Transcriptome sequencing and comparative transcriptome analysis of the scleroglucan producer Sclerotium rolfsii
Schmid J, Müller-Hagen D, Bekel T, Funk L, Stahl U, Sieber V, Meyer V (2010)
BMC Genomics 11(1): 329.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
Download
Autor*in
Schmid, Jochen;
Müller-Hagen, Dirk;
Bekel, ThomasUniBi;
Funk, Laura;
Stahl, Ulf;
Sieber, Volker;
Meyer, Vera
Abstract / Bemerkung
Background The plant pathogenic basidiomycete Sclerotium rolfsii produces the industrially exploited exopolysaccharide scleroglucan, a polymer that consists of (1 -> 3)-[beta]-linked glucose with a (1 -> 6)-(beta)-glycosyl branch on every third unit. Although the physicochemical properties of scleroglucan are well understood, almost nothing is known about the genetics of scleroglucan biosynthesis. Similarly, the biosynthetic pathway of oxalate, the main by-product during scleroglucan production, has not been elucidated yet. In order to provide a basis for genetic and metabolic engineering approaches, we studied scleroglucan and oxalate biosynthesis in S. rolfsii using different transcriptomic approaches. Results Two S. rolfsii transcriptomes obtained from scleroglucan-producing and scleroglucan-nonproducing conditions were pooled and sequenced using the 454 pyrosequencing technique yielding ~350,000 reads. These could be assembled into 21,937 contigs and 171,833 singletons, for which 6,951 had significant matches in public protein data bases. Sequence data were used to obtain first insights into the genomics of scleroglucan and oxalate production and to predict putative proteins involved in the synthesis of both metabolites. Using comparative transcriptomics, namely Agilent microarray hybridization and suppression subtractive hybridization, we identified ~800 unigenes which are differently expressed under scleroglucan-producing and non-producing conditions. From these, candidate genes were identified which could represent potential leads for targeted modification of the S. rolfsii metabolism for increased scleroglucan yields. Conclusions The results presented in this paper provide for the first time genomic and transcriptomic data about S. rolfsii and demonstrate the power and usefulness of combined transcriptome sequencing and comparative microarray analysis. The data obtained allowed us to predict the biosynthetic pathways of scleroglucan and oxalate synthesis and to identify important genes putatively involved in determining scleroglucan yields. Moreover, our data establish the first sequence database for S. rolfsii, which allows research into other biological processes of S. rolfsii, such as host-pathogen interaction.
Erscheinungsjahr
2010
Zeitschriftentitel
BMC Genomics
Band
11
Ausgabe
1
Art.-Nr.
329
ISSN
1471-2164
Page URI
https://pub.uni-bielefeld.de/record/1785552
Zitieren
Schmid J, Müller-Hagen D, Bekel T, et al. Transcriptome sequencing and comparative transcriptome analysis of the scleroglucan producer Sclerotium rolfsii. BMC Genomics. 2010;11(1): 329.
Schmid, J., Müller-Hagen, D., Bekel, T., Funk, L., Stahl, U., Sieber, V., & Meyer, V. (2010). Transcriptome sequencing and comparative transcriptome analysis of the scleroglucan producer Sclerotium rolfsii. BMC Genomics, 11(1), 329. https://doi.org/10.1186/1471-2164-11-329
Schmid, Jochen, Müller-Hagen, Dirk, Bekel, Thomas, Funk, Laura, Stahl, Ulf, Sieber, Volker, and Meyer, Vera. 2010. “Transcriptome sequencing and comparative transcriptome analysis of the scleroglucan producer Sclerotium rolfsii”. BMC Genomics 11 (1): 329.
Schmid, J., Müller-Hagen, D., Bekel, T., Funk, L., Stahl, U., Sieber, V., and Meyer, V. (2010). Transcriptome sequencing and comparative transcriptome analysis of the scleroglucan producer Sclerotium rolfsii. BMC Genomics 11:329.
