Quantification of Förster resonance energy transfer by monitoring sensitized emission in living plant cells
Müller S, Galliardt H, Schneider J, Barisas BG, Seidel T (2013)
Frontiers in Plant Science 4: 413.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
Download
Autor*in
Müller, SaraUniBi;
Galliardt, HelenaUniBi;
Schneider, JessicaUniBi;
Barisas, B. George;
Seidel, ThorstenUniBi
Abstract / Bemerkung
Förster resonance energy transfer (FRET) describes excitation energy exchange between two adjacent molecules typically in distances ranging from 2 to 10 nm. The process depends on dipole-dipole coupling of the molecules and its probability of occurrence cannot be proven directly. Mostly, fluorescence is employed for quantification as it represents a concurring process of relaxation of the excited singlet state S1 so that the probability of fluorescence decreases as the probability of FRET increases. This reflects closer proximity of the molecules or an orientation of donor and acceptor transition dipoles that facilitates FRET. Monitoring sensitized emission by 3-Filter-FRET allows for fast image acquisition and is suitable for quantifying FRET in dynamic systems such as living cells. In recent years, several calibration protocols were established to overcome to previous difficulties in measuring FRET-efficiencies. Thus, we can now obtain by 3-filter FRET FRET-efficiencies that are comparable to results from sophisticated fluorescence lifetime measurements. With the discovery of fluorescent proteins and their improvement toward spectral variants and usability in plant cells, the tool box for in vivo FRET-analyses in plant cells was provided and FRET became applicable for the in vivo detection of protein-protein interactions and for monitoring conformational dynamics. The latter opened the door toward a multitude of FRET-sensors such as the widely applied Ca(2+)-sensor Cameleon. Recently, FRET-couples of two fluorescent proteins were supplemented by additional fluorescent proteins toward FRET-cascades in order to monitor more complex arrangements. Novel FRET-couples involving switchable fluorescent proteins promise to increase the utility of FRET through combination with photoactivation-based super-resolution microscopy.
Erscheinungsjahr
2013
Zeitschriftentitel
Frontiers in Plant Science
Band
4
Art.-Nr.
413
ISSN
1664-462X
Page URI
https://pub.uni-bielefeld.de/record/2634390
Zitieren
Müller S, Galliardt H, Schneider J, Barisas BG, Seidel T. Quantification of Förster resonance energy transfer by monitoring sensitized emission in living plant cells. Frontiers in Plant Science. 2013;4: 413.
Müller, S., Galliardt, H., Schneider, J., Barisas, B. G., & Seidel, T. (2013). Quantification of Förster resonance energy transfer by monitoring sensitized emission in living plant cells. Frontiers in Plant Science, 4, 413. doi:10.3389/fpls.2013.00413
Müller, Sara, Galliardt, Helena, Schneider, Jessica, Barisas, B. George, and Seidel, Thorsten. 2013. “Quantification of Förster resonance energy transfer by monitoring sensitized emission in living plant cells”. Frontiers in Plant Science 4: 413.
Müller, S., Galliardt, H., Schneider, J., Barisas, B. G., and Seidel, T. (2013). Quantification of Förster resonance energy transfer by monitoring sensitized emission in living plant cells. Frontiers in Plant Science 4:413.
Müller, S., et al., 2013. Quantification of Förster resonance energy transfer by monitoring sensitized emission in living plant cells. Frontiers in Plant Science, 4: 413.
S. Müller, et al., “Quantification of Förster resonance energy transfer by monitoring sensitized emission in living plant cells”, Frontiers in Plant Science, vol. 4, 2013, : 413.
Müller, S., Galliardt, H., Schneider, J., Barisas, B.G., Seidel, T.: Quantification of Förster resonance energy transfer by monitoring sensitized emission in living plant cells. Frontiers in Plant Science. 4, : 413 (2013).
Müller, Sara, Galliardt, Helena, Schneider, Jessica, Barisas, B. George, and Seidel, Thorsten. “Quantification of Förster resonance energy transfer by monitoring sensitized emission in living plant cells”. Frontiers in Plant Science 4 (2013): 413.
Alle Dateien verfügbar unter der/den folgenden Lizenz(en):
Copyright Statement:
Dieses Objekt ist durch das Urheberrecht und/oder verwandte Schutzrechte geschützt. [...]
Volltext(e)
Access Level
Open Access
Zuletzt Hochgeladen
2019-09-06T09:18:19Z
MD5 Prüfsumme
dc114daa7b682607f29e406581b436de
Daten bereitgestellt von European Bioinformatics Institute (EBI)
23 Zitationen in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
Oxygen Sensing, Hypoxia Tracing and in Vivo Imaging with Functional Metalloprobes for the Early Detection of Non-communicable Diseases.
Mirabello V, Cortezon-Tamarit F, Pascu SI., Front Chem 6(), 2018
PMID: 29527524
Mirabello V, Cortezon-Tamarit F, Pascu SI., Front Chem 6(), 2018
PMID: 29527524
Exploring the Spatiotemporal Organization of Membrane Proteins in Living Plant Cells.
Wang L, Xue Y, Xing J, Song K, Lin J., Annu Rev Plant Biol 69(), 2018
PMID: 29489393
Wang L, Xue Y, Xing J, Song K, Lin J., Annu Rev Plant Biol 69(), 2018
PMID: 29489393
Targeting Phosphopeptide Recognition by the Human BRCA1 Tandem BRCT Domain to Interrupt BRCA1-Dependent Signaling.
Periasamy J, Kurdekar V, Jasti S, Nijaguna MB, Boggaram S, Hurakadli MA, Raina D, Kurup LM, Chintha C, Manjunath K, Goyal A, Sadasivam G, Bharatham K, Padigaru M, Potluri V, Venkitaraman AR., Cell Chem Biol 25(6), 2018
PMID: 29606576
Periasamy J, Kurdekar V, Jasti S, Nijaguna MB, Boggaram S, Hurakadli MA, Raina D, Kurup LM, Chintha C, Manjunath K, Goyal A, Sadasivam G, Bharatham K, Padigaru M, Potluri V, Venkitaraman AR., Cell Chem Biol 25(6), 2018
PMID: 29606576
Direct multiplex imaging and optogenetics of Rho GTPases enabled by near-infrared FRET.
Shcherbakova DM, Cox Cammer N, Huisman TM, Verkhusha VV, Hodgson L., Nat Chem Biol 14(6), 2018
PMID: 29686359
Shcherbakova DM, Cox Cammer N, Huisman TM, Verkhusha VV, Hodgson L., Nat Chem Biol 14(6), 2018
PMID: 29686359
Near-Infrared Fluorescent Proteins: Multiplexing and Optogenetics across Scales.
Shcherbakova DM, Stepanenko OV, Turoverov KK, Verkhusha VV., Trends Biotechnol 36(12), 2018
PMID: 30041828
Shcherbakova DM, Stepanenko OV, Turoverov KK, Verkhusha VV., Trends Biotechnol 36(12), 2018
PMID: 30041828
ePro-ClearSee: a simple immunohistochemical method that does not require sectioning of plant samples.
Nagaki K, Yamaji N, Murata M., Sci Rep 7(), 2017
PMID: 28176832
Nagaki K, Yamaji N, Murata M., Sci Rep 7(), 2017
PMID: 28176832
Conformational changes in inhibitory PAS domain protein associated with binding of HIF-1α and Bcl-xL in living cells.
