Tight linkage of ginA and a putative regulatory gene in Rhizobium leguminosarum

Colonna-Romano S, Riccio A, Guida M, Defez R, Lamberti A, Iaccarino M, Arnold W, Priefer UB, Pühler A (1987)
Nucleic Acids Research 15(5): 1951-1964.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Rhizobium leguminosarum, biovar viceae, strain RCC1001 contains two glutamine synthetase activities, GSI and GSII. We report here the identification of glnA, the structural gene for GSI. A 2 kb fragment of DNA was shown to complement the Gln- phenotype of Klebsiella pneumoniae glnA mutant strains. DNA sequence analysis revealed an open reading frame (ORF) of 469 codons specifying a polypeptide of 52,040 daltons. Its deduced amino acid sequence was found to be highly homologous to other glutamine synthetase sequences. This ORF was expressed in Escherichia coli minicells and the corresponding polypeptide reacted with an antiserum raised against GSI. Upstream of glnA we found an ORF of 111 codons (ORF111) preceded by the consensus sequence for an ntrA-dependent promoter. Minicells experiments showed a protein band, with a molecular weight in good agreement with that (10,469) deduced from the nucleotide sequence. On the basis of homology studies we discuss the possibility that the product of ORF111 is equivalent to the P_II protein of E. coli and plays a similar role in regulation of nitrogen metabolism.
Erscheinungsjahr
1987
Zeitschriftentitel
Nucleic Acids Research
Band
15
Ausgabe
5
Seite(n)
1951-1964
ISSN
0305-1048
eISSN
1362-4962
Page URI
https://pub.uni-bielefeld.de/record/1773947

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Colonna-Romano S, Riccio A, Guida M, et al. Tight linkage of ginA and a putative regulatory gene in Rhizobium leguminosarum. Nucleic Acids Research. 1987;15(5):1951-1964.
Colonna-Romano, S., Riccio, A., Guida, M., Defez, R., Lamberti, A., Iaccarino, M., Arnold, W., et al. (1987). Tight linkage of ginA and a putative regulatory gene in Rhizobium leguminosarum. Nucleic Acids Research, 15(5), 1951-1964. doi:10.1093/nar/15.5.1951
Colonna-Romano, S., Riccio, A., Guida, M., Defez, R., Lamberti, A., Iaccarino, M., Arnold, W., Priefer, U. B., and Pühler, A. (1987). Tight linkage of ginA and a putative regulatory gene in Rhizobium leguminosarum. Nucleic Acids Research 15, 1951-1964.
Colonna-Romano, S., et al., 1987. Tight linkage of ginA and a putative regulatory gene in Rhizobium leguminosarum. Nucleic Acids Research, 15(5), p 1951-1964.
S. Colonna-Romano, et al., “Tight linkage of ginA and a putative regulatory gene in Rhizobium leguminosarum”, Nucleic Acids Research, vol. 15, 1987, pp. 1951-1964.
Colonna-Romano, S., Riccio, A., Guida, M., Defez, R., Lamberti, A., Iaccarino, M., Arnold, W., Priefer, U.B., Pühler, A.: Tight linkage of ginA and a putative regulatory gene in Rhizobium leguminosarum. Nucleic Acids Research. 15, 1951-1964 (1987).
Colonna-Romano, S., Riccio, A., Guida, M., Defez, R., Lamberti, A., Iaccarino, M., Arnold, Walter, Priefer, Ursula B., and Pühler, Alfred. “Tight linkage of ginA and a putative regulatory gene in Rhizobium leguminosarum”. Nucleic Acids Research 15.5 (1987): 1951-1964.
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