Control of adhA and sucR expression by the SucR regulator in Corynebacterium glutamicum

Auchter M, Laslo T, Fleischer C, Schiller L, Arndt A, Gaigalat L, Kalinowski J, Eikmanns BJ (2011)
Journal of Biotechnology 152(3): 77-86.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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DOI
Autor*in
Auchter, Marc; Laslo, Tanja; Fleischer, Constanze; Schiller, Laura; Arndt, Annette; Gaigalat, Lars; Kalinowski, JörnUniBi; Eikmanns, Bernhard J.
Einrichtung
Centrum für Biotechnologie > Arbeitsgruppe J. Kalinowski
Centrum für Biotechnologie > Institut für Genomforschung und Systembiologie
Abstract / Bemerkung
The alcohol dehydrogenase gene adhA in Corynebacterium glutamicum is subject to a complex carbon source-dependent regulation mediated by RamA, RamB and GlxR. In this study we identified SucR as the fourth transcriptional regulator involved in expression control of the adhA gene. SucR specifically binds to the adhA promoter and acts as transcriptional repressor independent of the carbon source used. Furthermore, we found that SucR negatively controls the expression of its own gene. This negative autoregulation is mediated by binding of SucR to at least one of four identified binding sites located in the promoter region of sucR. EMSA experiments and subsequent sequence analysis led to the identification of the SucR consensus binding sequence YYAACAWMAW. This binding motif is different from the binding site (ACTCTAGGGG) recently described for SucR in the promoter region of the sucCD operon. However, we were not able to detect a specific interaction of purified SucR protein with this motif present in the sucCD promoter region.
Stichworte
Corynebacterium glutamicum; Transcriptional regulation; Ethanol metabolism; Alcohol dehydrogenase; SucR transcriptional regulator
Erscheinungsjahr
2011
Zeitschriftentitel
Journal of Biotechnology
Band
152
Ausgabe
3
Seite(n)
77-86
ISSN
0168-1656
Page URI
https://pub.uni-bielefeld.de/record/2093546

Zitieren

Auchter M, Laslo T, Fleischer C, et al. Control of adhA and sucR expression by the SucR regulator in Corynebacterium glutamicum. Journal of Biotechnology. 2011;152(3):77-86.
Auchter, M., Laslo, T., Fleischer, C., Schiller, L., Arndt, A., Gaigalat, L., Kalinowski, J., et al. (2011). Control of adhA and sucR expression by the SucR regulator in Corynebacterium glutamicum. Journal of Biotechnology, 152(3), 77-86. https://doi.org/10.1016/j.jbiotec.2011.02.003
Auchter, Marc, Laslo, Tanja, Fleischer, Constanze, Schiller, Laura, Arndt, Annette, Gaigalat, Lars, Kalinowski, Jörn, and Eikmanns, Bernhard J. 2011. “Control of adhA and sucR expression by the SucR regulator in Corynebacterium glutamicum”. Journal of Biotechnology 152 (3): 77-86.
Auchter, M., Laslo, T., Fleischer, C., Schiller, L., Arndt, A., Gaigalat, L., Kalinowski, J., and Eikmanns, B. J. (2011). Control of adhA and sucR expression by the SucR regulator in Corynebacterium glutamicum. Journal of Biotechnology 152, 77-86.
Auchter, M., et al., 2011. Control of adhA and sucR expression by the SucR regulator in Corynebacterium glutamicum. Journal of Biotechnology, 152(3), p 77-86.
M. Auchter, et al., “Control of adhA and sucR expression by the SucR regulator in Corynebacterium glutamicum”, Journal of Biotechnology, vol. 152, 2011, pp. 77-86.
Auchter, M., Laslo, T., Fleischer, C., Schiller, L., Arndt, A., Gaigalat, L., Kalinowski, J., Eikmanns, B.J.: Control of adhA and sucR expression by the SucR regulator in Corynebacterium glutamicum. Journal of Biotechnology. 152, 77-86 (2011).
Auchter, Marc, Laslo, Tanja, Fleischer, Constanze, Schiller, Laura, Arndt, Annette, Gaigalat, Lars, Kalinowski, Jörn, and Eikmanns, Bernhard J. “Control of adhA and sucR expression by the SucR regulator in Corynebacterium glutamicum”. Journal of Biotechnology 152.3 (2011): 77-86.

5 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

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