Kasai S, Kajimoto S, Ito Y, Saito T, Yasumoto KI, Tokunaga M, Sakata-Sogawa K, Fukumura H, Sogawa K., J Biochem 161(3), 2017
PMID: 28003430
Kasai S, Kajimoto S, Ito Y, Saito T, Yasumoto KI, Tokunaga M, Sakata-Sogawa K, Fukumura H, Sogawa K., J Biochem 161(3), 2017
PMID: 28003430
PII Protein-Derived FRET Sensors for Quantification and Live-Cell Imaging of 2-Oxoglutarate.
Lüddecke J, Francois L, Spät P, Watzer B, Chilczuk T, Poschet G, Hell R, Radlwimmer B, Forchhammer K., Sci Rep 7(1), 2017
PMID: 28469248
Lüddecke J, Francois L, Spät P, Watzer B, Chilczuk T, Poschet G, Hell R, Radlwimmer B, Forchhammer K., Sci Rep 7(1), 2017
PMID: 28469248
Quantitatively Understanding Plant Signaling: Novel Theoretical-Experimental Approaches.
Samodelov SL, Zurbriggen MD., Trends Plant Sci 22(8), 2017
PMID: 28668509
Samodelov SL, Zurbriggen MD., Trends Plant Sci 22(8), 2017
PMID: 28668509
FRET-Mediated Long-Range Wavelength Transformation by Photoconvertible Fluorescent Proteins as an Efficient Mechanism to Generate Orange-Red Light in Symbiotic Deep Water Corals.
Bollati E, Plimmer D, D'Angelo C, Wiedenmann J., Int J Mol Sci 18(7), 2017
PMID: 28677653
Bollati E, Plimmer D, D'Angelo C, Wiedenmann J., Int J Mol Sci 18(7), 2017
PMID: 28677653
Rapid Estimation of Astaxanthin and the Carotenoid-to-Chlorophyll Ratio in the Green Microalga Chromochloris zofingiensis Using Flow Cytometry.
Chen J, Wei D, Pohnert G., Mar Drugs 15(7), 2017
PMID: 28753934
Chen J, Wei D, Pohnert G., Mar Drugs 15(7), 2017
PMID: 28753934
Conserved redox-dependent DNA binding of ROXY glutaredoxins with TGA transcription factors.
Gutsche N, Holtmannspötter M, Maß L, O'Donoghue M, Busch A, Lauri A, Schubert V, Zachgo S., Plant Direct 1(6), 2017
PMID: 31245678
Gutsche N, Holtmannspötter M, Maß L, O'Donoghue M, Busch A, Lauri A, Schubert V, Zachgo S., Plant Direct 1(6), 2017
PMID: 31245678
Resonance energy transfer and competing processes in donor-acceptor of sodium zinc (II)-2,9,16,23-phthalocyanine tetracarboxylate molecule.
Al-Omari S., J Biol Phys 42(3), 2016
PMID: 27008342
Al-Omari S., J Biol Phys 42(3), 2016
PMID: 27008342
Techniques for the Analysis of Protein-Protein Interactions in Vivo.
Xing S, Wallmeroth N, Berendzen KW, Grefen C., Plant Physiol 171(2), 2016
PMID: 27208310
Xing S, Wallmeroth N, Berendzen KW, Grefen C., Plant Physiol 171(2), 2016
PMID: 27208310
NUCLEAR FACTOR Y, Subunit C (NF-YC) Transcription Factors Are Positive Regulators of Photomorphogenesis in Arabidopsis thaliana.
Myers ZA, Kumimoto RW, Siriwardana CL, Gayler KK, Risinger JR, Pezzetta D, Holt Iii BF., PLoS Genet 12(9), 2016
PMID: 27685091
Myers ZA, Kumimoto RW, Siriwardana CL, Gayler KK, Risinger JR, Pezzetta D, Holt Iii BF., PLoS Genet 12(9), 2016
PMID: 27685091
Two-photon imaging with longer wavelength excitation in intact Arabidopsis tissues.
Mizuta Y, Kurihara D, Higashiyama T., Protoplasma 252(5), 2015
PMID: 25588923
Mizuta Y, Kurihara D, Higashiyama T., Protoplasma 252(5), 2015
PMID: 25588923
Understanding FRET as a research tool for cellular studies.
Shrestha D, Jenei A, Nagy P, Vereb G, Szöllősi J., Int J Mol Sci 16(4), 2015
PMID: 25815593
Shrestha D, Jenei A, Nagy P, Vereb G, Szöllősi J., Int J Mol Sci 16(4), 2015
PMID: 25815593
Transient plant transformation mediated by Agrobacterium tumefaciens: Principles, methods and applications.
Krenek P, Samajova O, Luptovciak I, Doskocilova A, Komis G, Samaj J., Biotechnol Adv 33(6 pt 2), 2015
PMID: 25819757
Krenek P, Samajova O, Luptovciak I, Doskocilova A, Komis G, Samaj J., Biotechnol Adv 33(6 pt 2), 2015
PMID: 25819757
Binary 2in1 Vectors Improve in Planta (Co)localization and Dynamic Protein Interaction Studies.
Hecker A, Wallmeroth N, Peter S, Blatt MR, Harter K, Grefen C., Plant Physiol 168(3), 2015
PMID: 25971551
Hecker A, Wallmeroth N, Peter S, Blatt MR, Harter K, Grefen C., Plant Physiol 168(3), 2015
PMID: 25971551
Fluorescent proteins as genetically encoded FRET biosensors in life sciences.
Hochreiter B, Garcia AP, Schmid JA., Sensors (Basel) 15(10), 2015
PMID: 26501285
Hochreiter B, Garcia AP, Schmid JA., Sensors (Basel) 15(10), 2015
PMID: 26501285
ClearSee: a rapid optical clearing reagent for whole-plant fluorescence imaging.
Kurihara D, Mizuta Y, Sato Y, Higashiyama T., Development 142(23), 2015
PMID: 26493404
Kurihara D, Mizuta Y, Sato Y, Higashiyama T., Development 142(23), 2015
PMID: 26493404
Functional imaging in living plants-cell biology meets physiology.
Littlejohn GR, Meckel T, Schwarzländer M, Costa A., Front Plant Sci 5(), 2014
PMID: 25566307
Littlejohn GR, Meckel T, Schwarzländer M, Costa A., Front Plant Sci 5(), 2014
PMID: 25566307
Noninvasive high-throughput single-cell analysis of HIV protease activity using ratiometric flow cytometry.
Gaber R, Majerle A, Jerala R, Benčina M., Sensors (Basel) 13(12), 2013
PMID: 24287545
Gaber R, Majerle A, Jerala R, Benčina M., Sensors (Basel) 13(12), 2013
PMID: 24287545
130 References
Daten bereitgestellt von Europe PubMed Central.
Response of the in vivo chlorophyll fluorescence spectrum to environmental factors and laser excitation wavelength
Agati G.., 1998
Agati G.., 1998
Directed evolution of a monomeric, bright and photostable version of Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging.
Ai HW, Henderson JN, Remington SJ, Campbell RE., Biochem. J. 400(3), 2006
PMID: 16859491
Ai HW, Henderson JN, Remington SJ, Campbell RE., Biochem. J. 400(3), 2006
PMID: 16859491
Forster distances for fluorescence resonant energy transfer between mCherry and other visible fluorescent proteins.
