Targeted expression of nuclear transgenes in Chlamydomonas reinhardtii with a versatile, modular vector toolkit

Lauersen KJ, Kruse O, Mussgnug JH (2015)
Applied Microbiology and Biotechnology 99(8): 3491-3503.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
OA 1.87 MB
DOI
URN
urn:nbn:de:0070-pub-27123155
Autor*in
Lauersen, Kyle J.UniBi ; Kruse, OlafUniBi ; Mussgnug, Jan H.UniBi
Einrichtung
Centrum für Biotechnologie > Institut für Biochemie und Biotechnik
Fakultät für Biologie > Algenbiotechnologie
Centrum für Biotechnologie > Arbeitsgruppe O. Kruse
Centrum für Biotechnologie > Graduate Center > Graduate Cluster Industrial Biotechnology
Erscheinungsjahr
2015
Zeitschriftentitel
Applied Microbiology and Biotechnology
Band
99
Ausgabe
8
Seite(n)
3491-3503
Urheberrecht / Lizenzen
Creative Commons Namensnennung 3.0 Unported (CC BY 3.0)
ISSN
0175-7598
Page URI
https://pub.uni-bielefeld.de/record/2712315

Zitieren

Lauersen KJ, Kruse O, Mussgnug JH. Targeted expression of nuclear transgenes in Chlamydomonas reinhardtii with a versatile, modular vector toolkit. Applied Microbiology and Biotechnology. 2015;99(8):3491-3503.
Lauersen, K. J., Kruse, O., & Mussgnug, J. H. (2015). Targeted expression of nuclear transgenes in Chlamydomonas reinhardtii with a versatile, modular vector toolkit. Applied Microbiology and Biotechnology, 99(8), 3491-3503. doi:10.1007/s00253-014-6354-7
Lauersen, Kyle J., Kruse, Olaf, and Mussgnug, Jan H. 2015. “Targeted expression of nuclear transgenes in Chlamydomonas reinhardtii with a versatile, modular vector toolkit”. Applied Microbiology and Biotechnology 99 (8): 3491-3503.
Lauersen, K. J., Kruse, O., and Mussgnug, J. H. (2015). Targeted expression of nuclear transgenes in Chlamydomonas reinhardtii with a versatile, modular vector toolkit. Applied Microbiology and Biotechnology 99, 3491-3503.
Lauersen, K.J., Kruse, O., & Mussgnug, J.H., 2015. Targeted expression of nuclear transgenes in Chlamydomonas reinhardtii with a versatile, modular vector toolkit. Applied Microbiology and Biotechnology, 99(8), p 3491-3503.
K.J. Lauersen, O. Kruse, and J.H. Mussgnug, “Targeted expression of nuclear transgenes in Chlamydomonas reinhardtii with a versatile, modular vector toolkit”, Applied Microbiology and Biotechnology, vol. 99, 2015, pp. 3491-3503.
Lauersen, K.J., Kruse, O., Mussgnug, J.H.: Targeted expression of nuclear transgenes in Chlamydomonas reinhardtii with a versatile, modular vector toolkit. Applied Microbiology and Biotechnology. 99, 3491-3503 (2015).
Lauersen, Kyle J., Kruse, Olaf, and Mussgnug, Jan H. “Targeted expression of nuclear transgenes in Chlamydomonas reinhardtii with a versatile, modular vector toolkit”. Applied Microbiology and Biotechnology 99.8 (2015): 3491-3503.
Alle Dateien verfügbar unter der/den folgenden Lizenz(en):
Creative Commons Namensnennung 3.0 Unported (CC BY 3.0):
cc_by_3_0
https://creativecommons.org/licenses/by/3.0/deed.de
https://creativecommons.org/licenses/by/3.0/legalcode.de
Volltext(e)
Name
1.87 MB
Access Level
OA Open Access
Zuletzt Hochgeladen
2019-09-06T09:18:29Z
MD5 Prüfsumme
ad8fe11e413b45fec2a063e6146e1765


