New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions

Becker A, Schmidt M, Jäger W, Pühler A (1995)
GENE 162(1): 37-39.

Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
; ; ;
Abstract / Bemerkung
A set of antibiotic-resistance and promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions was constructed. The cassettes contain the aacC1 gene of transposon Tn1696 conferring resistance to gentamicin in a large variety of Gram(-) and Gram(+) bacteria. In addition to the antibiotic-resistance gene, a promoterless Escherichia coli lacZ gene was included in the cassettes, allowing the determination of the transcriptional activity at the insertion site. The cassettes can be excised from a plasmid mediating ampicillin resistance by many commonly used restriction enzymes. The new constructs have been successfully used for mutagenesis and studies of gene transcription in Rhizobium meliloti.


Becker A, Schmidt M, Jäger W, Pühler A. New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions. GENE. 1995;162(1):37-39.
Becker, A., Schmidt, M., Jäger, W., & Pühler, A. (1995). New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions. GENE, 162(1), 37-39. doi:10.1016/0378-1119(95)00313-U
Becker, A., Schmidt, M., Jäger, W., and Pühler, A. (1995). New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions. GENE 162, 37-39.
Becker, A., et al., 1995. New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions. GENE, 162(1), p 37-39.
A. Becker, et al., “New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions”, GENE, vol. 162, 1995, pp. 37-39.
Becker, A., Schmidt, M., Jäger, W., Pühler, A.: New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions. GENE. 162, 37-39 (1995).
Becker, A, Schmidt, M, Jäger, W, and Pühler, Alfred. “New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions”. GENE 162.1 (1995): 37-39.

