The Corynebacterium glutamicum aecD gene encodes a C-S lyase with alpha, beta-elimination activity that degrades aminoethylcysteine

ROSSOL I, Pühler A (1992)
J Bacteriol 174(9): 2968-2977.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
DOI
Autor*in
ROSSOL, I; Pühler, AlfredUniBi
Einrichtung
Fakultät für Biologie
Abstract / Bemerkung
S-(beta-Aminoethyl)-cysteine (AEC) resistance was achieved in Corynebacterium glutamicum by cloning a chromosomal 1.5-kb EcoRV-BglII DNA fragment on a multicopy plasmid. DNA sequence analysis of the 1.5-kb DNA fragment revealed an open reading frame (ORF326) which represents the AEC resistance gene, designated aecD. The aecD gene directs the synthesis of a 36-kDa protein which was visualized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The aecD gene is a nonessential gene and mediates AEC resistance only in an amplified state. C. glutamicum strains harboring an amplified aecD gene can utilize AEC as an alternative nitrogen source, indicating that the AEC resistance mechanism is due to AEC degradation. Since the AEC degradation products analyzed by high-pressure liquid chromatography were found to be pyruvate and aminoethanethiol (cysteamine), it was concluded that the aecD gene encodes a C-S lyase with alpha,beta-elimination activity. Besides AEC, the C-S lyase was also able to use cysteine, cystine, and cystathionine as substrates.
Erscheinungsjahr
1992
Zeitschriftentitel
J Bacteriol
Band
174
Ausgabe
9
Seite(n)
2968-2977
ISSN
0021-9193
Page URI
https://pub.uni-bielefeld.de/record/1648334

Zitieren

ROSSOL I, Pühler A. The Corynebacterium glutamicum aecD gene encodes a C-S lyase with alpha, beta-elimination activity that degrades aminoethylcysteine. J Bacteriol. 1992;174(9):2968-2977.
ROSSOL, I., & Pühler, A. (1992). The Corynebacterium glutamicum aecD gene encodes a C-S lyase with alpha, beta-elimination activity that degrades aminoethylcysteine. J Bacteriol, 174(9), 2968-2977. https://doi.org/10.1128/JB.174.9.2968-2977.1992
ROSSOL, I, and Pühler, Alfred. 1992. “The Corynebacterium glutamicum aecD gene encodes a C-S lyase with alpha, beta-elimination activity that degrades aminoethylcysteine”. J Bacteriol 174 (9): 2968-2977.
ROSSOL, I., and Pühler, A. (1992). The Corynebacterium glutamicum aecD gene encodes a C-S lyase with alpha, beta-elimination activity that degrades aminoethylcysteine. J Bacteriol 174, 2968-2977.
ROSSOL, I., & Pühler, A., 1992. The Corynebacterium glutamicum aecD gene encodes a C-S lyase with alpha, beta-elimination activity that degrades aminoethylcysteine. J Bacteriol, 174(9), p 2968-2977.
I. ROSSOL and A. Pühler, “The Corynebacterium glutamicum aecD gene encodes a C-S lyase with alpha, beta-elimination activity that degrades aminoethylcysteine”, J Bacteriol, vol. 174, 1992, pp. 2968-2977.
ROSSOL, I., Pühler, A.: The Corynebacterium glutamicum aecD gene encodes a C-S lyase with alpha, beta-elimination activity that degrades aminoethylcysteine. J Bacteriol. 174, 2968-2977 (1992).
ROSSOL, I, and Pühler, Alfred. “The Corynebacterium glutamicum aecD gene encodes a C-S lyase with alpha, beta-elimination activity that degrades aminoethylcysteine”. J Bacteriol 174.9 (1992): 2968-2977.