Schmid, J., et al., 2010. Transcriptome sequencing and comparative transcriptome analysis of the scleroglucan producer Sclerotium rolfsii. BMC Genomics, 11(1): 329.
J. Schmid, et al., “Transcriptome sequencing and comparative transcriptome analysis of the scleroglucan producer Sclerotium rolfsii”, BMC Genomics, vol. 11, 2010, : 329.
Schmid, J., Müller-Hagen, D., Bekel, T., Funk, L., Stahl, U., Sieber, V., Meyer, V.: Transcriptome sequencing and comparative transcriptome analysis of the scleroglucan producer Sclerotium rolfsii. BMC Genomics. 11, : 329 (2010).
Schmid, Jochen, Müller-Hagen, Dirk, Bekel, Thomas, Funk, Laura, Stahl, Ulf, Sieber, Volker, and Meyer, Vera. “Transcriptome sequencing and comparative transcriptome analysis of the scleroglucan producer Sclerotium rolfsii”. BMC Genomics 11.1 (2010): 329.
Alle Dateien verfügbar unter der/den folgenden Lizenz(en):
Copyright Statement:
Dieses Objekt ist durch das Urheberrecht und/oder verwandte Schutzrechte geschützt. [...]
Volltext(e)
Name
Access Level
![Open access file OA](https://pub.uni-bielefeld.de/images/access_open.png)
Zuletzt Hochgeladen
2019-09-06T08:49:04Z
MD5 Prüfsumme
bc8c8bdaa837820e5f590a2204a70959
Daten bereitgestellt von European Bioinformatics Institute (EBI)
ARXPR
1 Eintrag gefunden, die diesen Artikel zitieren
E-GEOD-21040 (ARXPR: E-GEOD-21040)
Bibliography accession: E-GEOD-21040 - Transcriptome sequencing and comparative transcriptome analysis of the scleroglucan producer Sclerotium rolfsii
Bibliography accession: E-GEOD-21040 - Transcriptome sequencing and comparative transcriptome analysis of the scleroglucan producer Sclerotium rolfsii
14 Zitationen in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
Comparative Transcriptome Analysis between the Fungal Plant Pathogens Sclerotinia sclerotiorum and S. trifoliorum Using RNA Sequencing.
Qiu D, Xu L, Vandemark G, Chen W., J Hered 107(2), 2016
PMID: 26615185
Qiu D, Xu L, Vandemark G, Chen W., J Hered 107(2), 2016
PMID: 26615185
Identification of genes differentially expressed during early interactions between the stem rot fungus (Sclerotium rolfsii) and peanut (Arachis hypogaea) cultivars with increasing disease resistance levels.
Jogi A, Kerry JW, Brenneman TB, Leebens-Mack JH, Gold SE., Microbiol Res 184(), 2016
PMID: 26856448
Jogi A, Kerry JW, Brenneman TB, Leebens-Mack JH, Gold SE., Microbiol Res 184(), 2016
PMID: 26856448
Transcriptome Analysis Reveals the Genetic Basis of the Resveratrol Biosynthesis Pathway in an Endophytic Fungus (Alternaria sp. MG1) Isolated from Vitis vinifera.
Che J, Shi J, Gao Z, Zhang Y., Front Microbiol 7(), 2016
PMID: 27588016
Che J, Shi J, Gao Z, Zhang Y., Front Microbiol 7(), 2016
PMID: 27588016
Microbial production of scleroglucan and downstream processing.
Castillo NA, Valdez AL, Fariña JI., Front Microbiol 6(), 2015
PMID: 26528259
Castillo NA, Valdez AL, Fariña JI., Front Microbiol 6(), 2015
PMID: 26528259
The sweet branch of metabolic engineering: cherry-picking the low-hanging sugary fruits.
Chen R., Microb Cell Fact 14(), 2015
PMID: 26655367
Chen R., Microb Cell Fact 14(), 2015
PMID: 26655367
Peroxidase gene discovery from the horseradish transcriptome.
