New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions

Becker A, Schmidt M, Jäger W, Pühler A (1995)
GENE 162(1): 37-39.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
A set of antibiotic-resistance and promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions was constructed. The cassettes contain the aacC1 gene of transposon Tn1696 conferring resistance to gentamicin in a large variety of Gram(-) and Gram(+) bacteria. In addition to the antibiotic-resistance gene, a promoterless Escherichia coli lacZ gene was included in the cassettes, allowing the determination of the transcriptional activity at the insertion site. The cassettes can be excised from a plasmid mediating ampicillin resistance by many commonly used restriction enzymes. The new constructs have been successfully used for mutagenesis and studies of gene transcription in Rhizobium meliloti.
Erscheinungsjahr
Zeitschriftentitel
GENE
Band
162
Ausgabe
1
Seite(n)
37-39
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Becker A, Schmidt M, Jäger W, Pühler A. New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions. GENE. 1995;162(1):37-39.
Becker, A., Schmidt, M., Jäger, W., & Pühler, A. (1995). New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions. GENE, 162(1), 37-39. doi:10.1016/0378-1119(95)00313-U
Becker, A., Schmidt, M., Jäger, W., and Pühler, A. (1995). New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions. GENE 162, 37-39.
Becker, A., et al., 1995. New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions. GENE, 162(1), p 37-39.
A. Becker, et al., “New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions”, GENE, vol. 162, 1995, pp. 37-39.
Becker, A., Schmidt, M., Jäger, W., Pühler, A.: New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions. GENE. 162, 37-39 (1995).
Becker, A, Schmidt, M, Jäger, W, and Pühler, Alfred. “New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions”. GENE 162.1 (1995): 37-39.

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