THE MEMBRANE TOPOLOGY OF THE RHIZOBIUM-MELILOTI C4-DICARBOXYLATE PERMEASE (DCTA) AS DERIVED FROM PROTEIN FUSIONS WITH ESCHERICHIA-COLI K12 ALKALINE-PHOSPHATASE (PHOA) AND BETA-GALACTOSIDASE (LACZ)

Jording D, Pühler A (1993)
MOLECULAR & GENERAL GENETICS 241-241(1-2): 106-114.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
The Rhizobium meliloti dctA gene encodes the C4-dicarboxylate permease which mediates uptake of C4-dicarboxylates, both in free-living and symbiotic cells. Based on the hydrophobicity of the DctA protein, 12 putative membrane spanning regions were predicted. The membrane topology was further analysed by isolating in vivo fusions of DctA to Escherichia coli alkaline phosphatase (PhoA) and E. coli beta-galactosidase (LacZ). Of 10 different fusions 7 indicated a periplasmic and 3 a cytoplasmic location of the corresponding region of the DctA protein. From these data a two-dimensional model of DctA was constructed which comprised twelve transmembrane alpha-helices with the amino-terminus and the carboxy-terminus located in the cytoplasm. In addition, four conserved amino acid motifs present in many eukaryotic and prokaryotic transport proteins were observed.
Stichworte
C4-DICARBOXYLATE TRANSPORT SYSTEM; PROTEIN; TOPOLOGY; MONITOR PROTEIN FUSIONS; RHIZOBIUM-MELILOTI; CONSERVED MOTIFS
Erscheinungsjahr
1993
Zeitschriftentitel
MOLECULAR & GENERAL GENETICS
Band
241-241
Ausgabe
1-2
Seite(n)
106-114
ISSN
0026-8925
eISSN
1617-4623
Page URI
https://pub.uni-bielefeld.de/record/1645118

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Jording D, Pühler A. THE MEMBRANE TOPOLOGY OF THE RHIZOBIUM-MELILOTI C4-DICARBOXYLATE PERMEASE (DCTA) AS DERIVED FROM PROTEIN FUSIONS WITH ESCHERICHIA-COLI K12 ALKALINE-PHOSPHATASE (PHOA) AND BETA-GALACTOSIDASE (LACZ). MOLECULAR & GENERAL GENETICS. 1993;241-241(1-2):106-114.
Jording, D., & Pühler, A. (1993). THE MEMBRANE TOPOLOGY OF THE RHIZOBIUM-MELILOTI C4-DICARBOXYLATE PERMEASE (DCTA) AS DERIVED FROM PROTEIN FUSIONS WITH ESCHERICHIA-COLI K12 ALKALINE-PHOSPHATASE (PHOA) AND BETA-GALACTOSIDASE (LACZ). MOLECULAR & GENERAL GENETICS, 241-241(1-2), 106-114. https://doi.org/10.1007/BF00280207
Jording, D., and Pühler, A. (1993). THE MEMBRANE TOPOLOGY OF THE RHIZOBIUM-MELILOTI C4-DICARBOXYLATE PERMEASE (DCTA) AS DERIVED FROM PROTEIN FUSIONS WITH ESCHERICHIA-COLI K12 ALKALINE-PHOSPHATASE (PHOA) AND BETA-GALACTOSIDASE (LACZ). MOLECULAR & GENERAL GENETICS 241-241, 106-114.
Jording, D., & Pühler, A., 1993. THE MEMBRANE TOPOLOGY OF THE RHIZOBIUM-MELILOTI C4-DICARBOXYLATE PERMEASE (DCTA) AS DERIVED FROM PROTEIN FUSIONS WITH ESCHERICHIA-COLI K12 ALKALINE-PHOSPHATASE (PHOA) AND BETA-GALACTOSIDASE (LACZ). MOLECULAR & GENERAL GENETICS, 241-241(1-2), p 106-114.
D. Jording and A. Pühler, “THE MEMBRANE TOPOLOGY OF THE RHIZOBIUM-MELILOTI C4-DICARBOXYLATE PERMEASE (DCTA) AS DERIVED FROM PROTEIN FUSIONS WITH ESCHERICHIA-COLI K12 ALKALINE-PHOSPHATASE (PHOA) AND BETA-GALACTOSIDASE (LACZ)”, MOLECULAR & GENERAL GENETICS, vol. 241-241, 1993, pp. 106-114.
Jording, D., Pühler, A.: THE MEMBRANE TOPOLOGY OF THE RHIZOBIUM-MELILOTI C4-DICARBOXYLATE PERMEASE (DCTA) AS DERIVED FROM PROTEIN FUSIONS WITH ESCHERICHIA-COLI K12 ALKALINE-PHOSPHATASE (PHOA) AND BETA-GALACTOSIDASE (LACZ). MOLECULAR & GENERAL GENETICS. 241-241, 106-114 (1993).
Jording, Doris, and Pühler, Alfred. “THE MEMBRANE TOPOLOGY OF THE RHIZOBIUM-MELILOTI C4-DICARBOXYLATE PERMEASE (DCTA) AS DERIVED FROM PROTEIN FUSIONS WITH ESCHERICHIA-COLI K12 ALKALINE-PHOSPHATASE (PHOA) AND BETA-GALACTOSIDASE (LACZ)”. MOLECULAR & GENERAL GENETICS 241-241.1-2 (1993): 106-114.

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