Comparative gene expression in toxic versus non-toxic strains of the marine dinoflagellate Alexandrium minutum

Yang I, John U, Beszteri S, Glöckner G, Krock B, Goesmann A, Cembella AD (2010)
BMC Genomics 11(1): 248.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
Yang, Ines; John, Uwe; Beszteri, Sára; Glöckner, Gernot; Krock, Bernd; Goesmann, AlexanderUniBi ; Cembella, Allan D.
Abstract / Bemerkung
Background The dinoflagellate Alexandrium minutum typically produces paralytic shellfish poisoning (PSP) toxins, which are known only from cyanobacteria and dinoflagellates. While a PSP toxin gene cluster has recently been characterized in cyanobacteria, the genetic background of PSP toxin production in dinoflagellates remains elusive. Results We constructed and analysed an expressed sequence tag (EST) library of A. minutum, which contained 15,703 read sequences yielding a total of 4,320 unique expressed clusters. Of these clusters, 72% combined the forward-and reverse reads of at least one bacterial clone. This sequence resource was then used to construct an oligonucleotide microarray. We analysed the expression of all clusters in three different strains. While the cyanobacterial PSP toxin genes were not found among the A. minutum sequences, 192 genes were differentially expressed between toxic and non-toxic strains. Conclusions Based on this study and on the lack of identified PSP synthesis genes in the two existent Alexandrium tamarense EST libraries, we propose that the PSP toxin genes in dinoflagellates might be more different from their cyanobacterial counterparts than would be expected in the case of a recent gene transfer. As a starting point to identify possible PSP toxin-associated genes in dinoflagellates without relying on a priori sequence information, the sequences only present in mRNA pools of the toxic strain can be seen as putative candidates involved in toxin synthesis and regulation, or acclimation to intracellular PSP toxins.
BMC Genomics
Page URI


Yang I, John U, Beszteri S, et al. Comparative gene expression in toxic versus non-toxic strains of the marine dinoflagellate Alexandrium minutum. BMC Genomics. 2010;11(1): 248.
Yang, I., John, U., Beszteri, S., Glöckner, G., Krock, B., Goesmann, A., & Cembella, A. D. (2010). Comparative gene expression in toxic versus non-toxic strains of the marine dinoflagellate Alexandrium minutum. BMC Genomics, 11(1), 248.
Yang, Ines, John, Uwe, Beszteri, Sára, Glöckner, Gernot, Krock, Bernd, Goesmann, Alexander, and Cembella, Allan D. 2010. “Comparative gene expression in toxic versus non-toxic strains of the marine dinoflagellate Alexandrium minutum”. BMC Genomics 11 (1): 248.
Yang, I., John, U., Beszteri, S., Glöckner, G., Krock, B., Goesmann, A., and Cembella, A. D. (2010). Comparative gene expression in toxic versus non-toxic strains of the marine dinoflagellate Alexandrium minutum. BMC Genomics 11:248.
Yang, I., et al., 2010. Comparative gene expression in toxic versus non-toxic strains of the marine dinoflagellate Alexandrium minutum. BMC Genomics, 11(1): 248.
I. Yang, et al., “Comparative gene expression in toxic versus non-toxic strains of the marine dinoflagellate Alexandrium minutum”, BMC Genomics, vol. 11, 2010, : 248.
Yang, I., John, U., Beszteri, S., Glöckner, G., Krock, B., Goesmann, A., Cembella, A.D.: Comparative gene expression in toxic versus non-toxic strains of the marine dinoflagellate Alexandrium minutum. BMC Genomics. 11, : 248 (2010).
Yang, Ines, John, Uwe, Beszteri, Sára, Glöckner, Gernot, Krock, Bernd, Goesmann, Alexander, and Cembella, Allan D. “Comparative gene expression in toxic versus non-toxic strains of the marine dinoflagellate Alexandrium minutum”. BMC Genomics 11.1 (2010): 248.
