New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions

Becker A, Schmidt M, Jäger W, Pühler A (1995)
GENE 162(1): 37-39.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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DOI
Autor*in
Becker, A; Schmidt, M; Jäger, W; Pühler, AlfredUniBi
Einrichtung
Fakultät für Biologie
Abstract / Bemerkung
A set of antibiotic-resistance and promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions was constructed. The cassettes contain the aacC1 gene of transposon Tn1696 conferring resistance to gentamicin in a large variety of Gram(-) and Gram(+) bacteria. In addition to the antibiotic-resistance gene, a promoterless Escherichia coli lacZ gene was included in the cassettes, allowing the determination of the transcriptional activity at the insertion site. The cassettes can be excised from a plasmid mediating ampicillin resistance by many commonly used restriction enzymes. The new constructs have been successfully used for mutagenesis and studies of gene transcription in Rhizobium meliloti.
Stichworte
ACETYLTRANSFERASE-3-I(AAC(3)-I); BETA-GALACTOSIDASE; TRANSCRIPTION; MONITORING; RHIZOBIUM MELILOTI; RECOMBINANT DNA; REPORTER GENE CASSETTE; GENTAMICIN; EXO GENES; SUCCINOGLYCAN BIOSYNTHESIS
Erscheinungsjahr
1995
Zeitschriftentitel
GENE
Band
162
Ausgabe
1
Seite(n)
37-39
ISSN
0378-1119
Page URI
https://pub.uni-bielefeld.de/record/1640119

Zitieren

Becker A, Schmidt M, Jäger W, Pühler A. New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions. GENE. 1995;162(1):37-39.
Becker, A., Schmidt, M., Jäger, W., & Pühler, A. (1995). New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions. GENE, 162(1), 37-39. https://doi.org/10.1016/0378-1119(95)00313-U
Becker, A, Schmidt, M, Jäger, W, and Pühler, Alfred. 1995. “New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions”. GENE 162 (1): 37-39.
Becker, A., Schmidt, M., Jäger, W., and Pühler, A. (1995). New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions. GENE 162, 37-39.
Becker, A., et al., 1995. New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions. GENE, 162(1), p 37-39.
A. Becker, et al., “New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions”, GENE, vol. 162, 1995, pp. 37-39.
Becker, A., Schmidt, M., Jäger, W., Pühler, A.: New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions. GENE. 162, 37-39 (1995).
Becker, A, Schmidt, M, Jäger, W, and Pühler, Alfred. “New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions”. GENE 162.1 (1995): 37-39.

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Identification of a system that allows a Rhizobium tropici dctA mutant to grow on succinate, but not on other C4-dicarboxylates.
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Requirement of novel competence genes pilT and pilU of Pseudomonas stutzeri for natural transformation and suppression of pilT deficiency by a hexahistidine tag on the type IV pilus protein PilAI.
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A homologue of an operon required for DNA transfer in Agrobacterium is required in Brucella abortus for virulence and intracellular multiplication.
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New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon.
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The pssB gene product of Rhizobium leguminosarum bv. trifolii is homologous to a family of inositol monophosphatases.
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Phosphorylation-independent activity of the response regulators AlgB and AlgR in promoting alginate biosynthesis in mucoid Pseudomonas aeruginosa.
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Sulfation of nod factors via nodHPQ is nodD independent in Rhizobium tropici CIAT899.
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Unusual structure of the tonB-exb DNA region of Xanthomonas campestris pv. campestris: tonB, exbB, and exbD1 are essential for ferric iron uptake, but exbD2 is not.
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Promoter analysis of the Xanthomonas campestris pv. campestris gum operon directing biosynthesis of the xanthan polysaccharide.
Katzen F, Becker A, Zorreguieta A, Pühler A, Ielpi L., J Bacteriol 178(14), 1996
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Extension of the Rhizobium meliloti succinoglycan biosynthesis gene cluster: identification of the exsA gene encoding an ABC transporter protein, and the exsB gene which probably codes for a regulator of succinoglycan biosynthesis.
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