Analysis of Subcellular Surface Structure, Function and Dynamics

Anselmetti D, Hansmeier N, Kalinowski J, Martini J, Merkle T, Palmisano R, Ros R, Schmied K, Sischka A, Tönsing K (2007)
Analytical and Bioanalytical Chemistry 387(1): 83-89.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
; ; ; ; ; ; ; ; ;
Abstract / Bemerkung
Analytics of single biological cells allows quantitative investigation from a structural, functional and dynamical point of view and opens novel possibilities to an unamplified subcellular analysis. In this article, we report on three different experimental methods and their applications to single cellular systems with a subcellular sensitivity down to the single molecule level. First, the subcellular surface structure of living bacteria (Corynebacterium glutamicum) was investigated with atomic force microscopy (AFM) at the resolution of individual surface layer (S-layer) proteins; discrimination of bacterial strains that lack the expression of hexagonally packed surface layer proteins was possible. Second, quantitative measurement of individual recognition events of membrane-bound receptors on living B-cells was achieved in single cell manipulation and probing experiments with optical tweezers (OT) force spectroscopy. And third, intracellular dynamics of translocating photoactivatable GFP in plant protoplasts (Nicotiana tabacum BY-2) was quantitatively monitored by two-photon laser scanning microscopy (2PLSM).
Erscheinungsjahr
Zeitschriftentitel
Analytical and Bioanalytical Chemistry
Band
387
Ausgabe
1
Seite(n)
83-89
ISSN
eISSN
PUB-ID

Zitieren

Anselmetti D, Hansmeier N, Kalinowski J, et al. Analysis of Subcellular Surface Structure, Function and Dynamics. Analytical and Bioanalytical Chemistry. 2007;387(1):83-89.
Anselmetti, D., Hansmeier, N., Kalinowski, J., Martini, J., Merkle, T., Palmisano, R., Ros, R., et al. (2007). Analysis of Subcellular Surface Structure, Function and Dynamics. Analytical and Bioanalytical Chemistry, 387(1), 83-89. doi:10.1007/s00216-006-0789-3
Anselmetti, D., Hansmeier, N., Kalinowski, J., Martini, J., Merkle, T., Palmisano, R., Ros, R., Schmied, K., Sischka, A., and Tönsing, K. (2007). Analysis of Subcellular Surface Structure, Function and Dynamics. Analytical and Bioanalytical Chemistry 387, 83-89.
Anselmetti, D., et al., 2007. Analysis of Subcellular Surface Structure, Function and Dynamics. Analytical and Bioanalytical Chemistry, 387(1), p 83-89.
D. Anselmetti, et al., “Analysis of Subcellular Surface Structure, Function and Dynamics”, Analytical and Bioanalytical Chemistry, vol. 387, 2007, pp. 83-89.
Anselmetti, D., Hansmeier, N., Kalinowski, J., Martini, J., Merkle, T., Palmisano, R., Ros, R., Schmied, K., Sischka, A., Tönsing, K.: Analysis of Subcellular Surface Structure, Function and Dynamics. Analytical and Bioanalytical Chemistry. 387, 83-89 (2007).
Anselmetti, Dario, Hansmeier, Nicole, Kalinowski, Jörg, Martini, Jörg, Merkle, Thomas, Palmisano, Ralf, Ros, Robert, Schmied, K., Sischka, Andy, and Tönsing, Katja. “Analysis of Subcellular Surface Structure, Function and Dynamics”. Analytical and Bioanalytical Chemistry 387.1 (2007): 83-89.

4 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Contour and persistence length of Corynebacterium diphtheriae pili by atomic force microscopy.
Rheinlaender J, Gräbner A, Ott L, Burkovski A, Schäffer TE., Eur Biophys J 41(6), 2012
PMID: 22588485
Endothelial f-actin depolymerization enables leukocyte transmigration.
Isac L, Thoelking G, Schwab A, Oberleithner H, Riethmuller C., Anal Bioanal Chem 399(7), 2011
PMID: 20632161
Corynebacterium diphtheriae invasion-associated protein (DIP1281) is involved in cell surface organization, adhesion and internalization in epithelial cells.
Ott L, Höller M, Gerlach RG, Hensel M, Rheinlaender J, Schäffer TE, Burkovski A., BMC Microbiol 10(), 2010
PMID: 20051108

29 References

Daten bereitgestellt von Europe PubMed Central.

