Genomic and phenotypic characterization of a nonpigmented variant of Pantoea vagans biocontrol strain C9-1 lacking the 530-kb megaplasmid pPag3

Smits THM, Rezzonico F, Pelludat C, Goesmann A, Frey JE, Duffy B (2010)
FEMS MICROBIOLOGY LETTERS 308(1): 48-54.

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DOI
Autor*in
Smits, Theo H. M.; Rezzonico, Fabio; Pelludat, Cosima; Goesmann, AlexanderUniBi ; Frey, Juerg E.; Duffy, Brion
Einrichtung
Centrum für Biotechnologie > Institut für Bioinformatik
Centrum für Biotechnologie > Technologieplattformen > Bioinformatics Resource Facility
Technische Fakultät > Computational Genomics
Centrum für Biotechnologie > Arbeitsgruppe A. Goesmann
Abstract / Bemerkung
A 530-kb megaplasmid pPag3 contributing 10.8% of the total genome of Pantoea vagans biocontrol strain C9-1 was sequenced. A rare nonpigmented variant C9-1W was obtained and shown to have lost pPag3, but retained all other plasmids (pPag1, pPag2). Phenotypic characterization of the variant confirmed the function of several annotated genes that may influence ecological fitness and efficacy. Metabolic profiling revealed important plasmid-based carbon utilization phenotypes. Plasmid loss resulted in thiamine auxotrophy, absence of carotenoid pigmentation, desferrioxamine diffusible siderophore biosynthesis, inherent ampicillin resistance and expression of AI-1 quorum-sensing signaling. This confirmed the functional expression of the corresponding genes located on pPag3 in P. vagans.
Stichworte
autoinduction; biological control; carotenoids; desferrioxamine; ampicillin; Pantoea vagans
Erscheinungsjahr
2010
Zeitschriftentitel
FEMS MICROBIOLOGY LETTERS
Band
308
Ausgabe
1
Seite(n)
48-54
ISSN
0378-1097
eISSN
1574-6968
Page URI
https://pub.uni-bielefeld.de/record/1795628

Zitieren

Smits THM, Rezzonico F, Pelludat C, Goesmann A, Frey JE, Duffy B. Genomic and phenotypic characterization of a nonpigmented variant of Pantoea vagans biocontrol strain C9-1 lacking the 530-kb megaplasmid pPag3. FEMS MICROBIOLOGY LETTERS. 2010;308(1):48-54.
Smits, T. H. M., Rezzonico, F., Pelludat, C., Goesmann, A., Frey, J. E., & Duffy, B. (2010). Genomic and phenotypic characterization of a nonpigmented variant of Pantoea vagans biocontrol strain C9-1 lacking the 530-kb megaplasmid pPag3. FEMS MICROBIOLOGY LETTERS, 308(1), 48-54. https://doi.org/10.1111/j.1574-6968.2010.01994.x
Smits, Theo H. M., Rezzonico, Fabio, Pelludat, Cosima, Goesmann, Alexander, Frey, Juerg E., and Duffy, Brion. 2010. “Genomic and phenotypic characterization of a nonpigmented variant of Pantoea vagans biocontrol strain C9-1 lacking the 530-kb megaplasmid pPag3”. FEMS MICROBIOLOGY LETTERS 308 (1): 48-54.
Smits, T. H. M., Rezzonico, F., Pelludat, C., Goesmann, A., Frey, J. E., and Duffy, B. (2010). Genomic and phenotypic characterization of a nonpigmented variant of Pantoea vagans biocontrol strain C9-1 lacking the 530-kb megaplasmid pPag3. FEMS MICROBIOLOGY LETTERS 308, 48-54.
Smits, T.H.M., et al., 2010. Genomic and phenotypic characterization of a nonpigmented variant of Pantoea vagans biocontrol strain C9-1 lacking the 530-kb megaplasmid pPag3. FEMS MICROBIOLOGY LETTERS, 308(1), p 48-54.
T.H.M. Smits, et al., “Genomic and phenotypic characterization of a nonpigmented variant of Pantoea vagans biocontrol strain C9-1 lacking the 530-kb megaplasmid pPag3”, FEMS MICROBIOLOGY LETTERS, vol. 308, 2010, pp. 48-54.
Smits, T.H.M., Rezzonico, F., Pelludat, C., Goesmann, A., Frey, J.E., Duffy, B.: Genomic and phenotypic characterization of a nonpigmented variant of Pantoea vagans biocontrol strain C9-1 lacking the 530-kb megaplasmid pPag3. FEMS MICROBIOLOGY LETTERS. 308, 48-54 (2010).
Smits, Theo H. M., Rezzonico, Fabio, Pelludat, Cosima, Goesmann, Alexander, Frey, Juerg E., and Duffy, Brion. “Genomic and phenotypic characterization of a nonpigmented variant of Pantoea vagans biocontrol strain C9-1 lacking the 530-kb megaplasmid pPag3”. FEMS MICROBIOLOGY LETTERS 308.1 (2010): 48-54.

11 Zitationen in Europe PMC

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