carattoli A, Aschbacher R, March A, Larcher C, Livermore DM, Woodford N., J. Antimicrob. Chemother. 65(10), 2010
PMID: 20656680
Genetic and phenotypic diversity of parathion-degrading bacteria isolated from rice paddy soils.
Choi MK, Kim KD, Ahn KM, Shin DH, Hwang JH, Seong CN, Ka JO., J. Microbiol. Biotechnol. 19(12), 2009
PMID: 20075637
Transport and degradation of pesticides in a biopurification system under variable flux, Part I: a microcosm study
De, Environ. Pollut 158(10), 2010
Shifts in abundance and diversity of mobile genetic elements after the introduction of diverse pesticides into an on-farm biopurification system over the course of a year.
Dealtry S, Holmsgaard PN, Dunon V, Jechalke S, Ding GC, Krogerrecklenfort E, Heuer H, Hansen LH, Springael D, Zuhlke S, Sorensen SJ, Smalla K., Appl. Environ. Microbiol. 80(13), 2014
PMID: 24771027
Isolation and characterization of alfalfa-nodulating rhizobia present in acidic soils of central Argentina and Uruguay
Del, Appl. Environ. Microbiol 65(), 1999
The evolution of IncP catabolic plasmids.
Dennis JJ., Curr. Opin. Biotechnol. 16(3), 2005
PMID: 15961030
Genetic rearrangement of the atzAB atrazine-degrading gene cassette from pADP1::Tn5 to the chromosome of Variovorax sp. MD1 and MD2.
Devers M, Rouard N, Martin-Laurent F., Gene 392(1-2), 2006
PMID: 17188820
High prevalence of IncP-1 plasmids and IS1071 insertion sequences in on-farm biopurification systems and other pesticide-polluted environments.
Dunon V, Sniegowski K, Bers K, Lavigne R, Smalla K, Springael D., FEMS Microbiol. Ecol. 86(3), 2013
PMID: 23802695
A rapid method for the identification of plasmid desoxyribonucleic acid in bacteria.
Eckhardt T., Plasmid 1(4), 1978
PMID: 107540
Dynamics of the IncW genetic backbone imply general trends in conjugative plasmid evolution.
Fernandez-Lopez R, Garcillan-Barcia MP, Revilla C, Lazaro M, Vielva L, de la Cruz F., FEMS Microbiol. Rev. 30(6), 2006
PMID: 17026718
Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans.
Figurski DH, Helinski DR., Proc. Natl. Acad. Sci. U.S.A. 76(4), 1979
PMID: 377280
Second symbiotic megaplasmid in Rhizobium meliloti carrying exopolysaccharide and thiamine synthesis genes.
Finan TM, Kunkel B, De Vos GF, Signer ER., J. Bacteriol. 167(1), 1986
PMID: 3013840
Influence of humans on evolution and mobilization of environmental antibiotic resistome
Gaze, Emerg. Infect. Dis 19(7), 2013
Detection and characterization of broad-host-range plasmids in environmental bacteria by PCR.
Gotz A, Pukall R, Smit E, Tietze E, Prager R, Tschape H, van Elsas JD, Smalla K., Appl. Environ. Microbiol. 62(7), 1996
PMID: 8779598
Natural horizontal transfer of a naphthalene dioxygenase gene between bacteria native to a coal tar-contaminated field site.
Herrick JB, Stuart-Keil KG, Ghiorse WC, Madsen EL., Appl. Environ. Microbiol. 63(6), 1997
PMID: 9172352
Manure and sulfadiazine synergistically increased bacterial antibiotic resistance in soil over at least two months.
Heuer H, Smalla K., Environ. Microbiol. 9(3), 2007
PMID: 17298366
The two megaplasmids of Rhizobium meliloti are involved in the effective nodulation of alfalfa
Hynes, Mol. Gen. Genet 202(), 1986
Antimicrobial, heavy metal resistance and plasmid profile of coliforms isolated from nosocomial infections in a hospital in Isfahan, Iran
Karbasizaed, Afr. J. Biotechnol 2(), 2003
The molecular biology of Euglena gracilis. VII. Inorganic requirements for a minimal culture medium.
