Transcriptional regulation of the operon encoding stress-responsive ECF sigma factor SigH and its anti-sigma factor RshA, and control of its regulatory network in Corynebacterium glutamicum
Busche T, Silar R, Picmanova M, Patek M, Kalinowski J (2012)
BMC Genomics 13(1): 445.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
Download
Autor*in
Busche, TobiasUniBi;
Silar, Radoslav;
Picmanova, Martina;
Patek, Miroslav;
Kalinowski, JörnUniBi
Abstract / Bemerkung
Background
The expression of genes in Corynebacterium glutamicum, a Gram-positive non-pathogenic bacterium used mainly for the industrial production of amino acids, is regulated by seven different sigma factors of RNA polymerase, including the stress-responsive ECF-sigma factor SigH. The sigH gene is located in a gene cluster together with the rshA gene, putatively encoding an anti-sigma factor. The aim of this study was to analyze the transcriptional regulation of the sigH and rshA gene cluster and the effects of RshA on the SigH regulon, in order to refine the model describing the role of SigH and RshA during stress response.
Results
Transcription analyses revealed that the sigH gene and rshA gene are cotranscribed from four sigH housekeeping promoters in C. glutamicum. In addition, a SigH-controlled rshA promoter was found to only drive the transcription of the rshA gene. To test the role of the putative anti-sigma factor gene rshA under normal growth conditions, a C. glutamicum rshA deletion strain was constructed and used for genome-wide transcription profiling with DNA microarrays. In total, 83 genes organized in 61 putative transcriptional units, including those previously detected using sigH mutant strains, exhibited increased transcript levels in the rshA deletion mutant compared to its parental strain. The genes encoding proteins related to disulphide stress response, heat stress proteins, components of the SOS-response to DNA damage and proteasome components were the most markedly upregulated gene groups. Altogether six SigH-dependent promoters upstream of the identified genes were determined by primer extension and a refined consensus promoter consisting of 45 original promoter sequences was constructed.
Conclusions
The rshA gene codes for an anti-sigma factor controlling the function of the stress-responsive sigma factor SigH in C. glutamicum. Transcription of rshA from a SigH-dependent promoter may serve to quickly shutdown the SigH-dependent stress response after the cells have overcome the stress condition. Here we propose a model of the regulation of oxidative and heat stress response including redox homeostasis by SigH, RshA and the thioredoxin system.
Stichworte
Anti-sigma factor;
Corynebacterium glutamicum;
ECF sigma factor;
Promoter;
Microarray analysis
Erscheinungsjahr
2012
Zeitschriftentitel
BMC Genomics
Band
13
Ausgabe
1
Art.-Nr.
445
ISSN
1471-2164
Finanzierungs-Informationen
Open-Access-Publikationskosten wurden durch die Deutsche Forschungsgemeinschaft und die Universität Bielefeld gefördert.
Page URI
https://pub.uni-bielefeld.de/record/2528277
Zitieren
Busche T, Silar R, Picmanova M, Patek M, Kalinowski J. Transcriptional regulation of the operon encoding stress-responsive ECF sigma factor SigH and its anti-sigma factor RshA, and control of its regulatory network in Corynebacterium glutamicum. BMC Genomics. 2012;13(1): 445.
Busche, T., Silar, R., Picmanova, M., Patek, M., & Kalinowski, J. (2012). Transcriptional regulation of the operon encoding stress-responsive ECF sigma factor SigH and its anti-sigma factor RshA, and control of its regulatory network in Corynebacterium glutamicum. BMC Genomics, 13(1), 445. doi:10.1186/1471-2164-13-445
Busche, Tobias, Silar, Radoslav, Picmanova, Martina, Patek, Miroslav, and Kalinowski, Jörn. 2012. “Transcriptional regulation of the operon encoding stress-responsive ECF sigma factor SigH and its anti-sigma factor RshA, and control of its regulatory network in Corynebacterium glutamicum”. BMC Genomics 13 (1): 445.
Busche, T., Silar, R., Picmanova, M., Patek, M., and Kalinowski, J. (2012). Transcriptional regulation of the operon encoding stress-responsive ECF sigma factor SigH and its anti-sigma factor RshA, and control of its regulatory network in Corynebacterium glutamicum. BMC Genomics 13:445.
Busche, T., et al., 2012. Transcriptional regulation of the operon encoding stress-responsive ECF sigma factor SigH and its anti-sigma factor RshA, and control of its regulatory network in Corynebacterium glutamicum. BMC Genomics, 13(1): 445.
T. Busche, et al., “Transcriptional regulation of the operon encoding stress-responsive ECF sigma factor SigH and its anti-sigma factor RshA, and control of its regulatory network in Corynebacterium glutamicum”, BMC Genomics, vol. 13, 2012, : 445.
Busche, T., Silar, R., Picmanova, M., Patek, M., Kalinowski, J.: Transcriptional regulation of the operon encoding stress-responsive ECF sigma factor SigH and its anti-sigma factor RshA, and control of its regulatory network in Corynebacterium glutamicum. BMC Genomics. 13, : 445 (2012).
Busche, Tobias, Silar, Radoslav, Picmanova, Martina, Patek, Miroslav, and Kalinowski, Jörn. “Transcriptional regulation of the operon encoding stress-responsive ECF sigma factor SigH and its anti-sigma factor RshA, and control of its regulatory network in Corynebacterium glutamicum”. BMC Genomics 13.1 (2012): 445.
Alle Dateien verfügbar unter der/den folgenden Lizenz(en):
Copyright Statement:
Dieses Objekt ist durch das Urheberrecht und/oder verwandte Schutzrechte geschützt. [...]
