Quantitative proteomic overview on the Corynebacterium glutamicum L-lysine producing strain DM1730

Fraenzel B, Poetsch A, Troetschel C, Persicke M, Kalinowski J, Wolters DA (2010)
Journal of Proteomics 73(12): 2336-2353.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Corynebacterium glutamicum is one of the most important microorganisms because of its ability to produce and secrete glutamate, lysine and other amino acids. To optimize biotechnological amino acid synthesis it is therefore necessary to understand well how metabolic fluxes can be altered by studying the proteins directing these fluxes. In this work we give a comprehensive quantitative outline about the proteomic state of the L-lysine producing mutant strain DM1730 compared to wild type strain ATCC 13032 in the stationary phase of growth. This study comprises 1107 soluble and membrane proteins, of which 908 have been quantified. C. glutamicum DM1730 seems to produce a large amount of lysine even at the expense of various housekeeping functions. Generally, several proteins that are involved in stress response were found to be significantly more abundant, whereas many members of the protein expression machinery are less abundant as well as most proteins involved in cell growth and division and cell envelope synthesis. Extensive L-lysine production causes C. glutamicum to suffer from oxidative stress and iron limitation. Ultimately, a changed lipid composition of C. glutamicum's cell envelope seems to increase its fluidity, which might be related to altered physiology and membrane processes. (C) 2010 Elsevier B.V. All rights reserved.
Stichworte
Altered membrane lipid composition; Biphasic partitioning system; Oxidative stress; proteome; Corynebacterium glutamicum; L-Lysine producing strain; Membrane; Gram-positive bacteria; DM1730
Erscheinungsjahr
2010
Zeitschriftentitel
Journal of Proteomics
Band
73
Ausgabe
12
Seite(n)
2336-2353
ISSN
1874-3919
Page URI
https://pub.uni-bielefeld.de/record/1968272

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Fraenzel B, Poetsch A, Troetschel C, Persicke M, Kalinowski J, Wolters DA. Quantitative proteomic overview on the Corynebacterium glutamicum L-lysine producing strain DM1730. Journal of Proteomics. 2010;73(12):2336-2353.
Fraenzel, B., Poetsch, A., Troetschel, C., Persicke, M., Kalinowski, J., & Wolters, D. A. (2010). Quantitative proteomic overview on the Corynebacterium glutamicum L-lysine producing strain DM1730. Journal of Proteomics, 73(12), 2336-2353. doi:10.1016/j.jprot.2010.07.006
Fraenzel, B., Poetsch, A., Troetschel, C., Persicke, M., Kalinowski, J., and Wolters, D. A. (2010). Quantitative proteomic overview on the Corynebacterium glutamicum L-lysine producing strain DM1730. Journal of Proteomics 73, 2336-2353.
Fraenzel, B., et al., 2010. Quantitative proteomic overview on the Corynebacterium glutamicum L-lysine producing strain DM1730. Journal of Proteomics, 73(12), p 2336-2353.
B. Fraenzel, et al., “Quantitative proteomic overview on the Corynebacterium glutamicum L-lysine producing strain DM1730”, Journal of Proteomics, vol. 73, 2010, pp. 2336-2353.
Fraenzel, B., Poetsch, A., Troetschel, C., Persicke, M., Kalinowski, J., Wolters, D.A.: Quantitative proteomic overview on the Corynebacterium glutamicum L-lysine producing strain DM1730. Journal of Proteomics. 73, 2336-2353 (2010).
Fraenzel, Benjamin, Poetsch, Ansgar, Troetschel, Christian, Persicke, Marcus, Kalinowski, Jörn, and Wolters, Dirk Andreas. “Quantitative proteomic overview on the Corynebacterium glutamicum L-lysine producing strain DM1730”. Journal of Proteomics 73.12 (2010): 2336-2353.

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