Plant heterotrimeric G protein beta subunit is associated with membranes via protein interactions involving coiled-coil formation.

Obrdlik P, Neuhaus G, Merkle T (2000)
FEBS Letters 476(3): 208-212.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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DOI
Autor*in
Obrdlik, P.; Neuhaus, G.; Merkle, ThomasUniBi
Einrichtung
Fakultät für Biologie > Genetik und Genomik der Pflanzen
Abstract / Bemerkung
Gbeta subunits from animals are anchored to membranes via Ggamma subunits. No Ggamma has been identified in plants to date. Using differential centrifugation of Arabidopsis and broccoli extracts, Gbeta was highly enriched in the microsomal pellet. Treatment of microsomes with detergents and salts indicates that plant Gbeta is located at the membrane surface and attached to membranes by hydrophobic interactions. Analysis of transgenic plants expressing Gbeta-GFP fusion proteins showed that mutations in the heptad repeat domain of Gbeta severely diminished their membrane association. We propose that plant Gbeta is anchored to membranes by an unknown protein similar to animal Gbeta by Ggamma, via coiled-coil formation.
Erscheinungsjahr
2000
Zeitschriftentitel
FEBS Letters
Band
476
Ausgabe
3
Seite(n)
208-212
ISSN
0014-5793
Page URI
https://pub.uni-bielefeld.de/record/1867328

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Obrdlik P, Neuhaus G, Merkle T. Plant heterotrimeric G protein beta subunit is associated with membranes via protein interactions involving coiled-coil formation. FEBS Letters. 2000;476(3):208-212.
Obrdlik, P., Neuhaus, G., & Merkle, T. (2000). Plant heterotrimeric G protein beta subunit is associated with membranes via protein interactions involving coiled-coil formation. FEBS Letters, 476(3), 208-212. https://doi.org/10.1016/S0014-5793(00)01706-3
Obrdlik, P., Neuhaus, G., and Merkle, Thomas. 2000. “Plant heterotrimeric G protein beta subunit is associated with membranes via protein interactions involving coiled-coil formation.”. FEBS Letters 476 (3): 208-212.
Obrdlik, P., Neuhaus, G., and Merkle, T. (2000). Plant heterotrimeric G protein beta subunit is associated with membranes via protein interactions involving coiled-coil formation. FEBS Letters 476, 208-212.
Obrdlik, P., Neuhaus, G., & Merkle, T., 2000. Plant heterotrimeric G protein beta subunit is associated with membranes via protein interactions involving coiled-coil formation. FEBS Letters, 476(3), p 208-212.
P. Obrdlik, G. Neuhaus, and T. Merkle, “Plant heterotrimeric G protein beta subunit is associated with membranes via protein interactions involving coiled-coil formation.”, FEBS Letters, vol. 476, 2000, pp. 208-212.
Obrdlik, P., Neuhaus, G., Merkle, T.: Plant heterotrimeric G protein beta subunit is associated with membranes via protein interactions involving coiled-coil formation. FEBS Letters. 476, 208-212 (2000).
Obrdlik, P., Neuhaus, G., and Merkle, Thomas. “Plant heterotrimeric G protein beta subunit is associated with membranes via protein interactions involving coiled-coil formation.”. FEBS Letters 476.3 (2000): 208-212.

17 Zitationen in Europe PMC

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