Receptor-Ligand Interactions: Binding Affinities Studied by Single-Molecule and Super-Resolution Microscopy on Intact Cells

Dietz MS, Fricke F, Krueger CL, Niemann H, Heilemann M (2014)
ChemPhysChem 15(4): 671-676.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
DOI
Autor*in
Dietz, Marina S.; Fricke, Franziska; Krueger, Carmen L.; Niemann, HartmutUniBi ; Heilemann, MikeUniBi
Einrichtung
Fakultät für Chemie > Biochemie IV
Abstract / Bemerkung
Protein-ligand interactions play an important role in many biological processes. Notably, membrane receptors are the starting point for a huge variety of cellular signal transduction pathways. Quantifying the binding affinity of a ligand for its transmembrane receptor is of great importance as it provides information on the potency of the ligand. We developed a new experimental procedure to determine binding affinities of ligands for their membrane receptors directly on intact single cells using super-resolution imaging. Dissociation constants were determined by titrating fluorophore-labelled ligand against cells expressing the target protein and applying single-molecule imaging.
Stichworte
single-molecule studies; TNF receptor 1; protein-protein interactions; MET receptor; fluorescence microscopy
Erscheinungsjahr
2014
Zeitschriftentitel
ChemPhysChem
Band
15
Ausgabe
4
Seite(n)
671-676
ISSN
1439-4235
Page URI
https://pub.uni-bielefeld.de/record/2673656

Zitieren

Dietz MS, Fricke F, Krueger CL, Niemann H, Heilemann M. Receptor-Ligand Interactions: Binding Affinities Studied by Single-Molecule and Super-Resolution Microscopy on Intact Cells. ChemPhysChem. 2014;15(4):671-676.
Dietz, M. S., Fricke, F., Krueger, C. L., Niemann, H., & Heilemann, M. (2014). Receptor-Ligand Interactions: Binding Affinities Studied by Single-Molecule and Super-Resolution Microscopy on Intact Cells. ChemPhysChem, 15(4), 671-676. doi:10.1002/cphc.201300755
Dietz, Marina S., Fricke, Franziska, Krueger, Carmen L., Niemann, Hartmut, and Heilemann, Mike. 2014. “Receptor-Ligand Interactions: Binding Affinities Studied by Single-Molecule and Super-Resolution Microscopy on Intact Cells”. ChemPhysChem 15 (4): 671-676.
Dietz, M. S., Fricke, F., Krueger, C. L., Niemann, H., and Heilemann, M. (2014). Receptor-Ligand Interactions: Binding Affinities Studied by Single-Molecule and Super-Resolution Microscopy on Intact Cells. ChemPhysChem 15, 671-676.
Dietz, M.S., et al., 2014. Receptor-Ligand Interactions: Binding Affinities Studied by Single-Molecule and Super-Resolution Microscopy on Intact Cells. ChemPhysChem, 15(4), p 671-676.
M.S. Dietz, et al., “Receptor-Ligand Interactions: Binding Affinities Studied by Single-Molecule and Super-Resolution Microscopy on Intact Cells”, ChemPhysChem, vol. 15, 2014, pp. 671-676.
Dietz, M.S., Fricke, F., Krueger, C.L., Niemann, H., Heilemann, M.: Receptor-Ligand Interactions: Binding Affinities Studied by Single-Molecule and Super-Resolution Microscopy on Intact Cells. ChemPhysChem. 15, 671-676 (2014).
Dietz, Marina S., Fricke, Franziska, Krueger, Carmen L., Niemann, Hartmut, and Heilemann, Mike. “Receptor-Ligand Interactions: Binding Affinities Studied by Single-Molecule and Super-Resolution Microscopy on Intact Cells”. ChemPhysChem 15.4 (2014): 671-676.

