Deficiency of UDP-galactose:N-acetylglucosamine beta-1,4-galactosyltransferase I causes the congenital disorder of glycosylation type IId

Hansske B, Thiel C, Lübke T, Hasilik M, Höning S, Peters V, Heidemann PH, Hoffmann GF, Berger EG, von Figura K, Körner C (2002)
Journal of Clinical Investigation 109(6): 725-733.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Hansske, Bengt; Thiel, Christian; Lübke, TorbenUniBi ; Hasilik, Martin; Höning, Stefan; Peters, Verena; Heidemann, Peter H.; Hoffmann, Georg F.; Berger, Eric G.; von Figura, Kurt; Körner, Christian
Abstract / Bemerkung
Deficiency of the Golgi enzyme UDP-Gal:N-acetylglucosamine β-1,4-galactosyltransferase I (β4GalT I) (E.C.2.4.1.38) causes a new congenital disorder of glycosylation (CDG), designated type IId (CDG-IId), a severe neurologic disease characterized by a hydrocephalus, myopathy, and blood-clotting defects. Analysis of oligosaccharides from serum transferrin by HPLC, mass spectrometry, and lectin binding revealed the loss of sialic acid and galactose residues. In skin fibroblasts and leukocytes, galactosyltransferase activity was reduced to 5% that of controls. In fibroblasts, a truncated polypeptide was detected that was about 12 kDa smaller in size than wild-type β4GalT I and that failed to localize to the Golgi apparatus. Sequencing of the β4GalT I cDNA and gene revealed an insertion of a single nucleotide (1031-1032insC) leading to premature translation stop and loss of the C-terminal 50 amino acids of the enzyme. The patient was homozygous and his parents heterozygous for this mutation. Expression of a corresponding mutant cDNA in COS-7 cells led to the synthesis of a truncated, inactive polypeptide, which localized to the endoplasmic reticulum.
Erscheinungsjahr
2002
Zeitschriftentitel
Journal of Clinical Investigation
Band
109
Ausgabe
6
Seite(n)
725-733
Page URI
https://pub.uni-bielefeld.de/record/1940103

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Hansske B, Thiel C, Lübke T, et al. Deficiency of UDP-galactose:N-acetylglucosamine beta-1,4-galactosyltransferase I causes the congenital disorder of glycosylation type IId. Journal of Clinical Investigation. 2002;109(6):725-733.
Hansske, B., Thiel, C., Lübke, T., Hasilik, M., Höning, S., Peters, V., Heidemann, P. H., et al. (2002). Deficiency of UDP-galactose:N-acetylglucosamine beta-1,4-galactosyltransferase I causes the congenital disorder of glycosylation type IId. Journal of Clinical Investigation, 109(6), 725-733. https://doi.org/10.1172/JCI14010
Hansske, B., Thiel, C., Lübke, T., Hasilik, M., Höning, S., Peters, V., Heidemann, P. H., Hoffmann, G. F., Berger, E. G., von Figura, K., et al. (2002). Deficiency of UDP-galactose:N-acetylglucosamine beta-1,4-galactosyltransferase I causes the congenital disorder of glycosylation type IId. Journal of Clinical Investigation 109, 725-733.
Hansske, B., et al., 2002. Deficiency of UDP-galactose:N-acetylglucosamine beta-1,4-galactosyltransferase I causes the congenital disorder of glycosylation type IId. Journal of Clinical Investigation, 109(6), p 725-733.
B. Hansske, et al., “Deficiency of UDP-galactose:N-acetylglucosamine beta-1,4-galactosyltransferase I causes the congenital disorder of glycosylation type IId”, Journal of Clinical Investigation, vol. 109, 2002, pp. 725-733.
Hansske, B., Thiel, C., Lübke, T., Hasilik, M., Höning, S., Peters, V., Heidemann, P.H., Hoffmann, G.F., Berger, E.G., von Figura, K., Körner, C.: Deficiency of UDP-galactose:N-acetylglucosamine beta-1,4-galactosyltransferase I causes the congenital disorder of glycosylation type IId. Journal of Clinical Investigation. 109, 725-733 (2002).
Hansske, Bengt, Thiel, Christian, Lübke, Torben, Hasilik, Martin, Höning, Stefan, Peters, Verena, Heidemann, Peter H., Hoffmann, Georg F., Berger, Eric G., von Figura, Kurt, and Körner, Christian. “Deficiency of UDP-galactose:N-acetylglucosamine beta-1,4-galactosyltransferase I causes the congenital disorder of glycosylation type IId”. Journal of Clinical Investigation 109.6 (2002): 725-733.

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PMID: 11901181
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