Alle Dateien verfügbar unter der/den folgenden Lizenz(en):
Copyright Statement:
Dieses Objekt ist durch das Urheberrecht und/oder verwandte Schutzrechte geschützt. [...]
Access Level
OA Open Access
Zuletzt Hochgeladen
MD5 Prüfsumme

16 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Gene Expression Variation Resolves Species and Individual Strains among Coral-Associated Dinoflagellates within the Genus Symbiodinium.
Parkinson JE, Baumgarten S, Michell CT, Baums IB, LaJeunesse TC, Voolstra CR., Genome Biol Evol 8(3), 2016
PMID: 26868597
Low dose extended exposure to saxitoxin and its potential neurodevelopmental effects: A review.
O'Neill K, Musgrave IF, Humpage A., Environ Toxicol Pharmacol 48(), 2016
PMID: 27716534
Transcriptome de novo assembly sequencing and analysis of the toxic dinoflagellate Alexandrium catenella using the Illumina platform.
Zhang S, Sui Z, Chang L, Kang K, Ma J, Kong F, Zhou W, Wang J, Guo L, Geng H, Zhong J, Ma Q., Gene 537(2), 2014
PMID: 24440238
Bacterial community affects toxin production by Gymnodinium catenatum.
Albinsson ME, Negri AP, Blackburn SI, Bolch CJ., PLoS One 9(8), 2014
PMID: 25117053
An analysis of dinoflagellate metabolism using EST data.
Butterfield ER, Howe CJ, Nisbet RE., Protist 164(2), 2013
PMID: 23085481
The globally distributed genus Alexandrium: multifaceted roles in marine ecosystems and impacts on human health.
Anderson DM, Alpermann TJ, Cembella AD, Collos Y, Masseret E, Montresor M., Harmful Algae 14(), 2012
PMID: 22308102
Distribution and genetic diversity of the luciferase gene within marine dinoflagellates1
Valiadi M, Debora Iglesias‐Rodriguez M, Amorim A., J Phycol 48(3), 2012
PMID: IND44783336
Discovery of nuclear-encoded genes for the neurotoxin saxitoxin in dinoflagellates.
Stüken A, Orr RJ, Kellmann R, Murray SA, Neilan BA, Jakobsen KS., PLoS One 6(5), 2011
PMID: 21625593
sxtA-based quantitative molecular assay to identify saxitoxin-producing harmful algal blooms in marine waters.
Murray SA, Wiese M, Stüken A, Brett S, Kellmann R, Hallegraeff G, Neilan BA., Appl Environ Microbiol 77(19), 2011
PMID: 21841034
The relevance of marine chemical ecology to plankton and ecosystem function: an emerging field.
Ianora A, Bentley MG, Caldwell GS, Casotti R, Cembella AD, Engström-Öst J, Halsband C, Sonnenschein E, Legrand C, Llewellyn CA, Paldavičienë A, Pilkaityte R, Pohnert G, Razinkovas A, Romano G, Tillmann U, Vaiciute D., Mar Drugs 9(9), 2011
PMID: 22131962
The transcriptome of the novel dinoflagellate Oxyrrhis marina (Alveolata: Dinophyceae): response to salinity examined by 454 sequencing.
Lowe CD, Mello LV, Samatar N, Martin LE, Montagnes DJ, Watts PC., BMC Genomics 12(), 2011
PMID: 22014029
Comparative genomic and transcriptomic characterization of the toxigenic marine dinoflagellate Alexandrium ostenfeldii.
Jaeckisch N, Yang I, Wohlrab S, Glöckner G, Kroymann J, Vogel H, Cembella A, John U., PLoS One 6(12), 2011
PMID: 22164224
Neurotoxic alkaloids: saxitoxin and its analogs.
Wiese M, D'Agostino PM, Mihali TK, Moffitt MC, Neilan BA., Mar Drugs 8(7), 2010
PMID: 20714432

88 References

Daten bereitgestellt von Europe PubMed Central.