Volltext(e)
Access Level
Open Access
Zuletzt Hochgeladen
2019-09-06T09:18:06Z
MD5 Prüfsumme
b16f998c5bd1e4a5f60ed13cdc73e16f
31 Zitationen in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
Function of alkyl hydroperoxidase AhpD in resistance to oxidative stress in Corynebacterium glutamicum.
Su T, Si M, Zhao Y, Yao S, Che C, Liu Y, Chen C., J Gen Appl Microbiol 65(2), 2019
PMID: 30249939
Su T, Si M, Zhao Y, Yao S, Che C, Liu Y, Chen C., J Gen Appl Microbiol 65(2), 2019
PMID: 30249939
Stable integration of the Mrx1-roGFP2 biosensor to monitor dynamic changes of the mycothiol redox potential in Corynebacterium glutamicum.
Tung QN, Loi VV, Busche T, Nerlich A, Mieth M, Milse J, Kalinowski J, Hocke AC, Antelmann H., Redox Biol 20(), 2019
PMID: 30481728
Tung QN, Loi VV, Busche T, Nerlich A, Mieth M, Milse J, Kalinowski J, Hocke AC, Antelmann H., Redox Biol 20(), 2019
PMID: 30481728
Identifying the Growth Modulon of Corynebacterium glutamicum.
Haas T, Graf M, Nieß A, Busche T, Kalinowski J, Blombach B, Takors R., Front Microbiol 10(), 2019
PMID: 31134020
Haas T, Graf M, Nieß A, Busche T, Kalinowski J, Blombach B, Takors R., Front Microbiol 10(), 2019
PMID: 31134020
Chemistry and Redox Biology of Mycothiol.
Reyes AM, Pedre B, De Armas MI, Tossounian MA, Radi R, Messens J, Trujillo M., Antioxid Redox Signal 28(6), 2018
PMID: 28372502
Reyes AM, Pedre B, De Armas MI, Tossounian MA, Radi R, Messens J, Trujillo M., Antioxid Redox Signal 28(6), 2018
PMID: 28372502
Differential transcriptomic analysis reveals hidden light response in Streptomyces lividans.
Koepff J, Morschett H, Busche T, Winkler A, Kalinowski J, Wiechert W, Oldiges M., Biotechnol Prog 34(1), 2018
PMID: 28960883
Koepff J, Morschett H, Busche T, Winkler A, Kalinowski J, Wiechert W, Oldiges M., Biotechnol Prog 34(1), 2018
PMID: 28960883
Extracytoplasmic function sigma factor σD confers resistance to environmental stress by enhancing mycolate synthesis and modifying peptidoglycan structures in Corynebacterium glutamicum.
Toyoda K, Inui M., Mol Microbiol 107(3), 2018
PMID: 29148103
Toyoda K, Inui M., Mol Microbiol 107(3), 2018
PMID: 29148103
Overlap of Promoter Recognition Specificity of Stress Response Sigma Factors SigD and SigH in Corynebacterium glutamicum ATCC 13032.
Dostálová H, Busche T, Holátko J, Rucká L, Štěpánek V, Barvík I, Nešvera J, Kalinowski J, Pátek M., Front Microbiol 9(), 2018
PMID: 30687273
Dostálová H, Busche T, Holátko J, Rucká L, Štěpánek V, Barvík I, Nešvera J, Kalinowski J, Pátek M., Front Microbiol 9(), 2018
PMID: 30687273
A thioredoxin-dependent peroxiredoxin Q from Corynebacterium glutamicum plays an important role in defense against oxidative stress.
Su T, Si M, Zhao Y, Liu Y, Yao S, Che C, Chen C., PLoS One 13(2), 2018
PMID: 29438446
Su T, Si M, Zhao Y, Liu Y, Yao S, Che C, Chen C., PLoS One 13(2), 2018
PMID: 29438446
Multi-Omics and Targeted Approaches to Determine the Role of Cellular Proteases in Streptomyces Protein Secretion.
Busche T, Tsolis KC, Koepff J, Rebets Y, Rückert C, Hamed MB, Bleidt A, Wiechert W, Lopatniuk M, Yousra A, Anné J, Karamanou S, Oldiges M, Kalinowski J, Luzhetskyy A, Economou A., Front Microbiol 9(), 2018
PMID: 29915569
Busche T, Tsolis KC, Koepff J, Rebets Y, Rückert C, Hamed MB, Bleidt A, Wiechert W, Lopatniuk M, Yousra A, Anné J, Karamanou S, Oldiges M, Kalinowski J, Luzhetskyy A, Economou A., Front Microbiol 9(), 2018
PMID: 29915569
Characterization of Sigma Factor Genes in Streptomyces lividans TK24 Using a Genomic Library-Based Approach for Multiple Gene Deletions.
Rebets Y, Tsolis KC, Guðmundsdóttir EE, Koepff J, Wawiernia B, Busche T, Bleidt A, Horbal L, Myronovskyi M, Ahmed Y, Wiechert W, Rückert C, Hamed MB, Bilyk B, Anné J, Friðjónsson Ó, Kalinowski J, Oldiges M, Economou A, Luzhetskyy A., Front Microbiol 9(), 2018
PMID: 30619125
Rebets Y, Tsolis KC, Guðmundsdóttir EE, Koepff J, Wawiernia B, Busche T, Bleidt A, Horbal L, Myronovskyi M, Ahmed Y, Wiechert W, Rückert C, Hamed MB, Bilyk B, Anné J, Friðjónsson Ó, Kalinowski J, Oldiges M, Economou A, Luzhetskyy A., Front Microbiol 9(), 2018
PMID: 30619125
Fast and reliable strain characterization of Streptomyces lividans through micro-scale cultivation.
