Reconstitution of the tonoplast amino acid carrier into liposomes. Evidence for an ATP regulated carrier in different species

THUME M, Dietz K-J (1991)
Planta 185(4): 569-575.

Zeitschriftenaufsatz | Veröffentlicht| Englisch
 
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Abstract / Bemerkung
The tonoplast amino-acid transporter of barley (Hordeum vulgare L.) mesophyll cells was functionally reconstituted by incorporating solubilized tonoplast membranes, vacuoplast membranes or tonoplast-enriched microsomal vesicles into phosphatidylcholine liposomes. (i) Time-, concentration- and ATP-dependence of amino-acid uptake were similar to results with isolated vacuoles. Although the orientation of incorporation could not be controlled, the results indicate that the transporter functions as a uniport system which allows regulated equilibration by diffusion between the cytosolic and vacuolar amino-acid pools. (ii) The ATP-modulated amino-acid carrier was also successfully reconstituted from barley epidermal protoplasts and Valerianella or Tulipa vacuoplasts, indicating its general occurrence. (iii) Fractionation of solubilized tonoplasts by size-exclusion chromatography followed by reconstitution of the fractions for glutamine transport gave two activity peaks: the first eluted in the region of high-molecular-mass vesicles and the second at a size of 300 kDa for the Triton-protein micelle.
Stichworte
AMINO ACID (TRANSPORT); HORDEUM (TONOPLAST AMINO-ACID CARRIER); TONOPLAST (AMINO-ACID CARRIER); VACUOLE (AMINO ACIDS)
Erscheinungsjahr
1991
Zeitschriftentitel
Planta
Band
185
Ausgabe
4
Seite(n)
569-575
ISSN
0032-0935
Page URI
https://pub.uni-bielefeld.de/record/2408134

Zitieren

THUME M, Dietz K-J. Reconstitution of the tonoplast amino acid carrier into liposomes. Evidence for an ATP regulated carrier in different species. Planta. 1991;185(4):569-575.
THUME, M., & Dietz, K. - J. (1991). Reconstitution of the tonoplast amino acid carrier into liposomes. Evidence for an ATP regulated carrier in different species. Planta, 185(4), 569-575.
THUME, M., and Dietz, K. - J. (1991). Reconstitution of the tonoplast amino acid carrier into liposomes. Evidence for an ATP regulated carrier in different species. Planta 185, 569-575.
THUME, M., & Dietz, K.-J., 1991. Reconstitution of the tonoplast amino acid carrier into liposomes. Evidence for an ATP regulated carrier in different species. Planta, 185(4), p 569-575.
M. THUME and K.-J. Dietz, “Reconstitution of the tonoplast amino acid carrier into liposomes. Evidence for an ATP regulated carrier in different species”, Planta, vol. 185, 1991, pp. 569-575.
THUME, M., Dietz, K.-J.: Reconstitution of the tonoplast amino acid carrier into liposomes. Evidence for an ATP regulated carrier in different species. Planta. 185, 569-575 (1991).
THUME, M, and Dietz, Karl-Josef. “Reconstitution of the tonoplast amino acid carrier into liposomes. Evidence for an ATP regulated carrier in different species”. Planta 185.4 (1991): 569-575.

4 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Transport processes of solutes across the vacuolar membrane of higher plants.
Martinoia E, Massonneau A, Frangne N., Plant Cell Physiol 41(11), 2000
PMID: 11092901
Interaction of phospholipids with proteins and peptides. New advances IV.
Cserhåti T, Szögyi M., Int J Biochem 26(1), 1994
PMID: 8138037

13 References

Daten bereitgestellt von Europe PubMed Central.

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PMID: 2004114
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Kasahara M, Hinkle PC., J. Biol. Chem. 252(20), 1977
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