Fast filtration for metabolome sampling of suspended animal cells
Volmer M, Northoff S, Scholz S, Thüte T, Büntemeyer H, Noll T (2011)
Biotechnology Letters 33(3): 495-502.
Zeitschriftenaufsatz
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Einrichtung
Abstract / Bemerkung
A new method for sampling suspended animal cells by fast filtration is presented that allows rapid quenching of cellular metabolism and efficient separation of the cells from culture medium. Compared to sampling with a microstructure heat exchanger or centrifugation without prior quenching, the adenylate energy charge and the measured concentrations especially of metabolites with a high turnover rate or of metabolites early in metabolic pathways were substantially higher. No leakage of ATP from the cells was observed when using iso-osmotic NaCl solution in the washing step. The combination of fast filtration and cold methanol extraction is therefore suitable for intracellular metabolomic studies of suspended animal cell cultures and superior to other methods currently applied.
Stichworte
Fast filtration;
Animal cells;
Metabolome;
Microstructure heat;
Rapid sampling;
exchanger;
Quenching
Erscheinungsjahr
2011
Zeitschriftentitel
Biotechnology Letters
Band
33
Ausgabe
3
Seite(n)
495-502
ISSN
0141-5492
eISSN
1573-6776
Page URI
https://pub.uni-bielefeld.de/record/2094364
Zitieren
Volmer M, Northoff S, Scholz S, Thüte T, Büntemeyer H, Noll T. Fast filtration for metabolome sampling of suspended animal cells. Biotechnology Letters. 2011;33(3):495-502.
Volmer, M., Northoff, S., Scholz, S., Thüte, T., Büntemeyer, H., & Noll, T. (2011). Fast filtration for metabolome sampling of suspended animal cells. Biotechnology Letters, 33(3), 495-502. https://doi.org/10.1007/s10529-010-0466-7
Volmer, Martin, Northoff, Stefan, Scholz, Sebastian, Thüte, Tobias, Büntemeyer, Heino, and Noll, Thomas. 2011. “Fast filtration for metabolome sampling of suspended animal cells”. Biotechnology Letters 33 (3): 495-502.
Volmer, M., Northoff, S., Scholz, S., Thüte, T., Büntemeyer, H., and Noll, T. (2011). Fast filtration for metabolome sampling of suspended animal cells. Biotechnology Letters 33, 495-502.
Volmer, M., et al., 2011. Fast filtration for metabolome sampling of suspended animal cells. Biotechnology Letters, 33(3), p 495-502.
M. Volmer, et al., “Fast filtration for metabolome sampling of suspended animal cells”, Biotechnology Letters, vol. 33, 2011, pp. 495-502.
Volmer, M., Northoff, S., Scholz, S., Thüte, T., Büntemeyer, H., Noll, T.: Fast filtration for metabolome sampling of suspended animal cells. Biotechnology Letters. 33, 495-502 (2011).
Volmer, Martin, Northoff, Stefan, Scholz, Sebastian, Thüte, Tobias, Büntemeyer, Heino, and Noll, Thomas. “Fast filtration for metabolome sampling of suspended animal cells”. Biotechnology Letters 33.3 (2011): 495-502.
12 Zitationen in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
Fast Filtration of Bacterial or Mammalian Suspension Cell Cultures for Optimal Metabolomics Results.
Bordag N, Janakiraman V, Nachtigall J, González Maldonado S, Bethan B, Laine JP, Fux E., PLoS One 11(7), 2016
PMID: 27438065
Bordag N, Janakiraman V, Nachtigall J, González Maldonado S, Bethan B, Laine JP, Fux E., PLoS One 11(7), 2016
PMID: 27438065
Changes in intracellular ATP-content of CHO cells as response to hyperosmolality.
Pfizenmaier J, Matuszczyk JC, Takors R., Biotechnol Prog 31(5), 2015
PMID: 26146937
Pfizenmaier J, Matuszczyk JC, Takors R., Biotechnol Prog 31(5), 2015
PMID: 26146937
Compartment-specific metabolomics for CHO reveals that ATP pools in mitochondria are much lower than in cytosol.
Matuszczyk JC, Teleki A, Pfizenmaier J, Takors R., Biotechnol J 10(10), 2015
PMID: 26179617
Matuszczyk JC, Teleki A, Pfizenmaier J, Takors R., Biotechnol J 10(10), 2015
PMID: 26179617
Rationales and approaches for studying metabolism in eukaryotic microalgae.