Näätsaari L, Krainer FW, Schubert M, Glieder A, Thallinger GG., BMC Genomics 15(), 2014
PMID: 24666710
Näätsaari L, Krainer FW, Schubert M, Glieder A, Thallinger GG., BMC Genomics 15(), 2014
PMID: 24666710
Differences in gene expression within a striking phenotypic mosaic Eucalyptus tree that varies in susceptibility to herbivory.
Padovan A, Lanfear R, Keszei A, Foley WJ, Külheim C., BMC Plant Biol 13(), 2013
PMID: 23421644
Padovan A, Lanfear R, Keszei A, Foley WJ, Külheim C., BMC Plant Biol 13(), 2013
PMID: 23421644
Characterization of Liaoning cashmere goat transcriptome: sequencing, de novo assembly, functional annotation and comparative analysis.
Liu H, Wang T, Wang J, Quan F, Zhang Y., PLoS One 8(10), 2013
PMID: 24130835
Liu H, Wang T, Wang J, Quan F, Zhang Y., PLoS One 8(10), 2013
PMID: 24130835
Tracking nickel-adaptive biomarkers in Pisolithus albus from New Caledonia using a transcriptomic approach.
Majorel C, Hannibal L, Soupe ME, Carriconde F, Ducousso M, Lebrun M, Jourand P., Mol Ecol 21(9), 2012
PMID: 22429322
Majorel C, Hannibal L, Soupe ME, Carriconde F, Ducousso M, Lebrun M, Jourand P., Mol Ecol 21(9), 2012
PMID: 22429322
Isolation of oxalic acid tolerating fungi and decipherization of its potential to control Sclerotinia sclerotiorum through oxalate oxidase like protein.
Yadav S, Srivastava AK, Singh DP, Arora DK., World J Microbiol Biotechnol 28(11), 2012
PMID: 22864600
Yadav S, Srivastava AK, Singh DP, Arora DK., World J Microbiol Biotechnol 28(11), 2012
PMID: 22864600
Isolation of Oxalic acid tolerating fungi and decipherization of its potential to control Sclerotinia sclerotiorum through oxalate oxidase like protein
Yadav S, Srivastava AK, Singh DP, Arora DK., World J Microbiol Biotechnol 28(11), 2012
PMID: IND500589530
Yadav S, Srivastava AK, Singh DP, Arora DK., World J Microbiol Biotechnol 28(11), 2012
PMID: IND500589530
The maternal and early embryonic transcriptome of the milkweed bug Oncopeltus fasciatus.
Ewen-Campen B, Shaner N, Panfilio KA, Suzuki Y, Roth S, Extavour CG., BMC Genomics 12(), 2011
PMID: 21266083
Ewen-Campen B, Shaner N, Panfilio KA, Suzuki Y, Roth S, Extavour CG., BMC Genomics 12(), 2011
PMID: 21266083
Scleroglucan: biosynthesis, production and application of a versatile hydrocolloid.
Schmid J, Meyer V, Sieber V., Appl Microbiol Biotechnol 91(4), 2011
PMID: 21732244
Schmid J, Meyer V, Sieber V., Appl Microbiol Biotechnol 91(4), 2011
PMID: 21732244
Comparing de novo assemblers for 454 transcriptome data.
Kumar S, Blaxter ML., BMC Genomics 11(), 2010
PMID: 20950480
Kumar S, Blaxter ML., BMC Genomics 11(), 2010
PMID: 20950480
53 References
Daten bereitgestellt von Europe PubMed Central.
Notes on the parasitology of Sclerotium rolfsii
AUTHOR UNKNOWN, 1928
AUTHOR UNKNOWN, 1928
Corticium rolfsii
AUTHOR UNKNOWN, 1974
AUTHOR UNKNOWN, 1974
Collection and determination of root and stem rot pathogens
AUTHOR UNKNOWN, 1996
AUTHOR UNKNOWN, 1996
Scleroglucan
AUTHOR UNKNOWN, 1993
AUTHOR UNKNOWN, 1993
Exocellular, microbial polysaccharides.