Koepff J, Keller M, Tsolis KC, Busche T, Rückert C, Hamed MB, Anné J, Kalinowski J, Wiechert W, Economou A, Oldiges M., Biotechnol Bioeng 114(9), 2017
PMID: 28436005
Koepff J, Keller M, Tsolis KC, Busche T, Rückert C, Hamed MB, Anné J, Kalinowski J, Wiechert W, Economou A, Oldiges M., Biotechnol Bioeng 114(9), 2017
PMID: 28436005
Assignment of sigma factors of RNA polymerase to promoters in Corynebacterium glutamicum.
Dostálová H, Holátko J, Busche T, Rucká L, Rapoport A, Halada P, Nešvera J, Kalinowski J, Pátek M., AMB Express 7(1), 2017
PMID: 28651382
Dostálová H, Holátko J, Busche T, Rucká L, Rapoport A, Halada P, Nešvera J, Kalinowski J, Pátek M., AMB Express 7(1), 2017
PMID: 28651382
Physiological roles of sigma factor SigD in Corynebacterium glutamicum.
Taniguchi H, Busche T, Patschkowski T, Niehaus K, Pátek M, Kalinowski J, Wendisch VF., BMC Microbiol 17(1), 2017
PMID: 28701150
Taniguchi H, Busche T, Patschkowski T, Niehaus K, Pátek M, Kalinowski J, Wendisch VF., BMC Microbiol 17(1), 2017
PMID: 28701150
Identification of a suitable promoter for the sigma factor of Mycobacterium tuberculosis.
Mallick Gupta A, Mukherjee S, Dutta A, Mukhopadhyay J, Bhattacharyya D, Mandal S., Mol Biosyst 13(11), 2017
PMID: 28952652
Mallick Gupta A, Mukherjee S, Dutta A, Mukhopadhyay J, Bhattacharyya D, Mandal S., Mol Biosyst 13(11), 2017
PMID: 28952652
Expression of recombinant protein using Corynebacterium Glutamicum: progress, challenges and applications.
Liu X, Yang Y, Zhang W, Sun Y, Peng F, Jeffrey L, Harvey L, McNeil B, Bai Z., Crit Rev Biotechnol 36(4), 2016
PMID: 25714007
Liu X, Yang Y, Zhang W, Sun Y, Peng F, Jeffrey L, Harvey L, McNeil B, Bai Z., Crit Rev Biotechnol 36(4), 2016
PMID: 25714007
Regulons of global transcription factors in Corynebacterium glutamicum.
Toyoda K, Inui M., Appl Microbiol Biotechnol 100(1), 2016
PMID: 26496920
Toyoda K, Inui M., Appl Microbiol Biotechnol 100(1), 2016
PMID: 26496920
The extracytoplasmic function σ factor σ(C) regulates expression of a branched quinol oxidation pathway in Corynebacterium glutamicum.
Toyoda K, Inui M., Mol Microbiol 100(3), 2016
PMID: 26789738
Toyoda K, Inui M., Mol Microbiol 100(3), 2016
PMID: 26789738
Use of In Vitro Transcription System for Analysis of Corynebacterium glutamicum Promoters Recognized by Two Sigma Factors.
Šilar R, Holátko J, Rucká L, Rapoport A, Dostálová H, Kadeřábková P, Nešvera J, Pátek M., Curr Microbiol 73(3), 2016
PMID: 27270733
Šilar R, Holátko J, Rucká L, Rapoport A, Dostálová H, Kadeřábková P, Nešvera J, Pátek M., Curr Microbiol 73(3), 2016
PMID: 27270733
Overexpression of Mycothiol Disulfide Reductase Enhances Corynebacterium glutamicum Robustness by Modulating Cellular Redox Homeostasis and Antioxidant Proteins under Oxidative Stress.
Si M, Zhao C, Zhang B, Wei D, Chen K, Yang X, Xiao H, Shen X., Sci Rep 6(), 2016
PMID: 27383057
Si M, Zhao C, Zhang B, Wei D, Chen K, Yang X, Xiao H, Shen X., Sci Rep 6(), 2016
PMID: 27383057
Expanding the regulatory network governed by the extracytoplasmic function sigma factor σH in Corynebacterium glutamicum.
Toyoda K, Teramoto H, Yukawa H, Inui M., J Bacteriol 197(3), 2015
PMID: 25404703
Toyoda K, Teramoto H, Yukawa H, Inui M., J Bacteriol 197(3), 2015
PMID: 25404703
Corynebacterium glutamicum methionine sulfoxide reductase A uses both mycoredoxin and thioredoxin for regeneration and oxidative stress resistance.
Si M, Zhang L, Chaudhry MT, Ding W, Xu Y, Chen C, Akbar A, Shen X, Liu SJ., Appl Environ Microbiol 81(8), 2015
PMID: 25681179
Si M, Zhang L, Chaudhry MT, Ding W, Xu Y, Chen C, Akbar A, Shen X, Liu SJ., Appl Environ Microbiol 81(8), 2015
PMID: 25681179
Redox regulation by reversible protein S-thiolation in bacteria.
Loi VV, Rossius M, Antelmann H., Front Microbiol 6(), 2015
PMID: 25852656
Loi VV, Rossius M, Antelmann H., Front Microbiol 6(), 2015
PMID: 25852656
Thiol-based redox switches in prokaryotes.
Hillion M, Antelmann H., Biol Chem 396(5), 2015
PMID: 25720121
Hillion M, Antelmann H., Biol Chem 396(5), 2015
PMID: 25720121
Exploring the role of sigma factor gene expression on production by Corynebacterium glutamicum: sigma factor H and FMN as example.