Akrap N, Seidel T, Barisas BG., Anal. Biochem. 402(1), 2010
PMID: 20347671
Akrap N, Seidel T, Barisas BG., Anal. Biochem. 402(1), 2010
PMID: 20347671
The origin of lignin fluorescence
Albinsson B., Li S., Lundquist K., Stomberg R.., 1999
Albinsson B., Li S., Lundquist K., Stomberg R.., 1999
Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral.
Baird GS, Zacharias DA, Tsien RY., Proc. Natl. Acad. Sci. U.S.A. 97(22), 2000
PMID: 11050229
Baird GS, Zacharias DA, Tsien RY., Proc. Natl. Acad. Sci. U.S.A. 97(22), 2000
PMID: 11050229
Combination of novel green fluorescent protein mutant TSapphire and DsRed variant mOrange to set up a versatile in planta FRET-FLIM assay.
Bayle V, Nussaume L, Bhat RA., Plant Physiol. 148(1), 2008
PMID: 18621983
Bayle V, Nussaume L, Bhat RA., Plant Physiol. 148(1), 2008
PMID: 18621983
A phosphatidylinositol-3-kinase-dependent signal transition regulates ARF1 and ARF6 during Fcgamma receptor-mediated phagocytosis.
Beemiller P, Hoppe AD, Swanson JA., PLoS Biol. 4(6), 2006
PMID: 16669702
Beemiller P, Hoppe AD, Swanson JA., PLoS Biol. 4(6), 2006
PMID: 16669702
FRET and FLIM applications in plants
Bhat R.., 2009
Bhat R.., 2009
The visible touch: in planta visualization of protein-protein interactions by fluorophore-based methods.
Bhat RA, Lahaye T, Panstruga R., Plant Methods 2(), 2006
PMID: 16800872
Bhat RA, Lahaye T, Panstruga R., Plant Methods 2(), 2006
PMID: 16800872
Dark proteins disturb multichromophore coupling in tetrameric fluorescent proteins.
Blum C, Meixner AJ, Subramaniam V., J Biophotonics 4(1-2), 2010
PMID: 20635430
Blum C, Meixner AJ, Subramaniam V., J Biophotonics 4(1-2), 2010
PMID: 20635430
A monomeric red fluorescent protein.
Campbell RE, Tour O, Palmer AE, Steinbach PA, Baird GS, Zacharias DA, Tsien RY., Proc. Natl. Acad. Sci. U.S.A. 99(12), 2002
PMID: 12060735
Campbell RE, Tour O, Palmer AE, Steinbach PA, Baird GS, Zacharias DA, Tsien RY., Proc. Natl. Acad. Sci. U.S.A. 99(12), 2002
PMID: 12060735
Dynamic imaging of glucose flux impedance using FRET sensors in wild-type Arabidopsis plants.
Chaudhuri B, Hormann F, Frommer WB., J. Exp. Bot. 62(7), 2011
PMID: 21266495
Chaudhuri B, Hormann F, Frommer WB., J. Exp. Bot. 62(7), 2011
PMID: 21266495
Characterization of spectral FRET imaging microscopy for monitoring nuclear protein interactions.
Chen Y, Mauldin JP, Day RN, Periasamy A., J Microsc 228(Pt 2), 2007
PMID: 17970914
Chen Y, Mauldin JP, Day RN, Periasamy A., J Microsc 228(Pt 2), 2007
PMID: 17970914
Measurement of FRET efficiency and ratio of donor to acceptor concentration in living cells.
Chen H, Puhl HL 3rd, Koushik SV, Vogel SS, Ikeda SR., Biophys. J. 91(5), 2006
PMID: 16815904
Chen H, Puhl HL 3rd, Koushik SV, Vogel SS, Ikeda SR., Biophys. J. 91(5), 2006
PMID: 16815904
Resonance energy transfer
Cheung H.., 1991
Cheung H.., 1991
A novel far-red bimolecular fluorescence complementation system that allows for efficient visualization of protein interactions under physiological conditions.
Chu J, Zhang Z, Zheng Y, Yang J, Qin L, Lu J, Huang ZL, Zeng S, Luo Q., Biosens Bioelectron 25(1), 2009
PMID: 19596565
Chu J, Zhang Z, Zheng Y, Yang J, Qin L, Lu J, Huang ZL, Zeng S, Luo Q., Biosens Bioelectron 25(1), 2009
PMID: 19596565
Dual-channel photobleaching FRET microscopy for improved resolution of protein association states in living cells.
Clayton AH, Klonis N, Cody SH, Nice EC., Eur. Biophys. J. 34(1), 2004
PMID: 15232659
Clayton AH, Klonis N, Cody SH, Nice EC., Eur. Biophys. J. 34(1), 2004
PMID: 15232659
Förster resonance energy transfer- FRET what it is, why do it, and how it's done
Clegg R.., 2009
Clegg R.., 2009
Forster resonance energy transfer demonstrates a flavonoid metabolon in living plant cells that displays competitive interactions between enzymes.
Crosby KC, Pietraszewska-Bogiel A, Gadella TW Jr, Winkel BS., FEBS Lett. 585(14), 2011
PMID: 21669202
Crosby KC, Pietraszewska-Bogiel A, Gadella TW Jr, Winkel BS., FEBS Lett. 585(14), 2011
PMID: 21669202
Understanding, improving and using green fluorescent proteins.
Cubitt AB, Heim R, Adams SR, Boyd AE, Gross LA, Tsien RY., Trends Biochem. Sci. 20(11), 1995
PMID: 8578587
Cubitt AB, Heim R, Adams SR, Boyd AE, Gross LA, Tsien RY., Trends Biochem. Sci. 20(11), 1995
PMID: 8578587
In vivo HIV-1 Rev multimerization in the nucleolus and cytoplasm identified by fluorescence resonance energy transfer.
Daelemans D, Costes SV, Cho EH, Erwin-Cohen RA, Lockett S, Pavlakis GN., J. Biol. Chem. 279(48), 2004
PMID: 15294891
Daelemans D, Costes SV, Cho EH, Erwin-Cohen RA, Lockett S, Pavlakis GN., J. Biol. Chem. 279(48), 2004
PMID: 15294891
Fluorescence resonance energy transfer of GFP and YFP by spectral imaging and quantitative acceptor photobleaching.
Dinant C, van Royen ME, Vermeulen W, Houtsmuller AB., J Microsc 231(Pt 1), 2008
PMID: 18638193
Dinant C, van Royen ME, Vermeulen W, Houtsmuller AB., J Microsc 231(Pt 1), 2008
PMID: 18638193
Cell damage and reactive oxygen species production induced by fluorescence microscopy: effect on mitosis and guidelines for non-invasive fluorescence microscopy.
Dixit R, Cyr R., Plant J. 36(2), 2003
PMID: 14535891
Dixit R, Cyr R., Plant J. 36(2), 2003
PMID: 14535891
Deconvolution of fluorescence spectra: contribution to the structural analysis of complex molecules.
Djikanovic D, Kalauzi A, Jeremic M, Micic M, Radotic K., Colloids Surf B Biointerfaces 54(2), 2006
PMID: 17134884
Djikanovic D, Kalauzi A, Jeremic M, Micic M, Radotic K., Colloids Surf B Biointerfaces 54(2), 2006
PMID: 17134884
Imaging FRET standards by steady-state fluorescence and lifetime methods.
Domingo B, Sabariegos R, Picazo F, Llopis J., Microsc. Res. Tech. 70(12), 2007
PMID: 17722057
Domingo B, Sabariegos R, Picazo F, Llopis J., Microsc. Res. Tech. 70(12), 2007
PMID: 17722057
Fluorescence lifetime imaging microscopy (FLIM) to quantify protein-protein interactions inside cells.