Sandford PA., Adv Carbohydr Chem Biochem 36(), 1979
PMID: 380276
Sandford PA., Adv Carbohydr Chem Biochem 36(), 1979
PMID: 380276
Influence of nonionic surfactant on aggregation state of scleroglucan in aqueous solution.
El Ouriaghli T, Francois J, Sarazin D, Truong DN., Carbohydrate polymers. 17(4), 1992
PMID: IND20374667
El Ouriaghli T, Francois J, Sarazin D, Truong DN., Carbohydrate polymers. 17(4), 1992
PMID: IND20374667
Scleroglucan: Fermentative Production, Downstream Processing and Applications
AUTHOR UNKNOWN, 2007
AUTHOR UNKNOWN, 2007
Structural stability of Sclerotium rolfsii ATCC 201126 beta-glucan with fermentation time: a chemical, infrared spectroscopic and enzymatic approach.
Farina JI, Vinarta SC, Cattaneo M, Figueroa LI., J. Appl. Microbiol. 106(1), 2008
PMID: 19054236
Farina JI, Vinarta SC, Cattaneo M, Figueroa LI., J. Appl. Microbiol. 106(1), 2008
PMID: 19054236
A new polysaccharidic gel matrix for drug delivery: preparation and mechanical properties.
Coviello T, Alhaique F, Parisi C, Matricardi P, Bocchinfuso G, Grassi M., J Control Release 102(3), 2005
PMID: 15681086
Coviello T, Alhaique F, Parisi C, Matricardi P, Bocchinfuso G, Grassi M., J Control Release 102(3), 2005
PMID: 15681086
A new scleroglucan/borax hydrogel: swelling and drug release studies.
Coviello T, Grassi M, Palleschi A, Bocchinfuso G, Coluzzi G, Banishoeib F, Alhaique F., Int J Pharm 289(1-2), 2004
PMID: 15652203
Coviello T, Grassi M, Palleschi A, Bocchinfuso G, Coluzzi G, Banishoeib F, Alhaique F., Int J Pharm 289(1-2), 2004
PMID: 15652203
Scleroglucan
AUTHOR UNKNOWN, 2002
AUTHOR UNKNOWN, 2002
Microbial polysaccharides-a comparison with eukaryotic polymers
AUTHOR UNKNOWN, 1989
AUTHOR UNKNOWN, 1989
Structure and assembly of epiglucan, the extracellular (1-->3;1-->6)-beta-glucan produced by the fungus Epicoccum nigrum strain F19.
Schmid F, Stone BA, Brownlee RT, McDougall BM, Seviour RJ., Carbohydr. Res. 341(3), 2005
PMID: 16359651
Schmid F, Stone BA, Brownlee RT, McDougall BM, Seviour RJ., Carbohydr. Res. 341(3), 2005
PMID: 16359651
Biosynthesis of the [beta]--glucan of Sclerotium rolfsii sacc. Direction of chain propagation and the insertion of the branch residues
AUTHOR UNKNOWN, 1969
AUTHOR UNKNOWN, 1969
High scleroglucan production by Sclerotium rolfsii: Influence of medium composition
AUTHOR UNKNOWN, 1998
AUTHOR UNKNOWN, 1998
Influence of carbon source and pH on oxalate accumulation in culture filtrates of S. rolfsii
AUTHOR UNKNOWN, 1965
AUTHOR UNKNOWN, 1965
Scleroglucan and oxalic acid formation by Sclerotium glucanicum in sucrose supplemented fermentation
AUTHOR UNKNOWN, 1994
AUTHOR UNKNOWN, 1994
AUTHOR UNKNOWN, 1998
Repression of oxalic acid biosynthesis in the unsterile scleroglucan production process with Sclerotium rolfsii ATCC 15205
AUTHOR UNKNOWN, 2000
AUTHOR UNKNOWN, 2000
Enhanced production of scleroglucan by Sclerotium rolfsii MTCC 2156 by use of metabolic precursors.
