Gene disruption and gene replacement in Streptomyces via single stranded DNA transformation of integration vectors

Hillemann D, Pühler A, Wohlleben W (1991)
Nucleic Acids Research 19(4): 727-731.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
Hillemann, Doris; Pühler, AlfredUniBi ; Wohlleben, Wolfgang
Abstract / Bemerkung
For the isolation of single stranded plasmid DNA, various E. coli and E. coli- Streptomyces shuttle plasmids were equipped with the f1 replication origin. The transformation of some representative Streptomyces species with plasmid vectors occurred irrespective of whether single or double stranded DNA was used. In contrast, the transformation of Streptomyces was 10 to 100 times more efficient when an integration vector was in the single stranded form as opposed to the double stranded form. Streptomyces viridochromogenes was transformed by single stranded DNA integration vectors in order to replace the pat by the tsr gene and generate mutants unable to synthesize phosphinothricin - tripeptide (PTT).
Nucleic Acids Research
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Hillemann D, Pühler A, Wohlleben W. Gene disruption and gene replacement in Streptomyces via single stranded DNA transformation of integration vectors. Nucleic Acids Research. 1991;19(4):727-731.
Hillemann, D., Pühler, A., & Wohlleben, W. (1991). Gene disruption and gene replacement in Streptomyces via single stranded DNA transformation of integration vectors. Nucleic Acids Research, 19(4), 727-731.
Hillemann, D., Pühler, A., and Wohlleben, W. (1991). Gene disruption and gene replacement in Streptomyces via single stranded DNA transformation of integration vectors. Nucleic Acids Research 19, 727-731.
Hillemann, D., Pühler, A., & Wohlleben, W., 1991. Gene disruption and gene replacement in Streptomyces via single stranded DNA transformation of integration vectors. Nucleic Acids Research, 19(4), p 727-731.
D. Hillemann, A. Pühler, and W. Wohlleben, “Gene disruption and gene replacement in Streptomyces via single stranded DNA transformation of integration vectors”, Nucleic Acids Research, vol. 19, 1991, pp. 727-731.
Hillemann, D., Pühler, A., Wohlleben, W.: Gene disruption and gene replacement in Streptomyces via single stranded DNA transformation of integration vectors. Nucleic Acids Research. 19, 727-731 (1991).
Hillemann, Doris, Pühler, Alfred, and Wohlleben, Wolfgang. “Gene disruption and gene replacement in Streptomyces via single stranded DNA transformation of integration vectors”. Nucleic Acids Research 19.4 (1991): 727-731.
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57 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

