Immunological characterization of two dominant tonoplast polypeptides

Betz M, Dietz K-J (1991)
PLANT PHYSIOLOGY 97(4): 1294-1301.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
Abstract / Bemerkung
At least 14 distinct polypeptides reside in the tonoplast of barley (Hordeum vulgare) mesophyll vacuoles. Two of the polypeptides were isolated from two-dimensional separations of vacuoplast membrane proteins and used for immunization. With the antisera, the localization on the membrane and the distribution of the polypeptides in the plant kingdom and in various tissues of barley plants was studied. The polypeptides have an apparent molecular mass of 31 and 40 kilodaltons. After freeze-thaw cycles or washing of the membranes with 4.5 millimolar NaCl, the polypeptides were still sedimented with the membranes, suggesting an intrinsic localization. The antiserum against the 31-kilodalton polypeptide bound to the outer surface of isolated intact vacuoles. In chromatographic separations of Triton X-100-solubilized membrane fractions, the residual activities of various acid hydrolases eluted distinct from the 31- and 40-kilodalton polypeptides. Both polypeptides tend to form larger aggregates, however smaller than the tonoplast ATPase. Cross-reactive polypeptides were present in higher and lower plants (the green alga Chara corallina and the liverwort Conocephalum) and in liver tissue from rat and beef, but were not detected in other animal tissues tested so far. The results indicate a wide distribution of these tonoplast polypeptides in vacuole-containing organisms.
Page URI


Betz M, Dietz K-J. Immunological characterization of two dominant tonoplast polypeptides. PLANT PHYSIOLOGY. 1991;97(4):1294-1301.
Betz, M., & Dietz, K. - J. (1991). Immunological characterization of two dominant tonoplast polypeptides. PLANT PHYSIOLOGY, 97(4), 1294-1301.
Betz, M., and Dietz, K. - J. (1991). Immunological characterization of two dominant tonoplast polypeptides. PLANT PHYSIOLOGY 97, 1294-1301.
Betz, M., & Dietz, K.-J., 1991. Immunological characterization of two dominant tonoplast polypeptides. PLANT PHYSIOLOGY, 97(4), p 1294-1301.
M. Betz and K.-J. Dietz, “Immunological characterization of two dominant tonoplast polypeptides”, PLANT PHYSIOLOGY, vol. 97, 1991, pp. 1294-1301.
Betz, M., Dietz, K.-J.: Immunological characterization of two dominant tonoplast polypeptides. PLANT PHYSIOLOGY. 97, 1294-1301 (1991).
Betz, M., and Dietz, Karl-Josef. “Immunological characterization of two dominant tonoplast polypeptides”. PLANT PHYSIOLOGY 97.4 (1991): 1294-1301.

17 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Unraveling Hidden Components of the Chloroplast Envelope Proteome: Opportunities and Limits of Better MS Sensitivity.
Bouchnak I, Brugière S, Moyet L, Le Gall S, Salvi D, Kuntz M, Tardif M, Rolland N., Mol Cell Proteomics 18(7), 2019
PMID: 30962257
Vacuolar H+-ATPase activity is required for endocytic and secretory trafficking in Arabidopsis.
Dettmer J, Hong-Hermesdorf A, Stierhof YD, Schumacher K., Plant Cell 18(3), 2006
PMID: 16461582
Essential role of the V-ATPase in male gametophyte development.
Dettmer J, Schubert D, Calvo-Weimar O, Stierhof YD, Schmidt R, Schumacher K., Plant J 41(1), 2005
PMID: 15610354
Arabidopsis vacuolar H-ATPase subunit E isoform 1 is required for Golgi organization and vacuole function in embryogenesis.
Strompen G, Dettmer J, Stierhof YD, Schumacher K, Jürgens G, Mayer U., Plant J 41(1), 2005
PMID: 15610355
Subcellular distribution of the V-ATPase complex in plant cells, and in vivo localisation of the 100 kDa subunit VHA-a within the complex.
Kluge C, Seidel T, Bolte S, Sharma SS, Hanitzsch M, Satiat-Jeunemaitre B, Ross J, Sauer M, Golldack D, Dietz KJ., BMC Cell Biol 5(), 2004
PMID: 15310389
Alterations in Cd-induced gene expression under nitrogen deficiency in Hordeum vulgare.
Finkemeier I, Kluge C, Metwally A, Georgi M, Grotjohann N, Dietz KJ., Plant Cell Environ 26(6), 2003
PMID: 12803610
New insight into the structure and regulation of the plant vacuolar H+-ATPase.
Kluge C, Lahr J, Hanitzsch M, Bolte S, Golldack D, Dietz KJ., J Bioenerg Biomembr 35(4), 2003
PMID: 14635783
Transport of 1-kestose across the tonoplast of Jerusalem artichoke tubers.
Pontis HG, González P, Etxeberria E., Phytochemistry 59(3), 2002
PMID: 11830131


Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®


PMID: 16668546
PubMed | Europe PMC

Suchen in

Google Scholar