Kempner ES, Miller JH., J. Protozool. 19(2), 1972
PMID: 4624303
Widespread occurrence of the tfd-II genes in soil bacteria revealed by nucleotide sequence analysis of 2,4-dichlorophenoxyacetic acid degradative plasmids pDB1 and p712.
Kim DU, Kim MS, Lim JS, Ka JO., Plasmid 69(3), 2013
PMID: 23376020
Monitoring the diversity of Rhizobium meliloti field and microcosm isolates with a novel rapid genotyping method using insertion elements
Kosier, Mol. Ecol 2(), 1993
A SIMPLIFIED FUCHSIN SULPHITE (ENDO) AGAR.
Levine M., Am J Public Health (N Y) 8(11), 1918
PMID: 18009991
Chromosome-encoded gene cluster for the metabolic pathway that converts aniline to TCA-cycle intermediates in Delftia tsuruhatensis AD9.
Liang Q, Takeo M, Chen M, Zhang W, Xu Y, Lin M., Microbiology (Reading, Engl.) 151(Pt 10), 2005
PMID: 16207925
Diversity, biology and evolution of IncQ-family plasmids.
Loftie-Eaton W, Rawlings DE., Plasmid 67(1), 2011
PMID: 22037393
Nucleotide sequence of plasmid pCNB1 from comamonas strain CNB-1 reveals novel genetic organization and evolution for 4-chloronitrobenzene degradation.
Ma YF, Wu JF, Wang SY, Jiang CY, Zhang Y, Qi SW, Liu L, Zhao GP, Liu SJ., Appl. Environ. Microbiol. 73(14), 2007
PMID: 17526790
Lactose-Fermenting Bacteria in Faeces.
Macconkey A., J Hyg (Lond) 5(3), 1905
PMID: 20474229
Alcaligenes eutrophus CH34 is a facultative chemolithotroph with plasmid-bound resistance to heavy metals.
Mergeay M, Nies D, Schlegel HG, Gerits J, Charles P, Van Gijsegem F., J. Bacteriol. 162(1), 1985
PMID: 3884593
Assay of β-galacosidase
Miller, 1972
Wastewater bacterial communities bring together broad-host range plasmids, integrons and a wide diversity of uncharacterized gene cassettes.
Moura A, Henriques I, Smalla K, Correia A., Res. Microbiol. 161(1), 2009
PMID: 20004718
The antibiotic resistance "mobilome": searching for the link between environment and clinic.
Perry JA, Wright GD., Front Microbiol 4(), 2013
PMID: 23755047
Conjugal properties of the Sinorhizobium meliloti plasmid mobilome.
Pistorio M, Giusti MA, Del Papa MF, Draghi WO, Lozano MJ, Tejerizo GT, Lagares A., FEMS Microbiol. Ecol. 65(3), 2008
PMID: 18537840
The copy-number of plasmids and other genetic elements can be determined by SYBR-Green-based quantitative real-time PCR.
Providenti MA, O'Brien JM, Ewing RJ, Paterson ES, Smith ML., J. Microbiol. Methods 65(3), 2005
PMID: 16216354
Isolation and characterization of heavy metal resistant bacteria from Barak River contaminated with pulp paper mill effluent, South Assam.
Rajkumar B, Sharma GD, Paul AK., Bull Environ Contam Toxicol 89(2), 2012
PMID: 22617943
Comparative biology of IncQ and IncQ-like plasmids.
Rawlings DE, Tietze E., Microbiol. Mol. Biol. Rev. 65(4), 2001
PMID: 11729261

Sambrook, 1989
Genomics of IncP-1 antibiotic resistance plasmids isolated from wastewater treatment plants provides evidence for a widely accessible drug resistance gene pool.
Schluter A, Szczepanowski R, Puhler A, Top EM., FEMS Microbiol. Rev. 31(4), 2007
PMID: 17553065
Heavy metal driven co-selection of antibiotic resistance in soil and water bodies impacted by agriculture and aquaculture.
