Direct selection for curing and deletion of Rhizobium plasmids using transposons carrying the Bacillus subtilis sacB gene

Hynes MF, Quandt J, O'Connell MP, Pühler A (1989)
Gene 78(1): 111-120.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
DOI
Autor*in
Hynes, M. F.; Quandt, J.; O'Connell, M. P.; Pühler, AlfredUniBi
Einrichtung
Fakultät für Biologie
Abstract / Bemerkung
We have constructed derivatives of the transposon Tn5 carrying the mob site (oriT) of plasmid RP4, and an nptI-sacB-sacR cassette [Ried and Collmer, Gene 57 (1987) 239-246]. The mob site, in conjunction with the antibiotic-resistance markers carried on the transposons, allows identification of transposon inserts in cryptic plasmids by mobilisation to other strains. The sacB-sacR genes allow direct selection for the loss or curing of plasmids, because only strains which no longer contain an active sacB gene are able to grow on media containing sucrose. We have tested these transposons in four strains of Rhizobium leguminosarum and two strains of Rhizobium meliloti, and have been able to demonstrate curing of several large cryptic plasmids, and generation of large deletions in many other plasmids. This method has enabled us to show that the R. leguminosarum plasmids pRL12JI and pR1eVF39f carry auxotrophic markers, and that the plasmid pR1eVF39c carries genes which affect colony morphology.
Erscheinungsjahr
1989
Zeitschriftentitel
Gene
Band
78
Ausgabe
1
Seite(n)
111-120
ISSN
0378-1119
Page URI
https://pub.uni-bielefeld.de/record/1652943

Zitieren

Hynes MF, Quandt J, O'Connell MP, Pühler A. Direct selection for curing and deletion of Rhizobium plasmids using transposons carrying the Bacillus subtilis sacB gene. Gene. 1989;78(1):111-120.
Hynes, M. F., Quandt, J., O'Connell, M. P., & Pühler, A. (1989). Direct selection for curing and deletion of Rhizobium plasmids using transposons carrying the Bacillus subtilis sacB gene. Gene, 78(1), 111-120. https://doi.org/10.1016/0378-1119(89)90319-3
Hynes, M. F., Quandt, J., O'Connell, M. P., and Pühler, Alfred. 1989. “Direct selection for curing and deletion of Rhizobium plasmids using transposons carrying the Bacillus subtilis sacB gene”. Gene 78 (1): 111-120.
Hynes, M. F., Quandt, J., O'Connell, M. P., and Pühler, A. (1989). Direct selection for curing and deletion of Rhizobium plasmids using transposons carrying the Bacillus subtilis sacB gene. Gene 78, 111-120.
Hynes, M.F., et al., 1989. Direct selection for curing and deletion of Rhizobium plasmids using transposons carrying the Bacillus subtilis sacB gene. Gene, 78(1), p 111-120.
M.F. Hynes, et al., “Direct selection for curing and deletion of Rhizobium plasmids using transposons carrying the Bacillus subtilis sacB gene”, Gene, vol. 78, 1989, pp. 111-120.
Hynes, M.F., Quandt, J., O'Connell, M.P., Pühler, A.: Direct selection for curing and deletion of Rhizobium plasmids using transposons carrying the Bacillus subtilis sacB gene. Gene. 78, 111-120 (1989).
Hynes, M. F., Quandt, J., O'Connell, M. P., and Pühler, Alfred. “Direct selection for curing and deletion of Rhizobium plasmids using transposons carrying the Bacillus subtilis sacB gene”. Gene 78.1 (1989): 111-120.

60 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Effect of point mutations on Herbaspirillum seropedicae NifA activity.
Aquino B, Stefanello AA, Oliveira MA, Pedrosa FO, Souza EM, Monteiro RA, Chubatsu LS., Braz. J. Med. Biol. Res. 48(8), 2015
PMID: 26176311
Taxonomy of rhizobia and agrobacteria from the Rhizobiaceae family in light of genomics.
Ormeno-Orrillo E, Servin-Garciduenas LE, Rogel MA, Gonzalez V, Peralta H, Mora J, Martinez-Romero J, Martinez-Romero E., Syst. Appl. Microbiol. 38(4), 2015
PMID: 25660942
Counter-transcribed RNAs of Rhizobium leguminosarum repABC plasmids exert incompatibility effects only when highly expressed.
Yip CB, Ding H, Hynes MF., Plasmid 78(), 2015
PMID: 25530178
Functional relationships between plasmids and their significance for metabolism and symbiotic performance of Rhizobium leguminosarum bv. trifolii.
Stasiak G, Mazur A, Wielbo J, Marczak M, Zebracki K, Koper P, Skorupska A., J. Appl. Genet. 55(4), 2014
PMID: 24839164
Enhancement of alkaline protease production in Bacillus circulans using plasmid transformation
Sabir JSM, El-Bestawy E., World J. Microbiol. Biotechnol. 25(11), 2009
PMID: IND44268661
Enhancement of nodulation by some arid climate strains of Rhizobium leguminosarum biovar trifolii using protoplast fusion
Sabir JSM, El-Bestawy E., World J. Microbiol. Biotechnol. 25(4), 2009
PMID: IND44174082
Incompatibility behavior of a megaplasmid pMhHN3015c in Mesorhizobium huakuii HN3015
Hu G, Li Y, Zhou J., World J. Microbiol. Biotechnol. 24(8), 2008
PMID: IND44070943
The function of three indigenous plasmids in Mesorhizobium huakuii 2020 and its symbiotic interaction with Sym pJB5JI of Rhizobium leguminosarum.
Yang C, Li Y, Wei L, Cheng G, Zhou J., Sci. China, C, Life Sci. 51(4), 2008
PMID: 18368313
Biological behavior of plasmid in Rhizobium sp. strain S25 from Tephrosia candida.
Zou X, Feng XL, Chen WX, Li FD., Plasmid 40(2), 1998
PMID: 9735317
Effect of drought stress on survival of clover rhizobia W14-2 and its plasmid-cured derivatives in soil.
Moenne-Loccoz Y, Weaver RW., Agric., Ecosyst. Environ., Appl. Soil Ecol. 4(2), 1996
PMID: IND21811847

References

Daten bereitgestellt von Europe PubMed Central.

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®
Quellen

PMID: 2548927
PubMed | Europe PMC

Suchen in

Google Scholar