Gene disruption and gene replacement in Streptomyces via single stranded DNA transformation of integration vectors
Hillemann D, Pühler A, Wohlleben W (1991)
Nucleic Acids Research 19(4): 727-731.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
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Autor*in
Hillemann, Doris;
Pühler, AlfredUniBi 
;
Wohlleben, Wolfgang
;
Wohlleben, WolfgangEinrichtung
Abstract / Bemerkung
For the isolation of single stranded plasmid DNA, various E. coli and E. coli- Streptomyces shuttle plasmids were equipped with the f1 replication origin. The transformation of some representative Streptomyces species with plasmid vectors occurred irrespective of whether single or double stranded DNA was used. In contrast, the transformation of Streptomyces was 10 to 100 times more efficient when an integration vector was in the single stranded form as opposed to the double stranded form. Streptomyces viridochromogenes was transformed by single stranded DNA integration vectors in order to replace the pat by the tsr gene and generate mutants unable to synthesize phosphinothricin - tripeptide (PTT).
Erscheinungsjahr
1991
Zeitschriftentitel
Nucleic Acids Research
Band
19
Ausgabe
4
Seite(n)
727-731
ISSN
0305-1048
eISSN
1362-4962
Page URI
https://pub.uni-bielefeld.de/record/1773962
Zitieren
Hillemann D, Pühler A, Wohlleben W. Gene disruption and gene replacement in Streptomyces via single stranded DNA transformation of integration vectors. Nucleic Acids Research. 1991;19(4):727-731.
Hillemann, D., Pühler, A., & Wohlleben, W. (1991). Gene disruption and gene replacement in Streptomyces via single stranded DNA transformation of integration vectors. Nucleic Acids Research, 19(4), 727-731. https://doi.org/10.1093/nar/19.4.727
Hillemann, Doris, Pühler, Alfred, and Wohlleben, Wolfgang. 1991. “Gene disruption and gene replacement in Streptomyces via single stranded DNA transformation of integration vectors”. Nucleic Acids Research 19 (4): 727-731.
Hillemann, D., Pühler, A., and Wohlleben, W. (1991). Gene disruption and gene replacement in Streptomyces via single stranded DNA transformation of integration vectors. Nucleic Acids Research 19, 727-731.
Hillemann, D., Pühler, A., & Wohlleben, W., 1991. Gene disruption and gene replacement in Streptomyces via single stranded DNA transformation of integration vectors. Nucleic Acids Research, 19(4), p 727-731.
D. Hillemann, A. Pühler, and W. Wohlleben, “Gene disruption and gene replacement in Streptomyces via single stranded DNA transformation of integration vectors”, Nucleic Acids Research, vol. 19, 1991, pp. 727-731.
Hillemann, D., Pühler, A., Wohlleben, W.: Gene disruption and gene replacement in Streptomyces via single stranded DNA transformation of integration vectors. Nucleic Acids Research. 19, 727-731 (1991).
Hillemann, Doris, Pühler, Alfred, and Wohlleben, Wolfgang. “Gene disruption and gene replacement in Streptomyces via single stranded DNA transformation of integration vectors”. Nucleic Acids Research 19.4 (1991): 727-731.
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Norrander J, Kempe T, Messing J., Gene 26(1), 1983
PMID: 6323249
Norrander J, Kempe T, Messing J., Gene 26(1), 1983
PMID: 6323249
Mutational cloning in Streptomyces and the isolation of antibiotic production genes.
Chater KF, Bruton CJ., Gene 26(1), 1983
PMID: 6323253
Chater KF, Bruton CJ., Gene 26(1), 1983
PMID: 6323253
DNA amplification and an unstable arginine gene in Streptomyces lividans 66.
Altenbuchner J, Cullum J., Mol. Gen. Genet. 195(1-2), 1984
PMID: 6092842
Altenbuchner J, Cullum J., Mol. Gen. Genet. 195(1-2), 1984
PMID: 6092842
Factors affecting the isolation of CCC DNA from Streptomyces lividans and Escherichia coli.
Kieser T., Plasmid 12(1), 1984
PMID: 6387733
Kieser T., Plasmid 12(1), 1984
PMID: 6387733
Plasmid vectors for the genetic analysis and manipulation of rhizobia and other gram-negative bacteria.
Simon R, O'Connell M, Labes M, Puhler A., Meth. Enzymol. 118(), 1986
PMID: 3005803
Simon R, O'Connell M, Labes M, Puhler A., Meth. Enzymol. 118(), 1986
PMID: 3005803
Highly transformable mutants of Streptomyces fradiae defective in several restriction systems.
Matsushima P, Cox KL, Baltz RH., Mol. Gen. Genet. 206(3), 1987
PMID: 3473276
Matsushima P, Cox KL, Baltz RH., Mol. Gen. Genet. 206(3), 1987
PMID: 3473276
The Streptomyces ghanaensis low copy plasmid pSG2 and its use for vector construction.
Wohlleben W, Puhler A., Arch. Microbiol. 148(4), 1987
PMID: 3318753
Wohlleben W, Puhler A., Arch. Microbiol. 148(4), 1987
PMID: 3318753
Plasmid and phage vectors for gene cloning and analysis in Streptomyces.
Hopwood DA, Bibb MJ, Chater KF, Kieser T., Meth. Enzymol. 153(), 1987
PMID: 2828840
Hopwood DA, Bibb MJ, Chater KF, Kieser T., Meth. Enzymol. 153(), 1987
PMID: 2828840
Production of single-stranded plasmid DNA.
Vieira J, Messing J., Meth. Enzymol. 153(), 1987
PMID: 3323803
Vieira J, Messing J., Meth. Enzymol. 153(), 1987
PMID: 3323803
Replacement of Streptomyces hygroscopicus genomic segments with in vitro altered DNA sequences.
Anzai H, Kumada Y, Hara O, Murakami T, Itoh R, Takano E, Imai S, Satoh A, Nagaoka K., J. Antibiot. 41(2), 1988
PMID: 3162728
Anzai H, Kumada Y, Hara O, Murakami T, Itoh R, Takano E, Imai S, Satoh A, Nagaoka K., J. Antibiot. 41(2), 1988
PMID: 3162728
Cloning of a phosphinothricin N-acetyltransferase gene from Streptomyces viridochromogenes Tu494 and its expression in Streptomyces lividans and Escherichia coli.
Strauch E, Wohlleben W, Puhler A., Gene 63(1), 1988
PMID: 3290054
Strauch E, Wohlleben W, Puhler A., Gene 63(1), 1988
PMID: 3290054
Development of a plasmid-cloning system for Streptomyces viridochromogenes Tu494.
Strauch E, Wohlleben W, Puhler A., J. Basic Microbiol. 27(8), 1987
PMID: 3453403
Strauch E, Wohlleben W, Puhler A., J. Basic Microbiol. 27(8), 1987
PMID: 3453403
Streptomyces cloning: useful recombinant DNA systems and a summation of cloned genes.
Tomich PK., Antimicrob. Agents Chemother. 32(10), 1988
PMID: 3056235
Tomich PK., Antimicrob. Agents Chemother. 32(10), 1988
PMID: 3056235
DNA cloning in Streptomyces: resistance genes from antibiotic-producing species.
Thompson CJ, Ward JM, Hopwood DA., Nature 286(5772), 1980
PMID: 6250070
Thompson CJ, Ward JM, Hopwood DA., Nature 286(5772), 1980
PMID: 6250070
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