Methods in mammalian cell line engineering: from random mutagenesis to sequence specific approaches

Krämer O, Klausing S, Noll T (2010)
Applied Microbiology and Biotechnology 88(2): 425-436.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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DOI
Autor*in
Krämer, OliverUniBi; Klausing, SandraUniBi; Noll, ThomasUniBi
Einrichtung
Technische Fakultät > AG Zellkulturtechnik
Centrum für Biotechnologie > Arbeitsgruppe T. Noll
Centrum für Biotechnologie > Institut für Biochemie und Biotechnik
Abstract / Bemerkung
Due to the increasing demand for recombinant proteins, the interest in mammalian cell culture, especially of Chinese hamster ovary cells, grows rapidly. This is accompanied by the desire to improve cell lines in order to achieve higher titers and a better product quality. Until recently, most cell line development procedures were based on random integration and gene amplification, but several methods for targeted genetic modification of cells have been developed. Some of those are homologous recombination, RNA interference and zinc-finger nucleases. Especially the latter two have evolved considerably and will soon become a standard for cell line engineering in research and industrial application. This review presents an overview of established as well as new and promising techniques for targeted genetic modification of mammalian cells.
Erscheinungsjahr
2010
Zeitschriftentitel
Applied Microbiology and Biotechnology
Band
88
Ausgabe
2
Seite(n)
425-436
ISSN
0175-7598
eISSN
1432-0614
Page URI
https://pub.uni-bielefeld.de/record/2396489

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Krämer O, Klausing S, Noll T. Methods in mammalian cell line engineering: from random mutagenesis to sequence specific approaches. Applied Microbiology and Biotechnology. 2010;88(2):425-436.
Krämer, O., Klausing, S., & Noll, T. (2010). Methods in mammalian cell line engineering: from random mutagenesis to sequence specific approaches. Applied Microbiology and Biotechnology, 88(2), 425-436. https://doi.org/10.1007/s00253-010-2798-6
Krämer, Oliver, Klausing, Sandra, and Noll, Thomas. 2010. “Methods in mammalian cell line engineering: from random mutagenesis to sequence specific approaches”. Applied Microbiology and Biotechnology 88 (2): 425-436.
Krämer, O., Klausing, S., and Noll, T. (2010). Methods in mammalian cell line engineering: from random mutagenesis to sequence specific approaches. Applied Microbiology and Biotechnology 88, 425-436.
Krämer, O., Klausing, S., & Noll, T., 2010. Methods in mammalian cell line engineering: from random mutagenesis to sequence specific approaches. Applied Microbiology and Biotechnology, 88(2), p 425-436.
O. Krämer, S. Klausing, and T. Noll, “Methods in mammalian cell line engineering: from random mutagenesis to sequence specific approaches”, Applied Microbiology and Biotechnology, vol. 88, 2010, pp. 425-436.
Krämer, O., Klausing, S., Noll, T.: Methods in mammalian cell line engineering: from random mutagenesis to sequence specific approaches. Applied Microbiology and Biotechnology. 88, 425-436 (2010).
Krämer, Oliver, Klausing, Sandra, and Noll, Thomas. “Methods in mammalian cell line engineering: from random mutagenesis to sequence specific approaches”. Applied Microbiology and Biotechnology 88.2 (2010): 425-436.

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PMID: 20014139
Stable expression of human alpha-2,6-sialyltransferase in Chinese hamster ovary cells: functional consequences for human erythropoietin expression and bioactivity.
Zhang X, Lok SH, Kon OL., Biochim. Biophys. Acta 1425(3), 1998
PMID: 9838208
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