Veyel D, Erban A, Fehrle I, Kopka J, Schroda M., Metabolites 4(2), 2014
PMID: 24957022
Veyel D, Erban A, Fehrle I, Kopka J, Schroda M., Metabolites 4(2), 2014
PMID: 24957022
Reduced quenching and extraction time for mammalian cells using filtration and syringe extraction.
Hernández Bort JA, Shanmukam V, Pabst M, Windwarder M, Neumann L, Alchalabi A, Krebiehl G, Koellensperger G, Hann S, Sonntag D, Altmann F, Heel C, Borth N., J Biotechnol 182-183(), 2014
PMID: 24794799
Hernández Bort JA, Shanmukam V, Pabst M, Windwarder M, Neumann L, Alchalabi A, Krebiehl G, Koellensperger G, Hann S, Sonntag D, Altmann F, Heel C, Borth N., J Biotechnol 182-183(), 2014
PMID: 24794799
Effects of high passage cultivation on CHO cells: a global analysis.
Beckmann TF, Krämer O, Klausing S, Heinrich C, Thüte T, Büntemeyer H, Hoffrogge R, Noll T., Appl Microbiol Biotechnol 94(3), 2012
PMID: 22331235
Beckmann TF, Krämer O, Klausing S, Heinrich C, Thüte T, Büntemeyer H, Hoffrogge R, Noll T., Appl Microbiol Biotechnol 94(3), 2012
PMID: 22331235
Optimizing high-throughput metabolomic biomarker screening: a study of quenching solutions to freeze intracellular metabolism in CHO cells.
Kronthaler J, Gstraunthaler G, Heel C., OMICS 16(3), 2012
PMID: 22401654
Kronthaler J, Gstraunthaler G, Heel C., OMICS 16(3), 2012
PMID: 22401654
How can measurement, monitoring, modeling and control advance cell culture in industrial biotechnology?
Carrondo MJ, Alves PM, Carinhas N, Glassey J, Hesse F, Merten OW, Micheletti M, Noll T, Oliveira R, Reichl U, Staby A, Teixeira AP, Weichert H, Mandenius CF., Biotechnol J 7(12), 2012
PMID: 22949408
Carrondo MJ, Alves PM, Carinhas N, Glassey J, Hesse F, Merten OW, Micheletti M, Noll T, Oliveira R, Reichl U, Staby A, Teixeira AP, Weichert H, Mandenius CF., Biotechnol J 7(12), 2012
PMID: 22949408
Metabolite extraction from suspension-cultured mammalian cells for global metabolite profiling.
Sellick CA, Hansen R, Stephens GM, Goodacre R, Dickson AJ., Nat Protoc 6(8), 2011
PMID: 21799492
Sellick CA, Hansen R, Stephens GM, Goodacre R, Dickson AJ., Nat Protoc 6(8), 2011
PMID: 21799492
Eukaryotic metabolism: measuring compartment fluxes.
Wahrheit J, Nicolae A, Heinzle E., Biotechnol J 6(9), 2011
PMID: 21910257
Wahrheit J, Nicolae A, Heinzle E., Biotechnol J 6(9), 2011
PMID: 21910257
Proteomic and metabolomic characterization of CHO DP-12 cell lines with different high passage histories.
Beckmann T, Thüte T, Heinrich C, Büntemeyer H, Noll T., BMC Proc 5 Suppl 8(), 2011
PMID: 22373112
Beckmann T, Thüte T, Heinrich C, Büntemeyer H, Noll T., BMC Proc 5 Suppl 8(), 2011
PMID: 22373112
A method for metabolomic sampling of suspended animal cells using fast filtration.
Volmer M, Gettmann J, Scholz S, Büntemeyer H, Noll T., BMC Proc 5 Suppl 8(), 2011
PMID: 22373142
Volmer M, Gettmann J, Scholz S, Büntemeyer H, Noll T., BMC Proc 5 Suppl 8(), 2011
PMID: 22373142
18 References
Daten bereitgestellt von Europe PubMed Central.
What determines the intracellular ATP concentration.
Ataullakhanov FI, Vitvitsky VM., Biosci. Rep. 22(5-6), 2002
PMID: 12635847
Ataullakhanov FI, Vitvitsky VM., Biosci. Rep. 22(5-6), 2002
PMID: 12635847
Absolute quantitation of intracellular metabolite concentrations by an isotope ratio-based approach.
Bennett BD, Yuan J, Kimball EH, Rabinowitz JD., Nat Protoc 3(8), 2008
PMID: 18714298
Bennett BD, Yuan J, Kimball EH, Rabinowitz JD., Nat Protoc 3(8), 2008
PMID: 18714298
Appropriate sampling for intracellular amino acid analysis in five phylogenetically different yeasts.
