HMGB6 from Arabidopsis thaliana specifies a novel type of plant chromosomal HMGB protein

Grasser KD, Grill S, Duroux M, Launholt D, Thomsen MS, Nielsen BV, Nielsen HK, Merkle T (2004)
Biochemistry 43(5): 1309-1314.

Zeitschriftenaufsatz | Veröffentlicht| Englisch
 
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Autor/in
Grasser, Klaus D; Grill, Simon; Duroux, Meg; Launholt, Dorte; Thomsen, Malene S; Nielsen, Birthe V; Nielsen, Hanne K; Merkle, ThomasUniBi
Abstract / Bemerkung
The high-mobility group (HMG) proteins of the HMGB family are chromatin-associated proteins that act as architectural factors in various nucleoprotein structures, which regulate DNA-dependent processes such as transcription and recombination. Database analyses revealed that in addition to the previously identified HMGB1-HMGB5 proteins, the Arabidopsis genome encodes at least three other family members having the typical overall structure of a central HMG-box DNA binding domain, which is flanked by basic and acidic regions. These novel HMGB proteins display some structural differences, when compared to HMGB1-HMGB5. Therefore, a representative of the identified proteins, now termed HMGB6, was further analyzed. The HMGB6 protein of approximately 27 kDa is the largest plant HMGB protein identified so far. This is essentially due to its unusually extended N-terminal domain of 109 amino acid residues. Subcellular localization experiments demonstrate that it is a nuclear protein. According to CD measurements, HMGB6 has an alpha-helical HMG-box domain. HMGB6 can bind DNA structure-specifically, and it is a substrate for the protein kinase CK2alpha. Because of these features, HMGB6, and presumably its relatives, can be considered members of the plant HMGB protein family. Hence, eight different chromosomal HMGB proteins are expressed in Arabidopsis, and they may serve specialized architectural functions assisting various DNA-dependent processes.
Stichworte
Casein Kinase II; Cell Nucleus; Chromosomes; Sequence Homology; Amino Acid; Sequence Analysis; Protein; Sequence Alignment; Recombinant Proteins; Protein-Serine-Threonine Kinases; Structure-Activity Relationship; Phosphorylation; Nuclear Proteins; Molecular Sequence Data; HMGB Proteins; Plant; DNA-Binding Proteins; Arabidopsis Proteins; Amino Acid Sequence
Erscheinungsjahr
2004
Zeitschriftentitel
Biochemistry
Band
43
Ausgabe
5
Seite(n)
1309-1314
ISSN
0006-2960
eISSN
1520-4995
Page URI
https://pub.uni-bielefeld.de/record/1996206

Zitieren

Grasser KD, Grill S, Duroux M, et al. HMGB6 from Arabidopsis thaliana specifies a novel type of plant chromosomal HMGB protein. Biochemistry. 2004;43(5):1309-1314.
Grasser, K. D., Grill, S., Duroux, M., Launholt, D., Thomsen, M. S., Nielsen, B. V., Nielsen, H. K., et al. (2004). HMGB6 from Arabidopsis thaliana specifies a novel type of plant chromosomal HMGB protein. Biochemistry, 43(5), 1309-1314. doi:10.1021/bi035931c
Grasser, K. D., Grill, S., Duroux, M., Launholt, D., Thomsen, M. S., Nielsen, B. V., Nielsen, H. K., and Merkle, T. (2004). HMGB6 from Arabidopsis thaliana specifies a novel type of plant chromosomal HMGB protein. Biochemistry 43, 1309-1314.
Grasser, K.D., et al., 2004. HMGB6 from Arabidopsis thaliana specifies a novel type of plant chromosomal HMGB protein. Biochemistry, 43(5), p 1309-1314.
K.D. Grasser, et al., “HMGB6 from Arabidopsis thaliana specifies a novel type of plant chromosomal HMGB protein”, Biochemistry, vol. 43, 2004, pp. 1309-1314.
Grasser, K.D., Grill, S., Duroux, M., Launholt, D., Thomsen, M.S., Nielsen, B.V., Nielsen, H.K., Merkle, T.: HMGB6 from Arabidopsis thaliana specifies a novel type of plant chromosomal HMGB protein. Biochemistry. 43, 1309-1314 (2004).
Grasser, Klaus D, Grill, Simon, Duroux, Meg, Launholt, Dorte, Thomsen, Malene S, Nielsen, Birthe V, Nielsen, Hanne K, and Merkle, Thomas. “HMGB6 from Arabidopsis thaliana specifies a novel type of plant chromosomal HMGB protein”. Biochemistry 43.5 (2004): 1309-1314.

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