Receptor-Ligand Interactions: Binding Affinities Studied by Single-Molecule and Super-Resolution Microscopy on Intact Cells

Dietz MS, Fricke F, Krueger CL, Niemann H, Heilemann M (2014)
ChemPhysChem 15(4): 671-676.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor/in
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Abstract / Bemerkung
Protein-ligand interactions play an important role in many biological processes. Notably, membrane receptors are the starting point for a huge variety of cellular signal transduction pathways. Quantifying the binding affinity of a ligand for its transmembrane receptor is of great importance as it provides information on the potency of the ligand. We developed a new experimental procedure to determine binding affinities of ligands for their membrane receptors directly on intact single cells using super-resolution imaging. Dissociation constants were determined by titrating fluorophore-labelled ligand against cells expressing the target protein and applying single-molecule imaging.
Stichworte
single-molecule studies; TNF receptor 1; protein-protein interactions; MET receptor; fluorescence microscopy
Erscheinungsjahr
2014
Zeitschriftentitel
ChemPhysChem
Band
15
Ausgabe
4
Seite(n)
671-676
ISSN
1439-4235
Page URI
https://pub.uni-bielefeld.de/record/2673656

Zitieren

Dietz MS, Fricke F, Krueger CL, Niemann H, Heilemann M. Receptor-Ligand Interactions: Binding Affinities Studied by Single-Molecule and Super-Resolution Microscopy on Intact Cells. ChemPhysChem. 2014;15(4):671-676.
Dietz, M. S., Fricke, F., Krueger, C. L., Niemann, H., & Heilemann, M. (2014). Receptor-Ligand Interactions: Binding Affinities Studied by Single-Molecule and Super-Resolution Microscopy on Intact Cells. ChemPhysChem, 15(4), 671-676. doi:10.1002/cphc.201300755
Dietz, M. S., Fricke, F., Krueger, C. L., Niemann, H., and Heilemann, M. (2014). Receptor-Ligand Interactions: Binding Affinities Studied by Single-Molecule and Super-Resolution Microscopy on Intact Cells. ChemPhysChem 15, 671-676.
Dietz, M.S., et al., 2014. Receptor-Ligand Interactions: Binding Affinities Studied by Single-Molecule and Super-Resolution Microscopy on Intact Cells. ChemPhysChem, 15(4), p 671-676.
M.S. Dietz, et al., “Receptor-Ligand Interactions: Binding Affinities Studied by Single-Molecule and Super-Resolution Microscopy on Intact Cells”, ChemPhysChem, vol. 15, 2014, pp. 671-676.
Dietz, M.S., Fricke, F., Krueger, C.L., Niemann, H., Heilemann, M.: Receptor-Ligand Interactions: Binding Affinities Studied by Single-Molecule and Super-Resolution Microscopy on Intact Cells. ChemPhysChem. 15, 671-676 (2014).
Dietz, Marina S., Fricke, Franziska, Krueger, Carmen L., Niemann, Hartmut, and Heilemann, Mike. “Receptor-Ligand Interactions: Binding Affinities Studied by Single-Molecule and Super-Resolution Microscopy on Intact Cells”. ChemPhysChem 15.4 (2014): 671-676.

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