Bolten CJ, Wittmann C., Biotechnol. Lett. 30(11), 2008
PMID: 18604477
Bolten CJ, Wittmann C., Biotechnol. Lett. 30(11), 2008
PMID: 18604477
H, 2010
Quantitative evaluation of intracellular metabolite extraction techniques for yeast metabolomics.
Canelas AB, ten Pierick A, Ras C, Seifar RM, van Dam JC, van Gulik WM, Heijnen JJ., Anal. Chem. 81(17), 2009
PMID: 19653633
Canelas AB, ten Pierick A, Ras C, Seifar RM, van Dam JC, van Gulik WM, Heijnen JJ., Anal. Chem. 81(17), 2009
PMID: 19653633
Quantitative profiling of nucleotides and related phosphate-containing metabolites in cultured mammalian cells by liquid chromatography tandem electrospray mass spectrometry.
Cordell RL, Hill SJ, Ortori CA, Barrett DA., J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 871(1), 2008
PMID: 18650133
Cordell RL, Hill SJ, Ortori CA, Barrett DA., J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 871(1), 2008
PMID: 18650133
A method for the determination of changes of glycolytic metabolites in yeast on a subsecond time scale using extraction at neutral pH.
de Koning W, van Dam K., Anal. Biochem. 204(1), 1992
PMID: 1514678
de Koning W, van Dam K., Anal. Biochem. 204(1), 1992
PMID: 1514678
Towards quantitative metabolomics of mammalian cells: development of a metabolite extraction protocol.
Dietmair S, Timmins NE, Gray PP, Nielsen LK, Kromer JO., Anal. Biochem. 404(2), 2010
PMID: 20435011
Dietmair S, Timmins NE, Gray PP, Nielsen LK, Kromer JO., Anal. Biochem. 404(2), 2010
PMID: 20435011
Comparison of quenching and extraction methodologies for metabolome analysis of Lactobacillus plantarum.
Faijes M, Mars AE, Smid EJ., Microb. Cell Fact. 6(), 2007
PMID: 17708760
Faijes M, Mars AE, Smid EJ., Microb. Cell Fact. 6(), 2007
PMID: 17708760
Simultaneous extraction of several metabolites of energy metabolism and related substances in mammalian cells: optimization using experimental design.
Ritter JB, Genzel Y, Reichl U., Anal. Biochem. 373(2), 2007
PMID: 18036549
Ritter JB, Genzel Y, Reichl U., Anal. Biochem. 373(2), 2007
PMID: 18036549
AUTHOR UNKNOWN, 0
Integrated sampling procedure for metabolome analysis.
Schaub J, Schiesling C, Reuss M, Dauner M., Biotechnol. Prog. 22(5), 2006
PMID: 17022684
Schaub J, Schiesling C, Reuss M, Dauner M., Biotechnol. Prog. 22(5), 2006
PMID: 17022684
Effective quenching processes for physiologically valid metabolite profiling of suspension cultured Mammalian cells.
Sellick CA, Hansen R, Maqsood AR, Dunn WB, Stephens GM, Goodacre R, Dickson AJ., Anal. Chem. 81(1), 2009
PMID: 19061395
Sellick CA, Hansen R, Maqsood AR, Dunn WB, Stephens GM, Goodacre R, Dickson AJ., Anal. Chem. 81(1), 2009
PMID: 19061395
Lactate efflux from sarcolemmal vesicles isolated from rainbow trout Oncorhynchus mykiss white muscle is via simple diffusion.
Sharpe RL, Milligan CL., J. Exp. Biol. 206(Pt 3), 2003
PMID: 12502775
Sharpe RL, Milligan CL., J. Exp. Biol. 206(Pt 3), 2003
PMID: 12502775
Q, Metabolomics 5(), 2009
Global metabolite analysis of yeast: evaluation of sample preparation methods.
Villas-Boas SG, Hojer-Pedersen J, Akesson M, Smedsgaard J, Nielsen J., Yeast 22(14), 2005
PMID: 16240456
Villas-Boas SG, Hojer-Pedersen J, Akesson M, Smedsgaard J, Nielsen J., Yeast 22(14), 2005
PMID: 16240456
Metabolic quenching of Corynebacterium glutamicum: efficiency of methods and impact of cold shock.
Wellerdiek M, Winterhoff D, Reule W, Brandner J, Oldiges M., Bioprocess Biosyst Eng 32(5), 2008
PMID: 19050933
Wellerdiek M, Winterhoff D, Reule W, Brandner J, Oldiges M., Bioprocess Biosyst Eng 32(5), 2008
PMID: 19050933
C, Chem Eng Technol 30(), 2007
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