Seiler C, Berendonk TU., Front Microbiol 3(), 2012
PMID: 23248620
Identification and characterization of integron mediated antibiotic resistance in pentachlorophenol degrading bacterium isolated from the chemostat.
Sharma A, Thakur IS., J Environ Sci (China) 21(6), 2009
PMID: 19803095
Genetic and phenotypic diversity of carbofuran-degrading bacteria isolated from agricultural soils.
Shin DH, Kim DU, Seong CN, Song HG, Ka JO., J. Microbiol. Biotechnol. 22(4), 2012
PMID: 22534290
Exogenous isolation of antibiotic resistance plasmids from piggery manure slurries reveals a high prevalence and diversity of IncQ-like plasmids.
Smalla K, Heuer H, Gotz A, Niemeyer D, Krogerrecklenfort E, Tietze E., Appl. Environ. Microbiol. 66(11), 2000
PMID: 11055935
Increased abundance of IncP-1beta plasmids and mercury resistance genes in mercury-polluted river sediments: first discovery of IncP-1beta plasmids with a complex mer transposon as the sole accessory element.
Smalla K, Haines AS, Jones K, Krogerrecklenfort E, Heuer H, Schloter M, Thomas CM., Appl. Environ. Microbiol. 72(11), 2006
PMID: 16980416
Self-transmissible mercury resistance plasmids with gene-mobilizing capacity in soil bacterial populations: influence of wheat roots and mercury addition.
Smit E, Wolters A, van Elsas JD., Appl. Environ. Microbiol. 64(4), 1998
PMID: 9546155
Improvement of pesticide mineralization in on-farm biopurification systems by bioaugmentation with pesticide-primed soil.
Sniegowski K, Bers K, Van Goetem K, Ryckeboer J, Jaeken P, Spanoghe P, Springael D., FEMS Microbiol. Ecol. 76(1), 2011
PMID: 21231937
The lin genes for γ-hexachlorocyclohexane degradation in Sphingomonas sp. MM-1 proved to be dispersed across multiple plasmids.
Tabata M, Endo R, Ito M, Ohtsubo Y, Kumar A, Tsuda M, Nagata Y., Biosci. Biotechnol. Biochem. 75(3), 2011
PMID: 21389627
Incompatibility group P plasmids: genetics, evolution, and use in genetic manipulation.
Thomas CM, Smith CA., Annu. Rev. Microbiol. 41(), 1987
PMID: 3318684
The role of mobile genetic elements in bacterial adaptation to xenobiotic organic compounds.
Top EM, Springael D., Curr. Opin. Biotechnol. 14(3), 2003
PMID: 12849778
The completely sequenced plasmid pEST4011 contains a novel IncP1 backbone and a catabolic transposon harboring tfd genes for 2,4-dichlorophenoxyacetic acid degradation.
Vedler E, Vahter M, Heinaru A., J. Bacteriol. 186(21), 2004
PMID: 15489427
Diversity of uncultured microorganisms associated with the seagrass Halophila stipulacea estimated by restriction fragment length polymorphism analysis of PCR-amplified 16S rRNA genes.
Weidner S, Arnold W, Puhler A., Appl. Environ. Microbiol. 62(3), 1996
PMID: 8975607
Polycyclic aromatic hydrocarbon degrading gene islands in five pyrene-degrading Mycobacterium isolates from different geographic locations.
Zhang C, Anderson AJ., Can. J. Microbiol. 58(1), 2011
PMID: 22188370
Plasmid-mediated bioaugmentation for the degradation of chlorpyrifos in soil
Zhang, J. Hazard. Mater 221–222(), 2012
blaCTX-M-65 is carried by a Tn1722-like element on an IncN conjugative plasmid of ST131 Escherichia coli.
Zong Z, Yu R, Wang X, Lu X., J. Med. Microbiol. 60(Pt 4), 2010
PMID: 21163826
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