Duncan RR., Biochem. Soc. Trans. 34(Pt 5), 2006
PMID: 17052173
Duncan RR., Biochem. Soc. Trans. 34(Pt 5), 2006
PMID: 17052173
Ultrastructure of acridine alkaloid idioblasts in roots and cell cultures of Ruta graveolens
Eilert U., Wolters B., Constabel F.., 1986
Eilert U., Wolters B., Constabel F.., 1986
DsRed as a potential FRET partner with CFP and GFP.
Erickson MG, Moon DL, Yue DT., Biophys. J. 85(1), 2003
PMID: 12829514
Erickson MG, Moon DL, Yue DT., Biophys. J. 85(1), 2003
PMID: 12829514
Split mCherry as a new red bimolecular fluorescence complementation system for visualizing protein-protein interactions in living cells.
Fan JY, Cui ZQ, Wei HP, Zhang ZP, Zhou YF, Wang YP, Zhang XE., Biochem. Biophys. Res. Commun. 367(1), 2007
PMID: 18158915
Fan JY, Cui ZQ, Wei HP, Zhang ZP, Zhou YF, Wang YP, Zhang XE., Biochem. Biophys. Res. Commun. 367(1), 2007
PMID: 18158915
Energiewanderung und Fluoreszenz
Förster T.., 1946
Förster T.., 1946
Intermolecular energy migration and fluorescence
Förster T.., 1948
Förster T.., 1948
The single T65S mutation generates brighter cyan fluorescent proteins with increased photostability and pH insensitivity.
Fredj A, Pasquier H, Demachy I, Jonasson G, Levy B, Derrien V, Bousmah Y, Manoussaris G, Wien F, Ridard J, Erard M, Merola F., PLoS ONE 7(11), 2012
PMID: 23133673
Fredj A, Pasquier H, Demachy I, Jonasson G, Levy B, Derrien V, Bousmah Y, Manoussaris G, Wien F, Ridard J, Erard M, Merola F., PLoS ONE 7(11), 2012
PMID: 23133673
GFP-based FRET microscopy in living plant cells.
Gadella TW Jr, van der Krogt GN , Bisseling T., Trends Plant Sci. 4(7), 1999
PMID: 10407445
Gadella TW Jr, van der Krogt GN , Bisseling T., Trends Plant Sci. 4(7), 1999
PMID: 10407445
Three-chromophore FRET microscopy to analyze multiprotein interactions in living cells.
Galperin E, Verkhusha VV, Sorkin A., Nat. Methods 1(3), 2004
PMID: 15782196
Galperin E, Verkhusha VV, Sorkin A., Nat. Methods 1(3), 2004
PMID: 15782196
Light resonance energy transfer-based methods in the study of G protein-coupled receptor oligomerization.
Gandia J, Lluis C, Ferre S, Franco R, Ciruela F., Bioessays 30(1), 2008
PMID: 18081019
Gandia J, Lluis C, Ferre S, Franco R, Ciruela F., Bioessays 30(1), 2008
PMID: 18081019
Betaxanthins as pigments responsible for visible fluorescence in flowers.
Gandia-Herrero F, Escribano J, Garcia-Carmona F., Planta 222(4), 2005
PMID: 16177911
Gandia-Herrero F, Escribano J, Garcia-Carmona F., Planta 222(4), 2005
PMID: 16177911
A dark yellow fluorescent protein (YFP)-based Resonance Energy-Accepting Chromoprotein (REACh) for Forster resonance energy transfer with GFP.
Ganesan S, Ameer-Beg SM, Ng TT, Vojnovic B, Wouters FS., Proc. Natl. Acad. Sci. U.S.A. 103(11), 2006
PMID: 16537489
Ganesan S, Ameer-Beg SM, Ng TT, Vojnovic B, Wouters FS., Proc. Natl. Acad. Sci. U.S.A. 103(11), 2006
PMID: 16537489
Time domain FLIM: theory, instrumentation, and data analysis
Gerritsen H., Agronskaia A., Bader A., Esposito A.., 2009
Gerritsen H., Agronskaia A., Bader A., Esposito A.., 2009
Carotenoid fluorescence
Gillbro T., Cogdell R.., 1989
Gillbro T., Cogdell R.., 1989
Bright cyan fluorescent protein variants identified by fluorescence lifetime screening.
Goedhart J, van Weeren L, Hink MA, Vischer NO, Jalink K, Gadella TW Jr., Nat. Methods 7(2), 2010
PMID: 20081836
Goedhart J, van Weeren L, Hink MA, Vischer NO, Jalink K, Gadella TW Jr., Nat. Methods 7(2), 2010
PMID: 20081836
Sensitive detection of p65 homodimers using red-shifted and fluorescent protein-based FRET couples.
Goedhart J, Vermeer JE, Adjobo-Hermans MJ, van Weeren L, Gadella TW Jr., PLoS ONE 2(10), 2007
PMID: 17925859
Goedhart J, Vermeer JE, Adjobo-Hermans MJ, van Weeren L, Gadella TW Jr., PLoS ONE 2(10), 2007
PMID: 17925859
Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%.
Goedhart J, von Stetten D, Noirclerc-Savoye M, Lelimousin M, Joosen L, Hink MA, van Weeren L, Gadella TW Jr, Royant A., Nat Commun 3(), 2012
PMID: 22434194
Goedhart J, von Stetten D, Noirclerc-Savoye M, Lelimousin M, Joosen L, Hink MA, van Weeren L, Gadella TW Jr, Royant A., Nat Commun 3(), 2012
PMID: 22434194
Quantitative fluorescence resonance energy transfer measurements using fluorescence microscopy.
Gordon GW, Berry G, Liang XH, Levine B, Herman B., Biophys. J. 74(5), 1998
PMID: 9591694
Gordon GW, Berry G, Liang XH, Levine B, Herman B., Biophys. J. 74(5), 1998
PMID: 9591694
Reducing the environmental sensitivity of yellow fluorescent protein. Mechanism and applications.
Griesbeck O, Baird GS, Campbell RE, Zacharias DA, Tsien RY., J. Biol. Chem. 276(31), 2001
PMID: 11387331
Griesbeck O, Baird GS, Campbell RE, Zacharias DA, Tsien RY., J. Biol. Chem. 276(31), 2001
PMID: 11387331
SWISS-MODEL and the Swiss-PdbViewer: an environment for comparative protein modeling.
Guex N, Peitsch MC., Electrophoresis 18(15), 1997
PMID: 9504803
Guex N, Peitsch MC., Electrophoresis 18(15), 1997
PMID: 9504803
Removal of a cryptic intron and subcellular localization of green fluorescent protein are required to mark transgenic Arabidopsis plants brightly.
Haseloff J, Siemering KR, Prasher DC, Hodge S., Proc. Natl. Acad. Sci. U.S.A. 94(6), 1997
PMID: 9122158
Haseloff J, Siemering KR, Prasher DC, Hodge S., Proc. Natl. Acad. Sci. U.S.A. 94(6), 1997
PMID: 9122158
Triple FRET: a tool for studying long-range molecular interactions.
Haustein E, Jahnz M, Schwille P., Chemphyschem 4(7), 2003
PMID: 12901306
Haustein E, Jahnz M, Schwille P., Chemphyschem 4(7), 2003
PMID: 12901306
TRAF3 forms heterotrimers with TRAF2 and modulates its ability to mediate NF-{kappa}B activation.
