Corynebacterium glutamicum promoters: a practical approach

Pátek M, Holátko J, Busche T, Kalinowski J, Nešvera J (2013)
Microbial Biotechnology 6(2): 103-117.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Pátek, Miroslav; Holátko, Jiří; Busche, TobiasUniBi; Kalinowski, JörnUniBi; Nešvera, Jan
Abstract / Bemerkung
Transcription initiation is the key step in gene expression in bacteria, and it is therefore studied for both theoretical and practical reasons. Promoters, the traffic lights of transcription initiation, are used as construction elements in biotechnological efforts to coordinate 'green waves' in the metabolic pathways leading to the desired metabolites. Detailed analyses of Corynebacterium glutamicum promoters have already provided large amounts of data on their structures, regulatory mechanisms and practical capabilities in metabolic engineering. In this minireview the main aspects of promoter studies, the methods developed for their analysis and their practical use in C. glutamicum are discussed. These include definitions of the consensus sequences of the distinct promoter classes, promoter localization and characterization, activity measurements, the functions of transcriptional regulators and examples of practical uses of constitutive, inducible and modified promoters in biotechnology. The implications of the introduction of novel techniques, such as in vitro transcription and RNA sequencing, to C. glutamicum promoter studies are outlined.
Microbial Biotechnology
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Pátek M, Holátko J, Busche T, Kalinowski J, Nešvera J. Corynebacterium glutamicum promoters: a practical approach. Microbial Biotechnology. 2013;6(2):103-117.
Pátek, M., Holátko, J., Busche, T., Kalinowski, J., & Nešvera, J. (2013). Corynebacterium glutamicum promoters: a practical approach. Microbial Biotechnology, 6(2), 103-117. doi:10.1111/1751-7915.12019
Pátek, Miroslav, Holátko, Jiří, Busche, Tobias, Kalinowski, Jörn, and Nešvera, Jan. 2013. “Corynebacterium glutamicum promoters: a practical approach”. Microbial Biotechnology 6 (2): 103-117.
Pátek, M., Holátko, J., Busche, T., Kalinowski, J., and Nešvera, J. (2013). Corynebacterium glutamicum promoters: a practical approach. Microbial Biotechnology 6, 103-117.
Pátek, M., et al., 2013. Corynebacterium glutamicum promoters: a practical approach. Microbial Biotechnology, 6(2), p 103-117.
M. Pátek, et al., “Corynebacterium glutamicum promoters: a practical approach”, Microbial Biotechnology, vol. 6, 2013, pp. 103-117.
Pátek, M., Holátko, J., Busche, T., Kalinowski, J., Nešvera, J.: Corynebacterium glutamicum promoters: a practical approach. Microbial Biotechnology. 6, 103-117 (2013).
Pátek, Miroslav, Holátko, Jiří, Busche, Tobias, Kalinowski, Jörn, and Nešvera, Jan. “Corynebacterium glutamicum promoters: a practical approach”. Microbial Biotechnology 6.2 (2013): 103-117.

21 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Promoter library-based module combination (PLMC) technology for optimization of threonine biosynthesis in Corynebacterium glutamicum.
Wei L, Xu N, Wang Y, Zhou W, Han G, Ma Y, Liu J., Appl Microbiol Biotechnol 102(9), 2018
PMID: 29564525
Assignment of sigma factors of RNA polymerase to promoters in Corynebacterium glutamicum.
Dostálová H, Holátko J, Busche T, Rucká L, Rapoport A, Halada P, Nešvera J, Kalinowski J, Pátek M., AMB Express 7(1), 2017
PMID: 28651382
Expression of recombinant protein using Corynebacterium Glutamicum: progress, challenges and applications.
Liu X, Yang Y, Zhang W, Sun Y, Peng F, Jeffrey L, Harvey L, McNeil B, Bai Z., Crit Rev Biotechnol 36(4), 2016
PMID: 25714007
Regulons of global transcription factors in Corynebacterium glutamicum.
Toyoda K, Inui M., Appl Microbiol Biotechnol 100(1), 2016
PMID: 26496920
The contest for precursors: channelling L-isoleucine synthesis in Corynebacterium glutamicum without byproduct formation.
Vogt M, Krumbach K, Bang WG, van Ooyen J, Noack S, Klein B, Bott M, Eggeling L., Appl Microbiol Biotechnol 99(2), 2015
PMID: 25301583
Ribosome binding site libraries and pathway modules for shikimic acid synthesis with Corynebacterium glutamicum.