Phylogeny, biogeography, and species boundaries within the Alexandrium minutum group
Humpback whales (Megaptera novaeangliae) fatally poisoned by dinoflagellate toxin
High affinity binding of red tide neurotoxins to marine mammal brain
Purification and characterization of a sulfotransferase specific to N-21 of saxitoxin and gonyautoxin 2+3 from the toxic dinoflagellate Gymnodinium catenatum (Dinophyceae)
Toxin profile of Alexandrium catenella from the Chilean coast as determined by liquid chromatography with fluorescence detection and liquid chromatography coupled with tandem mass spectrometry
Ecophysiology and metabolism of paralytic shellfish toxins in marine microalgae
Notes on Alexandrium population dynamics
Phylogenetic diversity and specificity of bacteria closely associated with Alexandrium spp. and other phytoplankton.
Jasti S, Sieracki ME, Poulton NJ, Giewat MW, Rooney-Varga JN., Appl. Environ. Microbiol. 71(7), 2005
PMID: 16000752
Toxic marine phytoplankton, zooplankton grazers, and pelagic food webs
Copepods induce paralytic shellfish toxin production in marine dinoflagellates.
Selander E, Thor P, Toth G, Pavia H., Proc. Biol. Sci. 273(1594), 2006
PMID: 16769640
Ecological advantages of toxin production by the dinoflagellate Alexandrium minutum under phosphorus limitation
Allelochemical interactions and short-term effects of the dinoflagellate Alexandrium on selected photoautotrophic and heterotrophic protists
Toxic effects of Alexandrium spp. on heterotrophic dinoflagellates: an allelochemical defence mechanism independent of PSP-toxin content
Molecular physiology of toxin production and growth regulation in harmful algae
Dinoflagellate nuclear SSU rRNA phylogeny suggests multiple plastid losses and replacements.
Saldarriaga JF, Taylor FJ, Keeling PJ, Cavalier-Smith T., J. Mol. Evol. 53(3), 2001
PMID: 11523007
Effect of associated bacteria on the growth and toxicity of Alexandrium catenella.
Uribe P, Espejo RT., Appl. Environ. Microbiol. 69(1), 2003
PMID: 12514056
Genetic and biochemical approaches to PSP toxin production of toxic dinoflagellates
Biosynthesis of saxitoxin analogs: the unexpected pathway
Microalgal metabolites: a new perspective.
Shimizu Y., Annu. Rev. Microbiol. 50(), 1996
PMID: 8905087
Biosynthetic intermediate analysis and functional homology reveal a saxitoxin gene cluster in cyanobacteria.
Kellmann R, Mihali TK, Jeon YJ, Pickford R, Pomati F, Neilan BA., Appl. Environ. Microbiol. 74(13), 2008
PMID: 18487408
The smallest known genomes of multicellular and toxic cyanobacteria: comparison, minimal gene sets for linked traits and the evolutionary implications.
Stucken K, John U, Cembella A, Murillo AA, Soto-Liebe K, Fuentes-Valdes JJ, Friedel M, Plominsky AM, Vasquez M, Glockner G., PLoS ONE 5(2), 2010
PMID: 20169071
Identification of a saxitoxin biosynthesis gene with a history of frequent horizontal gene transfers.
Kellmann R, Mihali TK, Michali TK, Neilan BA, Neilan BA., J. Mol. Evol. 67(5), 2008
PMID: 18850059
Toxin variability during the cell cycle of the dinoflagellate Alexandrium fundyense
Non-toxic and toxic subclones obtained from a toxic clonal culture of Alexandrium tamarense (Dinophyceae): Toxicity and molecular biological feature
Symbiodinium (Pyrrhophyta) genome sizes (DNA content) are smallest among dinoflagellates
Organization of the genome and gene expression in a nuclear environment lacking histones and nucleosomes: the amazing dinoflagellates.
