Organization and partial sequence of a DNA region of the Rhizobium leguminosarum symbiotic plasmid pRL6JI containing the genes fixABC, nifA, nifB and a novel open reading frame

Grönger P, Manian SS, Reiländer H, O´Connell M, Priefer UB, Pühler A (1987)
Nucleic Acids Research 15(1): 31-49.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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DOI
URN
urn:nbn:de:0070-pub-17739401
Autor*in
Grönger, Petra; Manian, Sundaram S.; Reiländer, Helmut; O´Connell, Michael; Priefer, Ursula B.; Pühler, AlfredUniBi
Einrichtung
Fakultät für Biologie
Abstract / Bemerkung
By hybridization and heteroduplex studies the fixABC and nifA genes of the Rhizobium leguminosarum symbiotic plasmid pRL6JI have been identified. DNA sequencing of the region containing nifA showed an open reading frame of 1557 bp encoding a protein of 56,178 D. Based on sequence homology, this ORF was confirmed to correspond to the nifA gene. Comparison of three nifA proteins (Klebsiella pneumoniae, Rhizobium meliloti, Rhizobium leguminosarum) revealed only a weak relationship in their N-terminal regions, whereas the C-terminal parts exhibited strong homology. Sequence analysis also showed that the R. leguminosarum nifA gene is followed by nifB and preceded by fixC with an open reading frame inserted in between. This novel ORF of 294 bp was found to be highly conserved also in R. meliloti. No known promoter and termination signals could be defined on the sequenced R. leguminosarum fragment.
Erscheinungsjahr
1987
Zeitschriftentitel
Nucleic Acids Research
Band
15
Ausgabe
1
Seite(n)
31-49
ISSN
0305-1048
eISSN
1362-4962
Page URI
https://pub.uni-bielefeld.de/record/1773940

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Grönger P, Manian SS, Reiländer H, O´Connell M, Priefer UB, Pühler A. Organization and partial sequence of a DNA region of the Rhizobium leguminosarum symbiotic plasmid pRL6JI containing the genes fixABC, nifA, nifB and a novel open reading frame. Nucleic Acids Research. 1987;15(1):31-49.
Grönger, P., Manian, S. S., Reiländer, H., O´Connell, M., Priefer, U. B., & Pühler, A. (1987). Organization and partial sequence of a DNA region of the Rhizobium leguminosarum symbiotic plasmid pRL6JI containing the genes fixABC, nifA, nifB and a novel open reading frame. Nucleic Acids Research, 15(1), 31-49. https://doi.org/10.1093/nar/15.1.31
Grönger, Petra, Manian, Sundaram S., Reiländer, Helmut, O´Connell, Michael, Priefer, Ursula B., and Pühler, Alfred. 1987. “Organization and partial sequence of a DNA region of the Rhizobium leguminosarum symbiotic plasmid pRL6JI containing the genes fixABC, nifA, nifB and a novel open reading frame”. Nucleic Acids Research 15 (1): 31-49.
Grönger, P., Manian, S. S., Reiländer, H., O´Connell, M., Priefer, U. B., and Pühler, A. (1987). Organization and partial sequence of a DNA region of the Rhizobium leguminosarum symbiotic plasmid pRL6JI containing the genes fixABC, nifA, nifB and a novel open reading frame. Nucleic Acids Research 15, 31-49.
Grönger, P., et al., 1987. Organization and partial sequence of a DNA region of the Rhizobium leguminosarum symbiotic plasmid pRL6JI containing the genes fixABC, nifA, nifB and a novel open reading frame. Nucleic Acids Research, 15(1), p 31-49.
P. Grönger, et al., “Organization and partial sequence of a DNA region of the Rhizobium leguminosarum symbiotic plasmid pRL6JI containing the genes fixABC, nifA, nifB and a novel open reading frame”, Nucleic Acids Research, vol. 15, 1987, pp. 31-49.
Grönger, P., Manian, S.S., Reiländer, H., O´Connell, M., Priefer, U.B., Pühler, A.: Organization and partial sequence of a DNA region of the Rhizobium leguminosarum symbiotic plasmid pRL6JI containing the genes fixABC, nifA, nifB and a novel open reading frame. Nucleic Acids Research. 15, 31-49 (1987).
Grönger, Petra, Manian, Sundaram S., Reiländer, Helmut, O´Connell, Michael, Priefer, Ursula B., and Pühler, Alfred. “Organization and partial sequence of a DNA region of the Rhizobium leguminosarum symbiotic plasmid pRL6JI containing the genes fixABC, nifA, nifB and a novel open reading frame”. Nucleic Acids Research 15.1 (1987): 31-49.
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27 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Expression and functional analysis of the Rhizobium meliloti nifA gene.
Beynon JL, Williams MK, Cannon FC., EMBO J 7(1), 1988
PMID: 16453824
Deduced products of C4-dicarboxylate transport regulatory genes of Rhizobium leguminosarum are homologous to nitrogen regulatory gene products.
Ronson CW, Astwood PM, Nixon BT, Ausubel FM., Nucleic Acids Res 15(19), 1987
PMID: 3671068

37 References

Daten bereitgestellt von Europe PubMed Central.

An efficient method for finding repeats in molecular sequences.
Martinez HM., Nucleic Acids Res. 11(13), 1983
PMID: 6866775
A multiple sequence alignment program.
Sobel E, Martinez HM., Nucleic Acids Res. 14(1), 1986
PMID: 3753772
Sequence and domain relationships of ntrC and nifA from Klebsiella pneumoniae: homologies to other regulatory proteins.
Drummond M, Whitty P, Wootton J., EMBO J. 5(2), 1986
PMID: 3011408
Klebsiella pneumoniae nifA product activates the Rhizobium meliloti nitrogenase promoter.
Sundaresan V, Jones JD, Ow DW, Ausubel FM., Nature 301(5902), 1983
PMID: 6338395
The nif promoters of Klebsiella pneumoniae have a characteristic primary structure.
Beynon J, Cannon M, Buchanan-Wollaston V, Cannon F., Cell 34(2), 1983
PMID: 6311436
Deletion analysis of Rhizobium meliloti symbiotic promoters.
Better M, Ditta G, Helinski DR., EMBO J. 4(10), 1985
PMID: 15929217
Regulation of nitrogen metabolism genes by nifA gene product in Klebsiella pneumoniae.
Ow DW, Ausubel FM., Nature 301(5898), 1983
PMID: 6337346
Mutational analysis of the Klebsiella pneumoniae nitrogenase promoter: sequences essential for positive control by nifA and ntrC (glnG) products.
Ow DW, Xiong Y, Gu Q, Shen SC., J. Bacteriol. 161(3), 1985
PMID: 3882668
Deletion analysis of the Klebsiella pneumoniae nitrogenase promoter: importance of spacing between conserved sequences around positions -12 and -24 for activation by the nifA and ntrC (glnG) products.
Buck M., J. Bacteriol. 166(2), 1986
PMID: 3009407
Activation of the Bradyrhizobium japonicum nifH and nifDK operons is dependent on promoter-upstream DNA sequences.
Alvarez-Morales A, Betancourt-Alvarez M, Kaluza K, Hennecke H., Nucleic Acids Res. 14(10), 1986
PMID: 3086837
DNA sequence of the Rhizobium leguminosarum nodulation genes nodAB and C required for root hair curling.
Rossen L, Johnston AW, Downie JA., Nucleic Acids Res. 12(24), 1984
PMID: 6514582
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
Vieira J, Messing J., Gene 19(3), 1982
PMID: 6295879
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