He L, Grammer AC, Wu X, Lipsky PE., J. Biol. Chem. 279(53), 2004
PMID: 15383523
He L, Grammer AC, Wu X, Lipsky PE., J. Biol. Chem. 279(53), 2004
PMID: 15383523
Determination of tumor necrosis factor receptor-associated factor trimerization in living cells by CFP->YFP->mRFP FRET detected by flow cytometry.
He L, Wu X, Simone J, Hewgill D, Lipsky PE., Nucleic Acids Res. 33(6), 2005
PMID: 15805120
He L, Wu X, Simone J, Hewgill D, Lipsky PE., Nucleic Acids Res. 33(6), 2005
PMID: 15805120
Molecular spectroscopy and dynamics of intrinsically fluorescent proteins: coral red (dsRed) and yellow (Citrine).
Heikal AA, Hess ST, Baird GS, Tsien RY, Webb WW., Proc. Natl. Acad. Sci. U.S.A. 97(22), 2000
PMID: 11050231
Heikal AA, Hess ST, Baird GS, Tsien RY, Webb WW., Proc. Natl. Acad. Sci. U.S.A. 97(22), 2000
PMID: 11050231
Signal transfer in the plant plasma membrane: phospholipase A(2) is regulated via an inhibitory Gα protein and a cyclophilin.
Heinze M, Herre M, Massalski C, Hermann I, Conrad U, Roos W., Biochem. J. 450(3), 2013
PMID: 23252374
Heinze M, Herre M, Massalski C, Hermann I, Conrad U, Roos W., Biochem. J. 450(3), 2013
PMID: 23252374
Imaging protein-protein interactions in living cells.
Hink MA, Bisselin T, Visser AJ., Plant Mol. Biol. 50(6), 2002
PMID: 12516859
Hink MA, Bisselin T, Visser AJ., Plant Mol. Biol. 50(6), 2002
PMID: 12516859
Fluorescence resonance energy transfer-based stoichiometry in living cells.
Hoppe A, Christensen K, Swanson JA., Biophys. J. 83(6), 2002
PMID: 12496132
Hoppe A, Christensen K, Swanson JA., Biophys. J. 83(6), 2002
PMID: 12496132
Enhancement of folates in plants through metabolic engineering.
Hossain T, Rosenberg I, Selhub J, Kishore G, Beachy R, Schubert K., Proc. Natl. Acad. Sci. U.S.A. 101(14), 2004
PMID: 15044686
Hossain T, Rosenberg I, Selhub J, Kishore G, Beachy R, Schubert K., Proc. Natl. Acad. Sci. U.S.A. 101(14), 2004
PMID: 15044686
An improved mRFP1 adds red to bimolecular fluorescence complementation.
Jach G, Pesch M, Richter K, Frings S, Uhrig JF., Nat. Methods 3(8), 2006
PMID: 16862132
Jach G, Pesch M, Richter K, Frings S, Uhrig JF., Nat. Methods 3(8), 2006
PMID: 16862132
Filter FRET: quantitative imaging of sensitized emission
Jalink K., van J.., 2009
Jalink K., van J.., 2009
Fluorescence resonance energy transfer from cyan to yellow fluorescent protein detected by acceptor photobleaching using confocal microscopy and a single laser.
Karpova TS, Baumann CT, He L, Wu X, Grammer A, Lipsky P, Hager GL, McNally JG., J Microsc 209(Pt 1), 2003
PMID: 12535185
Karpova TS, Baumann CT, He L, Wu X, Grammer A, Lipsky P, Hager GL, McNally JG., J Microsc 209(Pt 1), 2003
PMID: 12535185
The membrane-tethered transcription factor ANAC089 serves as redox-dependent suppressor of stromal ascorbate peroxidase gene expression.
Klein P, Seidel T, Stocker B, Dietz KJ., Front Plant Sci 3(), 2012
PMID: 23162559
Klein P, Seidel T, Stocker B, Dietz KJ., Front Plant Sci 3(), 2012
PMID: 23162559
Carotenoid fluorescence in Dunaliella salina.
Kleinegris DM, van Es MA, Janssen M, Brandenburg WA, Wijffels RH., J. Appl. Phycol. 22(5), 2010
PMID: 20835349
Kleinegris DM, van Es MA, Janssen M, Brandenburg WA, Wijffels RH., J. Appl. Phycol. 22(5), 2010
PMID: 20835349
Subcellular distribution of the V-ATPase complex in plant cells, and in vivo localisation of the 100 kDa subunit VHA-a within the complex.
Kluge C, Seidel T, Bolte S, Sharma SS, Hanitzsch M, Satiat-Jeunemaitre B, Ross J, Sauer M, Golldack D, Dietz KJ., BMC Cell Biol. 5(), 2004
PMID: 15310389
Kluge C, Seidel T, Bolte S, Sharma SS, Hanitzsch M, Satiat-Jeunemaitre B, Ross J, Sauer M, Golldack D, Dietz KJ., BMC Cell Biol. 5(), 2004
PMID: 15310389
Anomalous surplus energy transfer observed with multiple FRET acceptors.
Koushik SV, Blank PS, Vogel SS., PLoS ONE 4(11), 2009
PMID: 19946626
Koushik SV, Blank PS, Vogel SS., PLoS ONE 4(11), 2009
PMID: 19946626
Cerulean, Venus, and VenusY67C FRET reference standards.
Koushik SV, Chen H, Thaler C, Puhl HL 3rd, Vogel SS., Biophys. J. 91(12), 2006
PMID: 17040988
Koushik SV, Chen H, Thaler C, Puhl HL 3rd, Vogel SS., Biophys. J. 91(12), 2006
PMID: 17040988
FRET-based genetically encoded sensors allow high-resolution live cell imaging of Ca²⁺ dynamics.
Krebs M, Held K, Binder A, Hashimoto K, Den Herder G, Parniske M, Kudla J, Schumacher K., Plant J. 69(1), 2011
PMID: 21910770
Krebs M, Held K, Binder A, Hashimoto K, Den Herder G, Parniske M, Kudla J, Schumacher K., Plant J. 69(1), 2011
PMID: 21910770
Visible fluorescent proteins for FRET
Kremers G., Goedhart J.., 2009
Kremers G., Goedhart J.., 2009
Cyan and yellow super fluorescent proteins with improved brightness, protein folding, and FRET Forster radius.
Kremers GJ, Goedhart J, van Munster EB, Gadella TW Jr., Biochemistry 45(21), 2006
PMID: 16716067
Kremers GJ, Goedhart J, van Munster EB, Gadella TW Jr., Biochemistry 45(21), 2006
PMID: 16716067
Combined bimolecular fluorescence complementation and Forster resonance energy transfer reveals ternary SNARE complex formation in living plant cells.
Kwaaitaal M, Keinath NF, Pajonk S, Biskup C, Panstruga R., Plant Physiol. 152(3), 2010
PMID: 20071602
Kwaaitaal M, Keinath NF, Pajonk S, Biskup C, Panstruga R., Plant Physiol. 152(3), 2010
PMID: 20071602
Lakowicz J.., 2006
Improving FRET dynamic range with bright green and red fluorescent proteins.
Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin MZ., Nat. Methods 9(10), 2012
PMID: 22961245
Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin MZ., Nat. Methods 9(10), 2012
PMID: 22961245
Imaging molecular interactions in cells by dynamic and static fluorescence anisotropy (rFLIM and emFRET).