21 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

The cmaR gene of Corynebacterium ammoniagenes performs a novel regulatory role in the metabolism of sulfur-containing amino acids.
Lee SM, Hwang BJ, Kim Y, Lee HS., Microbiology (Reading, Engl.) 155(Pt 6), 2009
PMID: 19383689
Characteristics of methionine production by an engineered Corynebacterium glutamicum strain.
Park SD, Lee JY, Sim SY, Kim Y, Lee HS., Metab. Eng. 9(4), 2007
PMID: 17604670
Family 4 glycosidases carry out efficient hydrolysis of thioglycosides by an alpha,beta-elimination mechanism.
Yip VL, Withers SG., Angew. Chem. Int. Ed. Engl. 45(37), 2006
PMID: 16917793
Metabolic pathways and biotechnological production of L-cysteine.
Wada M, Takagi H., Appl. Microbiol. Biotechnol. 73(1), 2006
PMID: 17021879
The transcriptional regulator SsuR activates expression of the Corynebacterium glutamicum sulphonate utilization genes in the absence of sulphate.
Koch DJ, Ruckert C, Albersmeier A, Huser AT, Tauch A, Puhler A, Kalinowski J., Mol. Microbiol. 58(2), 2005
PMID: 16194234
Functional analysis of all aminotransferase proteins inferred from the genome sequence of Corynebacterium glutamicum.
Marienhagen J, Kennerknecht N, Sahm H, Eggeling L., J. Bacteriol. 187(22), 2005
PMID: 16267288
The McbR repressor modulated by the effector substance S-adenosylhomocysteine controls directly the transcription of a regulon involved in sulphur metabolism of Corynebacterium glutamicum ATCC 13032.
Rey DA, Nentwich SS, Koch DJ, Ruckert C, Puhler A, Tauch A, Kalinowski J., Mol. Microbiol. 56(4), 2005
PMID: 15853877
Cometabolism of a nongrowth substrate: L-serine utilization by Corynebacterium glutamicum.
Netzer R, Peters-Wendisch P, Eggeling L, Sahm H., Appl. Environ. Microbiol. 70(12), 2004
PMID: 15574911
Genome-based analysis of biosynthetic aminotransferase genes of Corynebacterium glutamicum.
McHardy AC, Tauch A, Ruckert C, Puhler A, Kalinowski J., J. Biotechnol. 104(1-3), 2003
PMID: 12948641
The putative transcriptional repressor McbR, member of the TetR-family, is involved in the regulation of the metabolic network directing the synthesis of sulfur containing amino acids in Corynebacterium glutamicum.
Rey DA, Puhler A, Kalinowski J., J. Biotechnol. 103(1), 2003
PMID: 12770504
Genome-wide analysis of the L-methionine biosynthetic pathway in Corynebacterium glutamicum by targeted gene deletion and homologous complementation.
Ruckert C, Puhler A, Kalinowski J., J. Biotechnol. 104(1-3), 2003
PMID: 12948640
The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins.
Kalinowski J, Bathe B, Bartels D, Bischoff N, Bott M, Burkovski A, Dusch N, Eggeling L, Eikmanns BJ, Gaigalat L, Goesmann A, Hartmann M, Huthmacher K, Kramer R, Linke B, McHardy AC, Meyer F, Mockel B, Pfefferle W, Puhler A, Rey DA, Ruckert C, Rupp O, Sahm H, Wendisch VF, Wiegrabe I, Tauch A., J. Biotechnol. 104(1-3), 2003
PMID: 12948626
Purification, characterization and identification of cysteine desulfhydrase of Corynebacterium glutamicum, and its relationship to cysteine production.
Wada M, Awano N, Haisa K, Takagi H, Nakamori S., FEMS Microbiol. Lett. 217(1), 2002
PMID: 12445652
X-ray structure of MalY from Escherichia coli: a pyridoxal 5'-phosphate-dependent enzyme acting as a modulator in mal gene expression.
Clausen T, Schlegel A, Peist R, Schneider E, Steegborn C, Chang YS, Haase A, Bourenkov GP, Bartunik HD, Boos W., EMBO J. 19(5), 2000
PMID: 10698925
Hemoxidation and binding of the 46-kDa cystalysin of Treponema denticola leads to a cysteine-dependent hemolysis of human erythrocytes.
Chu L, Ebersole JL, Holt SC., Oral Microbiol. Immunol. 14(5), 1999
PMID: 10551156
Cystalysin, a 46-kilodalton cysteine desulfhydrase from Treponema denticola, with hemolytic and hemoxidative activities.
Chu L, Ebersole JL, Kurzban GP, Holt SC., Infect. Immun. 65(8), 1997
PMID: 9234780
A physical and genetic map of the Corynebacterium glutamicum ATCC 13032 chromosome.
Bathe B, Kalinowski J, Puhler A., Mol. Gen. Genet. 252(3), 1996
PMID: 8842145
Evolutionary recruitment of biochemically specialized subdivisions of Family I within the protein superfamily of aminotransferases.
Jensen RA, Gu W., J. Bacteriol. 178(8), 1996
PMID: 8636014
Recent advances in the physiology and genetics of amino acid-producing bacteria.
Jetten MS, Sinskey AJ., Crit. Rev. Biotechnol. 15(1), 1995
PMID: 7736600
The 46-kilodalton-hemolysin gene from Treponema denticola encodes a novel hemolysin homologous to aminotransferases.
Chu L, Burgum A, Kolodrubetz D, Holt SC., Infect. Immun. 63(11), 1995
PMID: 7591084
MalY of Escherichia coli is an enzyme with the activity of a beta C-S lyase (cystathionase).
Zdych E, Peist R, Reidl J, Boos W., J. Bacteriol. 177(17), 1995
PMID: 7665481

2 References

Daten bereitgestellt von Europe PubMed Central.

DNA amplification and an unstable arginine gene in Streptomyces lividans 66.
Altenbuchner J, Cullum J., Mol. Gen. Genet. 195(1-2), 1984
PMID: 6092842
A family of high-copy-number plasmid vectors with single end-label sites for rapid nucleotide sequencing.
Arnold W, Puhler A., Gene 70(1), 1988
PMID: 2907323
Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®
Quellen

PMID: 1569026
PubMed | Europe PMC

Suchen in

Google Scholar