Zhang B, Zhou N, Liu YM, Liu C, Lou CB, Jiang CY, Liu SJ., Microb Cell Fact 14(), 2015
PMID: 25981633
Synthetic promoter libraries for Corynebacterium glutamicum.
Rytter JV, Helmark S, Chen J, Lezyk MJ, Solem C, Jensen PR., Appl Microbiol Biotechnol 98(6), 2014
PMID: 24458563
σ(ECF) factors of gram-positive bacteria: a focus on Bacillus subtilis and the CMNR group.
Souza BM, Castro TL, Carvalho RD, Seyffert N, Silva A, Miyoshi A, Azevedo V., Virulence 5(5), 2014
PMID: 24921931
Construction of a host-independent T7 expression system with small RNA regulation.
Wang G, Li Q, Xu D, Cui M, Sun X, Xu Y, Wang W., J Biotechnol 189(), 2014
PMID: 25193711

83 References

Daten bereitgestellt von Europe PubMed Central.

Altered metabolic flux due to deletion of odhA causes L-glutamate overproduction in Corynebacterium glutamicum.
Asakura Y, Kimura E, Usuda Y, Kawahara Y, Matsui K, Osumi T, Nakamatsu T., Appl. Environ. Microbiol. 73(4), 2006
PMID: 17158630
Metabolic flux engineering of L-lysine production in Corynebacterium glutamicum--over expression and modification of G6P dehydrogenase.
Becker J, Klopprogge C, Herold A, Zelder O, Bolten CJ, Wittmann C., J. Biotechnol. 132(2), 2007
PMID: 17624457
From zero to hero--design-based systems metabolic engineering of Corynebacterium glutamicum for L-lysine production.
Becker J, Zelder O, Hafner S, Schroder H, Wittmann C., Metab. Eng. 13(2), 2011
PMID: 21241816
Positively regulated expression of the Escherichia coli araBAD promoter in Corynebacterium glutamicum.
Ben-Samoun K, Leblon G, Reyes O., FEMS Microbiol. Lett. 174(1), 1999
PMID: 10234830
A high-throughput approach to identify genomic variants of bacterial metabolite producers at the single-cell level.
Binder S, Schendzielorz G, Stabler N, Krumbach K, Hoffmann K, Bott M, Eggeling L., Genome Biol. 13(5), 2012
PMID: 22640862
Molecular analysis of the Corynebacterium glutamicum gdh gene encoding glutamate dehydrogenase.
Bormann ER, Eikmanns BJ, Sahm H., Mol. Microbiol. 6(3), 1992
PMID: 1552846
The regulation of bacterial transcription initiation.
Browning DF, Busby SJ., Nat. Rev. Microbiol. 2(1), 2004
PMID: 15035009
Group 2 sigma factor SigB of Corynebacterium glutamicum positively regulates glucose metabolism under conditions of oxygen deprivation.
Ehira S, Shirai T, Teramoto H, Inui M, Yukawa H., Appl. Environ. Microbiol. 74(16), 2008
PMID: 18567683
Identification of succinate exporter in Corynebacterium glutamicum and its physiological roles under anaerobic conditions.
Fukui K, Koseki C, Yamamoto Y, Nakamura J, Sasahara A, Yuji R, Hashiguchi K, Usuda Y, Matsui K, Kojima H, Abe K., J. Biotechnol. 154(1), 2011
PMID: 21420450
Acetate metabolism and its regulation in Corynebacterium glutamicum.
Gerstmeir R, Wendisch VF, Schnicke S, Ruan H, Farwick M, Reinscheid D, Eikmanns BJ., J. Biotechnol. 104(1-3), 2003
PMID: 12948633
Multiple sigma subunits and the partitioning of bacterial transcription space.
Gruber TM, Gross CA., Annu. Rev. Microbiol. 57(), 2003
PMID: 14527287
A game with many players: control of gdh transcription in Corynebacterium glutamicum.
Hanssler E, Muller T, Palumbo K, Patek M, Brocker M, Kramer R, Burkovski A., J. Biotechnol. 142(2), 2009
PMID: 19394370
Metabolic engineering of the L-valine biosynthesis pathway in Corynebacterium glutamicum using promoter activity modulation.
Holatko J, Elisakova V, Prouza M, Sobotka M, Nesvera J, Patek M., J. Biotechnol. 139(3), 2008
PMID: 19121344
Construction of in vitro transcription system for Corynebacterium glutamicum and its use in the recognition of promoters of different classes.