Lidke DS, Nagy P, Barisas BG, Heintzmann R, Post JN, Lidke KA, Clayton AH, Arndt-Jovin DJ, Jovin TM., Biochem. Soc. Trans. 31(Pt 5), 2003
PMID: 14505472
Lidke DS, Nagy P, Barisas BG, Heintzmann R, Post JN, Lidke KA, Clayton AH, Arndt-Jovin DJ, Jovin TM., Biochem. Soc. Trans. 31(Pt 5), 2003
PMID: 14505472
Some secrets of fluorescent proteins: distinct bleaching in various mounting fluids and photoactivation of cyan fluorescent proteins at YFP-excitation.
Malkani N, Schmid JA., PLoS ONE 6(4), 2011
PMID: 21490932
Malkani N, Schmid JA., PLoS ONE 6(4), 2011
PMID: 21490932
An improved cerulean fluorescent protein with enhanced brightness and reduced reversible photoswitching.
Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, Day RN, Wachter RM, Davidson MW, Rizzo MA., PLoS ONE 6(3), 2011
PMID: 21479270
Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, Day RN, Wachter RM, Davidson MW, Rizzo MA., PLoS ONE 6(3), 2011
PMID: 21479270
Novel lambda FRET spectral confocal microscopy imaging method.
Megias D, Marrero R, Martinez Del Peso B, Garcia MA, Bravo-Cordero JJ, Garcia-Grande A, Santos A, Montoya MC., Microsc. Res. Tech. 72(1), 2009
PMID: 18785251
Megias D, Marrero R, Martinez Del Peso B, Garcia MA, Bravo-Cordero JJ, Garcia-Grande A, Santos A, Montoya MC., Microsc. Res. Tech. 72(1), 2009
PMID: 18785251
Dynamic and quantitative Ca2+ measurements using improved cameleons.
Miyawaki A, Griesbeck O, Heim R, Tsien RY., Proc. Natl. Acad. Sci. U.S.A. 96(5), 1999
PMID: 10051607
Miyawaki A, Griesbeck O, Heim R, Tsien RY., Proc. Natl. Acad. Sci. U.S.A. 96(5), 1999
PMID: 10051607
Monitoring protein conformations and interactions by fluorescence resonance energy transfer between mutants of green fluorescent protein.
Miyawaki A, Tsien RY., Meth. Enzymol. 327(), 2000
PMID: 11045004
Miyawaki A, Tsien RY., Meth. Enzymol. 327(), 2000
PMID: 11045004
Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin.
Miyawaki A, Llopis J, Heim R, McCaffery JM, Adams JA, Ikura M, Tsien RY., Nature 388(6645), 1997
PMID: 9278050
Miyawaki A, Llopis J, Heim R, McCaffery JM, Adams JA, Ikura M, Tsien RY., Nature 388(6645), 1997
PMID: 9278050
Red fluorescent protein from Discosoma as a fusion tag and a partner for fluorescence resonance energy transfer.
Mizuno H, Sawano A, Eli P, Hama H, Miyawaki A., Biochemistry 40(8), 2001
PMID: 11327872
Mizuno H, Sawano A, Eli P, Hama H, Miyawaki A., Biochemistry 40(8), 2001
PMID: 11327872
Highly sensitive and quantitative FRET-FLIM imaging in single dendritic spines using improved non-radiative YFP.
Murakoshi H, Lee SJ, Yasuda R., Brain Cell Biol 36(1-4), 2008
PMID: 18512154
Murakoshi H, Lee SJ, Yasuda R., Brain Cell Biol 36(1-4), 2008
PMID: 18512154
Multiple redox and non-redox interactions define 2-Cys peroxiredoxin as a regulatory hub in the chloroplast.
Muthuramalingam M, Seidel T, Laxa M, Nunes de Miranda SM, Gartner F, Stroher E, Kandlbinder A, Dietz KJ., Mol Plant 2(6), 2009
PMID: 19995730
Muthuramalingam M, Seidel T, Laxa M, Nunes de Miranda SM, Gartner F, Stroher E, Kandlbinder A, Dietz KJ., Mol Plant 2(6), 2009
PMID: 19995730
A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications.
Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, Miyawaki A., Nat. Biotechnol. 20(1), 2002
PMID: 11753368
Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, Miyawaki A., Nat. Biotechnol. 20(1), 2002
PMID: 11753368
Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins.
Nagai T, Yamada S, Tominaga T, Ichikawa M, Miyawaki A., Proc. Natl. Acad. Sci. U.S.A. 101(29), 2004
PMID: 15247428
Nagai T, Yamada S, Tominaga T, Ichikawa M, Miyawaki A., Proc. Natl. Acad. Sci. U.S.A. 101(29), 2004
PMID: 15247428
Quantitative imaging with fluorescent biosensors.
Okumoto S, Jones A, Frommer WB., Annu Rev Plant Biol 63(), 2012
PMID: 22404462
Okumoto S, Jones A, Frommer WB., Annu Rev Plant Biol 63(), 2012
PMID: 22404462
Characterization of vacuolar transport of the endogenous alkaloid berberine in Coptis japonica.
Otani M, Shitan N, Sakai K, Martinoia E, Sato F, Yazaki K., Plant Physiol. 138(4), 2005
PMID: 16024684
Otani M, Shitan N, Sakai K, Martinoia E, Sato F, Yazaki K., Plant Physiol. 138(4), 2005
PMID: 16024684
Quantitative FRET analysis by fast acquisition time domain FLIM at high spatial resolution in living cells.
Padilla-Parra S, Auduge N, Coppey-Moisan M, Tramier M., Biophys. J. 95(6), 2008
PMID: 18539634
Padilla-Parra S, Auduge N, Coppey-Moisan M, Tramier M., Biophys. J. 95(6), 2008
PMID: 18539634
Quantitative comparison of different fluorescent protein couples for fast FRET-FLIM acquisition.
Padilla-Parra S, Auduge N, Lalucque H, Mevel JC, Coppey-Moisan M, Tramier M., Biophys. J. 97(8), 2009
PMID: 19843469
Padilla-Parra S, Auduge N, Lalucque H, Mevel JC, Coppey-Moisan M, Tramier M., Biophys. J. 97(8), 2009
PMID: 19843469
Forster distances between green fluorescent protein pairs.
Patterson GH, Piston DW, Barisas BG., Anal. Biochem. 284(2), 2000
PMID: 10964438
Patterson GH, Piston DW, Barisas BG., Anal. Biochem. 284(2), 2000
PMID: 10964438
Multiphoton-FLIM quantification of the EGFP-mRFP1 FRET pair for localization of membrane receptor-kinase interactions.
Peter M, Ameer-Beg SM, Hughes MK, Keppler MD, Prag S, Marsh M, Vojnovic B, Ng T., Biophys. J. 88(2), 2004
PMID: 15531633
Peter M, Ameer-Beg SM, Hughes MK, Keppler MD, Prag S, Marsh M, Vojnovic B, Ng T., Biophys. J. 88(2), 2004
PMID: 15531633
FRET-based in vivo screening for protein folding and increased protein stability.
Philipps B, Hennecke J, Glockshuber R., J. Mol. Biol. 327(1), 2003
PMID: 12614622
Philipps B, Hennecke J, Glockshuber R., J. Mol. Biol. 327(1), 2003
PMID: 12614622
Fluorescent protein FRET: the good, the bad and the ugly.
