Polymyxin P is the active principle in suppressing phytopathogenic Erwinia spp. by the biocontrol rhizobacterium Paenibacillus polymyxa M-1

Niu B, Vater J, Rückert C, Blom J, Lehmann M, Ru J-J, Chen X-H, Wang Q, Borriss R (2013)
BMC microbiology 13(1): 137.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
DOI
Autor*in
Niu, Ben; Vater, Joachim; Rückert, ChristianUniBi ; Blom, JochenUniBi; Lehmann, Maik; Ru, Jin-Jiang; Chen, Xiao-Hua; Wang, Qi; Borriss, Rainer
Einrichtung
Technische Fakultät > Computational Genomics
Centrum für Biotechnologie > Arbeitsgruppe A. Goesmann
Centrum für Biotechnologie > Technologieplattformen > Bioinformatics Resource Facility
Abstract / Bemerkung
BACKGROUND: Nine gene clusters dedicated to nonribosomal synthesis of secondary metabolites with possible antimicrobial action, including polymyxin and fusaricidin, were detected within the whole genome sequence of the plant growth-promoting rhizobacterium (PGPR) Paenibacillus polymyxa M-1. To survey the antimicrobial compounds expressed by M-1 we analyzed the active principle suppressing phytopathogenic Erwinia spp. RESULTS: P. polymyxa M-1 suppressed the growth of phytopathogenic Erwinia amylovora Ea 273, and E. carotovora, the causative agents of fire blight and soft rot, respectively. By MALDI-TOF mass spectrometry and reversed-phase high-performance liquid chromatography (RP-HPLC), two antibacterial compounds bearing molecular masses of 1190.9 Da and 1176.9 Da were detected as being the two components of polymyxin P, polymyxin P1 and P2, respectively. The active principle acting against the two Erwinia strains was isolated from TLC plates and identified by postsource decay (PSD)-MALDI-TOF mass spectrometry as polymyxin P1 and polymyxin P2. These findings were corroborated by domain structure analysis of the polymyxin (pmx) gene cluster detected in the M-1 chromosome which revealed that corresponding to the chemical structure of polymyxin P, the gene cluster is encoding D-Phe in position 6 and L-Thr in position 7. CONCLUSIONS: Identical morphological changes in the cell wall of the bacterial phytopathogens treated with either crude polymyxin P or culture supernatant of M-1 corroborated that polymyxin P is the main component of the biocontrol effect exerted by strain M-1 against phytopathogenic Erwinia spp.
Erscheinungsjahr
2013
Zeitschriftentitel
BMC microbiology
Band
13
Ausgabe
1
Art.-Nr.
137
ISSN
1471-2180
Page URI
https://pub.uni-bielefeld.de/record/2607974