The complex whiJ locus mediates environmentally sensitive repression of development of Streptomyces coelicolor A3(2).
Aínsa JA, Bird N, Ryding NJ, Findlay KC, Chater KF., Antonie Van Leeuwenhoek 98(2), 2010
PMID: 20405209
Excisable cassettes: new tools for functional analysis of Streptomyces genomes.
Raynal A, Karray F, Tuphile K, Darbon-Rongère E, Pernodet JL., Appl Environ Microbiol 72(7), 2006
PMID: 16820478
GlcP constitutes the major glucose uptake system of Streptomyces coelicolor A3(2).
van Wezel GP, Mahr K, König M, Traag BA, Pimentel-Schmitt EF, Willimek A, Titgemeyer F., Mol Microbiol 55(2), 2005
PMID: 15659175
Agrobacterium-mediated transformation as a tool for functional genomics in fungi.
Michielse CB, Hooykaas PJ, van den Hondel CA, Ram AF., Curr Genet 48(1), 2005
PMID: 15889258
Specialized osmotic stress response systems involve multiple SigB-like sigma factors in Streptomyces coelicolor.
Viollier PH, Kelemen GH, Dale GE, Nguyen KT, Buttner MJ, Thompson CJ., Mol Microbiol 47(3), 2003
PMID: 12535070
Expression and characterization of the type III polyketide synthase 1,3,6,8-tetrahydroxynaphthalene synthase from Streptomyces coelicolor A3(2).
Izumikawa M, Shipley PR, Hopke JN, O'Hare T, Xiang L, Noel JP, Moore BS., J Ind Microbiol Biotechnol 30(8), 2003
PMID: 12905073
The pqrAB operon is responsible for paraquat resistance in Streptomyces coelicolor.
Cho YH, Kim EJ, Chung HJ, Choi JH, Chater KF, Ahn BE, Shin JH, Roe JH., J Bacteriol 185(23), 2003
PMID: 14617639
The biosynthetic gene cluster of the maytansinoid antitumor agent ansamitocin from Actinosynnema pretiosum.
Yu TW, Bai L, Clade D, Hoffmann D, Toelzer S, Trinh KQ, Xu J, Moss SJ, Leistner E, Floss HG., Proc Natl Acad Sci U S A 99(12), 2002
PMID: 12060743
Region-specific transcriptional activity in the genome of Streptomyces coelicolor A3(2).
Gust B, Spatz K, Spychaj A, Redenbach M., Appl Environ Microbiol 67(8), 2001
PMID: 11472936
Unmarked gene deletion mutagenesis of kstD, encoding 3-ketosteroid Delta1-dehydrogenase, in Rhodococcus erythropolis SQ1 using sacB as counter-selectable marker.
van der Geize R, Hessels GI, van Gerwen R, van der Meijden P, Dijkhuizen L., FEMS Microbiol Lett 205(2), 2001
PMID: 11750802
WhiD and WhiB, homologous proteins required for different stages of sporulation in Streptomyces coelicolor A3(2).
Molle V, Palframan WJ, Findlay KC, Buttner MJ., J Bacteriol 182(5), 2000
PMID: 10671449
WhiA, a protein of unknown function conserved among gram-positive bacteria, is essential for sporulation in Streptomyces coelicolor A3(2).
Aínsa JA, Ryding NJ, Hartley N, Findlay KC, Bruton CJ, Chater KF., J Bacteriol 182(19), 2000
PMID: 10986251
Identification of DNA amplifications near the center of the Streptomyces coelicolor M145 chromosome.
Redenbach M, Kleinert E, Stoll A., FEMS Microbiol Lett 191(1), 2000
PMID: 11004409
Alteration of the synthesis of the Clp ATP-dependent protease affects morphological and physiological differentiation in Streptomyces.
de Crécy-Lagard V, Servant-Moisson P, Viala J, Grandvalet C, Mazodier P., Mol Microbiol 32(3), 1999
PMID: 10320574
The Streptomyces coelicolor sporulation-specific sigma WhiG form of RNA polymerase transcribes a gene encoding a ProX-like protein that is dispensable for sporulation.
Tan H, Yang H, Tian Y, Wu W, Whatling CA, Chamberlin LC, Buttner MJ, Nodwell J, Chater KF., Gene 212(1), 1998
PMID: 9661673
Pristinamycin I biosynthesis in Streptomyces pristinaespiralis: molecular characterization of the first two structural peptide synthetase genes.
de Crécy-Lagard V, Blanc V, Gil P, Naudin L, Lorenzon S, Famechon A, Bamas-Jacques N, Crouzet J, Thibaut D., J Bacteriol 179(3), 1997
PMID: 9006024
Characterization of two Streptomyces ambofaciens recA mutants: identification of the RecA protein by immunoblotting.
Aigle B, Holl AC, Angulo JF, Leblond P, Decaris B., FEMS Microbiol Lett 149(2), 1997
PMID: 9141659
Cloning of an avilamycin biosynthetic gene cluster from Streptomyces viridochromogenes Tü57.
Gaisser S, Trefzer A, Stockert S, Kirschning A, Bechthold A., J Bacteriol 179(20), 1997
PMID: 9335272
Engineering antibiotic producers to overcome the limitations of classical strain improvement programs.
Lal R, Khanna R, Kaur H, Khanna M, Dhingra N, Lal S, Gartemann KH, Eichenlaub R, Ghosh PK., Crit Rev Microbiol 22(4), 1996
PMID: 8989512
Analysis of a gene that suppresses the morphological defect of bald mutants of Streptomyces griseus.
McCue LA, Kwak J, Wang J, Kendrick KE., J Bacteriol 178(10), 1996
PMID: 8631675
Efficient sampling of protein sequence space for multiple mutants.
Caren R, Mørkeberg R, Khosla C., Biotechnology (N Y) 12(5), 1994
PMID: 7764711
Effects of disruption of xylanase-encoding genes on the xylanolytic system of Streptomyces lividans.
Arhin FF, Shareck F, Kluepfel D, Morosoli R., J Bacteriol 176(16), 1994
PMID: 8051006
Targeted recombination with single-stranded DNA vectors in mammalian cells.
Fujioka K, Aratani Y, Kusano K, Koyama H., Nucleic Acids Res 21(3), 1993
PMID: 8441653
Accumulation of bldA-specified tRNA is temporally regulated in Streptomyces coelicolor A3(2).
Leskiw BK, Mah R, Lawlor EJ, Chater KF., J Bacteriol 175(7), 1993
PMID: 8458842
Transpositional activity of IS986 in Mycobacterium smegmatis.
Fomukong NG, Dale JW., Gene 130(1), 1993
PMID: 8393827
Analysis of Streptomyces avermitilis genes required for avermectin biosynthesis utilizing a novel integration vector.
MacNeil DJ, Gewain KM, Ruby CL, Dezeny G, Gibbons PH, MacNeil T., Gene 111(1), 1992
PMID: 1547955