28 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Evaluation of strategies for improving the transgene expression in an oleaginous microalga Scenedesmus acutus.
Suttangkakul A, Sirikhachornkit A, Juntawong P, Puangtame W, Chomtong T, Srifa S, Sathitnaitham S, Dumrongthawatchai W, Jariyachawalid K, Vuttipongchaikij S., BMC Biotechnol 19(1), 2019
PMID: 30630453
Secretion of Acetylxylan Esterase From Chlamydomonas reinhardtii Enables Utilization of Lignocellulosic Biomass as a Carbon Source.
Ramos-Martinez EM, Fimognari L, Rasmussen MK, Sakuragi Y., Front Bioeng Biotechnol 7(), 2019
PMID: 30873405
Characterization of the GPR1/FUN34/YaaH protein family in the green microalga Chlamydomonas suggests their role as intracellular membrane acetate channels.
Durante L, Hübner W, Lauersen KJ, Remacle C., Plant Direct 3(6), 2019
PMID: 31245784
Using YFP as a Reporter of Gene Expression in the Green Alga Chlamydomonas reinhardtii.
Blaby-Haas CE, Page MD, Merchant SS., Methods Mol Biol 1755(), 2018
PMID: 29671268
High-resolution suborganellar localization of Ca2+-binding protein CAS, a novel regulator of CO2-concentrating mechanism.
Yamano T, Toyokawa C, Fukuzawa H., Protoplasma 255(4), 2018
PMID: 29372336
Comparison of secretory signal peptides for heterologous protein expression in microalgae: Expanding the secretion portfolio for Chlamydomonas reinhardtii.
Molino JVD, de Carvalho JCM, Mayfield SP., PLoS One 13(2), 2018
PMID: 29408937
Enhancing heterologous expression in Chlamydomonas reinhardtii by transcript sequence optimization.
Weiner I, Atar S, Schweitzer S, Eilenberg H, Feldman Y, Avitan M, Blau M, Danon A, Tuller T, Yacoby I., Plant J 94(1), 2018
PMID: 29383789
Recent developments in synthetic biology and metabolic engineering in microalgae towards biofuel production.
Jagadevan S, Banerjee A, Banerjee C, Guria C, Tiwari R, Baweja M, Shukla P., Biotechnol Biofuels 11(), 2018
PMID: 29988523
Intron-containing algal transgenes mediate efficient recombinant gene expression in the green microalga Chlamydomonas reinhardtii.
Baier T, Wichmann J, Kruse O, Lauersen KJ., Nucleic Acids Res 46(13), 2018
PMID: 30053227
Selectable Markers and Reporter Genes for Engineering the Chloroplast of Chlamydomonas reinhardtii.
Esland L, Larrea-Alvarez M, Purton S., Biology (Basel) 7(4), 2018
PMID: 30309004
Two-component cyclase opsins of green algae are ATP-dependent and light-inhibited guanylyl cyclases.
Tian Y, Gao S, von der Heyde EL, Hallmann A, Nagel G., BMC Biol 16(1), 2018
PMID: 30522480
A novel bicistronic expression system composed of the intraflagellar transport protein gene ift25 and FMDV 2A sequence directs robust nuclear gene expression in Chlamydomonas reinhardtii.
Dong B, Hu HH, Li ZF, Cheng RQ, Meng DM, Wang J, Fan ZC., Appl Microbiol Biotechnol 101(10), 2017
PMID: 28238082
Nuclear transformation and functional gene expression in the oleaginous microalga Monoraphidium neglectum.
Jaeger D, Hübner W, Huser T, Mussgnug JH, Kruse O., J Biotechnol 249(), 2017
PMID: 28302588
High-yield secretion of recombinant proteins from the microalga Chlamydomonas reinhardtii.
Ramos-Martinez EM, Fimognari L, Sakuragi Y., Plant Biotechnol J 15(9), 2017
PMID: 28207991
Compartmentalisation of [FeFe]-hydrogenase maturation in Chlamydomonas reinhardtii.