Holatko J, Silar R, Rabatinova A, Sanderova H, Halada P, Nesvera J, Krasny L, Patek M., Appl. Microbiol. Biotechnol. 96(2), 2012
PMID: 22885668
Rational design of a Corynebacterium glutamicum pantothenate production strain and its characterization by metabolic flux analysis and genome-wide transcriptional profiling.
Huser AT, Chassagnole C, Lindley ND, Merkamm M, Guyonvarch A, Elisakova V, Patek M, Kalinowski J, Brune I, Puhler A, Tauch A., Appl. Environ. Microbiol. 71(6), 2005
PMID: 15933028
Transcriptional profiling of Corynebacterium glutamicum metabolism during organic acid production under oxygen deprivation conditions.
Inui M, Suda M, Okino S, Nonaka H, Puskas LG, Vertes AA, Yukawa H., Microbiology (Reading, Engl.) 153(Pt 8), 2007
PMID: 17660414
A recombinant Mycobacterium tuberculosis in vitro transcription system.
Jacques JF, Rodrigue S, Brzezinski R, Gaudreau L., FEMS Microbiol. Lett. 255(1), 2006
PMID: 16436073
Detection of prokaryotic promoters from the genomic distribution of hexanucleotide pairs.
Jacques PE, Rodrigue S, Gaudreau L, Goulet J, Brzezinski R., BMC Bioinformatics 7(), 2006
PMID: 17014715
Plasmid vectors for testing in vivo promoter activities in Corynebacterium glutamicum and Rhodococcus erythropolis.
Knoppova M, Phensaijai M, Vesely M, Zemanova M, Nesvera J, Patek M., Curr. Microbiol. 55(3), 2007
PMID: 17657537
A tetracycline inducible expression vector for Corynebacterium glutamicum allowing tightly regulable gene expression.
Lausberg F, Chattopadhyay AR, Heyer A, Eggeling L, Freudl R., Plasmid 68(2), 2012
PMID: 22587824
Characterization and use of catabolite-repressed promoters from gluconate genes in Corynebacterium glutamicum.
Letek M, Valbuena N, Ramos A, Ordonez E, Gil JA, Mateos LM., J. Bacteriol. 188(2), 2006
PMID: 16385030
The development and application of a single-cell biosensor for the detection of l-methionine and branched-chain amino acids.
Mustafi N, Grunberger A, Kohlheyer D, Bott M, Frunzke J., Metab. Eng. 14(4), 2012
PMID: 22583745
Plasmids and promoters in corynebacteria and their applications
Nešvera J, Pátek M, Burkovski A., 2008
Tools for genetic manipulations in Corynebacterium glutamicum and their applications.
Nesvera J, Patek M., Appl. Microbiol. Biotechnol. 90(5), 2011
PMID: 21519933
Production of L-lysine on different silage juices using genetically engineered Corynebacterium glutamicum
Neuner A, Wagner I, Sieker T, Ulber R, Schneider K, Peifer S, Heinzle E., 2012
Glutamine synthetases of Corynebacterium glutamicum: transcriptional control and regulation of activity.
Nolden L, Farwick M, Kramer R, Burkovski A., FEMS Microbiol. Lett. 201(1), 2001
PMID: 11445173
Isolation, evaluation and use of two strong, carbon source-inducible promoters from Corynebacterium glutamicum.
Okibe N, Suzuki N, Inui M, Yukawa H., Lett. Appl. Microbiol. 50(2), 2009
PMID: 20002569
Improved L-lysine production with Corynebacterium glutamicum and systemic insight into citrate synthase flux and activity.
van Ooyen J, Noack S, Bott M, Reth A, Eggeling L., Biotechnol. Bioeng. 109(8), 2012
PMID: 22392073
Sigma factors and promoters in Corynebacterium glutamicum.
Patek M, Nesvera J., J. Biotechnol. 154(2-3), 2011
PMID: 21277915
Promoters and plasmid vectors of Corynebacterium glutamicum
Pátek M, Nešvera J, Yukawa H, Inui M., 2012
Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif.
Patek M, Eikmanns BJ, Patek J, Sahm H., Microbiology (Reading, Engl.) 142 ( Pt 5)(), 1996
PMID: 8704969
Promoters of Corynebacterium glutamicum.
Patek M, Nesvera J, Guyonvarch A, Reyes O, Leblon G., J. Biotechnol. 104(1-3), 2003
PMID: 12948648
CoryneRegNet 6.0--Updated database content, new analysis methods and novel features focusing on community demands.
