Increased yield of a lysozyme after self-cloning of the gene in Streptomyces coelicolor "Müller"
Bräu B, Hilgenfeld R, Schlingmann M, Marquardt R, Birr E, Wohlleben W, Aufderheide K, Pühler A (1991)
Applied Microbiology and Biotechnology 34(4): 481-487.
Zeitschriftenaufsatz
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Autor*in
Bräu, B.;
Hilgenfeld, R.;
Schlingmann, M.;
Marquardt, R.;
Birr, E.;
Wohlleben, Wolfgang;
Aufderheide, K.;
Pühler, AlfredUniBi
Einrichtung
Abstract / Bemerkung
Streptomyces coelicolor "Muller" DSM3030 excretes a lysozyme comprising both-beta-1,4-N-acetyl- and beta-1,4-N,6-O-diacetyl muramidase activities. The lysozyme is named Cellosyl. Gene libraries have been established using genomic DNA from the wild-type strain, S. coelicolor DSM3030, and from an overproducing mutant, S. coelicolor HP1, which exhibits about a twofold increase in lysozyme production. The lysozyme-encoding genes (cel) from both strains were detected by oligodeoxynucleotide hybridization. The nucleotide sequence of the cel genes isolated from both strains was shown to be identical. The different levels of lysozyme production could not be correlated with any mutations at the cel gene locus. The cel gene isolated from the wild-type strain could not be expressed in some other species of Streptomyces. However, self-cloning of the cel gene into S. coelicolor DSM3030 and HP1 resulted in a 2.5-fold increase in lysozyme production.
Erscheinungsjahr
1991
Zeitschriftentitel
Applied Microbiology and Biotechnology
Band
34
Ausgabe
4
Seite(n)
481-487
ISSN
0175-7598
Page URI
https://pub.uni-bielefeld.de/record/1650416
Zitieren
Bräu B, Hilgenfeld R, Schlingmann M, et al. Increased yield of a lysozyme after self-cloning of the gene in Streptomyces coelicolor "Müller". Applied Microbiology and Biotechnology. 1991;34(4):481-487.
Bräu, B., Hilgenfeld, R., Schlingmann, M., Marquardt, R., Birr, E., Wohlleben, W., Aufderheide, K., et al. (1991). Increased yield of a lysozyme after self-cloning of the gene in Streptomyces coelicolor "Müller". Applied Microbiology and Biotechnology, 34(4), 481-487.
Bräu, B., Hilgenfeld, R., Schlingmann, M., Marquardt, R., Birr, E., Wohlleben, Wolfgang, Aufderheide, K., and Pühler, Alfred. 1991. “Increased yield of a lysozyme after self-cloning of the gene in Streptomyces coelicolor "Müller"”. Applied Microbiology and Biotechnology 34 (4): 481-487.
Bräu, B., Hilgenfeld, R., Schlingmann, M., Marquardt, R., Birr, E., Wohlleben, W., Aufderheide, K., and Pühler, A. (1991). Increased yield of a lysozyme after self-cloning of the gene in Streptomyces coelicolor "Müller". Applied Microbiology and Biotechnology 34, 481-487.
Bräu, B., et al., 1991. Increased yield of a lysozyme after self-cloning of the gene in Streptomyces coelicolor "Müller". Applied Microbiology and Biotechnology, 34(4), p 481-487.
B. Bräu, et al., “Increased yield of a lysozyme after self-cloning of the gene in Streptomyces coelicolor "Müller"”, Applied Microbiology and Biotechnology, vol. 34, 1991, pp. 481-487.
Bräu, B., Hilgenfeld, R., Schlingmann, M., Marquardt, R., Birr, E., Wohlleben, W., Aufderheide, K., Pühler, A.: Increased yield of a lysozyme after self-cloning of the gene in Streptomyces coelicolor "Müller". Applied Microbiology and Biotechnology. 34, 481-487 (1991).
Bräu, B., Hilgenfeld, R., Schlingmann, M., Marquardt, R., Birr, E., Wohlleben, Wolfgang, Aufderheide, K., and Pühler, Alfred. “Increased yield of a lysozyme after self-cloning of the gene in Streptomyces coelicolor "Müller"”. Applied Microbiology and Biotechnology 34.4 (1991): 481-487.
Daten bereitgestellt von European Bioinformatics Institute (EBI)
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