Zitieren

Niu B, Vater J, Rückert C, et al. Polymyxin P is the active principle in suppressing phytopathogenic Erwinia spp. by the biocontrol rhizobacterium Paenibacillus polymyxa M-1. BMC microbiology. 2013;13(1): 137.
Niu, B., Vater, J., Rückert, C., Blom, J., Lehmann, M., Ru, J. - J., Chen, X. - H., et al. (2013). Polymyxin P is the active principle in suppressing phytopathogenic Erwinia spp. by the biocontrol rhizobacterium Paenibacillus polymyxa M-1. BMC microbiology, 13(1), 137. doi:10.1186/1471-2180-13-137
Niu, Ben, Vater, Joachim, Rückert, Christian, Blom, Jochen, Lehmann, Maik, Ru, Jin-Jiang, Chen, Xiao-Hua, Wang, Qi, and Borriss, Rainer. 2013. “Polymyxin P is the active principle in suppressing phytopathogenic Erwinia spp. by the biocontrol rhizobacterium Paenibacillus polymyxa M-1”. BMC microbiology 13 (1): 137.
Niu, B., Vater, J., Rückert, C., Blom, J., Lehmann, M., Ru, J. - J., Chen, X. - H., Wang, Q., and Borriss, R. (2013). Polymyxin P is the active principle in suppressing phytopathogenic Erwinia spp. by the biocontrol rhizobacterium Paenibacillus polymyxa M-1. BMC microbiology 13:137.
Niu, B., et al., 2013. Polymyxin P is the active principle in suppressing phytopathogenic Erwinia spp. by the biocontrol rhizobacterium Paenibacillus polymyxa M-1. BMC microbiology, 13(1): 137.
B. Niu, et al., “Polymyxin P is the active principle in suppressing phytopathogenic Erwinia spp. by the biocontrol rhizobacterium Paenibacillus polymyxa M-1”, BMC microbiology, vol. 13, 2013, : 137.
Niu, B., Vater, J., Rückert, C., Blom, J., Lehmann, M., Ru, J.-J., Chen, X.-H., Wang, Q., Borriss, R.: Polymyxin P is the active principle in suppressing phytopathogenic Erwinia spp. by the biocontrol rhizobacterium Paenibacillus polymyxa M-1. BMC microbiology. 13, : 137 (2013).
Niu, Ben, Vater, Joachim, Rückert, Christian, Blom, Jochen, Lehmann, Maik, Ru, Jin-Jiang, Chen, Xiao-Hua, Wang, Qi, and Borriss, Rainer. “Polymyxin P is the active principle in suppressing phytopathogenic Erwinia spp. by the biocontrol rhizobacterium Paenibacillus polymyxa M-1”. BMC microbiology 13.1 (2013): 137.