Sawyer A, Bai Y, Lu Y, Hemschemeier A, Happe T., Plant J 90(6), 2017
PMID: 28295776
A type-I diacylglycerol acyltransferase modulates triacylglycerol biosynthesis and fatty acid composition in the oleaginous microalga, Nannochloropsis oceanica.
Wei H, Shi Y, Ma X, Pan Y, Hu H, Li Y, Luo M, Gerken H, Liu J., Biotechnol Biofuels 10(), 2017
PMID: 28694845
Knock-Down of the IFR1 Protein Perturbs the Homeostasis of Reactive Electrophile Species and Boosts Photosynthetic Hydrogen Production in Chlamydomonas reinhardtii.
Venkanna D, Südfeld C, Baier T, Homburg SV, Patel AV, Wobbe L, Kruse O., Front Plant Sci 8(), 2017
PMID: 28824682
Evolution of rubisco complex small subunit transit peptides from algae to plants.
Razzak MA, Lee DW, Yoo YJ, Hwang I., Sci Rep 7(1), 2017
PMID: 28839179
Efficient expression of nuclear transgenes in the green alga Chlamydomonas: synthesis of an HIV antigen and development of a new selectable marker.
Barahimipour R, Neupert J, Bock R., Plant Mol Biol 90(4-5), 2016
PMID: 26747175
Identification of a Chlamydomonas plastidial 2-lysophosphatidic acid acyltransferase and its use to engineer microalgae with increased oil content.
Yamaoka Y, Achard D, Jang S, Legéret B, Kamisuki S, Ko D, Schulz-Raffelt M, Kim Y, Song WY, Nishida I, Li-Beisson Y, Lee Y., Plant Biotechnol J 14(11), 2016
PMID: 27133096
Calredoxin represents a novel type of calcium-dependent sensor-responder connected to redox regulation in the chloroplast.
Hochmal AK, Zinzius K, Charoenwattanasatien R, Gäbelein P, Mutoh R, Tanaka H, Schulze S, Liu G, Scholz M, Nordhues A, Offenborn JN, Petroutsos D, Finazzi G, Fufezan C, Huang K, Kurisu G, Hippler M., Nat Commun 7(), 2016
PMID: 27297041
Efficient phototrophic production of a high-value sesquiterpenoid from the eukaryotic microalga Chlamydomonas reinhardtii.
Lauersen KJ, Baier T, Wichmann J, Wördenweber R, Mussgnug JH, Hübner W, Huser T, Kruse O., Metab Eng 38(), 2016
PMID: 27474353
Construction of Marker-Free Transgenic Strains of Chlamydomonas reinhardtii Using a Cre/loxP-Mediated Recombinase System.
Kasai Y, Harayama S., PLoS One 11(8), 2016
PMID: 27564988
Chloroplast-mediated regulation of CO2-concentrating mechanism by Ca2+-binding protein CAS in the green alga Chlamydomonas reinhardtii.
Wang L, Yamano T, Takane S, Niikawa Y, Toyokawa C, Ozawa SI, Tokutsu R, Takahashi Y, Minagawa J, Kanesaki Y, Yoshikawa H, Fukuzawa H., Proc Natl Acad Sci U S A 113(44), 2016
PMID: 27791081
UV-mediated Chlamydomonas mutants with enhanced nuclear transgene expression by disruption of DNA methylation-dependent and independent silencing systems.
Kurniasih SD, Yamasaki T, Kong F, Okada S, Widyaningrum D, Ohama T., Plant Mol Biol 92(6), 2016
PMID: 27761764
Molecular techniques to interrogate and edit the Chlamydomonas nuclear genome.
Jinkerson RE, Jonikas MC., Plant J 82(3), 2015
PMID: 25704665
Genetic tools and techniques for Chlamydomonas reinhardtii.
Mussgnug JH., Appl Microbiol Biotechnol 99(13), 2015
PMID: 26025017
Dissecting the contributions of GC content and codon usage to gene expression in the model alga Chlamydomonas reinhardtii.
Barahimipour R, Strenkert D, Neupert J, Schroda M, Merchant SS, Bock R., Plant J 84(4), 2015
PMID: 26402748