Piston DW, Kremers GJ., Trends Biochem. Sci. 32(9), 2007
PMID: 17764955
Piston DW, Kremers GJ., Trends Biochem. Sci. 32(9), 2007
PMID: 17764955
Autofluorescence of atmospheric bioaerosols – fluorescent biomolecules and potential interferences
Pöhlker C., Huffman J., Pöschl U.., 2011
Pöhlker C., Huffman J., Pöschl U.., 2011
Flow cytometric and fluorometric methods of quantifying and characterizing apoptotic cell death
Poot M., Pierce R., Kavanagh T.., 2002
Poot M., Pierce R., Kavanagh T.., 2002
Enhanced yellow fluorescent protein photoconversion to a cyan fluorescent protein-like species is sensitive to thermal and diffusion conditions.
Raarup MK, Fjorback AW, Jensen SM, Muller HK, Kjaergaard MM, Poulsen H, Wiborg O, Nyengaard JR., J Biomed Opt 14(3), 2009
PMID: 19566331
Raarup MK, Fjorback AW, Jensen SM, Muller HK, Kjaergaard MM, Poulsen H, Wiborg O, Nyengaard JR., J Biomed Opt 14(3), 2009
PMID: 19566331
Protein interaction quantified in vivo by spectrally resolved fluorescence resonance energy transfer.
Raicu V, Jansma DB, Miller RJ, Friesen JD., Biochem. J. 385(Pt 1), 2005
PMID: 15352875
Raicu V, Jansma DB, Miller RJ, Friesen JD., Biochem. J. 385(Pt 1), 2005
PMID: 15352875
Optimization of pairings and detection conditions for measurement of FRET between cyan and yellow fluorescent proteins.
Rizzo MA, Springer G, Segawa K, Zipfel WR, Piston DW., Microsc. Microanal. 12(3), 2006
PMID: 17481360
Rizzo MA, Springer G, Segawa K, Zipfel WR, Piston DW., Microsc. Microanal. 12(3), 2006
PMID: 17481360
An improved cyan fluorescent protein variant useful for FRET.
Rizzo MA, Springer GH, Granada B, Piston DW., Nat. Biotechnol. 22(4), 2004
PMID: 14990965
Rizzo MA, Springer GH, Granada B, Piston DW., Nat. Biotechnol. 22(4), 2004
PMID: 14990965
Vital autofluorescence: application to the study of plant living cells
Roshchina V.., 2012
Roshchina V.., 2012
Changes in pollen autofluorescence induced by ozone
Roshchina V., Karnaukhov V.., 1999
Roshchina V., Karnaukhov V.., 1999
Oligomerization of the human serotonin transporter and of the rat GABA transporter 1 visualized by fluorescence resonance energy transfer microscopy in living cells.
Schmid JA, Scholze P, Kudlacek O, Freissmuth M, Singer EA, Sitte HH., J. Biol. Chem. 276(6), 2000
PMID: 11071889
Schmid JA, Scholze P, Kudlacek O, Freissmuth M, Singer EA, Sitte HH., J. Biol. Chem. 276(6), 2000
PMID: 11071889
The cellular energization state affects peripheral stalk stability of plant vacuolar H+-ATPase and impairs vacuolar acidification.
Schnitzer D, Seidel T, Sander T, Golldack D, Dietz KJ., Plant Cell Physiol. 52(5), 2011
PMID: 21474463
Schnitzer D, Seidel T, Sander T, Golldack D, Dietz KJ., Plant Cell Physiol. 52(5), 2011
PMID: 21474463
Fluorescent proteins for single-molecule fluorescence applications.
Seefeldt B, Kasper R, Seidel T, Tinnefeld P, Dietz KJ, Heilemann M, Sauer M., J Biophotonics 1(1), 2008
PMID: 19343637
Seefeldt B, Kasper R, Seidel T, Tinnefeld P, Dietz KJ, Heilemann M, Sauer M., J Biophotonics 1(1), 2008
PMID: 19343637
Mapping of C-termini of V-ATPase subunits by in vivo-FRET measurements.
Seidel T, Golldack D, Dietz KJ., FEBS Lett. 579(20), 2005
PMID: 16061227
Seidel T, Golldack D, Dietz KJ., FEBS Lett. 579(20), 2005
PMID: 16061227
Colocalization and FRET-analysis of subunits c and a of the vacuolar H+-ATPase in living plant cells.
Seidel T, Kluge C, Hanitzsch M, Ross J, Sauer M, Dietz KJ, Golldack D., J. Biotechnol. 112(1-2), 2004
PMID: 15288951
Seidel T, Kluge C, Hanitzsch M, Ross J, Sauer M, Dietz KJ, Golldack D., J. Biotechnol. 112(1-2), 2004
PMID: 15288951
In vivo analysis of the 2-Cys peroxiredoxin oligomeric state by two-step FRET.
Seidel T, Seefeldt B, Sauer M, Dietz KJ., J. Biotechnol. 149(4), 2010
PMID: 20615439
Seidel T, Seefeldt B, Sauer M, Dietz KJ., J. Biotechnol. 149(4), 2010
PMID: 20615439
Halide and proton binding kinetics of yellow fluorescent protein variants.
Seward HE, Basran J, Denton R, Pfuhl M, Muskett FW, Bagshaw CR., Biochemistry 52(14), 2013
PMID: 23514090
Seward HE, Basran J, Denton R, Pfuhl M, Muskett FW, Bagshaw CR., Biochemistry 52(14), 2013
PMID: 23514090
Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein.
Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY., Nat. Biotechnol. 22(12), 2004
PMID: 15558047
Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY., Nat. Biotechnol. 22(12), 2004
PMID: 15558047
Additional correction for energy transfer efficiency calculation in filter-based Forster resonance energy transfer microscopy for more accurate results.
Sun Y, Periasamy A., J Biomed Opt 15(2), 2010
PMID: 20459222
Sun Y, Periasamy A., J Biomed Opt 15(2), 2010
PMID: 20459222
Three-color spectral FRET microscopy localizes three interacting proteins in living cells.
Sun Y, Wallrabe H, Booker CF, Day RN, Periasamy A., Biophys. J. 99(4), 2010
PMID: 20713013
Sun Y, Wallrabe H, Booker CF, Day RN, Periasamy A., Biophys. J. 99(4), 2010
PMID: 20713013
Computer program for analyzing donor photobleaching FRET image series.
Szentesi G, Vereb G, Horvath G, Bodnar A, Fabian A, Matko J, Gaspar R, Damjanovich S, Matyus L, Jenei A., Cytometry A 67(2), 2005
PMID: 16163694
Szentesi G, Vereb G, Horvath G, Bodnar A, Fabian A, Matko J, Gaspar R, Damjanovich S, Matyus L, Jenei A., Cytometry A 67(2), 2005
PMID: 16163694
An ultramarine fluorescent protein with increased photostability and pH insensitivity.
Tomosugi W, Matsuda T, Tani T, Nemoto T, Kotera I, Saito K, Horikawa K, Nagai T., Nat. Methods 6(5), 2009
PMID: 19349978
Tomosugi W, Matsuda T, Tani T, Nemoto T, Kotera I, Saito K, Horikawa K, Nagai T., Nat. Methods 6(5), 2009
PMID: 19349978
Sensitivity of CFP/YFP and GFP/mCherry pairs to donor photobleaching on FRET determination by fluorescence lifetime imaging microscopy in living cells.