Survase SA, Saudagar PS, Singhal RS., Bioresour. Technol. 98(2), 2006
PMID: 16806909
Survase SA, Saudagar PS, Singhal RS., Bioresour. Technol. 98(2), 2006
PMID: 16806909
Use of complex media for the production of scleroglucan by Sclerotium rolfsii MTCC 2156.
Survase SA, Saudagar PS, Singhal RS., Bioresour. Technol. 98(7), 2006
PMID: 16822667
Survase SA, Saudagar PS, Singhal RS., Bioresour. Technol. 98(7), 2006
PMID: 16822667
SIMULTANEOUS PRODUCTION AND SYNERGISTIC ACTION OF OXALIC ACID AND POLYGALACTURONASE DURING PATHOGENESIS BY SCLEROTIUM ROLFSII.
BATEMAN DF, BEER SV., Phytopathology 55(), 1965
PMID: 14274523
BATEMAN DF, BEER SV., Phytopathology 55(), 1965
PMID: 14274523
Oxalate production by fungi: its role in pathogenicity and ecology in the soil environment
AUTHOR UNKNOWN, 1996
AUTHOR UNKNOWN, 1996
Low molecular weight chelators and phenolic compounds isolated from wood decay fungi and their role in the fungal biodegradation of wood
AUTHOR UNKNOWN, 1995
AUTHOR UNKNOWN, 1995
A physiological role for oxalic acid biosynthesis in the wood-rotting basidiomycete Fomitopsis palustris.
Munir E, Yoon JJ, Tokimatsu T, Hattori T, Shimada M., Proc. Natl. Acad. Sci. U.S.A. 98(20), 2001
PMID: 11553780
Munir E, Yoon JJ, Tokimatsu T, Hattori T, Shimada M., Proc. Natl. Acad. Sci. U.S.A. 98(20), 2001
PMID: 11553780
Possible biochemical roles of oxalic acid as a low molecular weight compound involved in brown-rot and white-rot wood decays
AUTHOR UNKNOWN, 2007
AUTHOR UNKNOWN, 2007
Induction of polygalacturonase and the formation of oxalic acid by pectin in brown-rot fungi.
Green FIII, Clausen CA, Kuster TA, Highley TL., World J. Microbiol. Biotechnol. 11(5), 1995
PMID: IND20518631
Green FIII, Clausen CA, Kuster TA, Highley TL., World J. Microbiol. Biotechnol. 11(5), 1995
PMID: IND20518631
Hydrolysis of bordered pits during colonization of conifers by brown-rot decay
AUTHOR UNKNOWN, 1995
AUTHOR UNKNOWN, 1995
Anion channel activity is necessary to induce ethylene synthesis and programmed cell death in response to oxalic acid.
Errakhi R, Meimoun P, Lehner A, Vidal G, Briand J, Corbineau F, Rona JP, Bouteau F., J. Exp. Bot. 59(11), 2008
PMID: 18612171
Errakhi R, Meimoun P, Lehner A, Vidal G, Briand J, Corbineau F, Rona JP, Bouteau F., J. Exp. Bot. 59(11), 2008
PMID: 18612171
Toxic and signalling effects of oxalic acid: Oxalic acid-Natural born killer or natural born protector?
Lehner A, Meimoun P, Errakhi R, Madiona K, Barakate M, Bouteau F., Plant Signal Behav 3(9), 2008
PMID: 19704845
Lehner A, Meimoun P, Errakhi R, Madiona K, Barakate M, Bouteau F., Plant Signal Behav 3(9), 2008
PMID: 19704845
Oxalic acid is an elicitor of plant programmed cell death during Sclerotinia sclerotiorum disease development.