52 References

Daten bereitgestellt von Europe PubMed Central.

Stable nuclear transformation of the diatom Phaeodactylum tricornutum.
Apt KE, Kroth-Pancic PG, Grossman AR., Mol. Gen. Genet. 252(5), 1996
PMID: 8914518
Tools for chloroplast transformation in Chlamydomonas: expression vectors and a new dominant selectable marker.
Bateman JM, Purton S., Mol. Gen. Genet. 263(3), 2000
PMID: 10821174
An engineered Streptomyces hygroscopicus aph 7" gene mediates dominant resistance against hygromycin B in Chlamydomonas reinhardtii.
Berthold P, Schmitt R, Mages W., Protist 153(4), 2002
PMID: 12627869
Identification of Monoraphidium contortum as a promising species for liquid biofuel production.
Bogen C, Klassen V, Wichmann J, La Russa M, Doebbe A, Grundmann M, Uronen P, Kruse O, Mussgnug JH., Bioresour. Technol. 133(), 2013
PMID: 23453981
Marker-free genetic engineering of the chloroplast in the green microalga Chlamydomonas reinhardtii.
Chen HC, Melis A., Plant Biotechnol. J. 11(7), 2013
PMID: 23647698
Enhancement of carotenoids biosynthesis in Chlamydomonas reinhardtii by nuclear transformation using a phytoene synthase gene isolated from Chlorella zofingiensis.
Cordero BF, Couso I, Leon R, Rodriguez H, Vargas MA., Appl. Microbiol. Biotechnol. 91(2), 2011
PMID: 21519934
Food commodities from microalgae.
Draaisma RB, Wijffels RH, Slegers PM, Brentner LB, Roy A, Barbosa MJ., Curr. Opin. Biotechnol. 24(2), 2012
PMID: 23084075
Strategies to facilitate transgene expression in Chlamydomonas reinhardtii.
Eichler-Stahlberg A, Weisheit W, Ruecker O, Heitzer M., Planta 229(4), 2009
PMID: 19127370
Preparation of transgenic Dunaliella salina for immunization against white spot syndrome virus in crayfish.
Feng S, Feng W, Zhao L, Gu H, Li Q, Shi K, Guo S, Zhang N., Arch. Virol. 159(3), 2013
PMID: 24081826
Dunaliella salina as a novel host for the production of recombinant proteins.
Feng S, Li X, Xu Z, Qi J., Appl. Microbiol. Biotechnol. 98(10), 2014
PMID: 24643734
The flanking regions of PsaD drive efficient gene expression in the nucleus of the green alga Chlamydomonas reinhardtii.
Fischer N, Rochaix JD., Mol. Genet. Genomics 265(5), 2001
PMID: 11523806
A synthetic gene coding for the green fluorescent protein (GFP) is a versatile reporter in Chlamydomonas reinhardtii.
Fuhrmann M, Oertel W, Hegemann P., Plant J. 19(3), 1999
PMID: 10476082
Monitoring dynamic expression of nuclear genes in Chlamydomonas reinhardtii by using a synthetic luciferase reporter gene.
Fuhrmann M, Hausherr A, Ferbitz L, Schodl T, Heitzer M, Hegemann P., Plant Mol. Biol. 55(6), 2004
PMID: 15604722
Functional hybrid rubisco enzymes with plant small subunits and algal large subunits: engineered rbcS cDNA for expression in chlamydomonas.
Genkov T, Meyer M, Griffiths H, Spreitzer RJ., J. Biol. Chem. 285(26), 2010
PMID: 20424165
A nucleosome assembly protein-like polypeptide binds to chloroplast group II intron RNA in Chlamydomonas reinhardtii.
Glanz S, Bunse A, Wimbert A, Balczun C, Kuck U., Nucleic Acids Res. 34(18), 2006
PMID: 17012281
Raa4 is a trans-splicing factor that specifically binds chloroplast tscA intron RNA.
Glanz S, Jacobs J, Kock V, Mishra A, Kuck U., Plant J. 69(3), 2011
PMID: 21954961
Sequence, evolution and differential expression of the two genes encoding variant small subunits of ribulose bisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii.
Goldschmidt-Clermont M, Rahire M., J. Mol. Biol. 191(3), 1986
PMID: 3820291

