Analysis of the Rhizobium meliloti exoH/exoK/exoL fragment: ExoK shows homology to excreted endo-beta-1,3-1,4-glucanases and ExoH resembles membrane proteins

BECKER A, KLEICKMANN A, Arnold W, Pühler A (1993)
Mol Gen Genet 238(1-2): 145-154.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
BECKER, A; KLEICKMANN, A; Arnold, WalterUniBi; Pühler, AlfredUniBi
Abstract / Bemerkung
Nucleotide sequencing of a 4.15 kb DNA fragment from megaplasmid 2 of Rhizobium meliloti 2011 revealed the location of the genes exoH, exoK and exoL. The putative proteins encoded by these genes have molecular weights of 41, 30, and 44 kDa, respectively. The hydrophobicity profile of the ExoH amino acid sequence resembles that of transmembrane proteins. The predicted exoL gene product does not contain hydrophobic regions, indicating a cytoplasmic localization. The exoK gene product is characterized by a putative signal peptide and exhibits significant homology to endo-beta-1,3-1,4-glucanases of bacilli and Clostridium thermocellum. R. meliloti exoK mutants induced pink nodules and synthesized a reduced amount of exopolysaccharide (EPS). Colonies of this mutant showed a delay in the appearance of the Calcofluor white fluorescence. In addition, the formation of the characteristic halo was strongly delayed. R. meliloti exoL and exoH mutants induced pseudonodules. The exoH, but not the exoL mutant, synthesized an EPS that could be precipitated by cetyl pyridinium chloride (CPC) and also by ethanol. Plasmid integration mutagenesis revealed promoter regions preceding exoH, exoK and exoL.
Stichworte
ENDO-BETA-1; ACIDIC EXOPOLYSACCHARIDE (EPS); RHIZOBIUM-MELILOTI; ALFALFA NODULE; NUCLEOTIDE SEQUENCE; INFECTION; 3-1; 4-GLUCANASE
Erscheinungsjahr
1993
Zeitschriftentitel
Mol Gen Genet
Band
238
Ausgabe
1-2
Seite(n)
145-154
ISSN
0026-8925
Page URI
https://pub.uni-bielefeld.de/record/1645781

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BECKER A, KLEICKMANN A, Arnold W, Pühler A. Analysis of the Rhizobium meliloti exoH/exoK/exoL fragment: ExoK shows homology to excreted endo-beta-1,3-1,4-glucanases and ExoH resembles membrane proteins. Mol Gen Genet. 1993;238(1-2):145-154.
BECKER, A., KLEICKMANN, A., Arnold, W., & Pühler, A. (1993). Analysis of the Rhizobium meliloti exoH/exoK/exoL fragment: ExoK shows homology to excreted endo-beta-1,3-1,4-glucanases and ExoH resembles membrane proteins. Mol Gen Genet, 238(1-2), 145-154. https://doi.org/10.1007/BF00279541
BECKER, A, KLEICKMANN, A, Arnold, Walter, and Pühler, Alfred. 1993. “Analysis of the Rhizobium meliloti exoH/exoK/exoL fragment: ExoK shows homology to excreted endo-beta-1,3-1,4-glucanases and ExoH resembles membrane proteins”. Mol Gen Genet 238 (1-2): 145-154.
BECKER, A., KLEICKMANN, A., Arnold, W., and Pühler, A. (1993). Analysis of the Rhizobium meliloti exoH/exoK/exoL fragment: ExoK shows homology to excreted endo-beta-1,3-1,4-glucanases and ExoH resembles membrane proteins. Mol Gen Genet 238, 145-154.
BECKER, A., et al., 1993. Analysis of the Rhizobium meliloti exoH/exoK/exoL fragment: ExoK shows homology to excreted endo-beta-1,3-1,4-glucanases and ExoH resembles membrane proteins. Mol Gen Genet, 238(1-2), p 145-154.
A. BECKER, et al., “Analysis of the Rhizobium meliloti exoH/exoK/exoL fragment: ExoK shows homology to excreted endo-beta-1,3-1,4-glucanases and ExoH resembles membrane proteins”, Mol Gen Genet, vol. 238, 1993, pp. 145-154.
BECKER, A., KLEICKMANN, A., Arnold, W., Pühler, A.: Analysis of the Rhizobium meliloti exoH/exoK/exoL fragment: ExoK shows homology to excreted endo-beta-1,3-1,4-glucanases and ExoH resembles membrane proteins. Mol Gen Genet. 238, 145-154 (1993).
BECKER, A, KLEICKMANN, A, Arnold, Walter, and Pühler, Alfred. “Analysis of the Rhizobium meliloti exoH/exoK/exoL fragment: ExoK shows homology to excreted endo-beta-1,3-1,4-glucanases and ExoH resembles membrane proteins”. Mol Gen Genet 238.1-2 (1993): 145-154.