Kim KS, Min JY, Dickman MB., Mol. Plant Microbe Interact. 21(5), 2008
PMID: 18393620
Kim KS, Min JY, Dickman MB., Mol. Plant Microbe Interact. 21(5), 2008
PMID: 18393620
The Sequence Analysis and Management System -- SAMS-2.0: data management and sequence analysis adapted to changing requirements from traditional sanger sequencing to ultrafast sequencing technologies.
Bekel T, Henckel K, Kuster H, Meyer F, Mittard Runte V, Neuweger H, Paarmann D, Rupp O, Zakrzewski M, Puhler A, Stoye J, Goesmann A., J. Biotechnol. 140(1-2), 2009
PMID: 19297685
Bekel T, Henckel K, Kuster H, Meyer F, Mittard Runte V, Neuweger H, Paarmann D, Rupp O, Zakrzewski M, Puhler A, Stoye J, Goesmann A., J. Biotechnol. 140(1-2), 2009
PMID: 19297685
Basic local alignment search tool.
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ., J. Mol. Biol. 215(3), 1990
PMID: 2231712
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ., J. Mol. Biol. 215(3), 1990
PMID: 2231712
Evidence for a cytoplasmic pathway of oxalate biosynthesis in Aspergillus niger
AUTHOR UNKNOWN, 1988
AUTHOR UNKNOWN, 1988
Oxaloacetate hydrolase, the C-C bond lyase of oxalate secreting fungi.
Han Y, Joosten HJ, Niu W, Zhao Z, Mariano PS, McCalman M, van Kan J, Schaap PJ, Dunaway-Mariano D., J. Biol. Chem. 282(13), 2007
PMID: 17244616
Han Y, Joosten HJ, Niu W, Zhao Z, Mariano PS, McCalman M, van Kan J, Schaap PJ, Dunaway-Mariano D., J. Biol. Chem. 282(13), 2007
PMID: 17244616
Production of calcium oxalate crystals by the basidiomycete Moniliophthora perniciosa, the causal agent of witches' broom disease of Cacao.
Rio MC, de Oliveira BV, de Tomazella DP, Silva JA, Pereira GA., Curr. Microbiol. 56(4), 2008
PMID: 18172716
Rio MC, de Oliveira BV, de Tomazella DP, Silva JA, Pereira GA., Curr. Microbiol. 56(4), 2008
PMID: 18172716
Oxalic acid biosynthesis by Sclerotium rolfsii
AUTHOR UNKNOWN, 1968
AUTHOR UNKNOWN, 1968
Transcriptional control of nitric oxide reductase gene (CYP55) in the fungal denitrifier Fusarium oxysporum.
Takaya N, Uchimura H, Lai Y, Shoun H., Biosci. Biotechnol. Biochem. 66(5), 2002
PMID: 12092813
Takaya N, Uchimura H, Lai Y, Shoun H., Biosci. Biotechnol. Biochem. 66(5), 2002
PMID: 12092813
Nitrate reductase-formate dehydrogenase couple involved in the fungal denitrification by Fusarium oxysporum.
Uchimura H, Enjoji H, Seki T, Taguchi A, Takaya N, Shoun H., J. Biochem. 131(4), 2002
PMID: 11926996
Uchimura H, Enjoji H, Seki T, Taguchi A, Takaya N, Shoun H., J. Biochem. 131(4), 2002
PMID: 11926996
Cloning and sequencing of two Ceriporiopsis subvermispora bicupin oxalate oxidase allelic isoforms: implications for the reaction specificity of oxalate oxidases and decarboxylases.
Escutia MR, Bowater L, Edwards A, Bottrill AR, Burrell MR, Polanco R, Vicuna R, Bornemann S., Appl. Environ. Microbiol. 71(7), 2005
PMID: 16000768
Escutia MR, Bowater L, Edwards A, Bottrill AR, Burrell MR, Polanco R, Vicuna R, Bornemann S., Appl. Environ. Microbiol. 71(7), 2005
PMID: 16000768
Cloning and characterization of the 5'-flanking region of the oxalate decarboxylase gene from Flammulina velutipes.
