Characterization of the pyrophosphate-dependent 6-phosphofructokinase from Xanthomonas campestris pv. campestris

Frese M, Schatschneider S, Voss J, Vorhölter F-J, Niehaus K (2014)
Archives of Biochemistry and Biophysics 546: 53-63.

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Abstract / Bemerkung
Xanthomonads are plant pathogenic proteobacteria that produce the polysaccharide xanthan. They are assumed to catabolize glucose mainly via the Entner-Doudoroff pathway. Whereas previous studies have demonstrated no phosphofructokinase (PFK) activity in xanthomonads, detailed genome analysis revealed in Xanthomonas campestris pathovar campestris (Xcc) genes for all Embden-Meyerhof-Parnas pathway (glycolysis) enzymes, including a conserved pfkA gene similar to 6-phosphofructokinase genes. To address this discrepancy between genetic and physiological properties, the pfkA gene of Xcc strain B100 was cloned into the expression vector pET28a+. The 45-kDa pfkA gene product exhibited no conventional PFK activity. Bioinformatic analysis of the Xcc PfkA amino acid sequence suggested utilization of pyrophosphate as an alternative cosubstrate. Pyrophosphate-dependent PFK activity was shown in an in vitro enzyme assay for purified Xcc PfkA, as well as in the Xcc B100 crude protein extract. Kinetic constants were determined for the forward and reverse reactions. Primary structure conservation indicates the global presence of similar enzymes among Xanthomonadaceae. (C) 2014 Elsevier Inc. All rights reserved.
Two-substrate enzyme; Glucose metabolism; Embden-Meyerhof-Parnas pathway; kinetics; phosphofructokinase; Pyrophosphate-dependent; Glycolysis
Archives of Biochemistry and Biophysics
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Frese M, Schatschneider S, Voss J, Vorhölter F-J, Niehaus K. Characterization of the pyrophosphate-dependent 6-phosphofructokinase from Xanthomonas campestris pv. campestris. Archives of Biochemistry and Biophysics. 2014;546:53-63.
Frese, M., Schatschneider, S., Voss, J., Vorhölter, F. - J., & Niehaus, K. (2014). Characterization of the pyrophosphate-dependent 6-phosphofructokinase from Xanthomonas campestris pv. campestris. Archives of Biochemistry and Biophysics, 546, 53-63. doi:10.1016/
Frese, M., Schatschneider, S., Voss, J., Vorhölter, F. - J., and Niehaus, K. (2014). Characterization of the pyrophosphate-dependent 6-phosphofructokinase from Xanthomonas campestris pv. campestris. Archives of Biochemistry and Biophysics 546, 53-63.
Frese, M., et al., 2014. Characterization of the pyrophosphate-dependent 6-phosphofructokinase from Xanthomonas campestris pv. campestris. Archives of Biochemistry and Biophysics, 546, p 53-63.
M. Frese, et al., “Characterization of the pyrophosphate-dependent 6-phosphofructokinase from Xanthomonas campestris pv. campestris”, Archives of Biochemistry and Biophysics, vol. 546, 2014, pp. 53-63.
Frese, M., Schatschneider, S., Voss, J., Vorhölter, F.-J., Niehaus, K.: Characterization of the pyrophosphate-dependent 6-phosphofructokinase from Xanthomonas campestris pv. campestris. Archives of Biochemistry and Biophysics. 546, 53-63 (2014).
Frese, Marcel, Schatschneider, Sarah, Voss, Julia, Vorhölter, Frank-Jörg, and Niehaus, Karsten. “Characterization of the pyrophosphate-dependent 6-phosphofructokinase from Xanthomonas campestris pv. campestris”. Archives of Biochemistry and Biophysics 546 (2014): 53-63.

4 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Refined annotation of the complete genome of the phytopathogenic and xanthan producing Xanthomonas campestris pv. campestris strain B100 based on RNA sequence data.
Alkhateeb RS, Rückert C, Rupp O, Pucker B, Hublik G, Wibberg D, Niehaus K, Pühler A, Vorhölter FJ., J Biotechnol 253(), 2017
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Schatschneider S, Huber C, Neuweger H, Watt TF, Pühler A, Eisenreich W, Wittmann C, Niehaus K, Vorhölter FJ., Mol Biosyst 10(10), 2014
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