105 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Sinorhizobium fredii HH103 RirA Is Required for Oxidative Stress Resistance and Efficient Symbiosis with Soybean.
Crespo-Rivas JC, Navarro-Gómez P, Alias-Villegas C, Shi J, Zhen T, Niu Y, Cuéllar V, Moreno J, Cubo T, Vinardell JM, Ruiz-Sainz JE, Acosta-Jurado S, Soto MJ., Int J Mol Sci 20(3), 2019
PMID: 30759803
Herbaspirillum seropedicae Differentially Expressed Genes in Response to Iron Availability.
Trovero MF, Scavone P, Platero R, de Souza EM, Fabiano E, Rosconi F., Front Microbiol 9(), 2018
PMID: 30018605
A Cyanide-Induced 3-Cyanoalanine Nitrilase in the Cyanide-Assimilating Bacterium Pseudomonas pseudoalcaligenes Strain CECT 5344.
Acera F, Carmona MI, Castillo F, Quesada A, Blasco R., Appl Environ Microbiol 83(9), 2017
PMID: 28235872
HmuS and HmuQ of Ensifer/Sinorhizobium meliloti degrade heme in vitro and participate in heme metabolism in vivo.
Amarelle V, Rosconi F, Lázaro-Martínez JM, Buldain G, Noya F, O'Brian MR, Fabiano E., Biometals 29(2), 2016
PMID: 26906560
Spatiotemporal choreography of chromosome and megaplasmids in the Sinorhizobium meliloti cell cycle.
Frage B, Döhlemann J, Robledo M, Lucena D, Sobetzko P, Graumann PL, Becker A., Mol Microbiol 100(5), 2016
PMID: 26853523
Exopolysaccharide Production by Sinorhizobium fredii HH103 Is Repressed by Genistein in a NodD1-Dependent Manner.
Acosta-Jurado S, Navarro-Gómez P, Murdoch Pdel S, Crespo-Rivas JC, Jie S, Cuesta-Berrio L, Ruiz-Sainz JE, Rodríguez-Carvajal MÁ, Vinardell JM., PLoS One 11(8), 2016
PMID: 27486751
Pseudomonas pseudoalcaligenes CECT5344, a cyanide-degrading bacterium with by-product (polyhydroxyalkanoates) formation capacity.
Manso Cobos I, Ibáñez García MI, de la Peña Moreno F, Sáez Melero LP, Luque-Almagro VM, Castillo Rodríguez F, Roldán Ruiz MD, Prieto Jiménez MA, Moreno Vivián C., Microb Cell Fact 14(), 2015
PMID: 26055753
NopC Is a Rhizobium-Specific Type 3 Secretion System Effector Secreted by Sinorhizobium (Ensifer) fredii HH103.
Jiménez-Guerrero I, Pérez-Montaño F, Medina C, Ollero FJ, López-Baena FJ., PLoS One 10(11), 2015
PMID: 26569401
A vapBC-type toxin-antitoxin module of Sinorhizobium meliloti influences symbiotic efficiency and nodule senescence of Medicago sativa.
Lipuma J, Cinege G, Bodogai M, Oláh B, Kiers A, Endre G, Dupont L, Dusha I., Environ Microbiol 16(12), 2014
PMID: 25156344
Shewanella oneidensis Hfq promotes exponential phase growth, stationary phase culture density, and cell survival.
Brennan CM, Keane ML, Hunt TM, Goulet MT, Mazzucca NQ, Sexton Z, Mezoian T, Douglas KE, Osborn JM, Pellock BJ., BMC Microbiol 13(), 2013
PMID: 23394078
The Sinorhizobium fredii HH103 lipopolysaccharide is not only relevant at early soybean nodulation stages but also for symbiosome stability in mature nodules.
Margaret I, Lucas MM, Acosta-Jurado S, Buendía-Clavería AM, Fedorova E, Hidalgo Á, Rodríguez-Carvajal MA, Rodriguez-Navarro DN, Ruiz-Sainz JE, Vinardell JM., PLoS One 8(10), 2013
PMID: 24098345
Codon-optimized antibiotic resistance gene improves efficiency of transient transformation in Frankia.
Kucho K, Kakoi K, Yamaura M, Iwashita M, Abe M, Uchiumi T., J Biosci 38(4), 2013
PMID: 24287650
Sinorhizobium fredii HH103 does not strictly require KPS and/or EPS to nodulate Glycyrrhiza uralensis, an indeterminate nodule-forming legume.
Margaret-Oliver I, Lei W, Parada M, Rodríguez-Carvajal MA, Crespo-Rivas JC, Hidalgo Á, Gil-Serrano A, Moreno J, Rodríguez-Navarro DN, Buendía-Clavería A, Ollero J, Ruiz-Sainz JE, Vinardell JM., Arch Microbiol 194(2), 2012
PMID: 21761170
The nit1C gene cluster of Pseudomonas pseudoalcaligenes CECT5344 involved in assimilation of nitriles is essential for growth on cyanide.
Estepa J, Luque-Almagro VM, Manso I, Escribano MP, Martínez-Luque M, Castillo F, Moreno-Vivián C, Roldán MD., Environ Microbiol Rep 4(3), 2012
