Analysis of the Rhizobium meliloti exoH/exoK/exoL fragment: ExoK shows homology to excreted endo-beta-1,3-1,4-glucanases and ExoH resembles membrane proteins

BECKER A, KLEICKMANN A, Arnold W, Pühler A (1993)
Mol Gen Genet 238(1-2): 145-154.

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DOI
Autor*in
BECKER, A; KLEICKMANN, A; Arnold, WalterUniBi; Pühler, AlfredUniBi
Einrichtung
Fakultät für Biologie
Abstract / Bemerkung
Nucleotide sequencing of a 4.15 kb DNA fragment from megaplasmid 2 of Rhizobium meliloti 2011 revealed the location of the genes exoH, exoK and exoL. The putative proteins encoded by these genes have molecular weights of 41, 30, and 44 kDa, respectively. The hydrophobicity profile of the ExoH amino acid sequence resembles that of transmembrane proteins. The predicted exoL gene product does not contain hydrophobic regions, indicating a cytoplasmic localization. The exoK gene product is characterized by a putative signal peptide and exhibits significant homology to endo-beta-1,3-1,4-glucanases of bacilli and Clostridium thermocellum. R. meliloti exoK mutants induced pink nodules and synthesized a reduced amount of exopolysaccharide (EPS). Colonies of this mutant showed a delay in the appearance of the Calcofluor white fluorescence. In addition, the formation of the characteristic halo was strongly delayed. R. meliloti exoL and exoH mutants induced pseudonodules. The exoH, but not the exoL mutant, synthesized an EPS that could be precipitated by cetyl pyridinium chloride (CPC) and also by ethanol. Plasmid integration mutagenesis revealed promoter regions preceding exoH, exoK and exoL.
Stichworte
ENDO-BETA-1; ACIDIC EXOPOLYSACCHARIDE (EPS); RHIZOBIUM-MELILOTI; ALFALFA NODULE; NUCLEOTIDE SEQUENCE; INFECTION; 3-1; 4-GLUCANASE
Erscheinungsjahr
1993
Zeitschriftentitel
Mol Gen Genet
Band
238
Ausgabe
1-2
Seite(n)
145-154
ISSN
0026-8925
Page URI
https://pub.uni-bielefeld.de/record/1645781

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BECKER A, KLEICKMANN A, Arnold W, Pühler A. Analysis of the Rhizobium meliloti exoH/exoK/exoL fragment: ExoK shows homology to excreted endo-beta-1,3-1,4-glucanases and ExoH resembles membrane proteins. Mol Gen Genet. 1993;238(1-2):145-154.
BECKER, A., KLEICKMANN, A., Arnold, W., & Pühler, A. (1993). Analysis of the Rhizobium meliloti exoH/exoK/exoL fragment: ExoK shows homology to excreted endo-beta-1,3-1,4-glucanases and ExoH resembles membrane proteins. Mol Gen Genet, 238(1-2), 145-154. https://doi.org/10.1007/BF00279541
BECKER, A, KLEICKMANN, A, Arnold, Walter, and Pühler, Alfred. 1993. “Analysis of the Rhizobium meliloti exoH/exoK/exoL fragment: ExoK shows homology to excreted endo-beta-1,3-1,4-glucanases and ExoH resembles membrane proteins”. Mol Gen Genet 238 (1-2): 145-154.
BECKER, A., KLEICKMANN, A., Arnold, W., and Pühler, A. (1993). Analysis of the Rhizobium meliloti exoH/exoK/exoL fragment: ExoK shows homology to excreted endo-beta-1,3-1,4-glucanases and ExoH resembles membrane proteins. Mol Gen Genet 238, 145-154.
BECKER, A., et al., 1993. Analysis of the Rhizobium meliloti exoH/exoK/exoL fragment: ExoK shows homology to excreted endo-beta-1,3-1,4-glucanases and ExoH resembles membrane proteins. Mol Gen Genet, 238(1-2), p 145-154.
A. BECKER, et al., “Analysis of the Rhizobium meliloti exoH/exoK/exoL fragment: ExoK shows homology to excreted endo-beta-1,3-1,4-glucanases and ExoH resembles membrane proteins”, Mol Gen Genet, vol. 238, 1993, pp. 145-154.
BECKER, A., KLEICKMANN, A., Arnold, W., Pühler, A.: Analysis of the Rhizobium meliloti exoH/exoK/exoL fragment: ExoK shows homology to excreted endo-beta-1,3-1,4-glucanases and ExoH resembles membrane proteins. Mol Gen Genet. 238, 145-154 (1993).
BECKER, A, KLEICKMANN, A, Arnold, Walter, and Pühler, Alfred. “Analysis of the Rhizobium meliloti exoH/exoK/exoL fragment: ExoK shows homology to excreted endo-beta-1,3-1,4-glucanases and ExoH resembles membrane proteins”. Mol Gen Genet 238.1-2 (1993): 145-154.

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