Atomic force microscope.
Binnig G, Quate CF, Gerber C., Phys. Rev. Lett. 56(9), 1986
PMID: 10033323

J, J Struc Biol 119(), 1997

D, Single Mol 1(), 2000
Adhesion forces between individual ligand-receptor pairs.
Florin EL, Moy VT, Gaub HE., Science 264(5157), 1994
PMID: 8153628
Single-molecule experiments in synthetic biology: an approach to the affinity ranking of DNA-binding peptides.
Eckel R, Wilking SD, Becker A, Sewald N, Ros R, Anselmetti D., Angew. Chem. Int. Ed. Engl. 44(25), 2005
PMID: 15906400
Optical trapping and manipulation of neutral particles using lasers.
Ashkin A., Proc. Natl. Acad. Sci. U.S.A. 94(10), 1997
PMID: 9144154
Ten years of tension: single-molecule DNA mechanics.
Bustamante C, Bryant Z, Smith SB., Nature 421(6921), 2003
PMID: 12540915

A, Rev Sci Instrum 74(), 2003
Combined laser tweezers and dielectric field cage for the analysis of receptor-ligand interactions on single cells.
Reichle C, Sparbier K, Muller T, Schnelle T, Walden P, Fuhr G., Electrophoresis 22(2), 2001
PMID: 11288894

W, Trends Cell Biology 9(), 1999
Single-cell gene expression profiling.
Levsky JM, Shenoy SM, Pezo RC, Singer RH., Science 297(5582), 2002
PMID: 12161654
Single-cell MALDI: a new tool for direct peptide profiling.
Li L, Garden RW, Sweedler JV., Trends Biotechnol. 18(4), 2000
PMID: 10740261

S, Laser Physics 13(), 2003
Bioanalysis in structured microfluidic systems.
Ros A, Hellmich W, Regtmeier J, Duong TT, Anselmetti D., Electrophoresis 27(13), 2006
PMID: 16817165

N, J Biotechnology 112(), 2004
The surface (S)-layer gene cspB of Corynebacterium glutamicum is transcriptionally activated by a LuxR-type regulator and located on a 6 kb genomic island absent from the type strain ATCC 13032.
Hansmeier N, Albersmeier A, Tauch A, Damberg T, Ros R, Anselmetti D, Puhler A, Kalinowski J., Microbiology (Reading, Engl.) 152(Pt 4), 2006
PMID: 16549657

J, Proc SPIE 5860(), 2005

J, Proc SPIE 6089(), 2006
A photoactivatable GFP for selective photolabeling of proteins and cells.
Patterson GH, Lippincott-Schwartz J., Science 297(5588), 2002
PMID: 12228718
Selective photolabeling of proteins using photoactivatable GFP.
Patterson GH, Lippincott-Schwartz J., Methods 32(4), 2004
PMID: 15003607
Two-photon activation and excitation properties of PA-GFP in the 720-920-nm region.
Schneider M, Barozzi S, Testa I, Faretta M, Diaspro A., Biophys. J. 89(2), 2005
PMID: 15908572
Nonlinear magic: multiphoton microscopy in the biosciences.
Zipfel WR, Williams RM, Webb WW., Nat. Biotechnol. 21(11), 2003
PMID: 14595365

E, Faraday Discuss 111(), 1999
Theoretical analysis of single-molecule force spectroscopy experiments: heterogeneity of chemical bonds.
Raible M, Evstigneev M, Bartels FW, Eckel R, Nguyen-Duong M, Merkel R, Ros R, Anselmetti D, Reimann P., Biophys. J. 90(11), 2006
PMID: 16513778
Membrane tether formation from blebbing cells.
Dai J, Sheetz MP., Biophys. J. 77(6), 1999
PMID: 10585959

Y, Proc Natl Acad Sci (USA) 100(), 2003

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 17082883
PubMed | Europe PMC

Suchen in

Google Scholar