PMID: 23760796
Sinorhizobium fredii HH103 rkp-3 genes are required for K-antigen polysaccharide biosynthesis, affect lipopolysaccharide structure and are essential for infection of legumes forming determinate nodules.
Margaret I, Crespo-Rivas JC, Acosta-Jurado S, Buendía-Clavería AM, Cubo MT, Gil-Serrano A, Moreno J, Murdoch PS, Rodríguez-Carvajal MA, Rodríguez-Navarro DN, Ruiz-Sainz JE, Sanjuán J, Soto MJ, Vinardell JM., Mol Plant Microbe Interact 25(6), 2012
PMID: 22397406
The nodulation of alfalfa by the acid-tolerant Rhizobium sp. strain LPU83 does not require sulfated forms of lipochitooligosaccharide nodulation signals.
Torres Tejerizo G, Del Papa MF, Soria-Diaz ME, Draghi W, Lozano M, Giusti Mde L, Manyani H, Megías M, Gil Serrano A, Pühler A, Niehaus K, Lagares A, Pistorio M., J Bacteriol 193(1), 2011
PMID: 20971905
Effects of modified Phycobilin biosynthesis in the Cyanobacterium Synechococcus sp. Strain PCC 7002.
Alvey RM, Biswas A, Schluchter WM, Bryant DA., J Bacteriol 193(7), 2011
PMID: 21296968
Evolution of a divinyl chlorophyll-based photosystem in Prochlorococcus.
Ito H, Tanaka A., Proc Natl Acad Sci U S A 108(44), 2011
PMID: 22006316
Experimental evidences of pSym transfer in a native peanut-associated rhizobia.
Ibáñez F, Reinoso H, Fabra A., Microbiol Res 165(6), 2010
PMID: 19853428
Identification of the Mesorhizobium loti gene responsible for glycerophosphorylation of periplasmic cyclic beta-1,2-glucans.
Kawaharada Y, Kiyota H, Eda S, Minamisawa K, Mitsui H., FEMS Microbiol Lett 302(2), 2010
PMID: 19951365
The Sinorhizobium meliloti RNA chaperone Hfq mediates symbiosis of S. meliloti and alfalfa.
Barra-Bily L, Pandey SP, Trautwetter A, Blanco C, Walker GC., J Bacteriol 192(6), 2010
PMID: 20081033
Redirecting reductant flux into hydrogen production via metabolic engineering of fermentative carbon metabolism in a cyanobacterium.
McNeely K, Xu Y, Bennette N, Bryant DA, Dismukes GC., Appl Environ Microbiol 76(15), 2010
PMID: 20543051
The effect of acidity on the production of signal molecules by Medicago roots and their recognition by Sinorhizobium
Watkin ELJ, Mutch LA, Rome S, Reeve WG, Castelli JM, Gruchlik Y, Best WM, O'Hara GW, Howieson JG., Soil Biol Biochem 41(1), 2009
PMID: IND44142906
Role of potassium uptake systems in Sinorhizobium meliloti osmoadaptation and symbiotic performance.
Domínguez-Ferreras A, Muñoz S, Olivares J, Soto MJ, Sanjuán J., J Bacteriol 191(7), 2009
PMID: 19181803
Gene SMb21071 of plasmid pSymB is required for osmoadaptation of Sinorhizobium meliloti 1021 and is implicated in modifications of cell surface polysaccharides structure in response to hyperosmotic stress.
Reguera M, Lloret J, Margaret I, Vinardell JM, Martín M, Buendía A, Rivilla R, Ruiz-Sainz JE, Bonilla I, Bolaños L., Can J Microbiol 55(10), 2009
PMID: 19935886
Expression of small RNAs in Rhizobiales and protection of a small RNA and its degradation products by Hfq in Sinorhizobium meliloti.
Voss B, Hölscher M, Baumgarth B, Kalbfleisch A, Kaya C, Hess WR, Becker A, Evguenieva-Hackenberg E., Biochem Biophys Res Commun 390(2), 2009
PMID: 19800865
Negative regulation of quorum-sensing systems in Pseudomonas aeruginosa by ATP-dependent Lon protease.
Takaya A, Tabuchi F, Tsuchiya H, Isogai E, Yamamoto T., J Bacteriol 190(12), 2008
PMID: 18408026
Regulation and symbiotic significance of nodulation outer proteins secretion in Sinorhizobium fredii HH103.
López-Baena FJ, Vinardell JM, Pérez-Montaño F, Crespo-Rivas JC, Bellogín RA, Espuny Mdel R, Ollero FJ., Microbiology 154(pt 6), 2008
PMID: 18524937
Characterization of the Pseudomonas pseudoalcaligenes CECT5344 Cyanase, an enzyme that is not essential for cyanide assimilation.
Luque-Almagro VM, Huertas MJ, Sáez LP, Luque-Romero MM, Moreno-Vivián C, Castillo F, Roldán MD, Blasco R., Appl Environ Microbiol 74(20), 2008
PMID: 18708510