22 References

Daten bereitgestellt von Europe PubMed Central.

[Metabolic products of microorganisms. 98. Phosphinothricin and phosphinothricyl-alanyl-analine]
Bayer E, Gugel KH, Hagele K, Hagenmaier H, Jessipow S, Konig WA, Zahner H., Helv. Chim. Acta 55(1), 1972
PMID: 5010035
A rapid boiling method for the preparation of bacterial plasmids.
Holmes DS, Quigley M., Anal. Biochem. 114(1), 1981
PMID: 6269464
pIJ101, a multi-copy broad host-range Streptomyces plasmid: functional analysis and development of DNA cloning vectors.
Kieser T, Hopwood DA, Wright HM, Thompson CJ., Mol. Gen. Genet. 185(2), 1982
PMID: 6283316
DNA amplification and an unstable arginine gene in Streptomyces lividans 66.
Altenbuchner J, Cullum J., Mol. Gen. Genet. 195(1-2), 1984
PMID: 6092842
Plasmid vectors for the genetic analysis and manipulation of rhizobia and other gram-negative bacteria.
Simon R, O'Connell M, Labes M, Puhler A., Meth. Enzymol. 118(), 1986
PMID: 3005803
Highly transformable mutants of Streptomyces fradiae defective in several restriction systems.
Matsushima P, Cox KL, Baltz RH., Mol. Gen. Genet. 206(3), 1987
PMID: 3473276
The Streptomyces ghanaensis low copy plasmid pSG2 and its use for vector construction.
Wohlleben W, Puhler A., Arch. Microbiol. 148(4), 1987
PMID: 3318753
Plasmid and phage vectors for gene cloning and analysis in Streptomyces.
Hopwood DA, Bibb MJ, Chater KF, Kieser T., Meth. Enzymol. 153(), 1987
PMID: 2828840
Production of single-stranded plasmid DNA.
Vieira J, Messing J., Meth. Enzymol. 153(), 1987
PMID: 3323803
Replacement of Streptomyces hygroscopicus genomic segments with in vitro altered DNA sequences.
Anzai H, Kumada Y, Hara O, Murakami T, Itoh R, Takano E, Imai S, Satoh A, Nagaoka K., J. Antibiot. 41(2), 1988
PMID: 3162728
Development of a plasmid-cloning system for Streptomyces viridochromogenes Tu494.
Strauch E, Wohlleben W, Puhler A., J. Basic Microbiol. 27(8), 1987
PMID: 3453403
Streptomyces cloning: useful recombinant DNA systems and a summation of cloned genes.
Tomich PK., Antimicrob. Agents Chemother. 32(10), 1988
PMID: 3056235
DNA cloning in Streptomyces: resistance genes from antibiotic-producing species.
Thompson CJ, Ward JM, Hopwood DA., Nature 286(5772), 1980
PMID: 6250070

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