Taniguchi H, Wendisch VF., Front Microbiol 6(), 2015
PMID: 26257719
Taniguchi H, Wendisch VF., Front Microbiol 6(), 2015
PMID: 26257719
Protein S-mycothiolation functions as redox-switch and thiol protection mechanism in Corynebacterium glutamicum under hypochlorite stress.
Chi BK, Busche T, Van Laer K, Bäsell K, Becher D, Clermont L, Seibold GM, Persicke M, Kalinowski J, Messens J, Antelmann H., Antioxid Redox Signal 20(4), 2014
PMID: 23886307
Chi BK, Busche T, Van Laer K, Bäsell K, Becher D, Clermont L, Seibold GM, Persicke M, Kalinowski J, Messens J, Antelmann H., Antioxid Redox Signal 20(4), 2014
PMID: 23886307
Transcriptional response of Corynebacterium glutamicum ATCC 13032 to hydrogen peroxide stress and characterization of the OxyR regulon.
Milse J, Petri K, Rückert C, Kalinowski J., J Biotechnol 190(), 2014
PMID: 25107507
Milse J, Petri K, Rückert C, Kalinowski J., J Biotechnol 190(), 2014
PMID: 25107507
Functional characterization of Corynebacterium glutamicum mycothiol S-conjugate amidase.
Si M, Long M, Chaudhry MT, Xu Y, Zhang P, Zhang L, Shen X., PLoS One 9(12), 2014
PMID: 25514023
Si M, Long M, Chaudhry MT, Xu Y, Zhang P, Zhang L, Shen X., PLoS One 9(12), 2014
PMID: 25514023
Transcriptional control of the F0F1-ATP synthase operon of Corynebacterium glutamicum: SigmaH factor binds to its promoter and regulates its expression at different pH values.
Barriuso-Iglesias M, Barreiro C, Sola-Landa A, Martín JF., Microb Biotechnol 6(2), 2013
PMID: 23298179
Barriuso-Iglesias M, Barreiro C, Sola-Landa A, Martín JF., Microb Biotechnol 6(2), 2013
PMID: 23298179
Corynebacterium glutamicum promoters: a practical approach.
Pátek M, Holátko J, Busche T, Kalinowski J, Nešvera J., Microb Biotechnol 6(2), 2013
PMID: 23305350
Pátek M, Holátko J, Busche T, Kalinowski J, Nešvera J., Microb Biotechnol 6(2), 2013
PMID: 23305350
Key role for the alternative sigma factor, SigH, in the intracellular life of Mycobacterium avium subsp. paratuberculosis during macrophage stress.
Ghosh P, Wu CW, Talaat AM., Infect Immun 81(6), 2013
PMID: 23569115
Ghosh P, Wu CW, Talaat AM., Infect Immun 81(6), 2013
PMID: 23569115
Comprehensive discovery and characterization of small RNAs in Corynebacterium glutamicum ATCC 13032.
Mentz A, Neshat A, Pfeifer-Sancar K, Pühler A, Rückert C, Kalinowski J., BMC Genomics 14(), 2013
PMID: 24138339
Mentz A, Neshat A, Pfeifer-Sancar K, Pühler A, Rückert C, Kalinowski J., BMC Genomics 14(), 2013
PMID: 24138339
60 References
Daten bereitgestellt von Europe PubMed Central.
The individual and common repertoire of DNA-binding transcriptional regulators of Corynebacterium glutamicum, Corynebacterium efficiens, Corynebacterium diphtheriae and Corynebacterium jeikeium deduced from the complete genome sequences.
Brune I, Brinkrolf K, Kalinowski J, Puhler A, Tauch A., BMC Genomics 6(), 2005
PMID: 15938759
Brune I, Brinkrolf K, Kalinowski J, Puhler A, Tauch A., BMC Genomics 6(), 2005
PMID: 15938759
Identification of an alpha(1-->6) mannopyranosyltransferase (MptA), involved in Corynebacterium glutamicum lipomanann biosynthesis, and identification of its orthologue in Mycobacterium tuberculosis.
Mishra AK, Alderwick LJ, Rittmann D, Tatituri RV, Nigou J, Gilleron M, Eggeling L, Besra GS., Mol. Microbiol. 65(6), 2007
PMID: 17714444
Mishra AK, Alderwick LJ, Rittmann D, Tatituri RV, Nigou J, Gilleron M, Eggeling L, Besra GS., Mol. Microbiol. 65(6), 2007
PMID: 17714444
Deletion of the genes encoding the MtrA-MtrB two-component system of Corynebacterium glutamicum has a strong influence on cell morphology, antibiotics susceptibility and expression of genes involved in osmoprotection.
Moker N, Brocker M, Schaffer S, Kramer R, Morbach S, Bott M., Mol. Microbiol. 54(2), 2004
PMID: 15469514
Moker N, Brocker M, Schaffer S, Kramer R, Morbach S, Bott M., Mol. Microbiol. 54(2), 2004
PMID: 15469514
The Corynebacterium glutamicum genome: features and impacts on biotechnological processes.
Ikeda M, Nakagawa S., Appl. Microbiol. Biotechnol. 62(2-3), 2003
PMID: 12743753
Ikeda M, Nakagawa S., Appl. Microbiol. Biotechnol. 62(2-3), 2003
PMID: 12743753
The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins.