15 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Model-based identification of TNFα-induced IKKβ-mediated and IκBα-mediated regulation of NFκB signal transduction as a tool to quantify the impact of drug-induced liver injury compounds.
Oppelt A, Kaschek D, Huppelschoten S, Sison-Young R, Zhang F, Buck-Wiese M, Herrmann F, Malkusch S, Krüger CL, Meub M, Merkt B, Zimmermann L, Schofield A, Jones RP, Malik H, Schilling M, Heilemann M, van de Water B, Goldring CE, Park BK, Timmer J, Klingmüller U., NPJ Syst Biol Appl 4(), 2018
PMID: 29900006
A Platform To Enhance Quantitative Single Molecule Localization Microscopy.
Golfetto O, Wakefield DL, Cacao EE, Avery KN, Kenyon V, Jorand R, Tobin SJ, Biswas S, Gutierrez J, Clinton R, Ma Y, Horne DA, Williams JC, Jovanović-Talisman T., J Am Chem Soc 140(40), 2018
PMID: 30256630
Quantitative and integrative analysis of paracrine hepatocyte activation by nonparenchymal cells upon lipopolysaccharide induction.
Beuke K, Schildberg FA, Pinna F, Albrecht U, Liebe R, Bissinger M, Schirmacher P, Dooley S, Bode JG, Knolle PA, Kummer U, Breuhahn K, Sahle S., FEBS J 284(5), 2017
PMID: 28109179
Linear ubiquitination of cytosolic Salmonella Typhimurium activates NF-κB and restricts bacterial proliferation.
van Wijk SJL, Fricke F, Herhaus L, Gupta J, Hötte K, Pampaloni F, Grumati P, Kaulich M, Sou YS, Komatsu M, Greten FR, Fulda S, Heilemann M, Dikic I., Nat Microbiol 2(), 2017
PMID: 28481361
Imaging of anticancer drug action in single cells.
Miller MA, Weissleder R., Nat Rev Cancer 17(7), 2017
PMID: 28642603
Expanding the host cell ubiquitylation machinery targeting cytosolic Salmonella.
Polajnar M, Dietz MS, Heilemann M, Behrends C., EMBO Rep 18(9), 2017
PMID: 28784601
Evaluation of peptide designing strategy against subunit reassociation in mucin 1: A steered molecular dynamics approach.
Lesitha Jeeva Kumari J, Jesu Jaya Sudan R, Sudandiradoss C., PLoS One 12(8), 2017
PMID: 28817726
Transparent Substrates Prepared From Different Amorphous Polymers Can Directly Modulate Primary Human B cell functions.
Roch T, Hahne S, Kratz K, Ma N, Lendlein A., Biotechnol J 12(12), 2017
PMID: 28857458
PCNA appears in two populations of slow and fast diffusion with a constant ratio throughout S-phase in replicating mammalian cells.
Zessin PJ, Sporbert A, Heilemann M., Sci Rep 6(), 2016
PMID: 26758689
Live-cell protein labelling with nanometre precision by cell squeezing.
Kollmannsperger A, Sharei A, Raulf A, Heilemann M, Langer R, Jensen KF, Wieneke R, Tampé R., Nat Commun 7(), 2016
PMID: 26822409
Selective Detection of RGD-Integrin Binding in Cancer Cells Using Tip Enhanced Raman Scattering Microscopy.
Xiao L, Wang H, Schultz ZD., Anal Chem 88(12), 2016
PMID: 27189228
Single-molecule methods to study membrane receptor oligomerization.
Fricke F, Dietz MS, Heilemann M., Chemphyschem 16(4), 2015
PMID: 25521567
Analysis of Interactions between the Epidermal Growth Factor Receptor and Soluble Ligands on the Basis of Single-Molecule Diffusivity in the Membrane of Living Cells.
Kim DH, Zhou K, Kim DK, Park S, Noh J, Kwon Y, Kim D, Song NW, Lee JB, Suh PG, Lee NK, Ryu SH., Angew Chem Int Ed Engl 54(24), 2015
PMID: 25940988
Quantitative single-molecule localization microscopy combined with rule-based modeling reveals ligand-induced TNF-R1 reorganization toward higher-order oligomers.
Fricke F, Malkusch S, Wangorsch G, Greiner JF, Kaltschmidt B, Kaltschmidt C, Widera D, Dandekar T, Heilemann M., Histochem Cell Biol 142(1), 2014
PMID: 24519400
Quantitative analysis of single-molecule superresolution images.
Coltharp C, Yang X, Xiao J., Curr Opin Struct Biol 28(), 2014
PMID: 25179006

52 References

Daten bereitgestellt von Europe PubMed Central.