20 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Chronicle of a Soil Bacterium: Paenibacillus polymyxa E681 as a Tiny Guardian of Plant and Human Health.
Jeong H, Choi SK, Ryu CM, Park SH., Front Microbiol 10(), 2019
PMID: 30930873
Fengycin Produced by Bacillus amyloliquefaciens FZB42 Inhibits Fusarium graminearum Growth and Mycotoxins Biosynthesis.
Hanif A, Zhang F, Li P, Li C, Xu Y, Zubair M, Zhang M, Jia D, Zhao X, Liang J, Majid T, Yan J, Farzand A, Wu H, Gu Q, Gao X., Toxins (Basel) 11(5), 2019
PMID: 31137632
Genome Mining of the Lipopeptide Biosynthesis of Paenibacillus polymyxa E681 in Combination with Mass Spectrometry: Discovery of the Lipoheptapeptide Paenilipoheptin.
Vater J, Herfort S, Doellinger J, Weydmann M, Borriss R, Lasch P., Chembiochem 19(7), 2018
PMID: 29369464
Purification, Characterization and in vitro Evaluation of Polymyxin A From Paenibacillus dendritiformis: An Underexplored Member of the Polymyxin Family.
Jangra M, Randhawa HK, Kaur M, Srivastava A, Maurya N, Patil PP, Jaswal P, Arora A, Patil PB, Raje M, Nandanwar H., Front Microbiol 9(), 2018
PMID: 30532748
Fusaricidins from Paenibacillus polymyxa M-1, a family of lipohexapeptides of unusual complexity-a mass spectrometric study.
Vater J, Herfort S, Doellinger J, Weydmann M, Dietel K, Faetke S, Lasch P., J Mass Spectrom 52(1), 2017
PMID: 27714901
Dynamics of bacterial class Bacilli in the deepest valley lake of Kashmir-the Manasbal Lake.
Shafi S, Kamili AN, Shah MA, Bandh SA, Dar R., Microb Pathog 104(), 2017
PMID: 28087491
Seeds of the Wild Progenitor of Maize Possess Bacteria That Antagonize Foodborne Pathogens.
Shehata HR, Griffiths MW, Raizada MN., Foodborne Pathog Dis 14(4), 2017
PMID: 28186422
Simplified and representative bacterial community of maize roots.
Niu B, Paulson JN, Zheng X, Kolter R., Proc Natl Acad Sci U S A 114(12), 2017
PMID: 28275097
Fengycin produced by Bacillus subtilis 9407 plays a major role in the biocontrol of apple ring rot disease.
Fan H, Ru J, Zhang Y, Wang Q, Li Y., Microbiol Res 199(), 2017
PMID: 28454713
Recent advances and perspectives in the design and development of polymyxins.
Rabanal F, Cajal Y., Nat Prod Rep 34(7), 2017
PMID: 28628170
The antibacterial activity and modes of LI-F type antimicrobial peptides against Bacillus cereus in vitro.
Han J, Zhao S, Ma Z, Gao L, Liu H, Muhammad U, Lu Z, Lv F, Bie X., J Appl Microbiol 123(3), 2017
PMID: 28650559
Synthetic Cyclolipopeptides Selective against Microbial, Plant and Animal Cell Targets by Incorporation of D-Amino Acids or Histidine.
Vilà S, Badosa E, Montesinos E, Planas M, Feliu L., PLoS One 11(3), 2016
PMID: 27008420
Draft Genome Sequence of Bacillus cereus 905, a Plant Growth-Promoting Rhizobacterium of Wheat.
Ding H, Niu B, Fan H, Li Y, Wang Q., Genome Announc 4(3), 2016
PMID: 27257205
Polymyxin: Alternative Mechanisms of Action and Resistance.
Trimble MJ, Mlynárčik P, Kolář M, Hancock RE., Cold Spring Harb Perspect Med 6(10), 2016
PMID: 27503996
Genome mining: Prediction of lipopeptides and polyketides from Bacillus and related Firmicutes.
Aleti G, Sessitsch A, Brader G., Comput Struct Biotechnol J 13(), 2015
PMID: 25893081
Full genome sequence of Brevibacillus laterosporus strain B9, a biological control strain isolated from Zhejiang, China.
Li G, Xu J, Wu L, Ren D, Ye W, Dong G, Zhu L, Zeng D, Guo L., J Biotechnol 207(), 2015
PMID: 26022423
Characterization of Novel Fusaricidins Produced by Paenibacillus polymyxa-M1 Using MALDI-TOF Mass Spectrometry.
Vater J, Niu B, Dietel K, Borriss R., J Am Soc Mass Spectrom 26(9), 2015
PMID: 26100395
Complete Genome Sequence of Paenibacillus polymyxa CR1, a Plant Growth-Promoting Bacterium Isolated from the Corn Rhizosphere Exhibiting Potential for Biocontrol, Biomass Degradation, and Biofuel Production.
Eastman AW, Weselowski B, Nathoo N, Yuan ZC., Genome Announc 2(1), 2014
PMID: 24459277
Complete Genome Sequence of Paenibacillus polymyxa SQR-21, a Plant Growth-Promoting Rhizobacterium with Antifungal Activity and Rhizosphere Colonization Ability.
Li S, Yang D, Qiu M, Shao J, Guo R, Shen B, Yin X, Zhang R, Zhang N, Shen Q., Genome Announc 2(2), 2014
PMID: 24723719
Comparative and genetic analysis of the four sequenced Paenibacillus polymyxa genomes reveals a diverse metabolism and conservation of genes relevant to plant-growth promotion and competitiveness.
Eastman AW, Heinrichs DE, Yuan ZC., BMC Genomics 15(), 2014
PMID: 25280501

51 References

Daten bereitgestellt von Europe PubMed Central.

Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test
AUTHOR UNKNOWN, 1993
Response of crested wheatgrass (Agropyron cristatum L.), perennial ryegrass (Lolium perenne) and white clover (Trifolium repens L.) to inoculation with Bacillus polymyxa.
Holl FB, Chanway CP, Turkington R, Radley RA., Soil Biol. Biochem. 20(1), 1988
PMID: ADL87095745
Genome sequence of the polymyxin-producing plant-probiotic rhizobacterium Paenibacillus polymyxa E681.
Kim JF, Jeong H, Park SY, Kim SB, Park YK, Choi SK, Ryu CM, Hur CG, Ghim SY, Oh TK, Kim JJ, Park CS, Park SH., J. Bacteriol. 192(22), 2010
PMID: 20851896
A plant growth promoting rhizobacterium, Paenibacillus polymyxa strain GBR-1, suppresses root-knot nematode.
Khan Z, Kim SG, Jeon YH, Khan HU, Son SH, Kim YH., Bioresour. Technol. 99(8), 2007
PMID: 17706411
Plant growth-promoting rhizobacteria, Paenibacillus polymyxa and Paenibacillus lentimorbus suppress disease complex caused by root-knot nematode and fusarium wilt fungus.
Son SH, Khan Z, Kim SG, Kim YH., J. Appl. Microbiol. 107(2), 2009
PMID: 19457027
Antibiosis plays a role in the context of direct interaction during antagonism of Paenibacillus polymyxa towards Fusarium oxysporum.
Dijksterhuis J, Sanders M, Gorris LG, Smid EJ., J. Appl. Microbiol. 86(1), 1999
PMID: 10030009
Concurrent selection for microbial suppression of Fusarium graminearum, Fusarium head blight and deoxynivalenol in wheat.
He J, Boland GJ, Zhou T., J. Appl. Microbiol. 106(6), 2009
PMID: 19298518
Colonization of peanut roots by biofilm-forming Paenibacillus polymyxa initiates biocontrol against crown rot disease.
Haggag WM, Timmusk S., J. Appl. Microbiol. 104(4), 2007
PMID: 18005030
Isolation of an antifungal Paenibacillus strain HT16 from locusts and purification of its medium-dependent antagonistic component.
Zhou WW, Huang JX, Niu TG., J. Appl. Microbiol. 105(3), 2008
PMID: 18422553
Paenibacillus polymyxa produces fusaricidin-type antifungal antibiotics active against Leptosphaeria maculans, the causative agent of blackleg disease of canola.
Beatty PH, Jensen SE., Can. J. Microbiol. 48(2), 2002
PMID: 11958569
Paenibacillus polymyxa antagonizes oomycete plant pathogens Phytophthora palmivora and Pythium aphanidermatum.
Timmusk S, van West P, Gow NA, Huffstutler RP., J. Appl. Microbiol. 106(5), 2009
PMID: 19226403
Isolation and partial characterization of antibacterial lipopeptide produced by Paenibacillus polymyxa HKA-15 against phytopathogen Xanthomonas campestris pv. phaseoli M-5
Mageshwaran V, Walia S, Annapurna K., World J. Microbiol. Biotechnol. 28(3), 2012
PMID: IND44746205
Biological control and plant growth promoting capacity of rhizobacteria on pepper under greenhouse and field conditions.
Hahm MS, Sumayo M, Hwang YJ, Jeon SA, Park SJ, Lee JY, Ahn JH, Kim BS, Ryu CM, Ghim SY., J. Microbiol. 50(3), 2012
PMID: 22752900
Polymyxin P, new antibiotics of polymyxin group.
Kimura Y, Murai E, Fujisawa M, Tatsuki T, Nobue F., J. Antibiot. 22(9), 1969
PMID: 4391193
Isolation, structural characterization, and properties of mattacin (polymyxin M), a cyclic peptide antibiotic produced by Paenibacillus kobensis M.
Martin NI, Hu H, Moake MM, Churey JJ, Whittal R, Worobo RW, Vederas JC., J. Biol. Chem. 278(15), 2003
PMID: 12569104
Isolation and structural characterization of colistin components.
Orwa JA, Govaerts C, Busson R, Roets E, Van Schepdael A, Hoogmartens J., J. Antibiot. 54(7), 2001
PMID: 11560379
Polymyxin E, a new peptide antibiotic.
Parker WL, Rathnum ML, Dean LD, Nimeck MW, Brown WE, Meyers E., J. Antibiot. 30(9), 1977
PMID: 200598
Isolation of two new polymyxin group antibiotics. (Studies on antibiotics from the genus Bacillus. XX).
Shoji J, Hinoo H, Wakisaka Y, Koizumi K, Mayama M, Matsuura S., J. Antibiot. 30(12), 1977
PMID: 202582
The structure of polymyxin S. (Studies on antibiotics from the genus Bacillus. XXI).
Shoji J, Kato T, Hinoo H., J. Antibiot. 30(12), 1977
PMID: 202583
The structure of polymyxin T1. (Studies on antibiotics from the genus Bacillus. XXII).
Shoji J, Kato T, Hinoo H., J. Antibiot. 30(12), 1977
PMID: 202584
Polymyxin b: controlled biosynthesis.
Withander L, Heding H., J. Antibiot. 29(7), 1976
PMID: 182664
Isolation and identification of a Paenibacillus polymyxa strain that coproduces a novel lantibiotic and polymyxin.
He Z, Kisla D, Zhang L, Yuan C, Green-Church KB, Yousef AE., Appl. Environ. Microbiol. 73(1), 2006
PMID: 17071789
Gavaserin and saltavalin, new peptide antibiotics produced by Bacillus polymyxa.
Pichard B, Larue JP, Thouvenot D., FEMS Microbiol. Lett. 133(3), 1995
PMID: 8522137