Kalinowski J, Bathe B, Bartels D, Bischoff N, Bott M, Burkovski A, Dusch N, Eggeling L, Eikmanns BJ, Gaigalat L, Goesmann A, Hartmann M, Huthmacher K, Kramer R, Linke B, McHardy AC, Meyer F, Mockel B, Pfefferle W, Puhler A, Rey DA, Ruckert C, Rupp O, Sahm H, Wendisch VF, Wiegrabe I, Tauch A., J. Biotechnol. 104(1-3), 2003
PMID: 12948626
Kalinowski J, Bathe B, Bartels D, Bischoff N, Bott M, Burkovski A, Dusch N, Eggeling L, Eikmanns BJ, Gaigalat L, Goesmann A, Hartmann M, Huthmacher K, Kramer R, Linke B, McHardy AC, Meyer F, Mockel B, Pfefferle W, Puhler A, Rey DA, Ruckert C, Rupp O, Sahm H, Wendisch VF, Wiegrabe I, Tauch A., J. Biotechnol. 104(1-3), 2003
PMID: 12948626
Comparative analysis of the Corynebacterium glutamicum group and complete genome sequence of strain R.
Yukawa H, Omumasaba CA, Nonaka H, Kos P, Okai N, Suzuki N, Suda M, Tsuge Y, Watanabe J, Ikeda Y, Vertes AA, Inui M., Microbiology (Reading, Engl.) 153(Pt 4), 2007
PMID: 17379713
Yukawa H, Omumasaba CA, Nonaka H, Kos P, Okai N, Suzuki N, Suda M, Tsuge Y, Watanabe J, Ikeda Y, Vertes AA, Inui M., Microbiology (Reading, Engl.) 153(Pt 4), 2007
PMID: 17379713
Multiple sigma subunits and the partitioning of bacterial transcription space.
Gruber TM, Gross CA., Annu. Rev. Microbiol. 57(), 2003
PMID: 14527287
Gruber TM, Gross CA., Annu. Rev. Microbiol. 57(), 2003
PMID: 14527287
Sigma factors and promoters in Corynebacterium glutamicum.
Patek M, Nesvera J., J. Biotechnol. 154(2-3), 2011
PMID: 21277915
Patek M, Nesvera J., J. Biotechnol. 154(2-3), 2011
PMID: 21277915
The alternative sigma factor SigB of Corynebacterium glutamicum modulates global gene expression during transition from exponential growth to stationary phase
AUTHOR UNKNOWN, 2007
AUTHOR UNKNOWN, 2007
Corynebacterium glutamicum sigmaE is involved in responses to cell surface stresses and its activity is controlled by the anti-sigma factor CseE.
Park SD, Youn JW, Kim YJ, Lee SM, Kim Y, Lee HS., Microbiology (Reading, Engl.) 154(Pt 3), 2008
PMID: 18310037
Park SD, Youn JW, Kim YJ, Lee SM, Kim Y, Lee HS., Microbiology (Reading, Engl.) 154(Pt 3), 2008
PMID: 18310037
RsrA, an anti-sigma factor regulated by redox change.
Kang JG, Paget MS, Seok YJ, Hahn MY, Bae JB, Hahn JS, Kleanthous C, Buttner MJ, Roe JH., EMBO J. 18(15), 1999
PMID: 10428967
Kang JG, Paget MS, Seok YJ, Hahn MY, Bae JB, Hahn JS, Kleanthous C, Buttner MJ, Roe JH., EMBO J. 18(15), 1999
PMID: 10428967
RshA, an anti-sigma factor that regulates the activity of the mycobacterial stress response sigma factor SigH.
Song T, Dove SL, Lee KH, Husson RN., Mol. Microbiol. 50(3), 2003
PMID: 14617153
Song T, Dove SL, Lee KH, Husson RN., Mol. Microbiol. 50(3), 2003
PMID: 14617153
Determinants of redox sensitivity in RsrA, a zinc-containing anti-sigma factor for regulating thiol oxidative stress response.
Jung YG, Cho YB, Kim MS, Yoo JS, Hong SH, Roe JH., Nucleic Acids Res. 39(17), 2011
PMID: 21685450
Jung YG, Cho YB, Kim MS, Yoo JS, Hong SH, Roe JH., Nucleic Acids Res. 39(17), 2011
PMID: 21685450
Regulation of Corynebacterium glutamicum heat shock response by the extracytoplasmic-function sigma factor SigH and transcriptional regulators HspR and HrcA.
Ehira S, Teramoto H, Inui M, Yukawa H., J. Bacteriol. 191(9), 2009
PMID: 19270092
Ehira S, Teramoto H, Inui M, Yukawa H., J. Bacteriol. 191(9), 2009
PMID: 19270092
Functional analysis of sigH expression in Corynebacterium glutamicum.
Kim TH, Kim HJ, Park JS, Kim Y, Kim P, Lee HS., Biochem. Biophys. Res. Commun. 331(4), 2005
PMID: 15883048
Kim TH, Kim HJ, Park JS, Kim Y, Kim P, Lee HS., Biochem. Biophys. Res. Commun. 331(4), 2005
PMID: 15883048
clpC and clpP1P2 gene expression in Corynebacterium glutamicum is controlled by a regulatory network involving the transcriptional regulators ClgR and HspR as well as the ECF sigma factor sigmaH.
Engels S, Schweitzer JE, Ludwig C, Bott M, Schaffer S., Mol. Microbiol. 52(1), 2004
PMID: 15049827
Engels S, Schweitzer JE, Ludwig C, Bott M, Schaffer S., Mol. Microbiol. 52(1), 2004
PMID: 15049827
Transcriptional analysis of the groES-groEL1, groEL2, and dnaK genes in Corynebacterium glutamicum: characterization of heat shock-induced promoters.