31 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Synthesis of Rhizobial Exopolysaccharides and Their Importance for Symbiosis with Legume Plants.
Marczak M, Mazur A, Koper P, Żebracki K, Skorupska A., Genes (Basel) 8(12), 2017
PMID: 29194398
Bacterial exopolysaccharides: biosynthesis pathways and engineering strategies.
Schmid J, Sieber V, Rehm B., Front Microbiol 6(), 2015
PMID: 26074894
Giant virus Megavirus chilensis encodes the biosynthetic pathway for uncommon acetamido sugars.
Piacente F, De Castro C, Jeudy S, Molinaro A, Salis A, Damonte G, Bernardi C, Abergel C, Tonetti MG., J Biol Chem 289(35), 2014
PMID: 25035429
The ExpR/Sin quorum-sensing system controls succinoglycan production in Sinorhizobium meliloti.
Glenn SA, Gurich N, Feeney MA, González JE., J Bacteriol 189(19), 2007
PMID: 17644606
Rhizobial exopolysaccharides: genetic control and symbiotic functions.
Skorupska A, Janczarek M, Marczak M, Mazur A, Król J., Microb Cell Fact 5(), 2006
PMID: 16483356
CbrA is a stationary-phase regulator of cell surface physiology and legume symbiosis in Sinorhizobium meliloti.
Gibson KE, Campbell GR, Lloret J, Walker GC., J Bacteriol 188(12), 2006
PMID: 16740957
Environmental regulation of exopolysaccharide production in Sinorhizobium meliloti.
Mendrygal KE, González JE., J Bacteriol 182(3), 2000
PMID: 10633091
The mdoC gene of Escherichia coli encodes a membrane protein that is required for succinylation of osmoregulated periplasmic glucans.
Lacroix JM, Lanfroy E, Cogez V, Lequette Y, Bohin A, Bohin JP., J Bacteriol 181(12), 1999
PMID: 10368134
The 32-kilobase exp gene cluster of Rhizobium meliloti directing the biosynthesis of galactoglucan: genetic organization and properties of the encoded gene products.
Becker A, Rüberg S, Küster H, Roxlau AA, Keller M, Ivashina T, Cheng HP, Walker GC, Pühler A., J Bacteriol 179(4), 1997
PMID: 9023225
Rhizobium extracellular structures in the symbiosis.
Coronado C, Sánchez-Andújar B, Palomares AJ., World J Microbiol Biotechnol 12(2), 1996
PMID: 24415160
Low molecular weight EPS II of Rhizobium meliloti allows nodule invasion in Medicago sativa.
González JE, Reuhs BL, Walker GC., Proc Natl Acad Sci U S A 93(16), 1996
PMID: 8710923
Rhizobium meliloti exopolysaccharides: synthesis and symbiotic function.
González JE, York GM, Walker GC., Gene 179(1), 1996
PMID: 8955640
Effect of Phosphate Limitation on Synthesis of Periplasmic Cyclic (beta)-(1,2)-Glucans.
Breedveld MW, Benesi AJ, Marco ML, Miller KJ., Appl Environ Microbiol 61(3), 1995
PMID: 16534955
A new gene required for cellulose production and a gene encoding cellulolytic activity in Acetobacter xylinum are colocalized with the bcs operon.
Standal R, Iversen TG, Coucheron DH, Fjaervik E, Blatny JM, Valla S., J Bacteriol 176(3), 1994
PMID: 8300521
Cloning and sequencing of a Rhodothermus marinus gene, bglA, coding for a thermostable beta-glucanase and its expression in Escherichia coli.
Spilliaert R, Hreggvidsson GO, Kristjansson JK, Eggertsson G, Palsdottir A., Eur J Biochem 224(3), 1994
PMID: 7925416
Family of glycosyl transferases needed for the synthesis of succinoglycan by Rhizobium meliloti.
Glucksmann MA, Reuber TL, Walker GC., J Bacteriol 175(21), 1993
PMID: 8226645

39 References

Daten bereitgestellt von Europe PubMed Central.

