Inhibition of furin by serpin Spn4A from Drosophila melanogaster

Oley M, Letzel M, Ragg H (2004)
FEBS Letters 577(1-2): 165-169.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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DOI
Autor*in
Oley, Mareke; Letzel, MatthiasUniBi; Ragg, HermannUniBi
Einrichtung
Fakultät für Chemie
Technische Fakultät > AG Zelluläre Genetik
Abstract / Bemerkung
The serpin gene Spn4 from Drosophila melanogaster encodes multiple isoforms with alternative reactive site loops (RSL). Here, we show that isoform Spn4A inhibits human furin with an apparent k(assoc) of 5.5 x 10(6) M-1 s(-1). The serpin forms SDS-stable complexes with the enzyme and the RSL of Spn4A is cleaved C-terminally to the sequence -Arg-Arg-Lys-Argdown arrow in accord with the recognition/cleavage site of furin. Immunofluorescence studies show that Spn4A is localized in the endoplasmic reticulum (ER), suggesting that the inhibitor is an interesting tool for investigating the cellular mechanisms regulating furin and for the design of agents controlling prohormone convertases. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Stichworte
endoplasmic reticulum; protease inhibitor; furin; serpin; Drosophila; melanogaster
Erscheinungsjahr
2004
Zeitschriftentitel
FEBS Letters
Band
577
Ausgabe
1-2
Seite(n)
165-169
ISSN
0014-5793
Page URI
https://pub.uni-bielefeld.de/record/1953787

Zitieren

Oley M, Letzel M, Ragg H. Inhibition of furin by serpin Spn4A from Drosophila melanogaster. FEBS Letters. 2004;577(1-2):165-169.
Oley, M., Letzel, M., & Ragg, H. (2004). Inhibition of furin by serpin Spn4A from Drosophila melanogaster. FEBS Letters, 577(1-2), 165-169. https://doi.org/10.1016/j.febslet.2004.10.003
Oley, Mareke, Letzel, Matthias, and Ragg, Hermann. 2004. “Inhibition of furin by serpin Spn4A from Drosophila melanogaster”. FEBS Letters 577 (1-2): 165-169.
Oley, M., Letzel, M., and Ragg, H. (2004). Inhibition of furin by serpin Spn4A from Drosophila melanogaster. FEBS Letters 577, 165-169.
Oley, M., Letzel, M., & Ragg, H., 2004. Inhibition of furin by serpin Spn4A from Drosophila melanogaster. FEBS Letters, 577(1-2), p 165-169.
M. Oley, M. Letzel, and H. Ragg, “Inhibition of furin by serpin Spn4A from Drosophila melanogaster”, FEBS Letters, vol. 577, 2004, pp. 165-169.
Oley, M., Letzel, M., Ragg, H.: Inhibition of furin by serpin Spn4A from Drosophila melanogaster. FEBS Letters. 577, 165-169 (2004).
Oley, Mareke, Letzel, Matthias, and Ragg, Hermann. “Inhibition of furin by serpin Spn4A from Drosophila melanogaster”. FEBS Letters 577.1-2 (2004): 165-169.

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