A, Transgenic Plant J 1(1), 2007
Visualization of microbodies in Chlamydomonas reinhardtii.
Hayashi Y, Shinozaki A., J. Plant Res. 125(4), 2011
PMID: 22205201
A short amino acid sequence able to specify nuclear location.
Kalderon D, Roberts BL, Richardson WD, Smith AE., Cell 39(3 Pt 2), 1984
PMID: 6096007
High-efficiency homologous recombination in the oil-producing alga Nannochloropsis sp.
Kilian O, Benemann CS, Niyogi KK, Vick B., Proc. Natl. Acad. Sci. U.S.A. 108(52), 2011
PMID: 22123974
High-frequency nuclear transformation of Chlamydomonas reinhardtii.
Kindle KL., Proc. Natl. Acad. Sci. U.S.A. 87(3), 1990
PMID: 2105499
Stable nuclear transformation of Chlamydomonas using the Chlamydomonas gene for nitrate reductase.
Kindle KL, Schnell RA, Fernandez E, Lefebvre PA., J. Cell Biol. 109(6 Pt 1), 1989
PMID: 2592399
Cyan and yellow super fluorescent proteins with improved brightness, protein folding, and FRET Forster radius.
Kremers GJ, Goedhart J, van Munster EB, Gadella TW Jr., Biochemistry 45(21), 2006
PMID: 16716067
Improving FRET dynamic range with bright green and red fluorescent proteins.
Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin MZ., Nat. Methods 9(10), 2012
PMID: 22961245
Efficient recombinant protein production and secretion from nuclear transgenes in Chlamydomonas reinhardtii.
Lauersen KJ, Berger H, Mussgnug JH, Kruse O., J. Biotechnol. 167(2), 2012
PMID: 23099045
Ice recrystallization inhibition mediated by a nuclear-expressed and -secreted recombinant ice-binding protein in the microalga Chlamydomonas reinhardtii.
Lauersen KJ, Vanderveer TL, Berger H, Kaluza I, Mussgnug JH, Walker VK, Kruse O., Appl. Microbiol. Biotechnol. 97(22), 2013
PMID: 24037309
Transgenic microalgae as green cell-factories.
Leon-Banares R, Gonzalez-Ballester D, Galvan A, Fernandez E., Trends Biotechnol. 22(1), 2004
PMID: 14690622
Triacylglycerol profiling of microalgae Chlamydomonas reinhardtii and Nannochloropsis oceanica.
Liu B, Vieler A, Li C, Daniel Jones A, Benning C., Bioresour. Technol. 146(), 2013
PMID: 23948268

V, Plant J 14(4), 1998
An improved cerulean fluorescent protein with enhanced brightness and reduced reversible photoswitching.
Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, Day RN, Wachter RM, Davidson MW, Rizzo MA., PLoS ONE 6(3), 2011
PMID: 21479270
Highly efficient transformation of the diatom Phaeodactylum tricornutum by multi-pulse electroporation.
Miyahara M, Aoi M, Inoue-Kashino N, Kashino Y, Ifuku K., Biosci. Biotechnol. Biochem. 77(4), 2013
PMID: 23563551
Generation of Chlamydomonas strains that efficiently express nuclear transgenes.
Neupert J, Karcher D, Bock R., Plant J. 57(6), 2008
PMID: 19036032
Draft genome sequence and genetic transformation of the oleaginous alga Nannochloropis gaditana.
Radakovits R, Jinkerson RE, Fuerstenberg SI, Tae H, Settlage RE, Boore JL, Posewitz MC., Nat Commun 3(), 2012
PMID: 22353717