Pauling J, Rottger R, Tauch A, Azevedo V, Baumbach J., Nucleic Acids Res. 40(Database issue), 2011
PMID: 22080556
Biotechnological manufacture of lysine.
Pfefferle W, Mockel B, Bathe B, Marx A., Adv. Biochem. Eng. Biotechnol. 79(), 2003
PMID: 12523389
Molecular characterization of PrpR, the transcriptional activator of propionate catabolism in Corynebacterium glutamicum.
Plassmeier J, Persicke M, Puhler A, Sterthoff C, Ruckert C, Kalinowski J., J. Biotechnol. 159(1-2), 2011
PMID: 21933687
A propionate-inducible expression system based on the Corynebacterium glutamicum prpD2 promoter and PrpR activator and its application for redirection of amino acid biosynthesis pathways
Plassmeier J, Busche T, Molck S, Presicke M, Pühler A, Rückert S, Kalinowski J., 2012
The sigma factors of Mycobacterium tuberculosis.
Rodrigue S, Provvedi R, Jacques PE, Gaudreau L, Manganelli R., FEMS Microbiol. Rev. 30(6), 2006
PMID: 17064287
Analysis of RNA polymerase-promoter complex formation.
Ross W, Gourse RL., Methods 47(1), 2008
PMID: 18952176
The Zur regulon of Corynebacterium glutamicum ATCC 13032.
Schroder J, Jochmann N, Rodionov DA, Tauch A., BMC Genomics 11(), 2010
PMID: 20055984
The primary transcriptome of the major human pathogen Helicobacter pylori.
Sharma CM, Hoffmann S, Darfeuille F, Reignier J, Findeiss S, Sittka A, Chabas S, Reiche K, Hackermuller J, Reinhardt R, Stadler PF, Vogel J., Nature 464(7286), 2010
PMID: 20164839
Engineered Corynebacterium glutamicum as an endotoxin-free platform strain for lactate-based polyester production.
Song Y, Matsumoto K, Yamada M, Gohda A, Brigham CJ, Sinskey AJ, Taguchi S., Appl. Microbiol. Biotechnol. 93(5), 2011
PMID: 22127753
Development of novel cell surface display in Corynebacterium glutamicum using porin.
Tateno T, Hatada K, Tanaka T, Fukuda H, Kondo A., Appl. Microbiol. Biotechnol. 84(4), 2009
PMID: 19430772
Genetic control systems of Escherichia coli can confer inducible expression of cloned genes in coryneform bacteria
Tsuchiya M, Morinaga Y., 1988
Glucosamine as carbon source for amino acid-producing Corynebacterium glutamicum
Uhde A, Youn JW, Maeda T, Clermont L, Matano C, Krämer R., 2012
Integrating and autonomously replicating vectors for analysis of promoters in Corynebacterium glutamicum
Vašicová P, Abrhámová Z, Nešvera J, Pátek M, Sahm H, Eikmanns B., 1998
Analysis of the Corynebacterium glutamicum dapA promoter.
Vasicova P, Patek M, Nesvera J, Sahm H, Eikmanns B., J. Bacteriol. 181(19), 1999
PMID: 10498736
Postgenomic approaches to using corynebacteria as biocatalysts.
Vertes AA, Inui M, Yukawa H., Annu. Rev. Microbiol. 66(), 2012
PMID: 22803796
Emerging Corynebacterium glutamicum systems biology.
Wendisch VF, Bott M, Kalinowski J, Oldiges M, Wiechert W., J. Biotechnol. 124(1), 2006
PMID: 16406159
Engineering Corynebacterium glutamicum for the production of pyruvate.
Wieschalka S, Blombach B, Eikmanns BJ., Appl. Microbiol. Biotechnol. 94(2), 2012
PMID: 22228312
Identification and characterization of the dicarboxylate uptake system DccT in Corynebacterium glutamicum.
Youn JW, Jolkver E, Kramer R, Marin K, Wendisch VF., J. Bacteriol. 190(19), 2008
PMID: 18658264
Characterization of the dicarboxylate transporter DctA in Corynebacterium glutamicum.
Youn JW, Jolkver E, Kramer R, Marin K, Wendisch VF., J. Bacteriol. 191(17), 2009
PMID: 19581365
Development and application of an arabinose-inducible expression system by facilitating inducer uptake in Corynebacterium glutamicum.
Zhang Y, Shang X, Lai S, Zhang G, Liang Y, Wen T., Appl. Environ. Microbiol. 78(16), 2012
PMID: 22685153

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