Genome rearrangements of Streptomyces albus J1074 lead to the carotenoid gene cluster activation

Myronovskyi M, Tokovenko B, Brötz E, Rückert C, Kalinowski J, Luzhetskyy A (2014)
Applied microbiology and biotechnology 98(2): 795-806.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Autor/in
; ; ; ; ;
Abstract / Bemerkung
Streptomyces albus J1074 is a derivative of the S. albus G1 strain defective in SalG1 restriction-modification system. Genome sequencing of S. albus J1074 revealed that the size of its chromosome is 6.8 Mb with unusually short terminal arms of only 0.3 and 0.4 Mb. Here we present our attempts to evaluate the dispensability of subtelomeric regions of the S. albus J1074 chromosome. A number of large site-directed genomic deletions led to circularization of the S. albus J1074 chromosome and to the overall genome reduction by 307 kb. Two spontaneous mutants with an activated carotenoid cluster were obtained. Genome sequencing and transcriptome analysis indicated that phenotypes of these mutants resulted from the right terminal 0.42 Mb chromosomal region deletion, followed by the carotenoid cluster amplification. Our results indicate that the right terminal 0.42 Mb fragment is dispensable under laboratory conditions. In contrast, the left terminal arm of the S. albus J1074 chromosome contains essential genes and only 42 kb terminal region is proved to be dispensable. We identified overexpressed carotenoid compounds and determined fitness costs of the large genomic rearrangements.
Erscheinungsjahr
2014
Zeitschriftentitel
Applied microbiology and biotechnology
Band
98
Ausgabe
2
Seite(n)
795-806
ISSN
0175-7598
eISSN
1432-0614
Page URI
https://pub.uni-bielefeld.de/record/2662843

Zitieren

Myronovskyi M, Tokovenko B, Brötz E, Rückert C, Kalinowski J, Luzhetskyy A. Genome rearrangements of Streptomyces albus J1074 lead to the carotenoid gene cluster activation. Applied microbiology and biotechnology. 2014;98(2):795-806.
Myronovskyi, M., Tokovenko, B., Brötz, E., Rückert, C., Kalinowski, J., & Luzhetskyy, A. (2014). Genome rearrangements of Streptomyces albus J1074 lead to the carotenoid gene cluster activation. Applied microbiology and biotechnology, 98(2), 795-806. doi:10.1007/s00253-013-5440-6
Myronovskyi, M., Tokovenko, B., Brötz, E., Rückert, C., Kalinowski, J., and Luzhetskyy, A. (2014). Genome rearrangements of Streptomyces albus J1074 lead to the carotenoid gene cluster activation. Applied microbiology and biotechnology 98, 795-806.
Myronovskyi, M., et al., 2014. Genome rearrangements of Streptomyces albus J1074 lead to the carotenoid gene cluster activation. Applied microbiology and biotechnology, 98(2), p 795-806.
M. Myronovskyi, et al., “Genome rearrangements of Streptomyces albus J1074 lead to the carotenoid gene cluster activation”, Applied microbiology and biotechnology, vol. 98, 2014, pp. 795-806.
Myronovskyi, M., Tokovenko, B., Brötz, E., Rückert, C., Kalinowski, J., Luzhetskyy, A.: Genome rearrangements of Streptomyces albus J1074 lead to the carotenoid gene cluster activation. Applied microbiology and biotechnology. 98, 795-806 (2014).
Myronovskyi, Maksym, Tokovenko, Bogdan, Brötz, Elke, Rückert, Christian, Kalinowski, Jörn, and Luzhetskyy, Andriy. “Genome rearrangements of Streptomyces albus J1074 lead to the carotenoid gene cluster activation”. Applied microbiology and biotechnology 98.2 (2014): 795-806.

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 24337397
PubMed | Europe PMC

Suchen in

Google Scholar