ShmR is essential for utilization of heme as a nutritional iron source in Sinorhizobium meliloti.
Amarelle V, O'Brian MR, Fabiano E., Appl Environ Microbiol 74(20), 2008
PMID: 18757569
Multiple-level regulation of genes for protocatechuate degradation in Acinetobacter baylyi includes cross-regulation.
Siehler SY, Dal S, Fischer R, Patz P, Gerischer U., Appl Environ Microbiol 73(1), 2007
PMID: 17085716
Involvement of cyclopropane fatty acids in the response of Pseudomonas putida KT2440 to freeze-drying.
Muñoz-Rojas J, Bernal P, Duque E, Godoy P, Segura A, Ramos JL., Appl Environ Microbiol 72(1), 2006
PMID: 16391080
Sinorhizobium fredii HH103 mutants affected in capsular polysaccharide (KPS) are impaired for nodulation with soybean and Cajanus cajan.
Parada M, Vinardell JM, Ollero FJ, Hidalgo A, Gutiérrez R, Buendía-Clavería AM, Lei W, Margaret I, López-Baena FJ, Gil-Serrano AM, Rodríguez-Carvajal MA, Moreno J, Ruiz-Sainz JE., Mol Plant Microbe Interact 19(1), 2006
PMID: 16404952
Role of Pseudomonas aeruginosa dinB-encoded DNA polymerase IV in mutagenesis.
Sanders LH, Rockel A, Lu H, Wozniak DJ, Sutton MD., J Bacteriol 188(24), 2006
PMID: 17041045
Uncoupling of choline-O-sulphate utilization from osmoprotection in Pseudomonas putida.
Galvão TC, de Lorenzo V, Cánovas D., Mol Microbiol 62(6), 2006
PMID: 17116241
Repeat-length-independent broad-spectrum shuffling, a novel method of generating a random chimera library in vivo.
Mori K, Mukaihara T, Uesugi Y, Iwabuchi M, Hatanaka T., Appl Environ Microbiol 71(2), 2005
PMID: 15691927
The gellan gum biosynthetic genes gelC and gelE encode two separate polypeptides homologous to the activator and the kinase domains of tyrosine autokinases.
Moreira LM, Hoffmann K, Albano H, Becker A, Niehaus K, Sá-Correia I., J Mol Microbiol Biotechnol 8(1), 2004
PMID: 15741740
Environmental modulation of the pssTNOP gene expression in Rhizobium leguminosarum bv. trifolii.
Wielbo J, Mazur A, Król JE, Marczak M, Skorupska A., Can J Microbiol 50(3), 2004
PMID: 15105887
Sinorhizobium meliloti RpoH1 is required for effective nitrogen-fixing symbiosis with alfalfa.
Mitsui H, Sato T, Sato Y, Ito N, Minamisawa K., Mol Genet Genomics 271(4), 2004
PMID: 15007732
Expression islands clustered on the symbiosis island of the Mesorhizobium loti genome.
Uchiumi T, Ohwada T, Itakura M, Mitsui H, Nukui N, Dawadi P, Kaneko T, Tabata S, Yokoyama T, Tejima K, Saeki K, Omori H, Hayashi M, Maekawa T, Sriprang R, Murooka Y, Tajima S, Simomura K, Nomura M, Suzuki A, Shimoda Y, Sioya K, Abe M, Minamisawa K., J Bacteriol 186(8), 2004
PMID: 15060047
Indene bioconversion by a toluene inducible dioxygenase of Rhodococcus sp. I24.
Priefert H, O'Brien XM, Lessard PA, Dexter AF, Choi EE, Tomic S, Nagpal G, Cho JJ, Agosto M, Yang L, Treadway SL, Tamashiro L, Wallace M, Sinskey AJ., Appl Microbiol Biotechnol 65(2), 2004
PMID: 15069586
Identification of functional mob regions in Rhizobium etli: evidence for self-transmissibility of the symbiotic plasmid pRetCFN42d.
Pérez-Mendoza D, Domínguez-Ferreras A, Muñoz S, Soto MJ, Olivares J, Brom S, Girard L, Herrera-Cervera JA, Sanjuán J., J Bacteriol 186(17), 2004
PMID: 15317780
Membrane topology analysis of cyclic glucan synthase, a virulence determinant of Brucella abortus.
Ciocchini AE, Roset MS, Iñón de Iannino N, Ugalde RA., J Bacteriol 186(21), 2004
PMID: 15489431
Integration host factor is involved in transcriptional regulation of the Brucella abortus virB operon.
Sieira R, Comerci DJ, Pietrasanta LI, Ugalde RA., Mol Microbiol 54(3), 2004
PMID: 15491369
The Sinorhizobium meliloti glycine betaine biosynthetic genes (betlCBA) are induced by choline and highly expressed in bacteroids.
Mandon K, Osterås M, Boncompagni E, Trinchant JC, Spennato G, Poggi MC, Le Rudulier D., Mol Plant Microbe Interact 16(8), 2003
PMID: 12906115