BA, Photosynth Res (), 2014
Production of therapeutic proteins in algae, analysis of expression of seven human proteins in the chloroplast of Chlamydomonas reinhardtii.
Rasala BA, Muto M, Lee PA, Jager M, Cardoso RM, Behnke CA, Kirk P, Hokanson CA, Crea R, Mendez M, Mayfield SP., Plant Biotechnol. J. 8(6), 2010
PMID: 20230484
Improved heterologous protein expression in the chloroplast of Chlamydomonas reinhardtii through promoter and 5' untranslated region optimization.
Rasala BA, Muto M, Sullivan J, Mayfield SP., Plant Biotechnol. J. 9(6), 2011
PMID: 21535358
Robust expression and secretion of Xylanase1 in Chlamydomonas reinhardtii by fusion to a selection gene and processing with the FMDV 2A peptide.
Rasala BA, Lee PA, Shen Z, Briggs SP, Mendez M, Mayfield SP., PLoS ONE 7(8), 2012
PMID: 22937037
Expanding the spectral palette of fluorescent proteins for the green microalga Chlamydomonas reinhardtii.
Rasala BA, Barrera DJ, Ng J, Plucinak TM, Rosenberg JN, Weeks DP, Oyler GA, Peterson TC, Haerizadeh F, Mayfield SP., Plant J. 74(4), 2013
PMID: 23521393
Enhanced genetic tools for engineering multigene traits into green algae.
Rasala BA, Chao SS, Pier M, Barrera DJ, Mayfield SP., PLoS ONE 9(4), 2014
PMID: 24710110
High-efficiency biolistic transformation of Chlamydomonas mitochondria can be used to insert mutations in complex I genes.
Remacle C, Cardol P, Coosemans N, Gaisne M, Bonnefoy N., Proc. Natl. Acad. Sci. U.S.A. 103(12), 2006
PMID: 16537419
Chlamydomonas reinhardtii as the photosynthetic yeast.
Rochaix JD., Annu. Rev. Genet. 29(), 1995
PMID: 8825474
Gaussia-luciferase as a sensitive reporter gene for monitoring promoter activity in the nucleus of the green alga Chlamydomonas reinhardtii.
Ruecker O, Zillner K, Groebner-Ferreira R, Heitzer M., Mol. Genet. Genomics 280(2), 2008
PMID: 18516621
The HSP70A promoter as a tool for the improved expression of transgenes in Chlamydomonas.
Schroda M, Blocker D, Beck CF., Plant J. 21(2), 2000
PMID: 10743653
Synthesis of the D2 protein of photosystem II in Chlamydomonas is controlled by a high molecular mass complex containing the RNA stabilization factor Nac2 and the translational activator RBP40.
Schwarz C, Elles I, Kortmann J, Piotrowski M, Nickelsen J., Plant Cell 19(11), 2007
PMID: 18055611
A codon-optimized luciferase from Gaussia princeps facilitates the in vivo monitoring of gene expression in the model alga Chlamydomonas reinhardtii.
Shao N, Bock R., Curr. Genet. 53(6), 2008
PMID: 18408930
Stable nuclear transformation of Chlamydomonas reinhardtii with a Streptomyces rimosus gene as the selective marker.
Sizova IA, Lapina TV, Frolova ON, Alexandrova NN, Akopiants KE, Danilenko VN., Gene 181(1-2), 1996
PMID: 8973302
A Streptomyces rimosus aphVIII gene coding for a new type phosphotransferase provides stable antibiotic resistance to Chlamydomonas reinhardtii.
Sizova I, Fuhrmann M, Hegemann P., Gene 277(1-2), 2001
PMID: 11602359
Future prospects of microalgal biofuel production systems.
Stephens E, Ross IL, Mussgnug JH, Wagner LD, Borowitzka MA, Posten C, Kruse O, Hankamer B., Trends Plant Sci. 15(10), 2010
PMID: 20655798
Recombinant Gaussia luciferase. Overexpression, purification, and analytical application of a bioluminescent reporter for DNA hybridization.
Verhaegent M, Christopoulos TK., Anal. Chem. 74(17), 2002
PMID: 12236345
Genome, functional gene annotation, and nuclear transformation of the heterokont oleaginous alga Nannochloropsis oceanica CCMP1779.
Vieler A, Wu G, Tsai CH, Bullard B, Cornish AJ, Harvey C, Reca IB, Thornburg C, Achawanantakun R, Buehl CJ, Campbell MS, Cavalier D, Childs KL, Clark TJ, Deshpande R, Erickson E, Armenia Ferguson A, Handee W, Kong Q, Li X, Liu B, Lundback S, Peng C, Roston RL, Sanjaya , Simpson JP, Terbush A, Warakanont J, Zauner S, Farre EM, Hegg EL, Jiang N, Kuo MH, Lu Y, Niyogi KK, Ohlrogge J, Osteryoung KW, Shachar-Hill Y, Sears BB, Sun Y, Takahashi H, Yandell M, Shiu SH, Benning C., PLoS Genet. 8(11), 2012
PMID: 23166516
Potential of industrial biotechnology with cyanobacteria and eukaryotic microalgae.
Wijffels RH, Kruse O, Hellingwerf KJ., Curr. Opin. Biotechnol. 24(3), 2013
PMID: 23647970
Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®
Quellen

PMID: 25586579
PubMed | Europe PMC

Suchen in

Google Scholar