Male sterility in transgenic tobacco plants induced by tapetum-specific deacetylation of the externally applied non-toxic compound N-acetyl-L-phosphinothricin

Kriete G, Niehaus K, Perlick AM, Pühler A, Broer I (1996)
PLANT JOURNAL 9(6): 809-818.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Kriete, G; Niehaus, KarstenUniBi; Perlick, AM; Pühler, AlfredUniBi ; Broer, I
Abstract / Bemerkung
A system for the inducible destruction of plant tissues based on the deacetylation of the non-toxic compound N-acetyl-L-phosphinothricin (N-ac-Pt) has been developed. The argE gene product of Escherichia coli, representing a N-acetyl-L-ornithine deacetylase was identified to remove the acetyl-group from N-ac-Pt giving the cytotoxic compound L-phosphinothricin (Pt, glufosinate). Transgenic Nicotiana tabacum plants constitutively expressing the argE gene were constructed. No effect of the bacterial N-acetyl-L-ornithine deacetylase on plant growth and reproduction could be traced. However, application of N-ac-Pt on leaves of the transgenic plants led to the formation of necrotic areas due to the release of Pt. Additionally, due to the uptake of the N-ac-Pt by roots, transgenic shoots grown on medium containing N-ac-Pt bleached within 6-7 days and finally died. Untransformed controls showed no reaction to high amounts of N-ac-Pt applied, either under sterile or under unsterile conditions. In order to construct inducible male-sterile plants, the argE coding region was fused to a DNA fragment carrying sequences homologous to the tobacco TA29 promoter, known to function exclusively in the tapetum. Owing to the tapetum-specific expression of the chimeric gene the application of N-ac-Pt led to empty anthers resulting in male-sterile plants. The sanity of the female reproductive part of the male-sterile flowers could be demonstrated by cross-pollination, Without N-ac-Pt treatment the plants turned out to be completely fertile making fertility restoration in the F-1 generation superflous. The system presented is easy to handle and might be applicable to a wide range of crop plants.
Erscheinungsjahr
1996
Zeitschriftentitel
PLANT JOURNAL
Band
9
Ausgabe
6
Seite(n)
809-818
ISSN
0960-7412
eISSN
1365-313X
Page URI
https://pub.uni-bielefeld.de/record/1639029

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Kriete G, Niehaus K, Perlick AM, Pühler A, Broer I. Male sterility in transgenic tobacco plants induced by tapetum-specific deacetylation of the externally applied non-toxic compound N-acetyl-L-phosphinothricin. PLANT JOURNAL. 1996;9(6):809-818.
Kriete, G., Niehaus, K., Perlick, A. M., Pühler, A., & Broer, I. (1996). Male sterility in transgenic tobacco plants induced by tapetum-specific deacetylation of the externally applied non-toxic compound N-acetyl-L-phosphinothricin. PLANT JOURNAL, 9(6), 809-818. doi:10.1046/j.1365-313X.1996.9060809.x
Kriete, G., Niehaus, K., Perlick, A. M., Pühler, A., and Broer, I. (1996). Male sterility in transgenic tobacco plants induced by tapetum-specific deacetylation of the externally applied non-toxic compound N-acetyl-L-phosphinothricin. PLANT JOURNAL 9, 809-818.
Kriete, G., et al., 1996. Male sterility in transgenic tobacco plants induced by tapetum-specific deacetylation of the externally applied non-toxic compound N-acetyl-L-phosphinothricin. PLANT JOURNAL, 9(6), p 809-818.
G. Kriete, et al., “Male sterility in transgenic tobacco plants induced by tapetum-specific deacetylation of the externally applied non-toxic compound N-acetyl-L-phosphinothricin”, PLANT JOURNAL, vol. 9, 1996, pp. 809-818.
Kriete, G., Niehaus, K., Perlick, A.M., Pühler, A., Broer, I.: Male sterility in transgenic tobacco plants induced by tapetum-specific deacetylation of the externally applied non-toxic compound N-acetyl-L-phosphinothricin. PLANT JOURNAL. 9, 809-818 (1996).
Kriete, G, Niehaus, Karsten, Perlick, AM, Pühler, Alfred, and Broer, I. “Male sterility in transgenic tobacco plants induced by tapetum-specific deacetylation of the externally applied non-toxic compound N-acetyl-L-phosphinothricin”. PLANT JOURNAL 9.6 (1996): 809-818.