Barreiro C, Gonzalez-Lavado E, Patek M, Martin JF., J. Bacteriol. 186(14), 2004
PMID: 15231814
Barreiro C, Gonzalez-Lavado E, Patek M, Martin JF., J. Bacteriol. 186(14), 2004
PMID: 15231814
Microarray studies reveal a 'differential response' to moderate or severe heat shock of the HrcA- and HspR-dependent systems in Corynebacterium glutamicum.
Barreiro C, Nakunst D, Huser AT, de Paz HD, Kalinowski J, Martin JF., Microbiology (Reading, Engl.) 155(Pt 2), 2009
PMID: 19202085
Barreiro C, Nakunst D, Huser AT, de Paz HD, Kalinowski J, Martin JF., Microbiology (Reading, Engl.) 155(Pt 2), 2009
PMID: 19202085
Redox control in actinobacteria.
den Hengst CD, Buttner MJ., Biochim. Biophys. Acta 1780(11), 2008
PMID: 18252205
den Hengst CD, Buttner MJ., Biochim. Biophys. Acta 1780(11), 2008
PMID: 18252205
The whcA gene plays a negative role in oxidative stress response of Corynebacterium glutamicum.
Choi WW, Park SD, Lee SM, Kim HB, Kim Y, Lee HS., FEMS Microbiol. Lett. 290(1), 2008
PMID: 19016879
Choi WW, Park SD, Lee SM, Kim HB, Kim Y, Lee HS., FEMS Microbiol. Lett. 290(1), 2008
PMID: 19016879
The whcE gene of Corynebacterium glutamicum is important for survival following heat and oxidative stress.
Kim TH, Park JS, Kim HJ, Kim Y, Kim P, Lee HS., Biochem. Biophys. Res. Commun. 337(3), 2005
PMID: 16212936
Kim TH, Park JS, Kim HJ, Kim Y, Kim P, Lee HS., Biochem. Biophys. Res. Commun. 337(3), 2005
PMID: 16212936
Cloning and transcriptional characterization of two sigma factor genes, sigA and sigB, from Brevibacterium flavum.
Halgasova N, Bukovska G, Timko J, Kormanec J., Curr. Microbiol. 43(4), 2001
PMID: 11683358
Halgasova N, Bukovska G, Timko J, Kormanec J., Curr. Microbiol. 43(4), 2001
PMID: 11683358
Group 2 sigma factor SigB of Corynebacterium glutamicum positively regulates glucose metabolism under conditions of oxygen deprivation.
Ehira S, Shirai T, Teramoto H, Inui M, Yukawa H., Appl. Environ. Microbiol. 74(16), 2008
PMID: 18567683
Ehira S, Shirai T, Teramoto H, Inui M, Yukawa H., Appl. Environ. Microbiol. 74(16), 2008
PMID: 18567683
The extracytoplasmic function-type sigma factor SigM of Corynebacterium glutamicum ATCC 13032 is involved in transcription of disulfide stress-related genes.
Nakunst D, Larisch C, Huser AT, Tauch A, Puhler A, Kalinowski J., J. Bacteriol. 189(13), 2007
PMID: 17483229
Nakunst D, Larisch C, Huser AT, Tauch A, Puhler A, Kalinowski J., J. Bacteriol. 189(13), 2007
PMID: 17483229
Positive and negative feedback regulatory loops of thiol-oxidative stress response mediated by an unstable isoform of sigmaR in actinomycetes.
Kim MS, Hahn MY, Cho Y, Cho SN, Roe JH., Mol. Microbiol. 73(5), 2009
PMID: 19682253
Kim MS, Hahn MY, Cho Y, Cho SN, Roe JH., Mol. Microbiol. 73(5), 2009
PMID: 19682253
Complete genome sequence and analysis of the multiresistant nosocomial pathogen Corynebacterium jeikeium K411, a lipid-requiring bacterium of the human skin flora.
Tauch A, Kaiser O, Hain T, Goesmann A, Weisshaar B, Albersmeier A, Bekel T, Bischoff N, Brune I, Chakraborty T, Kalinowski J, Meyer F, Rupp O, Schneiker S, Viehoever P, Puhler A., J. Bacteriol. 187(13), 2005
PMID: 15968079
Tauch A, Kaiser O, Hain T, Goesmann A, Weisshaar B, Albersmeier A, Bekel T, Bischoff N, Brune I, Chakraborty T, Kalinowski J, Meyer F, Rupp O, Schneiker S, Viehoever P, Puhler A., J. Bacteriol. 187(13), 2005
PMID: 15968079
Genetic makeup of the Corynebacterium glutamicum LexA regulon deduced from comparative transcriptomics and in vitro DNA band shift assays.
Jochmann N, Kurze AK, Czaja LF, Brinkrolf K, Brune I, Huser AT, Hansmeier N, Puhler A, Borovok I, Tauch A., Microbiology (Reading, Engl.) 155(Pt 5), 2009
PMID: 19372162
Jochmann N, Kurze AK, Czaja LF, Brinkrolf K, Brune I, Huser AT, Hansmeier N, Puhler A, Borovok I, Tauch A., Microbiology (Reading, Engl.) 155(Pt 5), 2009
PMID: 19372162
The alternative sigma factor SigH regulates major components of oxidative and heat stress responses in Mycobacterium tuberculosis.
Raman S, Song T, Puyang X, Bardarov S, Jacobs WR Jr, Husson RN., J. Bacteriol. 183(20), 2001
PMID: 11567012
Raman S, Song T, Puyang X, Bardarov S, Jacobs WR Jr, Husson RN., J. Bacteriol. 183(20), 2001
PMID: 11567012
Defining the disulphide stress response in Streptomyces coelicolor A3(2): identification of the sigmaR regulon.