Jolipeptin, a new peptide antibiotic. II. The mode of action of jolipeptin.
Ito M, Koyama Y., J. Antibiot. 25(5), 1972
PMID: 4625359
A new antibiotic, gatavalin. I. Isolation and characterization.
Nakajima N, Chihara S, Koyama Y., J. Antibiot. 25(4), 1972
PMID: 4559273
Fusaricidin A, a new depsipeptide antibiotic produced by Bacillus polymyxa KT-8. Taxonomy, fermentation, isolation, structure elucidation and biological activity.
Kajimura Y, Kaneda M., J. Antibiot. 49(2), 1996
PMID: 8621351
Isolation and characterisation of fusaricidin-type compound-producing strain of Paenibacillus polymyxa SQR-21 active against Fusarium oxysporum f.sp. nevium
Raza W, Yang X, Wu H, Wang Y, Xu Y, Shen Q., Eur. J. Plant Pathol. 125(3), 2009
PMID: IND44268566
Identification of a polymyxin synthetase gene cluster of Paenibacillus polymyxa and heterologous expression of the gene in Bacillus subtilis.
Choi SK, Park SY, Kim R, Kim SB, Lee CH, Kim JF, Park SH., J. Bacteriol. 191(10), 2009
PMID: 19304848
Effectiveness of polymyxin B-immobilized fiber column in sepsis: a systematic review.
Cruz DN, Perazella MA, Bellomo R, de Cal M, Polanco N, Corradi V, Lentini P, Nalesso F, Ueno T, Ranieri VM, Ronco C., Crit Care 11(2), 2007
PMID: 17448226
Structure--activity relationships of polymyxin antibiotics.
Velkov T, Thompson PE, Nation RL, Li J., J. Med. Chem. 53(5), 2010
PMID: 19874036
Biosynthesis of nonribosomal peptides1.
Finking R, Marahiel MA., Annu. Rev. Microbiol. 58(), 2004
PMID: 15487945
Paenibacillus polymyxa PKB1 produces variants of polymyxin B-type antibiotics.
Shaheen M, Li J, Ross AC, Vederas JC, Jensen SE., Chem. Biol. 18(12), 2011
PMID: 22195566
Isolation and identification of endophytic bacteria antagonistic to wheat sharp eyespot disease
AUTHOR UNKNOWN, 2008
The genome of the plant growth-promoting rhizobacterium Paenibacillus polymyxa M-1 contains nine sites dedicated to nonribosomal synthesis of lipopeptides and polyketides.
Niu B, Rueckert C, Blom J, Wang Q, Borriss R., J. Bacteriol. 193(20), 2011
PMID: 21952539
Difficidin and bacilysin produced by plant-associated Bacillus amyloliquefaciens are efficient in controlling fire blight disease.
Chen XH, Scholz R, Borriss M, Junge H, Mogel G, Kunz S, Borriss R., J. Biotechnol. 140(1-2), 2008
PMID: 19061923
Complete genome sequence of Paenibacillus polymyxa SC2, a strain of plant growth-promoting Rhizobacterium with broad-spectrum antimicrobial activity.
Ma M, Wang C, Ding Y, Li L, Shen D, Jiang X, Guan D, Cao F, Chen H, Feng R, Wang X, Ge Y, Yao L, Bing X, Yang X, Li J, Du B., J. Bacteriol. 193(1), 2010
PMID: 21037012
Matrix-assisted laser desorption ionization--time of flight mass spectrometry of lipopeptide biosurfactants in whole cells and culture filtrates of Bacillus subtilis C-1 isolated from petroleum sludge.
Vater J, Kablitz B, Wilde C, Franke P, Mehta N, Cameotra SS., Appl. Environ. Microbiol. 68(12), 2002
PMID: 12450846
Identification and functional analysis of the fusaricidin biosynthetic gene of Paenibacillus polymyxa E681.
Choi SK, Park SY, Kim R, Lee CH, Kim JF, Park SH., Biochem. Biophys. Res. Commun. 365(1), 2007
PMID: 17980146
Structural and functional characterization of three polyketide synthase gene clusters in Bacillus amyloliquefaciens FZB 42.
Chen XH, Vater J, Piel J, Franke P, Scholz R, Schneider K, Koumoutsi A, Hitzeroth G, Grammel N, Strittmatter AW, Gottschalk G, Sussmuth RD, Borriss R., J. Bacteriol. 188(11), 2006
PMID: 16707694
Action of polymyxin B on bacterial membranes: morphological changes in the cytoplasm and in the outer membrane of Salmonella typhimurium and Escherichia coli B.
Schindler PR, Teuber M., Antimicrob. Agents Chemother. 8(1), 1975
PMID: 169730
Electron microscope observations on the effects of polymixin B sulfate on cell walls of Chlamydia psittaci.
Matsumoto A, Higashi N, Tamura A., J. Bacteriol. 113(1), 1973
PMID: 4120068
Electron microscopic studies on mode of action of polymyxin.
Koike M, Iida K, Matsuo T., J. Bacteriol. 97(1), 1969
PMID: 4303542
NRPSpredictor2--a web server for predicting NRPS adenylation domain specificity.
Rottig M, Medema MH, Blin K, Weber T, Rausch C, Kohlbacher O., Nucleic Acids Res. 39(Web Server issue), 2011
PMID: 21558170
Phylogenetic analysis of condensation domains in NRPS sheds light on their functional evolution.
Rausch C, Hoof I, Weber T, Wohlleben W, Huson DH., BMC Evol. Biol. 7(), 2007
PMID: 17506888
Determination of cell mass and polymyxin using multi-wavelength fluorescence.
Eliasson Lantz A, Jorgensen P, Poulsen E, Lindemann C, Olsson L., J. Biotechnol. 121(4), 2005
PMID: 16157411