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PMID: 30138445
Molecular Approaches for Manipulating Male Sterility and Strategies for Fertility Restoration in Plants.
Shukla P, Singh NK, Gautam R, Ahmed I, Yadav D, Sharma A, Kirti PB., Mol Biotechnol 59(9-10), 2017
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Application of rice microspore-preferred promoters to manipulate early pollen development in Arabidopsis: a heterologous system.
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PMID: 24615687
Temporal and spatial control of gene expression in horticultural crops.
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PMID: 26504550
Reversible male sterility in eggplant (Solanum melongena L.) by artificial microRNA-mediated silencing of general transcription factor genes.
Toppino L, Kooiker M, Lindner M, Dreni L, Rotino GL, Kater MM., Plant Biotechnol J 9(6), 2011
PMID: 20955179
D-glufosinate as a male sterility agent for hybrid seed production.
Hawkes T, Pline-Srnic W, Dale R, Friend E, Hollinshead T, Howe P, Thompson P, Viner R, Greenland A., Plant Biotechnol J 9(3), 2011
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Pollination control technologies for hybrid breeding
Kempe Katja, Gils Mario., Mol Breed 27(4), 2011
PMID: IND44515010
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PMID: 21743085
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Wang Yufeng, Zha Xiaojun, Zhang Shiyong, Qian Xiaoyin, Dong Xianxin, Sun Fan, Yang Jinshui., Plant Sci 178(2), 2010
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Death of a tapetum: A programme of developmental altruism
Parish RogerW, Li SongF., Plant Sci 178(2), 2010
PMID: IND44323488
A novel herbicide-inducible male sterility system.
Zhang J, Zhang W, Yen Y, Long H, Deng G, Pan Z, Yu M., J Sci Food Agric 90(14), 2010
PMID: 20824679
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Zhang Jinhui, Zhang Wenlu, Yen Yang, Long Hai, Deng Guangbing, Pan Zhifen, Yu Maoqun., J Sci Food Agric 90(14), 2010
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PMID: IND44149067
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Nizampatnam NR, Doodhi H, Kalinati Narasimhan Y, Mulpuri S, Viswanathaswamy DK., Planta 229(4), 2009
PMID: 19151958
Rapeseed cytoplasm gives advantage in wild relatives and complicates genetically modified crop biocontainment.
Allainguillaume J, Harwood T, Ford CS, Cuccato G, Norris C, Allender CJ, Welters R, King GJ, Wilkinson MJ., New Phytol 183(4), 2009
PMID: 19496946
Suppression and restoration of male fertility using a transcription factor.
Li SF, Iacuone S, Parish RW., Plant Biotechnol J 5(2), 2007
PMID: 17309685
RNAi-mediated male sterility of tobacco by silencing TA29.
Nawaz-ul-Rehman MS, Mansoor S, Khan AA, Zafar Y, Briddon RW., Mol Biotechnol 36(2), 2007
PMID: 17914195
Stable male sterility induced by the expression of mutated melon ethylene receptor genes in Nicotiana tabacum
Ishimaru K, Takada K, Watanabe S, Kamada H, Ezura H., Plant Sci 171(3), 2006
PMID: IND43821112
Selective elimination of perennial ryegrass by activation of a pro-herbicide through engineering E. coli argE gene.
Chen X, Yang W, Sivamani E, Bruneau AH, Wang B, Qu R., Mol Breed 15(4), 2005
PMID: IND43789627
Hybrid seed production and the challenge of propagating male-sterile plants.
Perez-Prat E, van Lookeren Campagne MM., Trends Plant Sci 7(5), 2002
PMID: 11992824
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Sa G, Mi M, He-Chun Y, Guo-Feng L., Transgenic Res 11(3), 2002
PMID: 12113459
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Burgess DG, Ralston EJ, Hanson WG, Heckert M, Ho M, Jenq T, Palys JM, Tang K, Gutterson N., Plant J 31(1), 2002
PMID: 12100487

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