RAMTaB: Robust Alignment of Multi-Tag Bioimages

Raza S-E-A, Epstein D, Nattkemper TW, Rajpoot N (2012)
PLoS ONE 7(2): e30894.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
In recent years, new microscopic imaging techniques have evolved to allow us to visualize several different proteins (or other biomolecules) in a visual field. Analysis of protein co-localization becomes viable because molecules can interact only when they are located close to each other. We present a novel approach to align images in a multi-tag fluorescence image stack. The proposed approach is applicable to multi-tag bioimaging systems which (a) acquire fluorescence images by sequential staining and (b) simultaneously capture a phase contrast image corresponding to each of the fluorescence images. To the best of our knowledge, there is no existing method in the literature, which addresses simultaneous registration of multi-tag bioimages and selection of the reference image in order to maximize the overall overlap between the images.
Stichworte
bioimage informatics; image registration; MELC; toponomics; TIS
Erscheinungsjahr
2012
Zeitschriftentitel
PLoS ONE
Band
7
Ausgabe
2
Seite(n)
e30894
ISSN
1932-6203
eISSN
1932-6203
Page URI
https://pub.uni-bielefeld.de/record/2455753

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Raza S-E-A, Epstein D, Nattkemper TW, Rajpoot N. RAMTaB: Robust Alignment of Multi-Tag Bioimages. PLoS ONE. 2012;7(2):e30894.
Raza, S. - E. - A., Epstein, D., Nattkemper, T. W., & Rajpoot, N. (2012). RAMTaB: Robust Alignment of Multi-Tag Bioimages. PLoS ONE, 7(2), e30894. doi:10.1371/journal.pone.0030894
Raza, S. - E. - A., Epstein, D., Nattkemper, T. W., and Rajpoot, N. (2012). RAMTaB: Robust Alignment of Multi-Tag Bioimages. PLoS ONE 7, e30894.
Raza, S.-E.-A., et al., 2012. RAMTaB: Robust Alignment of Multi-Tag Bioimages. PLoS ONE, 7(2), p e30894.
S.-E.-A. Raza, et al., “RAMTaB: Robust Alignment of Multi-Tag Bioimages”, PLoS ONE, vol. 7, 2012, pp. e30894.
Raza, S.-E.-A., Epstein, D., Nattkemper, T.W., Rajpoot, N.: RAMTaB: Robust Alignment of Multi-Tag Bioimages. PLoS ONE. 7, e30894 (2012).
Raza, Shan-E-Ahmed, Epstein, David, Nattkemper, Tim Wilhelm, and Rajpoot, Nasir. “RAMTaB: Robust Alignment of Multi-Tag Bioimages”. PLoS ONE 7.2 (2012): e30894.
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