Moreno Diaz de la Espina S, Alverca E, Cuadrado A, Franca S., Eur. J. Cell Biol. 84(2-3), 2005
PMID: 15819396
Nuclear genome sequence survey of the Dinoflagellate Heterocapsa triquetra.
McEwan M, Humayun R, Slamovits CH, Keeling PJ., J. Eukaryot. Microbiol. 55(6), 2008
PMID: 19120799
Insights into a dinoflagellate genome through expressed sequence tag analysis.
Hackett JD, Scheetz TE, Yoon HS, Soares MB, Bonaldo MF, Casavant TL, Bhattacharya D., BMC Genomics 6(), 2005
PMID: 15921535
Genomic characterization of the spirolide-producing dinoflagellate Alexandrium ostenfeldii with special emphasis on PKS genes
Preparation and analysis of an expressed sequence tag library from the toxic dinoflagellate Alexandrium catenella.
Uribe P, Fuentes D, Valdes J, Shmaryahu A, Zuniga A, Holmes D, Valenzuela PD., Mar. Biotechnol. 10(6), 2008
PMID: 18478293
Complex protein targeting to dinoflagellate plastids.
Patron NJ, Waller RF, Archibald JM, Keeling PJ., J. Mol. Biol. 348(4), 2005
PMID: 15843030
Expressed sequence tag analysis of the dinoflagellate Lingulodinium polyedrum during dark phase.
Tanikawa N, Akimoto H, Ogoh K, Chun W, Ohmiya Y., Photochem. Photobiol. 80(), 2004
PMID: 15339226
Novel dinoflagellate clock-related genes identified through microarray analysis
Microarray analysis of diurnal- and circadian-regulated genes in the Florida red-tide dinoflagellate Karenia brevis (Dinophyceae)
Van Dolah FM, Lidie KB, Morey JS, Brunelle SA, Ryan JC, Monroe EA, Haynes BL., J. Phycol. 43(4), 2007
PMID: IND43937731
Novel insights into evolution of protistan polyketide synthases through phylogenomic analysis.
John U, Beszteri B, Derelle E, Van de Peer Y, Read B, Moreau H, Cembella A., Protist 159(1), 2007
PMID: 17931970
Media for the culture of oceanic ultraphytoplankton
Culture of marine phytoplankton for feeding marine invertebrates
OrfPredictor: predicting protein-coding regions in EST-derived sequences.
Min XJ, Butler G, Storms R, Tsang A., Nucleic Acids Res. 33(Web Server issue), 2005
PMID: 15980561
PhyloGena--a user-friendly system for automated phylogenetic annotation of unknown sequences.
Hanekamp K, Bohnebeck U, Beszteri B, Valentin K., Bioinformatics 23(7), 2007
PMID: 17332025
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ., Nucleic Acids Res. 25(17), 1997
PMID: 9254694
CLC Sequence Viewer. A/S Cb, version 6.0.2
An improved general amino acid replacement matrix.
Le SQ, Gascuel O., Mol. Biol. Evol. 25(7), 2008
PMID: 18367465
Cell cycle dependent expression of toxicity by the ichthyotoxic prymnesiophyte Chrysochromulina polylepis
Spirolide production and photoperiod-dependent growth of the marine dinoflagellate Alexandrium ostenfeldii
Alexandrium tamutum sp nov (Dinophyceae): A new nontoxic species in the genus Alexandrium
Significance analysis of microarrays applied to the ionizing radiation response.
Tusher VG, Tibshirani R, Chu G., Proc. Natl. Acad. Sci. U.S.A. 98(9), 2001
PMID: 11309499
TM4 microarray software suite.
Saeed AI, Bhagabati NK, Braisted JC, Liang W, Sharov V, Howe EA, Li J, Thiagarajan M, White JA, Quackenbush J., Meth. Enzymol. 411(), 2006
PMID: 16939790
The positive false discovery rate: A Bayesian interpretation and the q-value
Molecular evolution of the Alexandrium tamarense species complex (Dinophyceae)-dispersal in the North American and West Pacific regions
Spliced leader RNA trans-splicing in dinoflagellates.