Azam M, Kesarwani M, Chakraborty S, Natarajan K, Datta A., Biochem. J. 367(Pt 1), 2002
PMID: 12020349
Azam M, Kesarwani M, Chakraborty S, Natarajan K, Datta A., Biochem. J. 367(Pt 1), 2002
PMID: 12020349
The FunCat, a functional annotation scheme for systematic classification of proteins from whole genomes.
Ruepp A, Zollner A, Maier D, Albermann K, Hani J, Mokrejs M, Tetko I, Guldener U, Mannhaupt G, Munsterkotter M, Mewes HW., Nucleic Acids Res. 32(18), 2004
PMID: 15486203
Ruepp A, Zollner A, Maier D, Albermann K, Hani J, Mokrejs M, Tetko I, Guldener U, Mannhaupt G, Munsterkotter M, Mewes HW., Nucleic Acids Res. 32(18), 2004
PMID: 15486203
Effects of salt stress on carbohydrate metabolism in desert soil alga Microcoleus vaginatus Gom
AUTHOR UNKNOWN, 2006
AUTHOR UNKNOWN, 2006
The correlation between salt tolerance and extracellular polysaccharide production in Rhizobium
AUTHOR UNKNOWN, 1997
AUTHOR UNKNOWN, 1997
Modification of exopolysaccharide composition and production by three cyanobacterial isolates under salt stress.
Ozturk S, Aslim B., Environ Sci Pollut Res Int 17(3), 2009
PMID: 19727881
Ozturk S, Aslim B., Environ Sci Pollut Res Int 17(3), 2009
PMID: 19727881
Identification of salt stress inducible genes that control cell envelope related functions in Azospirillum brasilense Sp7.
Nagarajan T, Vanderleyden J, Tripathi AK., Mol. Genet. Genomics 278(1), 2007
PMID: 17340145
Nagarajan T, Vanderleyden J, Tripathi AK., Mol. Genet. Genomics 278(1), 2007
PMID: 17340145
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ., Biochemistry 18(24), 1979
PMID: 518835
Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ., Biochemistry 18(24), 1979
PMID: 518835
Oxalate oxidases and differentiating surface structure in wheat: germins.
Lane BG., Biochem. J. 349(Pt 1), 2000
PMID: 10861243
Lane BG., Biochem. J. 349(Pt 1), 2000
PMID: 10861243
Germin, a protein marker of early plant development, is an oxalate oxidase.
Lane BG, Dunwell JM, Ray JA, Schmitt MR, Cuming AC., J. Biol. Chem. 268(17), 1993
PMID: 8509360
Lane BG, Dunwell JM, Ray JA, Schmitt MR, Cuming AC., J. Biol. Chem. 268(17), 1993
PMID: 8509360
Tissue-Specific Expression of Germin-Like Oxalate Oxidase during Development and Fungal Infection of Barley Seedlings.
Dumas B, Freyssinet G, Pallett KE., Plant Physiol. 107(4), 1995
PMID: 12228419
Dumas B, Freyssinet G, Pallett KE., Plant Physiol. 107(4), 1995
PMID: 12228419
Oxalate decarboxylase in the brown-rot wood decay fungus Postia placenta
AUTHOR UNKNOWN, 1995
AUTHOR UNKNOWN, 1995
Transformation of Aspergillus nidulans by using a trpC plasmid.
Yelton MM, Hamer JE, Timberlake WE., Proc. Natl. Acad. Sci. U.S.A. 81(5), 1984
PMID: 6324193
Yelton MM, Hamer JE, Timberlake WE., Proc. Natl. Acad. Sci. U.S.A. 81(5), 1984
PMID: 6324193
Export
Markieren/ Markierung löschen
Markierte Publikationen
Web of Science
Dieser Datensatz im Web of Science®Quellen
PMID: 20504312
PubMed | Europe PMC
Suchen in