Rhizobium leguminosarum bv. trifolii PssP protein is required for exopolysaccharide biosynthesis and polymerization.
Mazur A, Król JE, Wielbo J, Urbanik-Sypniewska T, Skorupska A., Mol Plant Microbe Interact 15(4), 2002
PMID: 12026178
A LuxR homolog controls production of symbiotically active extracellular polysaccharide II by Sinorhizobium meliloti.
Pellock BJ, Teplitski M, Boinay RP, Bauer WD, Walker GC., J Bacteriol 184(18), 2002
PMID: 12193623
Analysis of the zwf-pgl-eda-operon in Pseudomonas putida strains H and KT2440.
Petruschka L, Adolf K, Burchhardt G, Dernedde J, Jürgensen J, Herrmann H., FEMS Microbiol Lett 215(1), 2002
PMID: 12393206
The chvH locus of Agrobacterium encodes a homologue of an elongation factor involved in protein synthesis.
Peng WT, Banta LM, Charles TC, Nester EW., J Bacteriol 183(1), 2001
PMID: 11114898
Early symbiotic responses induced by Sinorhizobium meliloti iIvC mutants in alfalfa.
López JC, Grasso DH, Frugier F, Crespi MD, Aguilar OM., Mol Plant Microbe Interact 14(1), 2001
PMID: 11194871
Genetic characterization of a Sinorhizobium meliloti chromosomal region in lipopolysaccharide biosynthesis.
Lagares A, Hozbor DF, Niehaus K, Otero AJ, Lorenzen J, Arnold W, Pühler A., J Bacteriol 183(4), 2001
PMID: 11157937
Identification of a system that allows a Rhizobium tropici dctA mutant to grow on succinate, but not on other C4-dicarboxylates.
Batista S, Catalán AI, Hernández-Lucas I, Martínez-Romero E, Aguilar OM, Martínez-Drets G., Can J Microbiol 47(6), 2001
PMID: 11467726
Regulation of nod factor sulphation genes in Rhizobium tropici CIAT899.
Manyani H, Sousa C, Soria Díaz ME, Gil-Serrano A, Megías M., Can J Microbiol 47(6), 2001
PMID: 11467733
Sinorhizobium meliloti putA gene regulation: a new model within the family Rhizobiaceae.
Soto MJ, Jiménez-Zurdo JI, van Dillewijn P, Toro N., J Bacteriol 182(7), 2000
PMID: 10715000
Sulfation of nod factors via nodHPQ is nodD independent in Rhizobium tropici CIAT899.
Folch-Mallol JL, Manyani H, Marroquí S, Sousa C, Vargas C, Nava N, Colmenero-Flores JM, Quinto C, Megías M., Mol Plant Microbe Interact 11(10), 1998
PMID: 9768515
Molecular characterization of pssCDE genes of Rhizobium leguminosarum bv. trifolii strain TA1: pssD mutant is affected in exopolysaccharide synthesis and endocytosis of bacteria.
Król J, Wielbo J, Mazur A, Kopcińska J, Lotocka B, Golinowski W, Skorupska A., Mol Plant Microbe Interact 11(11), 1998
PMID: 9805402
The 32-kilobase exp gene cluster of Rhizobium meliloti directing the biosynthesis of galactoglucan: genetic organization and properties of the encoded gene products.
Becker A, Rüberg S, Küster H, Roxlau AA, Keller M, Ivashina T, Cheng HP, Walker GC, Pühler A., J Bacteriol 179(4), 1997
PMID: 9023225
Promoter analysis of the Xanthomonas campestris pv. campestris gum operon directing biosynthesis of the xanthan polysaccharide.
Katzen F, Becker A, Zorreguieta A, Pühler A, Ielpi L., J Bacteriol 178(14), 1996
PMID: 8763965

10 References

Daten bereitgestellt von Europe PubMed Central.

Analysis of the Rhizobium meliloti genes exoU, exoV, exoW, exoT and exoI involved in exopolysaccharide biosynthesis and nodule invasion: ExoU and ExoW resemble polysaccharide polymerising enzymes
Becker, Mol. Plant-Microb. Interact. 6(), 1993

Miller, 1972
Use of lacZ expression to monitor transcription.
Tomich CS, Kaytes PS, Olsen MK, Patel H., Plasmid 20(2), 1988
PMID: 3148948
On the evolution of Tn21-like multiresistance transposons: sequence analysis of the gene (aacC1) for gentamicin acetyltransferase-3-I(AAC(3)-I), another member of the Tn21-based expression cassette.
Wohlleben W, Arnold W, Bissonnette L, Pelletier A, Tanguay A, Roy PH, Gamboa GC, Barry GF, Aubert E, Davies J., Mol. Gen. Genet. 217(2-3), 1989
PMID: 2549372


Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®


PMID: 7557413
PubMed | Europe PMC

Suchen in

Google Scholar