Zhang H, Hou Y, Miranda L, Campbell DA, Sturm NR, Gaasterland T, Lin S., Proc. Natl. Acad. Sci. U.S.A. 104(11), 2007
PMID: 17360573
Spliced leader RNA-mediated trans-splicing in a dinoflagellate, Karenia brevis.
Lidie KB, van Dolah FM., J. Eukaryot. Microbiol. 54(5), 2007
PMID: 17910687
Nuclear-encoded, plastid-targeted genes suggest a single common origin for apicomplexan and dinoflagellate plastids.
Fast NM, Kissinger JC, Roos DS, Keeling PJ., Mol. Biol. Evol. 18(3), 2001
PMID: 11230543
Dinoflagellates come from outer space but haptophytes and diatoms do not
Topology of splicing and snRNP biogenesis in dinoflagellate nuclei.
Alverca E, Franca S, Diaz de la Espina SM., Biol. Cell 98(12), 2006
PMID: 16875467
Chemical and enzymatic transformation of paralytic shellfish toxins in marine organisms
A sulfotransferase specific to N-21 of gonyautoxin 2/3 from crude enzyme extraction of toxic dinoflagellate Alexandrium tamarense CI01
Purification and characterization of sulfotransferase specific to O-22 of 11-hydroxy saxitoxin from the toxic dinoflagellate Gymnodinium catenatum (Dinophyceae)
Evolution of filamentous plant pathogens: gene exchange across eukaryotic kingdoms.
Richards TA, Dacks JB, Jenkinson JM, Thornton CR, Talbot NJ., Curr. Biol. 16(18), 2006
PMID: 16979565
Initial sequencing and analysis of the human genome.
Lander ES, Linton LM, Birren B, Nusbaum C, Zody MC, Baldwin J, Devon K, Dewar K, Doyle M, FitzHugh W, Funke R, Gage D, Harris K, Heaford A, Howland J, Kann L, Lehoczky J, LeVine R, McEwan P, McKernan K, Meldrim J, Mesirov JP, Miranda C, Morris W, Naylor J, Raymond C, Rosetti M, Santos R, Sheridan A, Sougnez C, Stange-Thomann Y, Stojanovic N, Subramanian A, Wyman D, Rogers J, Sulston J, Ainscough R, Beck S, Bentley D, Burton J, Clee C, Carter N, Coulson A, Deadman R, Deloukas P, Dunham A, Dunham I, Durbin R, French L, Grafham D, Gregory S, Hubbard T, Humphray S, Hunt A, Jones M, Lloyd C, McMurray A, Matthews L, Mercer S, Milne S, Mullikin JC, Mungall A, Plumb R, Ross M, Shownkeen R, Sims S, Waterston RH, Wilson RK, Hillier LW, McPherson JD, Marra MA, Mardis ER, Fulton LA, Chinwalla AT, Pepin KH, Gish WR, Chissoe SL, Wendl MC, Delehaunty KD, Miner TL, Delehaunty A, Kramer JB, Cook LL, Fulton RS, Johnson DL, Minx PJ, Clifton SW, Hawkins T, Branscomb E, Predki P, Richardson P, Wenning S, Slezak T, Doggett N, Cheng JF, Olsen A, Lucas S, Elkin C, Uberbacher E, Frazier M, Gibbs RA, Muzny DM, Scherer SE, Bouck JB, Sodergren EJ, Worley KC, Rives CM, Gorrell JH, Metzker ML, Naylor SL, Kucherlapati RS, Nelson DL, Weinstock GM, Sakaki Y, Fujiyama A, Hattori M, Yada T, Toyoda A, Itoh T, Kawagoe C, Watanabe H, Totoki Y, Taylor T, Weissenbach J, Heilig R, Saurin W, Artiguenave F, Brottier P, Bruls T, Pelletier E, Robert C, Wincker P, Smith DR, Doucette-Stamm L, Rubenfield M, Weinstock K, Lee HM, Dubois J, Rosenthal