Characterization of two bioluminescent Rhizobium meliloti strains constructed for field releases
Dammann-Kalinowski T, Niemann S, Keller M, Selbitschka W, Tebbe CC, Pühler A (1996)
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 45(4): 509-512.
Zeitschriftenaufsatz
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Autor*in
Dammann-Kalinowski, T;
Niemann, S;
Keller, M;
Selbitschka, WernerUniBi;
Tebbe, CC;
Pühler, AlfredUniBi 
Einrichtung
Abstract / Bemerkung
The deliberate release of genetically engineered microorganisms requires a thorough characterization of the microbes in question. For the two bioluminescent Rhizobium meliloti strains, L1 and L33 [Selbitschka et al. (1992) Mol Ecol 1:9-19; Selbitschka et al. (1995) FEMS Microbiol Ecol 16:223-232], designated for field release, the sites of genetic modifications in the chromosomes were sequenced from amplified genomic DNA. This indicated no unexpected alterations in the nucleotide sequence. The bioluminescent phenotype was stably inherited over more than 100 generations in liquid cultures. The presence of the luciferase gene in both strains did not have secondary effects on a variety of metabolic pathways as assessed by the Biolog GN system. A specific polymerase chain reaction amplification, based on the chromosomal insertion site of the luc cassette, allowed the discrimination between the two strains and thus simplifies monitoring. The RecA-deficient strain L1 showed a strongly (more than 90%) reduced ability to nodulate alfalfa in competition with its parent strain R. meliloti 2011 and its RecA(+) counterpart L33.
Erscheinungsjahr
1996
Zeitschriftentitel
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Band
45
Ausgabe
4
Seite(n)
509-512
ISSN
0175-7598
Page URI
https://pub.uni-bielefeld.de/record/1639079
Zitieren
Dammann-Kalinowski T, Niemann S, Keller M, Selbitschka W, Tebbe CC, Pühler A. Characterization of two bioluminescent Rhizobium meliloti strains constructed for field releases. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 1996;45(4):509-512.
Dammann-Kalinowski, T., Niemann, S., Keller, M., Selbitschka, W., Tebbe, C. C., & Pühler, A. (1996). Characterization of two bioluminescent Rhizobium meliloti strains constructed for field releases. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 45(4), 509-512.
Dammann-Kalinowski, T, Niemann, S, Keller, M, Selbitschka, Werner, Tebbe, CC, and Pühler, Alfred. 1996. “Characterization of two bioluminescent Rhizobium meliloti strains constructed for field releases”. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 45 (4): 509-512.
Dammann-Kalinowski, T., Niemann, S., Keller, M., Selbitschka, W., Tebbe, C. C., and Pühler, A. (1996). Characterization of two bioluminescent Rhizobium meliloti strains constructed for field releases. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 45, 509-512.
Dammann-Kalinowski, T., et al., 1996. Characterization of two bioluminescent Rhizobium meliloti strains constructed for field releases. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 45(4), p 509-512.
T. Dammann-Kalinowski, et al., “Characterization of two bioluminescent Rhizobium meliloti strains constructed for field releases”, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 45, 1996, pp. 509-512.
Dammann-Kalinowski, T., Niemann, S., Keller, M., Selbitschka, W., Tebbe, C.C., Pühler, A.: Characterization of two bioluminescent Rhizobium meliloti strains constructed for field releases. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 45, 509-512 (1996).
Dammann-Kalinowski, T, Niemann, S, Keller, M, Selbitschka, Werner, Tebbe, CC, and Pühler, Alfred. “Characterization of two bioluminescent Rhizobium meliloti strains constructed for field releases”. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 45.4 (1996): 509-512.
7 Zitationen in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
Soil factors exhibit greater influence than bacterial inoculation on alfalfa growth and nitrogen fixation.
Neumann U, Kosier B, Jahnke J, Priefer UB, Al-Halbouni D., FEMS Microbiol Ecol 77(3), 2011
PMID: 21627671
Neumann U, Kosier B, Jahnke J, Priefer UB, Al-Halbouni D., FEMS Microbiol Ecol 77(3), 2011
PMID: 21627671
Long-term field release of bioluminescent Sinorhizobium meliloti strains to assess the influence of a recA mutation on the strains' survival.
Selbitschka W, Keller M, Miethling-Graff R, Dresing U, Schwieger F, Krahn I, Homann I, Dammann-Kalinowski T, Pühler A, Tebbe CC., Microb Ecol 52(3), 2006
PMID: 16924432
Selbitschka W, Keller M, Miethling-Graff R, Dresing U, Schwieger F, Krahn I, Homann I, Dammann-Kalinowski T, Pühler A, Tebbe CC., Microb Ecol 52(3), 2006
PMID: 16924432
Diversity of Sinorhizobium meliloti from the Central Asian Alfalfa Gene Center.
Roumiantseva ML, Andronov EE, Sharypova LA, Dammann-Kalinowski T, Keller M, Young JP, Simarov BV., Appl Environ Microbiol 68(9), 2002
PMID: 12200335
Roumiantseva ML, Andronov EE, Sharypova LA, Dammann-Kalinowski T, Keller M, Young JP, Simarov BV., Appl Environ Microbiol 68(9), 2002
PMID: 12200335
Effect of field inoculation with Sinorhizobium meliloti L33 on the composition of bacterial communities in rhizospheres of a target plant (Medicago sativa) and a non-target plant (Chenopodium album)-linking of 16S rRNA gene-based single-strand conformation polymorphism community profiles to the diversity of cultivated bacteria.
Schwieger F, Tebbe CC., Appl Environ Microbiol 66(8), 2000
PMID: 10919821
Schwieger F, Tebbe CC., Appl Environ Microbiol 66(8), 2000
PMID: 10919821
Intergeneric transfer of conjugative and mobilizable plasmids harbored by Escherichia coli in the gut of the soil microarthropod Folsomia candida (Collembola).
Hoffmann A, Thimm T, Dröge M, Moore ER, Munch JC, Tebbe CC., Appl Environ Microbiol 64(7), 1998
PMID: 9647844
Hoffmann A, Thimm T, Dröge M, Moore ER, Munch JC, Tebbe CC., Appl Environ Microbiol 64(7), 1998
PMID: 9647844
Reduced survival of a RecA-deficient Sinorhizobium meliloti strain in sterile and non-sterile during heat stress
Dresing U, Hagen M, Selbitschka W, Puhler A, Keller M., FEMS Microbiol Ecol 27(4), 1998
PMID: IND21976940
Dresing U, Hagen M, Selbitschka W, Puhler A, Keller M., FEMS Microbiol Ecol 27(4), 1998
PMID: IND21976940
Evaluation of the recA-based containment system in Rhizobium meliloti GR4
Herrera-Cervera JA, Rodriguez-Alonso FI, Olivares J, Sanjuan J., FEMS Microbiol Ecol 22(1), 1997
PMID: IND20576589
Herrera-Cervera JA, Rodriguez-Alonso FI, Olivares J, Sanjuan J., FEMS Microbiol Ecol 22(1), 1997
PMID: IND20576589
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