RamA and RamB are global transcriptional regulators in Corynebacterium glutamicum and control genes for enzymes of the central metabolism

Auchter M, Cramer A, Hüser A, Rückert C, Emer D, Schwarz P, Arndt A, Lange C, Kalinowski J, Wendisch VF, Eikmanns BJ (2011)
J Biotechnol 154(2-3): 126-139.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Autor/in
; ; ; ; ; ; ; ; ; ;
Abstract / Bemerkung
Expression profiling of Corynebacterium glutamicum in comparison to a derivative deficient in the transcriptional regulator AtlR (previously known as SucR or MtlR) revealed eight genes showing more than 4-fold higher mRNA levels in the mutant. Four of these genes are located in the direct vicinity of the atlR gene, i.e., xylB, rbtT, mtlD, and sixA, annotated as encoding xylulokinase, the ribitol transporter, mannitol 2-dehydrogenase, and phosphohistidine phosphatase, respectively. Transcriptional analysis indicated that atlR and the four genes are organized as atlR-xylB and rbtT-mtlD-sixA operons. Growth experiments with C. glutamicum and C. glutamicum ΔatlR, ΔxylB, ΔrbtT, ΔmtlD, and ΔsixA derivatives with sugar alcohols revealed that (i) wild-type C. glutamicum grows on D-arabitol but not on other sugar alcohols, (ii) growth in the presence of D-arabitol allows subsequent growth on D-mannitol, (iii) D-arabitol is cometabolized with glucose and preferentially utilized over D-mannitol, (iv) RbtT and XylB are involved in D-arabitol but not in D-mannitol metabolism, (v) MtlD is required for D-arabitol and D-mannitol metabolism, and (vi) SixA is not required for growth on any of the substrates tested. Furthermore, we show that MtlD confers D-arabitol and D-mannitol dehydrogenase activities, that the levels of these and also xylulokinase activities are generally high in the C. glutamicum ΔatlR mutant, whereas in the parental strain, they were high when cells were grown in the presence of D-arabitol and very low when cells were grown in its absence. Our results show that the XylB, RbtT, and MtlD proteins allow the growth of C. glutamicum on D-arabitol and that D-arabitol metabolism is subject to arabitol-dependent derepression by AtlR.
Erscheinungsjahr
2011
Zeitschriftentitel
J Biotechnol
Band
154
Ausgabe
2-3
Seite(n)
126-139
ISSN
0168-1656
Page URI
https://pub.uni-bielefeld.de/record/1896635

Zitieren

Auchter M, Cramer A, Hüser A, et al. RamA and RamB are global transcriptional regulators in Corynebacterium glutamicum and control genes for enzymes of the central metabolism. J Biotechnol. 2011;154(2-3):126-139.
Auchter, M., Cramer, A., Hüser, A., Rückert, C., Emer, D., Schwarz, P., Arndt, A., et al. (2011). RamA and RamB are global transcriptional regulators in Corynebacterium glutamicum and control genes for enzymes of the central metabolism. J Biotechnol, 154(2-3), 126-139. doi:10.1016/j.jbiotec.2010.07.001
Auchter, M., Cramer, A., Hüser, A., Rückert, C., Emer, D., Schwarz, P., Arndt, A., Lange, C., Kalinowski, J., Wendisch, V. F., et al. (2011). RamA and RamB are global transcriptional regulators in Corynebacterium glutamicum and control genes for enzymes of the central metabolism. J Biotechnol 154, 126-139.
Auchter, M., et al., 2011. RamA and RamB are global transcriptional regulators in Corynebacterium glutamicum and control genes for enzymes of the central metabolism. J Biotechnol, 154(2-3), p 126-139.
M. Auchter, et al., “RamA and RamB are global transcriptional regulators in Corynebacterium glutamicum and control genes for enzymes of the central metabolism”, J Biotechnol, vol. 154, 2011, pp. 126-139.
Auchter, M., Cramer, A., Hüser, A., Rückert, C., Emer, D., Schwarz, P., Arndt, A., Lange, C., Kalinowski, J., Wendisch, V.F., Eikmanns, B.J.: RamA and RamB are global transcriptional regulators in Corynebacterium glutamicum and control genes for enzymes of the central metabolism. J Biotechnol. 154, 126-139 (2011).
Auchter, M., Cramer, A., Hüser, A., Rückert, Christian, Emer, D., Schwarz, P., Arndt, A., Lange, C., Kalinowski, Jörn, Wendisch, Volker F., and Eikmanns, B. J. “RamA and RamB are global transcriptional regulators in Corynebacterium glutamicum and control genes for enzymes of the central metabolism”. J Biotechnol 154.2-3 (2011): 126-139.

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 22178972
PubMed | Europe PMC

Suchen in

Google Scholar