Discrimination between intact cell desensitization and agonist affinity changes.
Barber R., Mol. Cell. Endocrinol. 46(3), 1986
PMID: 3017784
Estimation of the kinetic constants for binding of epinephrine to beta-adrenergic receptors of the S49 cell.
Stickle D, Barber R., Biochem. Pharmacol. 42(5), 1991
PMID: 1651730
AVIDIN. 1. THE USE OF (14-C)BIOTIN FOR KINETIC STUDIES AND FOR ASSAY.
GREEN NM., Biochem. J. 89(), 1963
PMID: 14101979
Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G.
Engvall E, Perlmann P., Immunochemistry 8(9), 1971
PMID: 5135623
Immunoassay using antigen-enzyme conjugates.
Van Weemen BK, Schuurs AH., FEBS Lett. 15(3), 1971
PMID: 11945853

Liedberg, Sens. Actuators 4(), 1983
Radioreceptor assay of adrenocorticotropic hormone: new approach to assay of polypeptide hormones in plasma.
Lefkowitz RJ, Roth J, Pastan I., Science 170(3958), 1970
PMID: 4319388
Thermodynamics of protein-ligand interactions: history, presence, and future aspects.
Perozzo R, Folkers G, Scapozza L., J. Recept. Signal Transduct. Res. 24(1-2), 2004
PMID: 15344878
Fluorescence correlation spectroscopy. II. An experimental realization.
Magde D, Elson EL, Webb WW., Biopolymers 13(1), 1974
PMID: 4818131
Ultrasensitive hybridization analysis using fluorescence correlation spectroscopy.
Kinjo M, Rigler R., Nucleic Acids Res. 23(10), 1995
PMID: 7784185
Fluorescence correlation spectroscopy with single-molecule sensitivity on cell and model membranes.
Schwille P, Korlach J, Webb WW., Cytometry 36(3), 1999
PMID: 10404965

Baaske, Angew. Chem. 122(), 2010
Optical thermophoresis for quantifying the buffer dependence of aptamer binding.
Baaske P, Wienken CJ, Reineck P, Duhr S, Braun D., Angew. Chem. Int. Ed. Engl. 49(12), 2010
PMID: 20186894
Fluorescence microscopy beyond the diffraction limit.
Heilemann M., J. Biotechnol. 149(4), 2010
PMID: 20347891
Imaging intracellular fluorescent proteins at nanometer resolution.
Betzig E, Patterson GH, Sougrat R, Lindwasser OW, Olenych S, Bonifacino JS, Davidson MW, Lippincott-Schwartz J, Hess HF., Science 313(5793), 2006
PMID: 16902090
Ultra-high resolution imaging by fluorescence photoactivation localization microscopy.
Hess ST, Girirajan TP, Mason MD., Biophys. J. 91(11), 2006
PMID: 16980368
Multicolor super-resolution imaging with photo-switchable fluorescent probes.
Bates M, Huang B, Dempsey GT, Zhuang X., Science 317(5845), 2007
PMID: 17702910