AUTHOR UNKNOWN, 1935
Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA.
Marchesi JR, Sato T, Weightman AJ, Martin TA, Fry JC, Hiom SJ, Dymock D, Wade WG., Appl. Environ. Microbiol. 64(2), 1998
PMID: 9464425
Nutritional Requirements of Bacillus alvei and Bacillus para-alvei.
Katznelson H, Lochhead AG., J. Bacteriol. 53(1), 1947
PMID: 16561254
Bacillomycin; an antibiotic from Bacillus subtilis active against pathogenic fungi.
LANDY M, WARREN GH., Proc. Soc. Exp. Biol. Med. 67(4), 1948
PMID: 18860010
"Whole cell"--matrix-assisted laser desorption ionization-time of flight-mass spectrometry, an emerging technique for efficient screening of biocombinatorial libraries of natural compounds-present state of research.
Vater J, Gao X, Hitzeroth G, Wilde C, Franke P., Comb. Chem. High Throughput Screen. 6(6), 2003
PMID: 14529380
Effect of polymyxin on the ultrastructure of the outer membrane of wild-type and polymyxin-resistant strain of Salmonella.
Lounatmaa K, Makela PH, Sarvas M., J. Bacteriol. 127(3), 1976
PMID: 182675
Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®
Quellen

PMID: 23773687
PubMed | Europe PMC

Suchen in

Google Scholar