The -24/-12 promoter comes of age.
Thony B, Hennecke H., FEMS Microbiol. Rev. 5(4), 1989
PMID: 2517036
Path of the polypeptide in bacteriorhodopsin.
Engelman DM, Henderson R, McLachlan AD, Wallace BA., Proc. Natl. Acad. Sci. U.S.A. 77(4), 1980
PMID: 6929535
Exopolysaccharide-deficient mutants of Rhizobium meliloti that form ineffective nodules.
Leigh JA, Signer ER, Walker GC., Proc. Natl. Acad. Sci. U.S.A. 82(18), 1985
PMID: 3862129
Structure of the beta-1,3-1,4-glucanase gene of Bacillus macerans: homologies to other beta-glucanases.
Borriss R, Buettner K, Maentsaelae P., Mol. Gen. Genet. 222(2-3), 1990
PMID: 2274030
Conservation of extended promoter regions of nodulation genes in Rhizobium.
Rostas K, Kondorosi E, Horvath B, Simoncsits A, Kondorosi A., Proc. Natl. Acad. Sci. U.S.A. 83(6), 1986
PMID: 16593668
A new method for predicting signal sequence cleavage sites.
von Heijne G., Nucleic Acids Res. 14(11), 1986
PMID: 3714490
Genetic analysis of the membrane insertion and topology of MalF, a cytoplasmic membrane protein of Escherichia coli.
Froshauer S, Green GN, Boyd D, McGovern K, Beckwith J., J. Mol. Biol. 200(3), 1988
PMID: 3294421
In vitro packaging of lambda and cosmid DNA.
Hohn B., Meth. Enzymol. 68(), 1979
PMID: 161604
Exogenous suppression of the symbiotic deficiencies of Rhizobium meliloti exo mutants.
Urzainqui A, Walker GC., J. Bacteriol. 174(10), 1992
PMID: 1577707
Chromatin reconstitution on small DNA rings. II. DNA supercoiling on the nucleosome.
Zivanovic Y, Goulet I, Revet B, Le Bret M, Prunell A., J. Mol. Biol. 200(2), 1988
PMID: 2836595
The current status and portability of our sequence handling software.
Staden R., Nucleic Acids Res. 14(1), 1986
PMID: 3511446
A simple method for subcloning DNA fragments from gel slices.
Heery DM, Gannon F, Powell R., Trends Genet. 6(6), 1990
PMID: 2371749
Lipid-bound saccharides in Rhizobium meliloti.
Tolmasky ME, Staneloni RJ, Leloir LF., J. Biol. Chem. 257(12), 1982
PMID: 7085601
Rapid and sensitive protein similarity searches.
Lipman DJ, Pearson WR., Science 227(4693), 1985
PMID: 2983426
A simplified protocol for fast plasmid DNA sequencing.
Zimmermann J, Voss H, Schwager C, Stegemann J, Erfle H, Stucky K, Kristensen T, Ansorge W., Nucleic Acids Res. 18(4), 1990
PMID: 2315028
Analysis of membrane and surface protein sequences with the hydrophobic moment plot.
Eisenberg D, Schwarz E, Komaromy M, Wall R., J. Mol. Biol. 179(1), 1984
PMID: 6502707
Codon preference and its use in identifying protein coding regions in long DNA sequences.
Staden R, McLachlan AD., Nucleic Acids Res. 10(1), 1982
PMID: 7063399
R factor transfer in Rhizobium leguminosarum.
Beringer JE., J. Gen. Microbiol. 84(1), 1974
PMID: 4612098
Compilation and analysis of Escherichia coli promoter DNA sequences.
Hawley DK, McClure WR., Nucleic Acids Res. 11(8), 1983
PMID: 6344016
Transcription regulation of peroxisomal fatty acyl-CoA oxidase and enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase in rat liver by peroxisome proliferators.
Reddy JK, Goel SK, Nemali MR, Carrino JJ, Laffler TG, Reddy MK, Sperbeck SJ, Osumi T, Hashimoto T, Lalwani ND., Proc. Natl. Acad. Sci. U.S.A. 83(6), 1986
PMID: 3456610
Symbiotic mutants of Rhizobium meliloti that uncouple plant from bacterial differentiation.
Finan TM, Hirsch AM, Leigh JA, Johansen E, Kuldau GA, Deegan S, Walker GC, Signer ER., Cell 40(4), 1985
PMID: 2985267
The DNA sequence of the gene and genetic control sites for the excreted B. subtilis enzyme beta-glucanase.
Murphy N, McConnell DJ, Cantwell BA., Nucleic Acids Res. 12(13), 1984
PMID: 6087283
Prediction of the secondary structure of proteins from their amino acid sequence.
Chou PY, Fasman GD., Adv. Enzymol. Relat. Areas Mol. Biol. 47(), 1978
PMID: 364941
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