The Rhizobium leguminosarum bv. viciae glnD gene, encoding a uridylyltransferase/uridylyl-removing enzyme, is expressed in the root nodule but is not essential for nitrogen fixation

Schlüter A, Nohlen M, Kramer M, Defez R, Priefer UB (2000)
Microbiology 146(11): 2987-2996.

Zeitschriftenaufsatz | Veröffentlicht| Englisch
 
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Autor/in
Schlüter, AndreasUniBi ; Nohlen, M.; Kramer, M.; Defez, R.; Priefer, U. B.
Abstract / Bemerkung
A Rhizobium leguminosarum bv. viciae VF39 gene (glnD) encoding the uridylyltransferase/uridylyl-removing enzyme, which constitutes the sensory component of the nitrogen regulation (ntr) system, was identified, cloned and characterized. The deduced amino acid sequence contains the conserved active site motif of the nucleotidyltransferase superfamily and is highly homologous to the glnD gene products of other bacterial species. Downstream of the VF39 glnD resides an open reading frame with similarity to the Salmonella typhimurium virulence factor gene mviN. Mutation of the glnD gene abolished the ability to use nitrate as a sole nitrogen source but not glutamine. In addition, neither uridylylation of P(II) nor induction of the ntr-regulated glnII gene (encoding glutamine synthetase II) under ammonium deficiency could be observed in mutant strains. This strongly suggests that glnD mutants harbour a permanently deuridylylated P(II) protein and as a consequence are unable to activate transcription from NtrC-dependent promoters. The glnD gene itself is expressed constitutively, irrespective of the nitrogen content of the medium. A functional GlnD protein is not essential for nitrogen fixation in R. leguminosarum bv. viciae, but in situ detection of glnD expression in the symbiotic and infection zone of the root nodule and quantitative measurements suggest that at least part of the ntr system functions in symbiosis. The results also indicate that the N-terminal part of GlnD is essential for the cell, as deletions in the 5'-region of the gene appear to be lethal and mutations possibly affecting the expression of the first half of the protein have a significant effect on the vitality of the mutant strain.
Erscheinungsjahr
2000
Zeitschriftentitel
Microbiology
Band
146
Ausgabe
11
Seite(n)
2987-2996
ISSN
1350-0872
Page URI
https://pub.uni-bielefeld.de/record/2092286

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Schlüter A, Nohlen M, Kramer M, Defez R, Priefer UB. The Rhizobium leguminosarum bv. viciae glnD gene, encoding a uridylyltransferase/uridylyl-removing enzyme, is expressed in the root nodule but is not essential for nitrogen fixation. Microbiology. 2000;146(11):2987-2996.
Schlüter, A., Nohlen, M., Kramer, M., Defez, R., & Priefer, U. B. (2000). The Rhizobium leguminosarum bv. viciae glnD gene, encoding a uridylyltransferase/uridylyl-removing enzyme, is expressed in the root nodule but is not essential for nitrogen fixation. Microbiology, 146(11), 2987-2996. doi:10.1099/00221287-146-11-2987
Schlüter, A., Nohlen, M., Kramer, M., Defez, R., and Priefer, U. B. (2000). The Rhizobium leguminosarum bv. viciae glnD gene, encoding a uridylyltransferase/uridylyl-removing enzyme, is expressed in the root nodule but is not essential for nitrogen fixation. Microbiology 146, 2987-2996.
Schlüter, A., et al., 2000. The Rhizobium leguminosarum bv. viciae glnD gene, encoding a uridylyltransferase/uridylyl-removing enzyme, is expressed in the root nodule but is not essential for nitrogen fixation. Microbiology, 146(11), p 2987-2996.
A. Schlüter, et al., “The Rhizobium leguminosarum bv. viciae glnD gene, encoding a uridylyltransferase/uridylyl-removing enzyme, is expressed in the root nodule but is not essential for nitrogen fixation”, Microbiology, vol. 146, 2000, pp. 2987-2996.
Schlüter, A., Nohlen, M., Kramer, M., Defez, R., Priefer, U.B.: The Rhizobium leguminosarum bv. viciae glnD gene, encoding a uridylyltransferase/uridylyl-removing enzyme, is expressed in the root nodule but is not essential for nitrogen fixation. Microbiology. 146, 2987-2996 (2000).
Schlüter, Andreas, Nohlen, M., Kramer, M., Defez, R., and Priefer, U. B. “The Rhizobium leguminosarum bv. viciae glnD gene, encoding a uridylyltransferase/uridylyl-removing enzyme, is expressed in the root nodule but is not essential for nitrogen fixation”. Microbiology 146.11 (2000): 2987-2996.

11 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Legumes symbioses: absence of Nod genes in photosynthetic bradyrhizobia.
Giraud E, Moulin L, Vallenet D, Barbe V, Cytryn E, Avarre JC, Jaubert M, Simon D, Cartieaux F, Prin Y, Bena G, Hannibal L, Fardoux J, Kojadinovic M, Vuillet L, Lajus A, Cruveiller S, Rouy Z, Mangenot S, Segurens B, Dossat C, Franck WL, Chang WS, Saunders E, Bruce D, Richardson P, Normand P, Dreyfus B, Pignol D, Stacey G, Emerich D, Verméglio A, Médigue C, Sadowsky M., Science 316(5829), 2007
PMID: 17540897
Mutational analysis of GstI protein, a glutamine synthetase translational inhibitor of Rhizobium leguminosarum.
Napolitani C, Mandrich L, Riccio A, Lamberti A, Manco G, Patriarca EJ., FEBS Lett 558(1-3), 2004
PMID: 14759514
Key role of bacterial NH(4)(+) metabolism in Rhizobium-plant symbiosis.
Patriarca EJ, Tatè R, Iaccarino M., Microbiol Mol Biol Rev 66(2), 2002
PMID: 12040124
P(II) signal transduction proteins, pivotal players in microbial nitrogen control.
Arcondéguy T, Jack R, Merrick M., Microbiol Mol Biol Rev 65(1), 2001
PMID: 11238986

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