Heilemann, Angew. Chem. 120(), 2008
Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes.
Heilemann M, van de Linde S, Schuttpelz M, Kasper R, Seefeldt B, Mukherjee A, Tinnefeld P, Sauer M., Angew. Chem. Int. Ed. Engl. 47(33), 2008
PMID: 18646237
Visualizing cell structure and function with point-localization superresolution imaging.
Sengupta P, Van Engelenburg S, Lippincott-Schwartz J., Dev. Cell 23(6), 2012
PMID: 23237943
Met, metastasis, motility and more.
Birchmeier C, Birchmeier W, Gherardi E, Vande Woude GF., Nat. Rev. Mol. Cell Biol. 4(12), 2003
PMID: 14685170
InIB-dependent internalization of Listeria is mediated by the Met receptor tyrosine kinase.
Shen Y, Naujokas M, Park M, Ireton K., Cell 103(3), 2000
PMID: 11081636
Life and death by death receptors.
Guicciardi ME, Gores GJ., FASEB J. 23(6), 2009
PMID: 19141537
Cell density-dependent regulation of hepatocyte growth factor receptor on adult rat hepatocytes in primary culture.
Mizuno K, Higuchi O, Tajima H, Yonemasu T, Nakamura T., J. Biochem. 114(1), 1993
PMID: 8407884
Characterization of hepatocyte-growth-factor receptors on Meth A cells.
Komada M, Miyazawa K, Ishii T, Kitamura N., Eur. J. Biochem. 204(2), 1992
PMID: 1311683
Cooperative interaction between alpha- and beta-chains of hepatocyte growth factor on c-Met receptor confers ligand-induced receptor tyrosine phosphorylation and multiple biological responses.
Matsumoto K, Kataoka H, Date K, Nakamura T., J. Biol. Chem. 273(36), 1998
PMID: 9722511
Synergistic differentiation-promoting activity of interferon gamma and tumor necrosis factor-alpha: role of receptor regulation on human neuroblasts.
Montaldo PG, Carbone R, Corrias MV, Ferraris PC, Ponzoni M., J. Natl. Cancer Inst. 86(22), 1994
PMID: 7966397
Tumor necrosis factor: specific binding and internalization in sensitive and resistant cells.
Tsujimoto M, Yip YK, Vilcek J., Proc. Natl. Acad. Sci. U.S.A. 82(22), 1985
PMID: 2999773
Single-molecule photobleaching reveals increased MET receptor dimerization upon ligand binding in intact cells.
Dietz MS, Haße D, Ferraris DM, Gohler A, Niemann HH, Heilemann M., BMC Biophys 6(1), 2013
PMID: 23731667
TNFR1 signaling is associated with backbone conformational changes of receptor dimers consistent with overactivation in the R92Q TRAPS mutant.
Lewis AK, Valley CC, Sachs JN., Biochemistry 51(33), 2012
PMID: 22799488
Single-molecule analysis of epidermal growth factor binding on the surface of living cells.
Teramura Y, Ichinose J, Takagi H, Nishida K, Yanagida T, Sako Y., EMBO J. 25(18), 2006
PMID: 16946702
Counting single photoactivatable fluorescent molecules by photoactivated localization microscopy (PALM).
Lee SH, Shin JY, Lee A, Bustamante C., Proc. Natl. Acad. Sci. U.S.A. 109(43), 2012
PMID: 23045631
Quantitative single-molecule microscopy reveals that CENP-A(Cnp1) deposition occurs during G2 in fission yeast.
Lando D, Endesfelder U, Berger H, Subramanian L, Dunne PD, McColl J, Klenerman D, Carr AM, Sauer M, Allshire RC, Heilemann M, Laue ED., Open Biol 2(7), 2012
PMID: 22870388
Probing protein heterogeneity in the plasma membrane using PALM and pair correlation analysis.
Sengupta P, Jovanovic-Talisman T, Skoko D, Renz M, Veatch SL, Lippincott-Schwartz J., Nat. Methods 8(11), 2011
PMID: 21926998
Identification of a 60-kD tumor necrosis factor (TNF) receptor as the major signal transducing component in TNF responses.
Thoma B, Grell M, Pfizenmaier K, Scheurich P., J. Exp. Med. 172(4), 1990
PMID: 2170559
