Characterization of the epidermis from barley primary leaves. 1. Isolation of epidermal protoplasts

Dietz K-J, SCHRAMM M, BETZ M, BUSCH H, DURR C, MARTINOIA E (1992)
Planta 187(4): 425-430.

Zeitschriftenaufsatz | Veröffentlicht| Englisch
 
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Autor/in
Dietz, Karl-JosefUniBi; SCHRAMM, M; BETZ, M; BUSCH, H; DURR, C; MARTINOIA, E
Abstract / Bemerkung
A method is described for isolating epidermal protoplasts from the primary leaves of barley (Hordeum vulgare L.). Epidermal protoplasts are lighter than mesophyll protoplasts because of their smaller ratio of cytoplasm to vacuole, and can be separated from the latter by density-gradient centrifugation after complete digestion of the leaves. We have started a basic characterization of the epidermal protoplast fraction in comparison with mesophyll protoplasts. Epidermal protoplasts had a mean diameter of 63.5-mu-m, whereas that of mesophyll protoplasts was 35.7-mu-m. Their respiratory oxygen consumption was not influenced by light. They contained acid hydrolases and cytoplasmic enzymes in relative activities different from those of mesophyll protoplasts. Their polypeptide pattern as judged from two-dimensional separations was, in principle, similar to that of mesophyll cells after elimination of the plastids from the latter by the preparation of vacuoplasts. However, in addition, a considerable number of epidermis-specific polypeptides were observed. Isolated epidermal protoplasts were viable and efficiently incorporated [S-35]methionine into newly synthesized proteins. The results show that epidermal protoplasts are suitable for the investigation of the physiological and molecular properties of epidermal cells in leaves.
Stichworte
PROTOPLAST ISOLATION; EPIDERMIS; ENZYME ACTIVITY (EPIDERMIS); HORDEUM (EPIDERMIS); POLYPEPTIDE; (EPIDERMIS); PROTEIN SYNTHESIS (EPIDERMIS)
Erscheinungsjahr
1992
Zeitschriftentitel
Planta
Band
187
Ausgabe
4
Seite(n)
425-430
ISSN
0032-0935
eISSN
1432-2048
Page URI
https://pub.uni-bielefeld.de/record/2408082

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Dietz K-J, SCHRAMM M, BETZ M, BUSCH H, DURR C, MARTINOIA E. Characterization of the epidermis from barley primary leaves. 1. Isolation of epidermal protoplasts. Planta. 1992;187(4):425-430.
Dietz, K. - J., SCHRAMM, M., BETZ, M., BUSCH, H., DURR, C., & MARTINOIA, E. (1992). Characterization of the epidermis from barley primary leaves. 1. Isolation of epidermal protoplasts. Planta, 187(4), 425-430. doi:10.1007/BF00199959
Dietz, K. - J., SCHRAMM, M., BETZ, M., BUSCH, H., DURR, C., and MARTINOIA, E. (1992). Characterization of the epidermis from barley primary leaves. 1. Isolation of epidermal protoplasts. Planta 187, 425-430.
Dietz, K.-J., et al., 1992. Characterization of the epidermis from barley primary leaves. 1. Isolation of epidermal protoplasts. Planta, 187(4), p 425-430.
K.-J. Dietz, et al., “Characterization of the epidermis from barley primary leaves. 1. Isolation of epidermal protoplasts”, Planta, vol. 187, 1992, pp. 425-430.
Dietz, K.-J., SCHRAMM, M., BETZ, M., BUSCH, H., DURR, C., MARTINOIA, E.: Characterization of the epidermis from barley primary leaves. 1. Isolation of epidermal protoplasts. Planta. 187, 425-430 (1992).
Dietz, Karl-Josef, SCHRAMM, M, BETZ, M, BUSCH, H, DURR, C, and MARTINOIA, E. “Characterization of the epidermis from barley primary leaves. 1. Isolation of epidermal protoplasts”. Planta 187.4 (1992): 425-430.

10 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

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20 References

Daten bereitgestellt von Europe PubMed Central.

Hydrolytic enzymes in the central vacuole of plant cells.
Boller T, Kende H., Plant Physiol. 63(6), 1979
PMID: 16660869

Delieu, New Phytol. 71(), 1974
Characterization of the epidermis from barley primary leaves : II. The role of the epidermis in ion compartmentation.
Dietz KJ, Schramm M, Lang B, Lanzl-Schramm A, Durr C, Martinoia E., Planta 187(4), 1992
PMID: 24178136
Circadian Stomatal Rhythms in Epidermal Peels from Vicia faba.
Gorton HL, Williams WE, Binns ME, Gemmell CN, Leheny EA, Shepherd AC., Plant Physiol. 90(4), 1989
PMID: 16666931
Profile of Basic Carbon Pathways in Guard Cells and Other Leaf Cells of Vicia faba L.
Hampp R, Outlaw WH, Tarczynski MC., Plant Physiol. 70(6), 1982
PMID: 16662723

Kaiser, Z. Pflanzenphysiol. 107(), 1982

Knuth, Plant Cell Physiol. 24(), 1983

Kolattukudy, 1977

Kolattukudy, 1980
Phosphate transport across biomembranes and cytosolic phosphate homeostasis in barley leaves.
Mimura T, Dietz KJ, Kaiser W, Schramm MJ, Kaiser G, Heber U., Planta 180(2), 1990
PMID: 24201937

Pfanz, J. Plant Physiol. 129(), 1987
Photosynthetic carbon reduction pathway is absent in chloroplasts of Vicia faba guard cells.
Outlaw WH, Manchester J, Dicamelli CA, Randall DD, Rapp B, Veith GM., Proc. Natl. Acad. Sci. U.S.A. 76(12), 1979
PMID: 16592740

Rother, Bot. Acta 101(), 1988

Steveninck, Physiol. Plant. 56(), 1982

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