Paget MS, Molle V, Cohen G, Aharonowitz Y, Buttner MJ., Mol. Microbiol. 42(4), 2001
PMID: 11737643
Paget MS, Molle V, Cohen G, Aharonowitz Y, Buttner MJ., Mol. Microbiol. 42(4), 2001
PMID: 11737643
Regulation of mycothiol metabolism by sigma(R) and the thiol redox sensor anti-sigma factor RsrA.
Newton GL, Fahey RC., Mol. Microbiol. 68(4), 2008
PMID: 18430078
Newton GL, Fahey RC., Mol. Microbiol. 68(4), 2008
PMID: 18430078
Biosynthesis and functions of mycothiol, the unique protective thiol of Actinobacteria.
Newton GL, Buchmeier N, Fahey RC., Microbiol. Mol. Biol. Rev. 72(3), 2008
PMID: 18772286
Newton GL, Buchmeier N, Fahey RC., Microbiol. Mol. Biol. Rev. 72(3), 2008
PMID: 18772286
Proteasomal protein degradation in Mycobacteria is dependent upon a prokaryotic ubiquitin-like protein.
Burns KE, Liu WT, Boshoff HI, Dorrestein PC, Barry CE 3rd., J. Biol. Chem. 284(5), 2008
PMID: 19028679
Burns KE, Liu WT, Boshoff HI, Dorrestein PC, Barry CE 3rd., J. Biol. Chem. 284(5), 2008
PMID: 19028679
Chromosomally encoded small antisense RNA in Corynebacterium glutamicum.
Zemanova M, Kaderabkova P, Patek M, Knoppova M, Silar R, Nesvera J., FEMS Microbiol. Lett. 279(2), 2007
PMID: 18093135
Zemanova M, Kaderabkova P, Patek M, Knoppova M, Silar R, Nesvera J., FEMS Microbiol. Lett. 279(2), 2007
PMID: 18093135
Development of a Corynebacterium glutamicum DNA microarray and validation by genome-wide expression profiling during growth with propionate as carbon source.
Huser AT, Becker A, Brune I, Dondrup M, Kalinowski J, Plassmeier J, Puhler A, Wiegrabe I, Tauch A., J. Biotechnol. 106(2-3), 2003
PMID: 14651867
Huser AT, Becker A, Brune I, Dondrup M, Kalinowski J, Plassmeier J, Puhler A, Wiegrabe I, Tauch A., J. Biotechnol. 106(2-3), 2003
PMID: 14651867
BioProspector: discovering conserved DNA motifs in upstream regulatory regions of co-expressed genes.
Liu X, Brutlag DL, Liu JS., Pac Symp Biocomput (), 2001
PMID: 11262934
Liu X, Brutlag DL, Liu JS., Pac Symp Biocomput (), 2001
PMID: 11262934
Distribution of thiols in microorganisms: mycothiol is a major thiol in most actinomycetes.
Newton GL, Arnold K, Price MS, Sherrill C, Delcardayre SB, Aharonowitz Y, Cohen G, Davies J, Fahey RC, Davis C., J. Bacteriol. 178(7), 1996
PMID: 8606174
Newton GL, Arnold K, Price MS, Sherrill C, Delcardayre SB, Aharonowitz Y, Cohen G, Davies J, Fahey RC, Davis C., J. Bacteriol. 178(7), 1996
PMID: 8606174
The gene ncgl2918 encodes a novel maleylpyruvate isomerase that needs mycothiol as cofactor and links mycothiol biosynthesis and gentisate assimilation in Corynebacterium glutamicum.
Feng J, Che Y, Milse J, Yin YJ, Liu L, Ruckert C, Shen XH, Qi SW, Kalinowski J, Liu SJ., J. Biol. Chem. 281(16), 2006
PMID: 16481315
Feng J, Che Y, Milse J, Yin YJ, Liu L, Ruckert C, Shen XH, Qi SW, Kalinowski J, Liu SJ., J. Biol. Chem. 281(16), 2006
PMID: 16481315
Mycothiol regulates and is regulated by a thiol-specific antisigma factor RsrA and sigma(R) in Streptomyces coelicolor.
Park JH, Roe JH., Mol. Microbiol. 68(4), 2008
PMID: 18430082
Park JH, Roe JH., Mol. Microbiol. 68(4), 2008
PMID: 18430082
The SOS response of Escherichia coli
AUTHOR UNKNOWN, 1996
AUTHOR UNKNOWN, 1996
AUTHOR UNKNOWN, 2005
DNA binding properties of the LexA repressor.
Schnarr M, Oertel-Buchheit P, Kazmaier M, Granger-Schnarr M., Biochimie 73(4), 1991
PMID: 1911942
Schnarr M, Oertel-Buchheit P, Kazmaier M, Granger-Schnarr M., Biochimie 73(4), 1991
PMID: 1911942
AUTHOR UNKNOWN, 1989
Isoleucine synthesis in Corynebacterium glutamicum: molecular analysis of the ilvB-ilvN-ilvC operon.
Keilhauer C, Eggeling L, Sahm H., J. Bacteriol. 175(17), 1993
PMID: 8366043
Keilhauer C, Eggeling L, Sahm H., J. Bacteriol. 175(17), 1993
PMID: 8366043
The Corynebacterium xerosis composite transposon Tn5432 consists of two identical insertion sequences, designated IS1249, flanking the erythromycin resistance gene ermCX.
Tauch A, Kassing F, Kalinowski J, Puhler A., Plasmid 34(2), 1995
PMID: 8559800
Tauch A, Kassing F, Kalinowski J, Puhler A., Plasmid 34(2), 1995
PMID: 8559800
Techniques for transformation of E. coli
AUTHOR UNKNOWN, 1985
AUTHOR UNKNOWN, 1985
Corynebacterium glutamicum DNA is subjected to methylation-restriction in Escherichia coli.