Identification of two types of tumor necrosis factor receptors on human cell lines by monoclonal antibodies.
Brockhaus M, Schoenfeld HJ, Schlaeger EJ, Hunziker W, Lesslauer W, Loetscher H., Proc. Natl. Acad. Sci. U.S.A. 87(8), 1990
PMID: 2158104
Structure of the human receptor tyrosine kinase met in complex with the Listeria invasion protein InlB.
Niemann HH, Jager V, Butler PJ, van den Heuvel J, Schmidt S, Ferraris D, Gherardi E, Heinz DW., Cell 130(2), 2007
PMID: 17662939
Ligand-mediated dimerization of the Met receptor tyrosine kinase by the bacterial invasion protein InlB.
Ferraris DM, Gherardi E, Di Y, Heinz DW, Niemann HH., J. Mol. Biol. 395(3), 2009
PMID: 19900460
The structure of tumor necrosis factor-alpha at 2.6 A resolution. Implications for receptor binding.
Eck MJ, Sprang SR., J. Biol. Chem. 264(29), 1989
PMID: 2551905
Crystal structure of the soluble human 55 kd TNF receptor-human TNF beta complex: implications for TNF receptor activation.
Banner DW, D'Arcy A, Janes W, Gentz R, Schoenfeld HJ, Broger C, Loetscher H, Lesslauer W., Cell 73(3), 1993
PMID: 8387891
Aromatic amino acids at the surface of InlB are essential for host cell invasion by Listeria monocytogenes.
Machner MP, Frese S, Schubert WD, Orian-Rousseau V, Gherardi E, Wehland J, Niemann HH, Heinz DW., Mol. Microbiol. 48(6), 2003
PMID: 12791136
Fold and function of the InlB B-repeat.
Ebbes M, Bleymuller WM, Cernescu M, Nolker R, Brutschy B, Niemann HH., J. Biol. Chem. 286(17), 2011
PMID: 21345802
Engineered variants of InlB with an additional leucine-rich repeat discriminate between physiologically relevant and packing contacts in crystal structures of the InlB:MET complex.
Niemann HH, Gherardi E, Bleymuller WM, Heinz DW., Protein Sci. 21(10), 2012
PMID: 22887347
TNF ligands and receptors--a matter of life and death.
MacEwan DJ., Br. J. Pharmacol. 135(4), 2002
PMID: 11861313
Cellular receptor for 125I-labeled tumor necrosis factor: specific binding, affinity labeling, and relationship to sensitivity.
Kull FC Jr, Jacobs S, Cuatrecasas P., Proc. Natl. Acad. Sci. U.S.A. 82(17), 1985
PMID: 2994048
Human TNF mutants with selective activity on the p55 receptor.
Van Ostade X, Vandenabeele P, Everaerdt B, Loetscher H, Gentz R, Brockhaus M, Lesslauer W, Tavernier J, Brouckaert P, Fiers W., Nature 361(6409), 1993
PMID: 8380906
Binding efficiency of protein-protein complexes.
Day ES, Cote SM, Whitty A., Biochemistry 51(45), 2012
PMID: 23088250
A soluble 17 kDa tumour necrosis factor (TNF) mutein, TNF-SAM2, with membrane-bound TNF-like biological characteristics.
Yoshida A, Kohchi C, Inagawa H, Nishizawa T, Hori H, Soma G., Anticancer Res. 26(6A), 2006
PMID: 17195449
Tumor necrosis factor (TNF) alpha quantification by ELISA and bioassay: effects of TNF alpha-soluble TNF receptor (p55) complex dissociation during assay incubations.
Corti A, Poiesi C, Merli S, Cassani G., J. Immunol. Methods 177(1-2), 1994
PMID: 7822826
Three is better than one: pre-ligand receptor assembly in the regulation of TNF receptor signaling.
Chan FK., Cytokine 37(2), 2007
PMID: 17449269
Real-time computation of subdiffraction-resolution fluorescence images.
Wolter S, Schuttpelz M, Tscherepanow M, VAN DE Linde S, Heilemann M, Sauer M., J Microsc 237(1), 2010
PMID: 20055915
A guided tour into subcellular colocalization analysis in light microscopy.
Bolte S, Cordelieres FP., J Microsc 224(Pt 3), 2006
PMID: 17210054
Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®
Quellen

PMID: 24772464
PubMed | Europe PMC

Suchen in

Google Scholar