Single Beam Optical Tweezers Setup with Backscattered Light Detection for 3D Measurements on DNA and Nanopores

Sischka A, Kleimann C, Hachmann W, Schäfer MM, Seuffert I, Tönsing K, Anselmetti D (2008)
Review of Scientific Instruments 79(6): 063702.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
We introduce a versatile and high precision three-dimensional optical tweezers setup with minimal optical interference to measure small forces and manipulate single molecules in the vicinity of a weak reflective surface. Our tweezers system integrates an inverted optical microscope with a single IR-laser beam that is spatially filtered in an appropriate way to allow force measurements in three dimensions with remarkably high precision when operated in backscattered light detection mode. The setup was tested by overstretching a lambda-DNA in x and z directions (perpendicular and along the optical axis), and by manipulating individual lambda-DNA molecules in the vicinity of a nanopore that allowed quantitative single molecule threading experiments with minimal optical interference. (C) 2008 American Institute of Physics.
Erscheinungsjahr
Zeitschriftentitel
Review of Scientific Instruments
Band
79
Ausgabe
6
Seite(n)
063702
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Sischka A, Kleimann C, Hachmann W, et al. Single Beam Optical Tweezers Setup with Backscattered Light Detection for 3D Measurements on DNA and Nanopores. Review of Scientific Instruments. 2008;79(6):063702.
Sischka, A., Kleimann, C., Hachmann, W., Schäfer, M. M., Seuffert, I., Tönsing, K., & Anselmetti, D. (2008). Single Beam Optical Tweezers Setup with Backscattered Light Detection for 3D Measurements on DNA and Nanopores. Review of Scientific Instruments, 79(6), 063702. doi:10.1063/1.293840
Sischka, A., Kleimann, C., Hachmann, W., Schäfer, M. M., Seuffert, I., Tönsing, K., and Anselmetti, D. (2008). Single Beam Optical Tweezers Setup with Backscattered Light Detection for 3D Measurements on DNA and Nanopores. Review of Scientific Instruments 79, 063702.
Sischka, A., et al., 2008. Single Beam Optical Tweezers Setup with Backscattered Light Detection for 3D Measurements on DNA and Nanopores. Review of Scientific Instruments, 79(6), p 063702.
A. Sischka, et al., “Single Beam Optical Tweezers Setup with Backscattered Light Detection for 3D Measurements on DNA and Nanopores”, Review of Scientific Instruments, vol. 79, 2008, pp. 063702.
Sischka, A., Kleimann, C., Hachmann, W., Schäfer, M.M., Seuffert, I., Tönsing, K., Anselmetti, D.: Single Beam Optical Tweezers Setup with Backscattered Light Detection for 3D Measurements on DNA and Nanopores. Review of Scientific Instruments. 79, 063702 (2008).
Sischka, Andy, Kleimann, Christoph, Hachmann, Wiebke, Schäfer, Markus M., Seuffert, Ina, Tönsing, Katja, and Anselmetti, Dario. “Single Beam Optical Tweezers Setup with Backscattered Light Detection for 3D Measurements on DNA and Nanopores”. Review of Scientific Instruments 79.6 (2008): 063702.

12 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

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