Tauch A, Kirchner O, Wehmeier L, Kalinowski J, Puhler A., FEMS Microbiol. Lett. 123(3), 1994
PMID: 7988915
Tauch A, Kirchner O, Wehmeier L, Kalinowski J, Puhler A., FEMS Microbiol. Lett. 123(3), 1994
PMID: 7988915
Efficient electrotransformation of corynebacterium diphtheriae with a mini-replicon derived from the Corynebacterium glutamicum plasmid pGA1.
Tauch A, Kirchner O, Loffler B, Gotker S, Puhler A, Kalinowski J., Curr. Microbiol. 45(5), 2002
PMID: 12232668
Tauch A, Kirchner O, Loffler B, Gotker S, Puhler A, Kalinowski J., Curr. Microbiol. 45(5), 2002
PMID: 12232668
Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension.
Horton RM, Hunt HD, Ho SN, Pullen JK, Pease LR., Gene 77(1), 1989
PMID: 2744488
Horton RM, Hunt HD, Ho SN, Pullen JK, Pease LR., Gene 77(1), 1989
PMID: 2744488
Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum.
Schafer A, Tauch A, Jager W, Kalinowski J, Thierbach G, Puhler A., Gene 145(1), 1994
PMID: 8045426
Schafer A, Tauch A, Jager W, Kalinowski J, Thierbach G, Puhler A., Gene 145(1), 1994
PMID: 8045426
Genome-wide analysis of the L-methionine biosynthetic pathway in Corynebacterium glutamicum by targeted gene deletion and homologous complementation.
Ruckert C, Puhler A, Kalinowski J., J. Biotechnol. 104(1-3), 2003
PMID: 12948640
Ruckert C, Puhler A, Kalinowski J., J. Biotechnol. 104(1-3), 2003
PMID: 12948640
The dual transcriptional regulator CysR in Corynebacterium glutamicum ATCC 13032 controls a subset of genes of the McbR regulon in response to the availability of sulphide acceptor molecules.
Ruckert C, Milse J, Albersmeier A, Koch DJ, Puhler A, Kalinowski J., BMC Genomics 9(), 2008
PMID: 18854009
Ruckert C, Milse J, Albersmeier A, Koch DJ, Puhler A, Kalinowski J., BMC Genomics 9(), 2008
PMID: 18854009
EMMA 2--a MAGE-compliant system for the collaborative analysis and integration of microarray data.
Dondrup M, Albaum SP, Griebel T, Henckel K, Junemann S, Kahlke T, Kleindt CK, Kuster H, Linke B, Mertens D, Mittard-Runte V, Neuweger H, Runte KJ, Tauch A, Tille F, Puhler A, Goesmann A., BMC Bioinformatics 10(), 2009
PMID: 19200358
Dondrup M, Albaum SP, Griebel T, Henckel K, Junemann S, Kahlke T, Kleindt CK, Kuster H, Linke B, Mertens D, Mittard-Runte V, Neuweger H, Runte KJ, Tauch A, Tille F, Puhler A, Goesmann A., BMC Bioinformatics 10(), 2009
PMID: 19200358
The genes of lepA and hemN form a bicistronic operon in Bacillus subtilis.
Homuth G, Heinemann M, Zuber U, Schumann W., Microbiology (Reading, Engl.) 142 ( Pt 7)(), 1996
PMID: 8757728
Homuth G, Heinemann M, Zuber U, Schumann W., Microbiology (Reading, Engl.) 142 ( Pt 7)(), 1996
PMID: 8757728
Function of Corynebacterium glutamicum promoters in Escherichia coli, Streptomyces lividans, and Bacillus subtilis.
Patek M, Muth G, Wohlleben W., J. Biotechnol. 104(1-3), 2003
PMID: 12948649
Patek M, Muth G, Wohlleben W., J. Biotechnol. 104(1-3), 2003
PMID: 12948649
Metabolic engineering of the L-valine biosynthesis pathway in Corynebacterium glutamicum using promoter activity modulation.
Holatko J, Elisakova V, Prouza M, Sobotka M, Nesvera J, Patek M., J. Biotechnol. 139(3), 2008
PMID: 19121344
Holatko J, Elisakova V, Prouza M, Sobotka M, Nesvera J, Patek M., J. Biotechnol. 139(3), 2008
PMID: 19121344
Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria.
Shaw WV., Meth. Enzymol. 43(), 1975
PMID: 1094240
Shaw WV., Meth. Enzymol. 43(), 1975
PMID: 1094240
WebLogo: a sequence logo generator.
Crooks GE, Hon G, Chandonia JM, Brenner SE., Genome Res. 14(6), 2004
PMID: 15173120
Crooks GE, Hon G, Chandonia JM, Brenner SE., Genome Res. 14(6), 2004
PMID: 15173120
Integrative and autonomously replicating vectors for analysis of promoters in Corynebacterium glutamicum
AUTHOR UNKNOWN, 1998
AUTHOR UNKNOWN, 1998
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
Yanisch-Perron C, Vieira J, Messing J., Gene 33(1), 1985
PMID: 2985470
Yanisch-Perron C, Vieira J, Messing J., Gene 33(1), 1985
PMID: 2985470
Clustal W and Clustal X version 2.0.
Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG., Bioinformatics 23(21), 2007
PMID: 17846036
Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG., Bioinformatics 23(21), 2007
PMID: 17846036
Export
Markieren/ Markierung löschen
Markierte Publikationen
Web of Science
Dieser Datensatz im Web of Science®Quellen
PMID: 22943411
PubMed | Europe PMC
Suchen in
Google Scholar