Tramier M, Zahid M, Mevel JC, Masse MJ, Coppey-Moisan M., Microsc. Res. Tech. 69(11), 2006
PMID: 16941642
Tramier M, Zahid M, Mevel JC, Masse MJ, Coppey-Moisan M., Microsc. Res. Tech. 69(11), 2006
PMID: 16941642
Photoconversion of YFP into a CFP-like species during acceptor photobleaching FRET experiments.
Valentin G, Verheggen C, Piolot T, Neel H, Coppey-Moisan M, Bertrand E., Nat. Methods 2(11), 2005
PMID: 16278647
Valentin G, Verheggen C, Piolot T, Neel H, Coppey-Moisan M, Bertrand E., Nat. Methods 2(11), 2005
PMID: 16278647
Fluorescence resonance energy transfer (FRET) measurement by gradual acceptor photobleaching.
Van Munster EB, Kremers GJ, Adjobo-Hermans MJ, Gadella TW Jr., J Microsc 218(Pt 3), 2005
PMID: 15958019
Van Munster EB, Kremers GJ, Adjobo-Hermans MJ, Gadella TW Jr., J Microsc 218(Pt 3), 2005
PMID: 15958019
Correcting confocal acquisition to optimize imaging of fluorescence resonance energy transfer by sensitized emission.
van Rheenen J, Langeslag M, Jalink K., Biophys. J. 86(4), 2004
PMID: 15041688
van Rheenen J, Langeslag M, Jalink K., Biophys. J. 86(4), 2004
PMID: 15041688
The Arabidopsis B-type response regulator 18 homomerizes and positively regulates cytokinin responses.
Veerabagu M, Elgass K, Kirchler T, Huppenberger P, Harter K, Chaban C, Mira-Rodado V., Plant J. 72(5), 2012
PMID: 22775331
Veerabagu M, Elgass K, Kirchler T, Huppenberger P, Harter K, Chaban C, Mira-Rodado V., Plant J. 72(5), 2012
PMID: 22775331
Probing plasma membrane microdomains in cowpea protoplasts using lipidated GFP-fusion proteins and multimode FRET microscopy.
Vermeer JE, Van Munster EB, Vischer NO, Gadella TW Jr., J Microsc 214(Pt 2), 2004
PMID: 15102066
Vermeer JE, Van Munster EB, Vischer NO, Gadella TW Jr., J Microsc 214(Pt 2), 2004
PMID: 15102066
Frequency domain FLIM theory, instrumentation, and data analysis
Verveer P., Hanley Q.., 2009
Verveer P., Hanley Q.., 2009
Immunofluorescence microscopy for localization of Arabidopsis chloroplast proteins.
Vitha S, Osteryoung KW., Methods Mol. Biol. 774(), 2011
PMID: 21822831
Vitha S, Osteryoung KW., Methods Mol. Biol. 774(), 2011
PMID: 21822831
The impact of heterogeneity and dark acceptor states on FRET: implications for using fluorescent protein donors and acceptors.
Vogel SS, Nguyen TA, van der Meer BW, Blank PS., PLoS ONE 7(11), 2012
PMID: 23152925
Vogel SS, Nguyen TA, van der Meer BW, Blank PS., PLoS ONE 7(11), 2012
PMID: 23152925
Imaging protein molecules using FRET and FLIM microscopy.
Wallrabe H, Periasamy A., Curr. Opin. Biotechnol. 16(1), 2005
PMID: 15722011
Wallrabe H, Periasamy A., Curr. Opin. Biotechnol. 16(1), 2005
PMID: 15722011
Alanine zipper-like coiled-coil domains are necessary for homotypic dimerization of plant GAGA-factors in the nucleus and nucleolus.
Wanke D, Hohenstatt ML, Dynowski M, Bloss U, Hecker A, Elgass K, Hummel S, Hahn A, Caesar K, Schleifenbaum F, Harter K, Berendzen KW., PLoS ONE 6(2), 2011
PMID: 21347358
Wanke D, Hohenstatt ML, Dynowski M, Bloss U, Hecker A, Elgass K, Hummel S, Hahn A, Caesar K, Schleifenbaum F, Harter K, Berendzen KW., PLoS ONE 6(2), 2011
PMID: 21347358
Two-step FRET as a structural tool.
Watrob HM, Pan CP, Barkley MD., J. Am. Chem. Soc. 125(24), 2003
PMID: 12797808
Watrob HM, Pan CP, Barkley MD., J. Am. Chem. Soc. 125(24), 2003
PMID: 12797808
Mitochondrial cysteine synthase complex regulates O-acetylserine biosynthesis in plants.
Wirtz M, Beard KF, Lee CP, Boltz A, Schwarzlander M, Fuchs C, Meyer AJ, Heeg C, Sweetlove LJ, Ratcliffe RG, Hell R., J. Biol. Chem. 287(33), 2012
PMID: 22730323
Wirtz M, Beard KF, Lee CP, Boltz A, Schwarzlander M, Fuchs C, Meyer AJ, Heeg C, Sweetlove LJ, Ratcliffe RG, Hell R., J. Biol. Chem. 287(33), 2012
PMID: 22730323
Kaede for detection of protein oligomerization.
Wolf H, Barisas BG, Dietz KJ, Seidel T., Mol Plant 6(5), 2013
PMID: 23430050
Wolf H, Barisas BG, Dietz KJ, Seidel T., Mol Plant 6(5), 2013
PMID: 23430050
Elements of transcriptional machinery are compatible among plants and mammals.
Wolf A, Akrap N, Marg B, Galliardt H, Heiligentag M, Humpert F, Sauer M, Kaltschmidt B, Kaltschmidt C, Seidel T., PLoS ONE 8(1), 2013
PMID: 23326494
Wolf A, Akrap N, Marg B, Galliardt H, Heiligentag M, Humpert F, Sauer M, Kaltschmidt B, Kaltschmidt C, Seidel T., PLoS ONE 8(1), 2013
PMID: 23326494
The fluorescence of organic natural products
Wolfbeis O.., 1985
Wolfbeis O.., 1985
Fluorescence fluctuation spectroscopy of mCherry in living cells.
Wu B, Chen Y, Muller JD., Biophys. J. 96(6), 2009
PMID: 19289064
Wu B, Chen Y, Muller JD., Biophys. J. 96(6), 2009
PMID: 19289064
Reliable and global measurement of fluorescence resonance energy transfer using fluorescence microscopes.
Xia Z, Liu Y., Biophys. J. 81(4), 2001
PMID: 11566809
Xia Z, Liu Y., Biophys. J. 81(4), 2001
PMID: 11566809
The molecular structure of green fluorescent protein.
Yang F, Moss LG, Phillips GN Jr., Nat. Biotechnol. 14(10), 1996
PMID: 9631087
Yang F, Moss LG, Phillips GN Jr., Nat. Biotechnol. 14(10), 1996
PMID: 9631087
Photobleaching-corrected FRET efficiency imaging of live cells.
Zal T, Gascoigne NR., Biophys. J. 86(6), 2004
PMID: 15189889
Zal T, Gascoigne NR., Biophys. J. 86(6), 2004
PMID: 15189889
Efficiently folding and circularly permuted variants of the Sapphire mutant of GFP.
Zapata-Hommer O, Griesbeck O., BMC Biotechnol. 3(), 2003
PMID: 12769828
Zapata-Hommer O, Griesbeck O., BMC Biotechnol. 3(), 2003
PMID: 12769828
Export
Markieren/ Markierung löschen
Markierte Publikationen
Web of Science
Dieser Datensatz im Web of Science®Quellen
PMID: 24194740
PubMed | Europe PMC
Suchen in