Ancestry and evolution of a secretory pathway serpin

Kumar A, Ragg H (2008)
BMC Evolutionary Biology 8(1): 250.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
Kumar, Abhishek; Ragg, HermannUniBi
Abstract / Bemerkung
Background:The serpin (serine protease inhibitor) superfamily constitutes a class of functionally highly diverse proteins usually encompassing several dozens of paralogs in mammals. Though phylogenetic classification of vertebrate serpins into six groups based on gene organisation is well established, the evolutionary roots beyond the fish/tetrapod split are unresolved. The aim of this study was to elucidate the phylogenetic relationships of serpins involved in surveying the secretory pathway routes against uncontrolled proteolytic activity. Results: Here, rare genomic characters are used to show that orthologs of neuroserpin, a prominent representative of vertebrate group 3 serpin genes, exist in early diverging deuterostomes and probably also in cnidarians, indicating that the origin of a mammalian serpin can be traced back far in the history of eumetazoans. A C-terminal address code assigning association with secretory pathway organelles is present in all neuroserpin orthologs, suggesting that supervision of cellular export/import routes by antiproteolytic serpins is an ancient trait, though subtle functional and compartmental specialisations have developed during their evolution. The results also suggest that massive changes in the exon-intron organisation of serpin genes have occurred along the lineage leading to vertebrate neuroserpin, in contrast with the immediately adjacent PDCD10 gene that is linked to its neighbour at least since divergence of echinoderms. The intron distribution pattern of closely adjacent and co-regulated genes thus may experience quite different fates during evolution of metazoans. Conclusion: This study demonstrates that the analysis of microsynteny and other rare characters can provide insight into the intricate family history of metazoan serpins. Serpins with the capacity to defend the main cellular export/import routes against uncontrolled endogenous and/or foreign proteolytic activity represent an ancient trait in eukaryotes that has been maintained continuously in metazoans though subtle changes affecting function and subcellular location have evolved. It is shown that the intron distribution pattern of neuroserpin gene orthologs has undergone substantial rearrangements during metazoan evolution.
BMC Evolutionary Biology
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Kumar A, Ragg H. Ancestry and evolution of a secretory pathway serpin. BMC Evolutionary Biology. 2008;8(1): 250.
Kumar, A., & Ragg, H. (2008). Ancestry and evolution of a secretory pathway serpin. BMC Evolutionary Biology, 8(1), 250.
Kumar, Abhishek, and Ragg, Hermann. 2008. “Ancestry and evolution of a secretory pathway serpin”. BMC Evolutionary Biology 8 (1): 250.
Kumar, A., and Ragg, H. (2008). Ancestry and evolution of a secretory pathway serpin. BMC Evolutionary Biology 8:250.
Kumar, A., & Ragg, H., 2008. Ancestry and evolution of a secretory pathway serpin. BMC Evolutionary Biology, 8(1): 250.
A. Kumar and H. Ragg, “Ancestry and evolution of a secretory pathway serpin”, BMC Evolutionary Biology, vol. 8, 2008, : 250.
Kumar, A., Ragg, H.: Ancestry and evolution of a secretory pathway serpin. BMC Evolutionary Biology. 8, : 250 (2008).
Kumar, Abhishek, and Ragg, Hermann. “Ancestry and evolution of a secretory pathway serpin”. BMC Evolutionary Biology 8.1 (2008): 250.
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17 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Ancestry & molecular evolutionary analyses of heat shock protein 47 kDa (HSP47/SERPINH1).
Kumar A, Bhandari A, Sarde SJ, Goswami C., Sci Rep 7(1), 2017
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The aggregation-prone intracellular serpin SRP-2 fails to transit the ER in Caenorhabditis elegans.
Silverman RM, Cummings EE, O'Reilly LP, Miedel MT, Silverman GA, Luke CJ, Perlmutter DH, Pak SC., Genetics 200(1), 2015
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Sequencing, De Novo assembly and annotation of the Colorado Potato Beetle, Leptinotarsa decemlineata, Transcriptome.
Kumar A, Congiu L, Lindström L, Piiroinen S, Vidotto M, Grapputo A., PLoS One 9(1), 2014
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Revising angiotensinogen from phylogenetic and genetic variants perspectives.
Kumar A, Sarde SJ, Bhandari A., Biochem Biophys Res Commun 446(2), 2014
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Genetic variants and evolutionary analyses of heparin cofactor II.
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Kumar A, Bhandari A, Sarde SJ, Goswami C., Biochem Biophys Res Commun 440(4), 2013
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Origin and evolution of spliceosomal introns.
Rogozin IB, Carmel L, Csuros M, Koonin EV., Biol Direct 7(), 2012
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Multiple gains of spliceosomal introns in a superfamily of vertebrate protease inhibitor genes.
Ragg H, Kumar A, Köster K, Bentele C, Wang Y, Frese MA, Prib N, Krüger O., BMC Evol Biol 9(), 2009
PMID: 19698129

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