A, Platzer M, Nyakatura G, Taudien S, Rump A, Yang H, Yu J, Wang J, Huang G, Gu J, Hood L, Rowen L, Madan A, Qin S, Davis RW, Federspiel NA, Abola AP, Proctor MJ, Myers RM, Schmutz J, Dickson M, Grimwood J, Cox DR, Olson MV, Kaul R, Raymond C, Shimizu N, Kawasaki K, Minoshima S, Evans GA, Athanasiou M, Schultz R, Roe BA, Chen F, Pan H, Ramser J, Lehrach H, Reinhardt R, McCombie WR, de la Bastide M, Dedhia N, Blocker H, Hornischer K, Nordsiek G, Agarwala R, Aravind L, Bailey JA, Bateman A, Batzoglou S, Birney E, Bork P, Brown DG, Burge CB, Cerutti L, Chen HC, Church D, Clamp M, Copley RR, Doerks T, Eddy SR, Eichler EE, Furey TS, Galagan J, Gilbert JG, Harmon C, Hayashizaki Y, Haussler D, Hermjakob H, Hokamp K, Jang W, Johnson LS, Jones TA, Kasif S, Kaspryzk A, Kennedy S, Kent WJ, Kitts P, Koonin EV, Korf I, Kulp D, Lancet D, Lowe TM, McLysaght A, Mikkelsen T, Moran JV, Mulder N, Pollara VJ, Ponting CP, Schuler G, Schultz J, Slater G, Smit AF, Stupka E, Szustakowki J, Thierry-Mieg D, Thierry-Mieg J, Wagner L, Wallis J, Wheeler R, Williams A, Wolf YI, Wolfe KH, Yang SP, Yeh RF, Collins F, Guyer MS, Peterson J, Felsenfeld A, Wetterstrand KA, Patrinos A, Morgan MJ, de Jong P, Catanese JJ, Osoegawa K, Shizuya H, Choi S, Chen YJ, Szustakowki J; International Human Genome Sequencing Consortium., Nature 409(6822), 2001
PMID: 11237011
Genome-wide analysis of redox-regulated genes in a dinoflagellate.
Okamoto OK, Hastings JW., Gene 321(), 2003
PMID: 14636994
Cell cycle behavior of laboratory and field populations of the Florida red tide dinoflagellate, Karenia brevis
Cell division in the dinoflagellate Gamierdiscus toxicus is phased to the diurnal cycle and accompanied by activation of the cell cycle regulatory protein, CDC2 kinase
Evidence for cAMP-dependent protein kinase in the dinoflagellate, Amphidinium operculatum
Cell-cycle studies of the dinoflagellates Gonyaulax polyedra Stein and Gyrodinium uncatenum Hulburt during asexual and sexual reproduction
Garces E, Delgado M, Maso M, Camp J., J. Phycol. 34(5), 1998
PMID: IND44478313
Annotation of unknown yeast ORFs by correlation analysis of microarray data and extensive literature searches.
Pir P, Ulgen KO, Hayes A, Ilsen Onsan Z, Kirdar B, Oliver SG., Yeast 23(7), 2006
PMID: 16710832
Genetic transformation of dinoflagellates (Amphidinium and Symbiodinium): expression of GUS in microalgae using heterologous promoter constructs
C-terminal region of the active domain enhances enzymatic activity in dinoflagellate luciferase
Isolation of algal genes by functional complementation of yeast
An unusual S-adenosylmethionine synthetase gene from dinoflagellate is methylated.
Ho P, Kong KF, Chan YH, Tsang JS, Wong JT., BMC Mol. Biol. 8(), 2007
PMID: 17915037

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

PMID: 20403159
PubMed | Europe PMC

Suchen in

Google Scholar